Isolation of potencially pathogenic free-living amoebas in hospital dust

• Autor(a) principal: Silva, Maria Aparecida da
• Data de Publicação: 2003
• Outros Autores: Rosa, João Aristeu da
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista de Saúde Pública
• Texto Completo: https://www.revistas.usp.br/rsp/article/view/31578
• topic: Acanthamoeba^i1^sisolame; Naegleria^i1^sisolame; Hospitais; Amoeba; Poeira^i1^sanál; Infecção hospitalar^i1^spreven; Naegleria; Amebas de vida livre; Acanthamoeba^i2^sisolat; Purification; Naegleria^i2^sisolat; Purification^i2^shospit; Amoeba; Dust^i2^sanaly; Cross infection; Prevention control; Naegleria; Free-living amoebas

OBJECTIVE: To evaluate the occurrence of free-living amoebas of the genera Acanthamoeba and Naegleria is dust samples colleted in two hospitals. METHODS: One-hundred and thirty-two dust samples were collected in two hospitals in Brazil. Hospital collection sites were the following: intensive care unit, operation rooms, nursery, kitchen, emergency and infectious diseases isolation room . The isolation of the amoebas was performed in three culture media: non-nutrient agar inoculated with Escherichia coli, soy agar, and microculture in Giazzi-modified Pavlova's medium. The amoebas were identified according to morphological criteria. RESULTS: Amoebas of the genera Acanthamoeba and Naegleria were found in 45.5% of the samples, of which 41.6% were collected in the university hospital and 50% in the state hospital. Of all, 45.5% were positive for the genera Acanthamoeba and 3.8% for genera Naegleria. CONCLUSIONS: Potentially pathogenic free-living amoebas were seen in all sites of the two hospitals and Acanthamoeba was the most frequently isolated genera.