Capture erytroimmunoadsorption test for neurocysticercosis immunodiagnosis

• Autor(a) principal: Pialarissi, Carmen Silvia de M.
• Data de Publicação: 1994
• Outros Autores: Nitrini, Sandra Maria Ottati de Oliveira
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista de Saúde Pública
• Texto Completo: https://www.revistas.usp.br/rsp/article/view/24019
• topic: Cisticercose^i1^sdiagnóst; Técnicas de imunoadsorção; Anticorpos anti-helmintos^i1^sles; Cysticercosis^i2^sdiagno; Immunosorbent techniques; Antibodies; helminth^i2^scerebrospinal fl

The capture erytroimmunoadsorption (C-EIA) test was standardized for detection of Taenia solium cysticercus-IgG specific antibodies in cerebrospinal fluid (CSF) from patients with ncurocysticercosis. For the C-EIA test performance a reagent for specific antibody detection was prepared using sheep's red blood cells (SRBC) in a concentration of 0.25% sensitized with crude saline extract antigen (SEA) obtained from Cysticercus cellulosae. The optimum concentration of SEA for SRBC sensitization was 40ug/ml. The yield of SEA was 0.lug protein/cavity. When 58 CSF samples from patients with neurocysticercosis were analysed the sensitivity of the test was found to be 84.5% and the confidence limit of 95% probability (CL 95%) ranged from 75% to 94%. The specificity was 95.3% (CL 95% from 90.7% to 99.9%) when CSF samples from the control group were analysed. The C-EIA test was shown to be efficient for neurocysticercosis diagnosis and important for public health laboratories, because of its low cost, high reagent yield and ease of use.