Antischistosomal activity of artemether in experimental Schistosomiasis mansoni

Detalhes bibliográficos
Autor(a) principal: Lescano, Susana Zevallos
Data de Publicação: 2004
Outros Autores: Chieffi, Pedro Paulo, Canhassi, Rosa Regina, Boulos, Marcos, Amato Neto, Vicente
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista de Saúde Pública
DOI: 10.1590/S0034-89102004000100010
Texto Completo: https://www.revistas.usp.br/rsp/article/view/31681
Resumo: OBJECTIVE: To evaluate the effect of intramuscular injection of artemether in mice experimentally infected with Schistosoma mansoni , at the time of infection, during schistosomula maturation and after the beginning of egg-laying. METHODS: Eighty adult females Balb/c mice were divided into 8 groups with 10 animals each. Seven groups were infected with S. mansoni using 60 cercariae for each animal, inoculated subcutaneously, and the remaining group was maintained without infection. Among the seven infected groups, six were treated with artemether, according to the following schedule: three groups received doses of 100 mg/kg on days 0, 20 or 60 after inoculation of the cercariae; the other three received 50 mg/kg of artemether, also on days 0, 20 or 60. At the end of the 9th, 10th and 11th weeks after infection all the mice infected with S. mansoni were submitted to fecal examination using the Kato-Katz technique. On the 80th day of the experiment, the surviving animals were sacrificed and submitted to perfusion of the portal system in order to recover the worms. Body, liver and spleen weights of each animal were determined at that time. RESULTS: A reduction in egg-laying and the number of worms recovered was observed in mice treated with artemether (50 or 100 mg/kg) on the 20th day after infection. The decrease in the number of worms was more notable among S. mansoni females. A significant decrease in liver and spleen weights was also seen on the 20th day among animals treated with 50 or 100 mg/kg of artemether and also among those that received the drug at a dose of 50 mg/kg 60 days after infection. CONCLUSIONS: Evidence of the antischistosomal activity of artemether was shown, even at a dose of 50 mg/kg, when the drug was administered during the schistosomula maturation period in the portal system of the vertebrate host.
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spelling Antischistosomal activity of artemether in experimental Schistosomiasis mansoni Atividade antiparasitária do artemether na esquistossomose mansônica experimental Schistosoma mansoniArtemisininas^i1^suso terapêutEsquistossomose mansoni^i1^squimioteraExperimentaçäo animalArtemetherSchistosoma mansoniArtemisinins^i2^stherapeuticSchistosomiasis mansoni^i2^sdrug therAnimal experimentationArtemether OBJECTIVE: To evaluate the effect of intramuscular injection of artemether in mice experimentally infected with Schistosoma mansoni , at the time of infection, during schistosomula maturation and after the beginning of egg-laying. METHODS: Eighty adult females Balb/c mice were divided into 8 groups with 10 animals each. Seven groups were infected with S. mansoni using 60 cercariae for each animal, inoculated subcutaneously, and the remaining group was maintained without infection. Among the seven infected groups, six were treated with artemether, according to the following schedule: three groups received doses of 100 mg/kg on days 0, 20 or 60 after inoculation of the cercariae; the other three received 50 mg/kg of artemether, also on days 0, 20 or 60. At the end of the 9th, 10th and 11th weeks after infection all the mice infected with S. mansoni were submitted to fecal examination using the Kato-Katz technique. On the 80th day of the experiment, the surviving animals were sacrificed and submitted to perfusion of the portal system in order to recover the worms. Body, liver and spleen weights of each animal were determined at that time. RESULTS: A reduction in egg-laying and the number of worms recovered was observed in mice treated with artemether (50 or 100 mg/kg) on the 20th day after infection. The decrease in the number of worms was more notable among S. mansoni females. A significant decrease in liver and spleen weights was also seen on the 20th day among animals treated with 50 or 100 mg/kg of artemether and also among those that received the drug at a dose of 50 mg/kg 60 days after