Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients

Detalhes bibliográficos
Autor(a) principal: Zhang, Xin
Data de Publicação: 2022
Outros Autores: Shi, Qing, Xiong, Lu, Shi, Shiye, Li, Yong, Wang, Yanhuan, Zhang, Mingchuan
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/213555
Resumo: Objective: This study aimed to examine changes in miRNAs expression profile of COPD patients. Methods: Thirty-six COPD patients as well as thirty-three healthy volunteers were recruited. Total RNAs were collected from the plasma of each participant. The differentially expressed miRNAs in COPD were screened from the GEO database. RT-qPCR was carried out to detect miRNA expression. Results: In total, 9 out of 55 miRNAs were expressed differentially in COPD patients. Confirmed by RT-qPCR validation, 6 miRNAs increased while 3 miRNAs decreased. Further analysis of miR-423-5p, which has not been reported in COPD, showed that AUC for the diagnosis of COPD was 0.9651, and miR-423-5p levels was inversely correlated with the duration of smoking. Conclusion: The present study demonstrates that miR-423-5p is a potential marker for identifying COPD patients.
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spelling Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patientsCOPDmiR-423-5pGEOReceiver operating curveObjective: This study aimed to examine changes in miRNAs expression profile of COPD patients. Methods: Thirty-six COPD patients as well as thirty-three healthy volunteers were recruited. Total RNAs were collected from the plasma of each participant. The differentially expressed miRNAs in COPD were screened from the GEO database. RT-qPCR was carried out to detect miRNA expression. Results: In total, 9 out of 55 miRNAs were expressed differentially in COPD patients. Confirmed by RT-qPCR validation, 6 miRNAs increased while 3 miRNAs decreased. Further analysis of miR-423-5p, which has not been reported in COPD, showed that AUC for the diagnosis of COPD was 0.9651, and miR-423-5p levels was inversely correlated with the duration of smoking. Conclusion: The present study demonstrates that miR-423-5p is a potential marker for identifying COPD patients.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2022-09-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/21355510.1016/j.clinsp.2022.100102Clinics; Vol. 77 (2022); 100102Clinics; v. 77 (2022); 100102Clinics; Vol. 77 (2022); 1001021980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/213555/195639Copyright (c) 2023 Clinicsinfo:eu-repo/semantics/openAccessZhang, XinShi, QingXiong, LuShi, ShiyeLi, YongWang, YanhuanZhang, Mingchuan2023-07-06T13:04:59Zoai:revistas.usp.br:article/213555Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2023-07-06T13:04:59Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients
title Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients
spellingShingle Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients
Zhang, Xin
COPD
miR-423-5p
GEO
Receiver operating curve
title_short Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients
title_full Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients
title_fullStr Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients
title_full_unstemmed Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients
title_sort Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients
author Zhang, Xin
author_facet Zhang, Xin
Shi, Qing
Xiong, Lu
Shi, Shiye
Li, Yong
Wang, Yanhuan
Zhang, Mingchuan
author_role author
author2 Shi, Qing
Xiong, Lu
Shi, Shiye
Li, Yong
Wang, Yanhuan
Zhang, Mingchuan
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Zhang, Xin
Shi, Qing
Xiong, Lu
Shi, Shiye
Li, Yong
Wang, Yanhuan
Zhang, Mingchuan
dc.subject.por.fl_str_mv COPD
miR-423-5p
GEO
Receiver operating curve
topic COPD
miR-423-5p
GEO
Receiver operating curve
description Objective: This study aimed to examine changes in miRNAs expression profile of COPD patients. Methods: Thirty-six COPD patients as well as thirty-three healthy volunteers were recruited. Total RNAs were collected from the plasma of each participant. The differentially expressed miRNAs in COPD were screened from the GEO database. RT-qPCR was carried out to detect miRNA expression. Results: In total, 9 out of 55 miRNAs were expressed differentially in COPD patients. Confirmed by RT-qPCR validation, 6 miRNAs increased while 3 miRNAs decreased. Further analysis of miR-423-5p, which has not been reported in COPD, showed that AUC for the diagnosis of COPD was 0.9651, and miR-423-5p levels was inversely correlated with the duration of smoking. Conclusion: The present study demonstrates that miR-423-5p is a potential marker for identifying COPD patients.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-23
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/213555
10.1016/j.clinsp.2022.100102
url https://www.revistas.usp.br/clinics/article/view/213555
identifier_str_mv 10.1016/j.clinsp.2022.100102
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/213555/195639
dc.rights.driver.fl_str_mv Copyright (c) 2023 Clinics
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Clinics
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 77 (2022); 100102
Clinics; v. 77 (2022); 100102
Clinics; Vol. 77 (2022); 100102
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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