Qualidade microbiológica de leite humano obtido em banco de leite

Detalhes bibliográficos
Autor(a) principal: Serafini, Álvaro B
Data de Publicação: 2003
Outros Autores: André, Maria Cláudia D P B, Rodrigues, Márcia A V, Kipnis, André, Carvalho, Cynthia O, Campos, Maria Raquel H, Monteiro, Érica C, Martins, Fábia, Jubé, Thiago F N
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista de Saúde Pública
Texto Completo: https://www.revistas.usp.br/rsp/article/view/31661
Resumo: OBJECTIVE: The objectives of the present study were to determine the prevalence of potentially pathogenic microorganisms that indicate the hygienic and sanitary conditions of human milk samples collected at a Human Milk Bank. METHODS: Three hundred and thirty eight (338) samples of human milk collected from a milk bank in a maternity in the municipality of Goiânia, in the state of Goias, Brazil were submitted to microbiological analysis. The latter were plated on McConkey agar according to the type of bacteria. Among the total number of samples collected, 194 consisted of raw milk and the remaining 144 were pasteurized milk. RESULTS: The presence of Staphylococcus spp., Streptococcus spp., yeasts and molds, and Enterobacteriaceae was verified in the raw milk samples. Staphylococcus aureus were isolated in 10 (5.2%) samples, Staphylococcus epidermidis in 28 (14.4%) samples, Streptococcus spp. in three (1.6%) samples, yeasts and molds in 43 (22.2%) and Enterobacteriaceae in 49 (25.3%) samples. In a hundred and forty four (144) samples which underwent thermal treatment Staphylococcus aureus was detected in five (3.5%) samples, Staphylococcus epidermidis in 15 (10.4%), Staphylococcus lugdenensis in two (1.4%), Streptococcus spp. in four (2.8%), yeasts and molds in 37 (25.7%), and Enterobacteriaceae in nine (6.3%). CONCLUSIONS: Analysis indicated a high degree of contamination in raw human milk, and as for the pasteurized milk, despite elimination of the great majority of potentially pathogenic microorganisms, the percentage of yeasts and molds was higher than in raw milk, demonstrating that a lower degree of initial contamination would be necessary for pasteurization to be an efficient means of microbiological control.
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spelling Qualidade microbiológica de leite humano obtido em banco de leite Microbiological quality of human milk from a Brazilian milk bank Bancos de leiteLeite humanoControle de qualidadeMilk banksMilkhumanQuality control OBJECTIVE: The objectives of the present study were to determine the prevalence of potentially pathogenic microorganisms that indicate the hygienic and sanitary conditions of human milk samples collected at a Human Milk Bank. METHODS: Three hundred and thirty eight (338) samples of human milk collected from a milk bank in a maternity in the municipality of Goiânia, in the state of Goias, Brazil were submitted to microbiological analysis. The latter were plated on McConkey agar according to the type of bacteria. Among the total number of samples collected, 194 consisted of raw milk and the remaining 144 were pasteurized milk. RESULTS: The presence of Staphylococcus spp., Streptococcus spp., yeasts and molds, and Enterobacteriaceae was verified in the raw milk samples. Staphylococcus aureus were isolated in 10 (5.2%) samples, Staphylococcus epidermidis in 28 (14.4%) samples, Streptococcus spp. in three (1.6%) samples, yeasts and molds in 43 (22.2%) and Enterobacteriaceae in 49 (25.3%) samples. In a hundred and forty four (144) samples which underwent thermal treatment Staphylococcus aureus was detected in five (3.5%) samples, Staphylococcus epidermidis in 15 (10.4%), Staphylococcus lugdenensis in two (1.4%), Streptococcus spp. in four (2.8%), yeasts and molds in 37 (25.7%), and