infection. CONCLUSIONS: Evidence of the antischistosomal activity of artemether was shown, even at a dose of 50 mg/kg, when the drug was administered during the schistosomula maturation period in the portal system of the vertebrate host. OBJETIVO: Avaliar o efeito da administração intramuscular de artemether a camundongos infectados experimentalmente por Schistosoma mansoni no momento da infecção, durante a maturação dos esquistossômulos e após iniciada a oviposição. MÉTODOS: Oitenta camundongos Balb/c, fêmeas adultas, foram divididos em oito grupos com 10 animais cada. Sete grupos foram infectados por S. mansoni empregando-se 60 cercárias para cada animal, inoculadas por via subcutânea; o grupo restante foi mantido sem infecção. Entre os sete grupos infectados, seis foram tratados com artemether, segundo o seguinte esquema: três grupos receberam dose correspondente a 100 mg/kg no dia 0, 20 ou 60 após inoculação das cercárias; os demais receberam 50 mg/kg de artemether, no mesmo período que os lotes anteriores. Da 9ª, 10ª e 11ª semanas após infecção os camundongos infectados por S. mansoni foram submetidos a exames de fezes pela técnica de Kato-Katz. No 80º dia do experimento, os animais sobreviventes foram sacrificados e submetidos à perfusão do sistema porta para recuperação de vermes. Determinaram-se, nessa ocasião, os pesos corporal, hepático e esplênico de cada animal. RESULTADOS: Observou-se queda na oviposição e no número de vermes recuperados entre os camundongos tratados com artemether (50 ou 100 mg/kg) no 20º dia após infecção. A diminuição do número de vermes foi mais expressiva no caso de fêmeas de S. mansoni. Verificou-se, ainda, diminuição significativa nos pesos hepático e esplênico entre os animais tratados com 50 e 100 mg/kg de artemether no 20º dia e também entre os que receberam a droga na dose de 50 mg/kg 60 dias após infecção. CONCLUSÕES: Ficou evidenciada a atividade anti-Schistosoma do artemether, mesmo ao se empregar dose correspondente a 50 mg/kg, quando a droga foi administrada durante o período de maturação dos esquistossômulos no sistema porta do hospedeiro vertebrado. Universidade de São Paulo. Faculdade de Saúde Pública2004-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rsp/article/view/3168110.1590/S0034-89102004000100010Revista de Saúde Pública; Vol. 38 No. 1 (2004); 71-75 Revista de Saúde Pública; Vol. 38 Núm. 1 (2004); 71-75 Revista de Saúde Pública; v. 38 n. 1 (2004); 71-75 1518-87870034-8910reponame:Revista de Saúde Públicainstname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/rsp/article/view/31681/33567Copyright (c) 2017 Revista de Saúde Públicainfo:eu-repo/semantics/openAccessLescano, Susana ZevallosChieffi, Pedro PauloCanhassi, Rosa ReginaBoulos, MarcosAmato Neto, Vicente2012-07-08T21:58:41Zoai:revistas.usp.br:article/31681Revistahttps://www.revistas.usp.br/rsp/indexONGhttps://www.revistas.usp.br/rsp/oairevsp@org.usp.br||revsp1@usp.br1518-87870034-8910opendoar:2012-07-08T21:58:41Revista de Saúde Pública - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Antischistosomal activity of artemether in experimental Schistosomiasis mansoni
Atividade antiparasitária do artemether na esquistossomose mansônica experimental
title Antischistosomal activity of artemether in experimental Schistosomiasis mansoni
spellingShingle Antischistosomal activity of artemether in experimental Schistosomiasis mansoni
Antischistosomal activity of artemether in experimental Schistosomiasis mansoni
Lescano, Susana Zevallos
Schistosoma mansoni
Artemisininas^i1^suso terapêut
Esquistossomose mansoni^i1^squimiotera
Experimentaçäo animal
Artemether
Schistosoma mansoni
Artemisinins^i2^stherapeutic
Schistosomiasis mansoni^i2^sdrug ther
Animal experimentation
Artemether
Lescano, Susana Zevallos
Schistosoma mansoni
Artemisininas^i1^suso terapêut
Esquistossomose mansoni^i1^squimiotera
Experimentaçäo animal
Artemether
Schistosoma mansoni
Artemisinins^i2^stherapeutic
Schistosomiasis mansoni^i2^sdrug ther
Animal experimentation
Artemether
title_short Antischistosomal activity of artemether in experimental Schistosomiasis mansoni
title_full Antischistosomal activity of artemether in experimental Schistosomiasis mansoni
title_fullStr Antischistosomal activity of artemether in experimental Schistosomiasis mansoni
Antischistosomal activity of artemether in experimental Schistosomiasis mansoni
