Isolation of potencially pathogenic free-living amoebas in hospital dust
Autor(a) principal: | |
---|---|
Data de Publicação: | 2003 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista de Saúde Pública |
Texto Completo: | https://www.revistas.usp.br/rsp/article/view/31578 |
Resumo: | OBJECTIVE: To evaluate the occurrence of free-living amoebas of the genera Acanthamoeba and Naegleria is dust samples colleted in two hospitals. METHODS: One-hundred and thirty-two dust samples were collected in two hospitals in Brazil. Hospital collection sites were the following: intensive care unit, operation rooms, nursery, kitchen, emergency and infectious diseases isolation room . The isolation of the amoebas was performed in three culture media: non-nutrient agar inoculated with Escherichia coli, soy agar, and microculture in Giazzi-modified Pavlova's medium. The amoebas were identified according to morphological criteria. RESULTS: Amoebas of the genera Acanthamoeba and Naegleria were found in 45.5% of the samples, of which 41.6% were collected in the university hospital and 50% in the state hospital. Of all, 45.5% were positive for the genera Acanthamoeba and 3.8% for genera Naegleria. CONCLUSIONS: Potentially pathogenic free-living amoebas were seen in all sites of the two hospitals and Acanthamoeba was the most frequently isolated genera. |
id |
USP-23_982fa8f16d150e2e2d9b27ff206f9310 |
---|---|
oai_identifier_str |
oai:revistas.usp.br:article/31578 |
network_acronym_str |
USP-23 |
network_name_str |
Revista de Saúde Pública |
repository_id_str |
|
spelling |
Isolation of potencially pathogenic free-living amoebas in hospital dust Isolamento de amebas de vida livre potencialmente patogênicas em poeira de hospitais Acanthamoeba^i1^sisolameNaegleria^i1^sisolameHospitaisAmoebaPoeira^i1^sanálInfecção hospitalar^i1^sprevenNaegleriaAmebas de vida livreAcanthamoeba^i2^sisolatPurificationNaegleria^i2^sisolatPurification^i2^shospitAmoebaDust^i2^sanalyCross infectionPrevention controlNaegleriaFree-living amoebas OBJECTIVE: To evaluate the occurrence of free-living amoebas of the genera Acanthamoeba and Naegleria is dust samples colleted in two hospitals. METHODS: One-hundred and thirty-two dust samples were collected in two hospitals in Brazil. Hospital collection sites were the following: intensive care unit, operation rooms, nursery, kitchen, emergency and infectious diseases isolation room . The isolation of the amoebas was performed in three culture media: non-nutrient agar inoculated with Escherichia coli, soy agar, and microculture in Giazzi-modified Pavlova's medium. The amoebas were identified according to morphological criteria. RESULTS: Amoebas of the genera Acanthamoeba and Naegleria were found in 45.5% of the samples, of which 41.6% were collected in the university hospital and 50% in the state hospital. Of all, 45.5% were positive for the genera Acanthamoeba and 3.8% for genera Naegleria. CONCLUSIONS: Potentially pathogenic free-living amoebas were seen in all sites of the two hospitals and Acanthamoeba was the most frequently isolated genera. OBJETIVO: Observar a ocorrência de amebas de vida livre dos gêneros Acanthamoeba e Naegleria em amostras de poeira coletadas em hospitais. MÉTODOS: Foram coletadas 132 amostras de poeira em dois hospitais do município de Presidente Prudente, São Paulo. Os locais da coleta foram: Unidade de Terapia Intensiva, Centro Cirúrgico, Isolamento de Moléstias Infecciosas, Berçário, Emergência e Cozinha. As amostras foram semeadas em três meios de cultura: meio de ágar não nutriente com Escherichia coli, meio de ágar infusão de soja e microcultivo em meio de Pavlova modificado por Giazzi. As amebas isoladas foram identificadas segundo critérios morfológicos. RESULTADOS: O índice geral de positividade para amebas de vida livre, potencialmente patogênicas, dos gêneros Acanthamoeba e Naegleria, foi de 45,5%, sendo positivas 41,6% das amostras de poeira coletadas no hospital universitário e 50% no hospital estadual. Obtiveram-se 45,5% de positividade do gênero Acanthamoeba e 3,8% para amebas do gênero Naegleria. CONCLUSÕES: As amebas de vida livre, potencialmente patogênicas, estavam presentes em todos os ambientes estudados dos dois hospitais, sendo que as espécies do gênero Acanthamoeba foram as isoladas com maior freqüência. Universidade de São Paulo. Faculdade de Saúde Pública2003-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rsp/article/view/3157810.1590/S0034-89102003000200013Revista de Saúde Pública; Vol. 37 No. 2 (2003); 242-246 Revista de Saúde Pública; Vol. 37 Núm. 2 (2003); 242-246 Revista de Saúde Pública; v. 37 n. 2 (2003); 242-246 1518-87870034-8910reponame:Revista de Saúde Públicainstname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/rsp/article/view/31578/33463Copyright (c) 2017 Revista de Saúde Públicainfo:eu-repo/semantics/openAccessSilva, Maria Aparecida daRosa, João Aristeu da2012-07-08T14:53:15Zoai:revistas.usp.br:article/31578Revistahttps://www.revistas.usp.br/rsp/indexONGhttps://www.revistas.usp.br/rsp/oairevsp@org.usp.br||revsp1@usp.br1518-87870034-8910opendoar:2012-07-08T14:53:15Revista de Saúde Pública - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Isolation of potencially pathogenic free-living amoebas in hospital dust Isolamento de amebas de vida livre potencialmente patogênicas em poeira de hospitais |