Enterobacteriaceae in nine (6.3%). CONCLUSIONS: Analysis indicated a high degree of contamination in raw human milk, and as for the pasteurized milk, despite elimination of the great majority of potentially pathogenic microorganisms, the percentage of yeasts and molds was higher than in raw milk, demonstrating that a lower degree of initial contamination would be necessary for pasteurization to be an efficient means of microbiological control. OBJETIVO: Determinar a prevalência de microrganismos indicadores e potencialmente patogênicos que indicam as condições higiênico-sanitárias das amostras de leite humano ordenhado coletadas em banco de leite. MÉTODOS: Foram realizadas análises microbiológicas de 338 amostras de leite humano ordenhado, sendo 194 de leite cru e 144, pasteurizado, coletadas em banco de leite humano de um hospital materno infantil de Goiânia, GO. As análises microbiológicas foram realizadas com semeadura em ágar Mc Conkey, de acordo com o tipo de bactéria. RESULTADOS: No leite cru, verificou-se a presença de Staphylococcus spp. Streptococcus spp., bolores e leveduras e Enterobacteriaceae. Observou-se que Staphylococcus aureus esteve presente em 10 (5,2%) amostras, Staphylococcus epidermidis em 28 (14,4%), Streptococcus spp. em três (1,6%), bolores e leveduras em 43 (22,2%) e Enterobacteriaceae em 49 (25,3%). Das 144 amostras de leite humano ordenhado pasteurizado, detectaram-se Staphylococcus aureus em cinco (3,5%), Staphylococcus epidermidis em 15 (10,4%), Staphylococcus lugdenensis em duas (1,4%), Streptococcus spp. em quatro (2,8%), bolores e leveduras em 37 (25,7%) e Enterobacteriaceae em nove (6,3%). CONCLUSÕES: Os resultados mostraram um alto grau de contaminação no leite cru. No leite pasteurizado, apesar da eliminação da grande maioria de microrganismos potencialmente patogênicos, a percentagem de bolores e leveduras excedeu a de leite cru, mostrando a necessidade de obtenção de um leite com carga microbiana inicial mais baixa para que a pasteurização seja eficiente no controle microbiológico. Universidade de São Paulo. Faculdade de Saúde Pública2003-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rsp/article/view/3166110.1590/S0034-89102003000600013Revista de Saúde Pública; Vol. 37 No. 6 (2003); 775-779 Revista de Saúde Pública; Vol. 37 Núm. 6 (2003); 775-779 Revista de Saúde Pública; v. 37 n. 6 (2003); 775-779 1518-87870034-8910reponame:Revista de Saúde Públicainstname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/rsp/article/view/31661/33547Copyright (c) 2017 Revista de Saúde Públicainfo:eu-repo/semantics/openAccessSerafini, Álvaro BAndré, Maria Cláudia D P BRodrigues, Márcia A VKipnis, AndréCarvalho, Cynthia OCampos, Maria Raquel HMonteiro, Érica CMartins, FábiaJubé, Thiago F N2012-07-08T15:01:52Zoai:revistas.usp.br:article/31661Revistahttps://www.revistas.usp.br/rsp/indexONGhttps://www.revistas.usp.br/rsp/oairevsp@org.usp.br||revsp1@usp.br1518-87870034-8910opendoar:2012-07-08T15:01:52Revista de Saúde Pública - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Qualidade microbiológica de leite humano obtido em banco de leite
Microbiological quality of human milk from a Brazilian milk bank
title Qualidade microbiológica de leite humano obtido em banco de leite
spellingShingle Qualidade microbiológica de leite humano obtido em banco de leite
Serafini, Álvaro B
Bancos de leite
Leite humano
Controle de qualidade
Milk banks
Milk
human
Quality control
title_short Qualidade microbiológica de leite humano obtido em banco de leite
title_full Qualidade microbiológica de leite humano obtido em banco de leite
title_fullStr Qualidade microbiológica de leite humano obtido em banco de leite
title_full_unstemmed Qualidade microbiológica de leite humano obtido em banco de leite
title_sort Qualidade microbiológica de leite humano obtido em banco de leite