title_full_unstemmed Antischistosomal activity of artemether in experimental Schistosomiasis mansoni
Antischistosomal activity of artemether in experimental Schistosomiasis mansoni
title_sort Antischistosomal activity of artemether in experimental Schistosomiasis mansoni
author Lescano, Susana Zevallos
author_facet Lescano, Susana Zevallos
Lescano, Susana Zevallos
Chieffi, Pedro Paulo
Canhassi, Rosa Regina
Boulos, Marcos
Amato Neto, Vicente
Chieffi, Pedro Paulo
Canhassi, Rosa Regina
Boulos, Marcos
Amato Neto, Vicente
author_role author
author2 Chieffi, Pedro Paulo
Canhassi, Rosa Regina
Boulos, Marcos
Amato Neto, Vicente
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Lescano, Susana Zevallos
Chieffi, Pedro Paulo
Canhassi, Rosa Regina
Boulos, Marcos
Amato Neto, Vicente
dc.subject.por.fl_str_mv Schistosoma mansoni
Artemisininas^i1^suso terapêut
Esquistossomose mansoni^i1^squimiotera
Experimentaçäo animal
Artemether
Schistosoma mansoni
Artemisinins^i2^stherapeutic
Schistosomiasis mansoni^i2^sdrug ther
Animal experimentation
Artemether
topic Schistosoma mansoni
Artemisininas^i1^suso terapêut
Esquistossomose mansoni^i1^squimiotera
Experimentaçäo animal
Artemether
Schistosoma mansoni
Artemisinins^i2^stherapeutic
Schistosomiasis mansoni^i2^sdrug ther
Animal experimentation
Artemether
description OBJECTIVE: To evaluate the effect of intramuscular injection of artemether in mice experimentally infected with Schistosoma mansoni , at the time of infection, during schistosomula maturation and after the beginning of egg-laying. METHODS: Eighty adult females Balb/c mice were divided into 8 groups with 10 animals each. Seven groups were infected with S. mansoni using 60 cercariae for each animal, inoculated subcutaneously, and the remaining group was maintained without infection. Among the seven infected groups, six were treated with artemether, according to the following schedule: three groups received doses of 100 mg/kg on days 0, 20 or 60 after inoculation of the cercariae; the other three received 50 mg/kg of artemether, also on days 0, 20 or 60. At the end of the 9th, 10th and 11th weeks after infection all the mice infected with S. mansoni were submitted to fecal examination using the Kato-Katz technique. On the 80th day of the experiment, the surviving animals were sacrificed and submitted to perfusion of the portal system in order to recover the worms. Body, liver and spleen weights of each animal were determined at that time. RESULTS: A reduction in egg-laying and the number of worms recovered was observed in mice treated with artemether (50 or 100 mg/kg) on the 20th day after infection. The decrease in the number of worms was more notable among S. mansoni females. A significant decrease in liver and spleen weights was also seen on the 20th day among animals treated with 50 or 100 mg/kg of artemether and also among those that received the drug at a dose of 50 mg/kg 60 days after infection. CONCLUSIONS: Evidence of the antischistosomal activity of artemether was shown, even at a dose of 50 mg/kg, when the drug was administered during the schistosomula maturation period in the portal system of the vertebrate host.
publishDate 2004
dc.date.none.fl_str_mv 2004-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rsp/article/view/31681
10.1590/S0034-89102004000100010
url https://www.revistas.usp.br/rsp/article/view/31681
identifier_str_mv 10.1590/S0034-89102004000100010
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rsp/article/view/31681/33567
dc.rights.driver.fl_str_mv Copyright (c) 2017 Revista de Saúde Pública
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Revista de Saúde Pública
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Saúde Pública
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Saúde Pública
dc.source.none.fl_str_mv Revista de Saúde Pública; Vol. 38 No. 1 (2004); 71-75
Revista de Saúde Pública; Vol. 38 Núm. 1 (2004); 71-75
Revista de Saúde Pública; v. 38 n. 1 (2004); 71-75
1518-8787
0034-8910
reponame:Revista de Saúde Pública
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Revista de Saúde Pública
collection Revista de Saúde Pública
repository.name.fl_str_mv Revista de Saúde Pública - Universidade de São Paulo (USP)
repository.mail.fl_str_mv revsp@org.usp.br||revsp1@usp.br
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dc.identifier.doi.none.fl_str_mv 10.1590/S0034-89102004000100010