Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/213555 |
Resumo: | Objective: This study aimed to examine changes in miRNAs expression profile of COPD patients. Methods: Thirty-six COPD patients as well as thirty-three healthy volunteers were recruited. Total RNAs were collected from the plasma of each participant. The differentially expressed miRNAs in COPD were screened from the GEO database. RT-qPCR was carried out to detect miRNA expression. Results: In total, 9 out of 55 miRNAs were expressed differentially in COPD patients. Confirmed by RT-qPCR validation, 6 miRNAs increased while 3 miRNAs decreased. Further analysis of miR-423-5p, which has not been reported in COPD, showed that AUC for the diagnosis of COPD was 0.9651, and miR-423-5p levels was inversely correlated with the duration of smoking. Conclusion: The present study demonstrates that miR-423-5p is a potential marker for identifying COPD patients. |
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Clinics |
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Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patientsCOPDmiR-423-5pGEOReceiver operating curveObjective: This study aimed to examine changes in miRNAs expression profile of COPD patients. Methods: Thirty-six COPD patients as well as thirty-three healthy volunteers were recruited. Total RNAs were collected from the plasma of each participant. The differentially expressed miRNAs in COPD were screened from the GEO database. RT-qPCR was carried out to detect miRNA expression. Results: In total, 9 out of 55 miRNAs were expressed differentially in COPD patients. Confirmed by RT-qPCR validation, 6 miRNAs increased while 3 miRNAs decreased. Further analysis of miR-423-5p, which has not been reported in COPD, showed that AUC for the diagnosis of COPD was 0.9651, and miR-423-5p levels was inversely correlated with the duration of smoking. Conclusion: The present study demonstrates that miR-423-5p is a potential marker for identifying COPD patients.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2022-09-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/21355510.1016/j.clinsp.2022.100102Clinics; Vol. 77 (2022); 100102Clinics; v. 77 (2022); 100102Clinics; Vol. 77 (2022); 1001021980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/213555/195639Copyright (c) 2023 Clinicsinfo:eu-repo/semantics/openAccessZhang, XinShi, QingXiong, LuShi, ShiyeLi, YongWang, YanhuanZhang, Mingchuan2023-07-06T13:04:59Zoai:revistas.usp.br:article/213555Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2023-07-06T13:04:59Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients |
title |
Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients |
spellingShingle |
Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients Zhang, Xin COPD miR-423-5p GEO Receiver operating curve |
title_short |
Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients |
title_full |
Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients |
title_fullStr |
Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients |
title_full_unstemmed |
Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients |
title_sort |
Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients |
author |
Zhang, Xin |
author_facet |
Zhang, Xin Shi, Qing Xiong, Lu Shi, Shiye Li, Yong Wang, Yanhuan Zhang, Mingchuan |
author_role |
author |
author2 |
Shi, Qing Xiong, Lu Shi, Shiye Li, Yong Wang, Yanhuan Zhang, Mingchuan |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Zhang, Xin Shi, Qing Xiong, Lu Shi, Shiye Li, Yong Wang, Yanhuan Zhang, Mingchuan |
dc.subject.por.fl_str_mv |
COPD miR-423-5p GEO Receiver operating curve |
topic |
COPD miR-423-5p GEO Receiver operating curve |
description |
Objective: This study aimed to examine changes in miRNAs expression profile of COPD patients. Methods: Thirty-six COPD patients as well as thirty-three healthy volunteers were recruited. Total RNAs were collected from the plasma of each participant. The differentially expressed miRNAs in COPD were screened from the GEO database. RT-qPCR was carried out to detect miRNA expression. Results: In total, 9 out of 55 miRNAs were expressed differentially in COPD patients. Confirmed by RT-qPCR validation, 6 miRNAs increased while 3 miRNAs decreased. Further analysis of miR-423-5p, which has not been reported in COPD, showed that AUC for the diagnosis of COPD was 0.9651, and miR-423-5p levels was inversely correlated with the duration of smoking. Conclusion: The present study demonstrates that miR-423-5p is a potential marker for identifying COPD patients. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-23 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/213555 10.1016/j.clinsp.2022.100102 |
url |
https://www.revistas.usp.br/clinics/article/view/213555 |
identifier_str_mv |
10.1016/j.clinsp.2022.100102 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/213555/195639 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Clinics info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Clinics |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 77 (2022); 100102 Clinics; v. 77 (2022); 100102 Clinics; Vol. 77 (2022); 100102 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222766978301952 |