author Serafini, Álvaro B
author_facet Serafini, Álvaro B
André, Maria Cláudia D P B
Rodrigues, Márcia A V
Kipnis, André
Carvalho, Cynthia O
Campos, Maria Raquel H
Monteiro, Érica C
Martins, Fábia
Jubé, Thiago F N
author_role author
author2 André, Maria Cláudia D P B
Rodrigues, Márcia A V
Kipnis, André
Carvalho, Cynthia O
Campos, Maria Raquel H
Monteiro, Érica C
Martins, Fábia
Jubé, Thiago F N
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Serafini, Álvaro B
André, Maria Cláudia D P B
Rodrigues, Márcia A V
Kipnis, André
Carvalho, Cynthia O
Campos, Maria Raquel H
Monteiro, Érica C
Martins, Fábia
Jubé, Thiago F N
dc.subject.por.fl_str_mv Bancos de leite
Leite humano
Controle de qualidade
Milk banks
Milk
human
Quality control
topic Bancos de leite
Leite humano
Controle de qualidade
Milk banks
Milk
human
Quality control
description OBJECTIVE: The objectives of the present study were to determine the prevalence of potentially pathogenic microorganisms that indicate the hygienic and sanitary conditions of human milk samples collected at a Human Milk Bank. METHODS: Three hundred and thirty eight (338) samples of human milk collected from a milk bank in a maternity in the municipality of Goiânia, in the state of Goias, Brazil were submitted to microbiological analysis. The latter were plated on McConkey agar according to the type of bacteria. Among the total number of samples collected, 194 consisted of raw milk and the remaining 144 were pasteurized milk. RESULTS: The presence of Staphylococcus spp., Streptococcus spp., yeasts and molds, and Enterobacteriaceae was verified in the raw milk samples. Staphylococcus aureus were isolated in 10 (5.2%) samples, Staphylococcus epidermidis in 28 (14.4%) samples, Streptococcus spp. in three (1.6%) samples, yeasts and molds in 43 (22.2%) and Enterobacteriaceae in 49 (25.3%) samples. In a hundred and forty four (144) samples which underwent thermal treatment Staphylococcus aureus was detected in five (3.5%) samples, Staphylococcus epidermidis in 15 (10.4%), Staphylococcus lugdenensis in two (1.4%), Streptococcus spp. in four (2.8%), yeasts and molds in 37 (25.7%), and Enterobacteriaceae in nine (6.3%). CONCLUSIONS: Analysis indicated a high degree of contamination in raw human milk, and as for the pasteurized milk, despite elimination of the great majority of potentially pathogenic microorganisms, the percentage of yeasts and molds was higher than in raw milk, demonstrating that a lower degree of initial contamination would be necessary for pasteurization to be an efficient means of microbiological control.
publishDate 2003
dc.date.none.fl_str_mv 2003-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rsp/article/view/31661
10.1590/S0034-89102003000600013
url https://www.revistas.usp.br/rsp/article/view/31661
identifier_str_mv 10.1590/S0034-89102003000600013
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rsp/article/view/31661/33547
dc.rights.driver.fl_str_mv Copyright (c) 2017 Revista de Saúde Pública
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Revista de Saúde Pública
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Saúde Pública
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Saúde Pública
dc.source.none.fl_str_mv Revista de Saúde Pública; Vol. 37 No. 6 (2003); 775-779
Revista de Saúde Pública; Vol. 37 Núm. 6 (2003); 775-779
Revista de Saúde Pública; v. 37 n. 6 (2003); 775-779
1518-8787
0034-8910
reponame:Revista de Saúde Pública
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Revista de Saúde Pública
collection Revista de Saúde Pública
repository.name.fl_str_mv Revista de Saúde Pública - Universidade de São Paulo (USP)
repository.mail.fl_str_mv revsp@org.usp.br||revsp1@usp.br
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