title |
Isolation of potencially pathogenic free-living amoebas in hospital dust |
spellingShingle |
Isolation of potencially pathogenic free-living amoebas in hospital dust Silva, Maria Aparecida da Acanthamoeba^i1^sisolame Naegleria^i1^sisolame Hospitais Amoeba Poeira^i1^sanál Infecção hospitalar^i1^spreven Naegleria Amebas de vida livre Acanthamoeba^i2^sisolat Purification Naegleria^i2^sisolat Purification^i2^shospit Amoeba Dust^i2^sanaly Cross infection Prevention control Naegleria Free-living amoebas |
title_short |
Isolation of potencially pathogenic free-living amoebas in hospital dust |
title_full |
Isolation of potencially pathogenic free-living amoebas in hospital dust |
title_fullStr |
Isolation of potencially pathogenic free-living amoebas in hospital dust |
title_full_unstemmed |
Isolation of potencially pathogenic free-living amoebas in hospital dust |
title_sort |
Isolation of potencially pathogenic free-living amoebas in hospital dust |
author |
Silva, Maria Aparecida da |
author_facet |
Silva, Maria Aparecida da Rosa, João Aristeu da |
author_role |
author |
author2 |
Rosa, João Aristeu da |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Silva, Maria Aparecida da Rosa, João Aristeu da |
dc.subject.por.fl_str_mv |
Acanthamoeba^i1^sisolame Naegleria^i1^sisolame Hospitais Amoeba Poeira^i1^sanál Infecção hospitalar^i1^spreven Naegleria Amebas de vida livre Acanthamoeba^i2^sisolat Purification Naegleria^i2^sisolat Purification^i2^shospit Amoeba Dust^i2^sanaly Cross infection Prevention control Naegleria Free-living amoebas |
topic |
Acanthamoeba^i1^sisolame Naegleria^i1^sisolame Hospitais Amoeba Poeira^i1^sanál Infecção hospitalar^i1^spreven Naegleria Amebas de vida livre Acanthamoeba^i2^sisolat Purification Naegleria^i2^sisolat Purification^i2^shospit Amoeba Dust^i2^sanaly Cross infection Prevention control Naegleria Free-living amoebas |
description |
OBJECTIVE: To evaluate the occurrence of free-living amoebas of the genera Acanthamoeba and Naegleria is dust samples colleted in two hospitals. METHODS: One-hundred and thirty-two dust samples were collected in two hospitals in Brazil. Hospital collection sites were the following: intensive care unit, operation rooms, nursery, kitchen, emergency and infectious diseases isolation room . The isolation of the amoebas was performed in three culture media: non-nutrient agar inoculated with Escherichia coli, soy agar, and microculture in Giazzi-modified Pavlova's medium. The amoebas were identified according to morphological criteria. RESULTS: Amoebas of the genera Acanthamoeba and Naegleria were found in 45.5% of the samples, of which 41.6% were collected in the university hospital and 50% in the state hospital. Of all, 45.5% were positive for the genera Acanthamoeba and 3.8% for genera Naegleria. CONCLUSIONS: Potentially pathogenic free-living amoebas were seen in all sites of the two hospitals and Acanthamoeba was the most frequently isolated genera. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rsp/article/view/31578 10.1590/S0034-89102003000200013 |
url |
https://www.revistas.usp.br/rsp/article/view/31578 |
identifier_str_mv |
10.1590/S0034-89102003000200013 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rsp/article/view/31578/33463 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Revista de Saúde Pública info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Revista de Saúde Pública |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Saúde Pública |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Saúde Pública |
dc.source.none.fl_str_mv |
Revista de Saúde Pública; Vol. 37 No. 2 (2003); 242-246 Revista de Saúde Pública; Vol. 37 Núm. 2 (2003); 242-246 Revista de Saúde Pública; v. 37 n. 2 (2003); 242-246 1518-8787 0034-8910 reponame:Revista de Saúde Pública instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Revista de Saúde Pública |
collection |
Revista de Saúde Pública |
repository.name.fl_str_mv |
Revista de Saúde Pública - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
revsp@org.usp.br||revsp1@usp.br |
_version_ |
1800221781244510208 |