Effects of different peep levels on mesenteric leukocyte-endothelial interactions in rats during mechanical ventilation
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/18037 |
Resumo: | INTRODUCTION: Mechanical ventilation with positive end expiratory pressure (PEEP) improves oxygenation and treats acute pulmonary failure. However, increased intrathoracic pressure may cause regional blood flow alterations that may contribute to mesenteric ischemia and gastrointestinal failure. We investigated the effects of different PEEP levels on mesenteric leukocyte-endothelial interactions. METHODS: Forty-four male Wistar rats were initially anesthetized (Pentobarbital I.P. 50mg/kg) and randomly assigned to one of the following groups: 1) NAIVE (only anesthesia; n=9), 2) PEEP 0 (PEEP of 0 cmH2O, n=13), 3) PEEP 5 (PEEP of 5 cmH2O, n=12), and 4) PEEP 10 (PEEP of 10 cmH2O, n=13). Positive end expiratory pressure groups were tracheostomized and mechanically ventilated with a tidal volume of 10 mL/kg, respiratory rate of 70 rpm, and inspired oxygen fraction of 1. Animals were maintained under isoflurane anesthesia. After two hours, laparotomy was performed, and leukocyte-endothelial interactions were evaluated by intravital microscopy. RESULTS: No significant changes were observed in mean arterial blood pressure among groups during the study. Tracheal peak pressure was smaller in PEEP 5 compared with PEEP 0 and PEEP 10 groups (11, 15, and 16 cmH2O, respectively; p |
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Clinics |
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Effects of different peep levels on mesenteric leukocyte-endothelial interactions in rats during mechanical ventilation Mechanical ventilationLeukocyte-endothelial interactionsMicrocirculationMesentery INTRODUCTION: Mechanical ventilation with positive end expiratory pressure (PEEP) improves oxygenation and treats acute pulmonary failure. However, increased intrathoracic pressure may cause regional blood flow alterations that may contribute to mesenteric ischemia and gastrointestinal failure. We investigated the effects of different PEEP levels on mesenteric leukocyte-endothelial interactions. METHODS: Forty-four male Wistar rats were initially anesthetized (Pentobarbital I.P. 50mg/kg) and randomly assigned to one of the following groups: 1) NAIVE (only anesthesia; n=9), 2) PEEP 0 (PEEP of 0 cmH2O, n=13), 3) PEEP 5 (PEEP of 5 cmH2O, n=12), and 4) PEEP 10 (PEEP of 10 cmH2O, n=13). Positive end expiratory pressure groups were tracheostomized and mechanically ventilated with a tidal volume of 10 mL/kg, respiratory rate of 70 rpm, and inspired oxygen fraction of 1. Animals were maintained under isoflurane anesthesia. After two hours, laparotomy was performed, and leukocyte-endothelial interactions were evaluated by intravital microscopy. RESULTS: No significant changes were observed in mean arterial blood pressure among groups during the study. Tracheal peak pressure was smaller in PEEP 5 compared with PEEP 0 and PEEP 10 groups (11, 15, and 16 cmH2O, respectively; pHospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2009-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1803710.1590/S1807-59322009000500012Clinics; Vol. 64 No. 5 (2009); 443-450 Clinics; v. 64 n. 5 (2009); 443-450 Clinics; Vol. 64 Núm. 5 (2009); 443-450 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/18037/20102Aikawa, PriscilaFarsky, Sandra Helena PoliselliOliveira, Maria Aparecida dePazetti, RogérioMauad, ThaísSannomiya, PaulinaNakagawa, Naomi Kondoinfo:eu-repo/semantics/openAccess2012-05-22T18:52:31Zoai:revistas.usp.br:article/18037Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-22T18:52:31Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Effects of different peep levels on mesenteric leukocyte-endothelial interactions in rats during mechanical ventilation |
title |
Effects of different peep levels on mesenteric leukocyte-endothelial interactions in rats during mechanical ventilation |
spellingShingle |
Effects of different peep levels on mesenteric leukocyte-endothelial interactions in rats during mechanical ventilation Aikawa, Priscila Mechanical ventilation Leukocyte-endothelial interactions Microcirculation Mesentery |
title_short |
Effects of different peep levels on mesenteric leukocyte-endothelial interactions in rats during mechanical ventilation |
title_full |
Effects of different peep levels on mesenteric leukocyte-endothelial interactions in rats during mechanical ventilation |
title_fullStr |
Effects of different peep levels on mesenteric leukocyte-endothelial interactions in rats during mechanical ventilation |
title_full_unstemmed |
Effects of different peep levels on mesenteric leukocyte-endothelial interactions in rats during mechanical ventilation |
title_sort |
Effects of different peep levels on mesenteric leukocyte-endothelial interactions in rats during mechanical ventilation |
author |
Aikawa, Priscila |
author_facet |
Aikawa, Priscila Farsky, Sandra Helena Poliselli Oliveira, Maria Aparecida de Pazetti, Rogério Mauad, Thaís Sannomiya, Paulina Nakagawa, Naomi Kondo |
author_role |
author |
author2 |
Farsky, Sandra Helena Poliselli Oliveira, Maria Aparecida de Pazetti, Rogério Mauad, Thaís Sannomiya, Paulina Nakagawa, Naomi Kondo |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Aikawa, Priscila Farsky, Sandra Helena Poliselli Oliveira, Maria Aparecida de Pazetti, Rogério Mauad, Thaís Sannomiya, Paulina Nakagawa, Naomi Kondo |
dc.subject.por.fl_str_mv |
Mechanical ventilation Leukocyte-endothelial interactions Microcirculation Mesentery |
topic |
Mechanical ventilation Leukocyte-endothelial interactions Microcirculation Mesentery |
description |
INTRODUCTION: Mechanical ventilation with positive end expiratory pressure (PEEP) improves oxygenation and treats acute pulmonary failure. However, increased intrathoracic pressure may cause regional blood flow alterations that may contribute to mesenteric ischemia and gastrointestinal failure. We investigated the effects of different PEEP levels on mesenteric leukocyte-endothelial interactions. METHODS: Forty-four male Wistar rats were initially anesthetized (Pentobarbital I.P. 50mg/kg) and randomly assigned to one of the following groups: 1) NAIVE (only anesthesia; n=9), 2) PEEP 0 (PEEP of 0 cmH2O, n=13), 3) PEEP 5 (PEEP of 5 cmH2O, n=12), and 4) PEEP 10 (PEEP of 10 cmH2O, n=13). Positive end expiratory pressure groups were tracheostomized and mechanically ventilated with a tidal volume of 10 mL/kg, respiratory rate of 70 rpm, and inspired oxygen fraction of 1. Animals were maintained under isoflurane anesthesia. After two hours, laparotomy was performed, and leukocyte-endothelial interactions were evaluated by intravital microscopy. RESULTS: No significant changes were observed in mean arterial blood pressure among groups during the study. Tracheal peak pressure was smaller in PEEP 5 compared with PEEP 0 and PEEP 10 groups (11, 15, and 16 cmH2O, respectively; p |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-05-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/18037 10.1590/S1807-59322009000500012 |
url |
https://www.revistas.usp.br/clinics/article/view/18037 |
identifier_str_mv |
10.1590/S1807-59322009000500012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/18037/20102 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 64 No. 5 (2009); 443-450 Clinics; v. 64 n. 5 (2009); 443-450 Clinics; Vol. 64 Núm. 5 (2009); 443-450 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222754756100096 |