Delayed-type skin reaction to tuberculin and the cellular and humoral immune response "in vitro" in patients with pulmonary tuberculosis
Autor(a) principal: | |
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Data de Publicação: | 1983 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista de Saúde Pública |
Texto Completo: | https://www.revistas.usp.br/rsp/article/view/23156 |
Resumo: | This paper presents the results of delayed-type skin reaction to tuberculin and the cellular and humoral immune responses "in vitro" in 50 patients with active pulmonary tuberculosis admitted to the "Parque Hospitalar do Mandaqui", São Paulo, Brazil, in the period from May to August 1980, matched for sex and ranging in age from 20 to 80 years old. In order to study the delayed-type skin reaction to tuberculin, the PPD, Rt-23, 2 TU (Purified protein derivative) was used and the result obtained was of 14.0% of nonreactors, 12.0% of weak reactors and 74.0% of strong reactors. The "in vitro" study of cellular and humoral immune responses was carried out by determining the number of T and B lymphocytes, the lymphocyte transformation, the liberation of the leucocyte inhibitor factor (LIF) and by the passive hemaglutination test. The results showed the importance of calculation of the absolute number of T and B lymphocytes. The culture of lymphocytes and the LIF experiment were able to detect the lymphocyte sensitivity to PPD even in the nonreactor patients and the passive hemaglutination test showed the presence of specific antibodies in the population studied demonstrated by the highest titers when compared with normal subjects, independently of the skin reactivity to tuberculin. |
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Delayed-type skin reaction to tuberculin and the cellular and humoral immune response "in vitro" in patients with pulmonary tuberculosis Reatividade tuberculínica e resposta imunológica celular e humoral "in vitro" em doentes com tuberculose pulmonar Tuberculose pulmonarTeste tuberculínicoTuberculosispulmonaryTuberculin test This paper presents the results of delayed-type skin reaction to tuberculin and the cellular and humoral immune responses "in vitro" in 50 patients with active pulmonary tuberculosis admitted to the "Parque Hospitalar do Mandaqui", São Paulo, Brazil, in the period from May to August 1980, matched for sex and ranging in age from 20 to 80 years old. In order to study the delayed-type skin reaction to tuberculin, the PPD, Rt-23, 2 TU (Purified protein derivative) was used and the result obtained was of 14.0% of nonreactors, 12.0% of weak reactors and 74.0% of strong reactors. The "in vitro" study of cellular and humoral immune responses was carried out by determining the number of T and B lymphocytes, the lymphocyte transformation, the liberation of the leucocyte inhibitor factor (LIF) and by the passive hemaglutination test. The results showed the importance of calculation of the absolute number of T and B lymphocytes. The culture of lymphocytes and the LIF experiment were able to detect the lymphocyte sensitivity to PPD even in the nonreactor patients and the passive hemaglutination test showed the presence of specific antibodies in the population studied demonstrated by the highest titers when compared with normal subjects, independently of the skin reactivity to tuberculin. Foi estudada a reatividade tuberculínica e a resposta imunológica celular e humoral "in vitro", em 50 doentes de ambos os sexos, de 20 a 80 anos de idade, com tuberculose pulmonar ativa, internados no Parque Hospitalar do Mandaqui da Secretaria de Estado da Saúde, São Paulo (Brasil), no período de maio a agosto de 1980. Para o estudo da reatividade tuberculínica foi utilizado o PPD, Rt-23, 2 UT, tendo havido 14,0% de não-reatores, 12,0% de reatores fracos e 74,0% de reatores fortes. O estudo da imunidade celular e humoral "in vitro" foi realizado pela quantificação de linfócitos T e B, transformação blástica de linfócitos, liberação do fator inibidor da migração de leucócitos (LIF) e reação de hemaglutinação passiva. Os resultados mostraram a validade do cálculo do número absoluto dos linfócitos T e B. A cultura de linfócitos e a técnica do LIF, foram capazes de detectar a sensibilização dos linfócitos ao PPD, mesmo nos doentes não reatores, e a reação de hemaglutinação passiva revelou a presença de anticorpos específicos na população estudada em títulos superiores aos encontrados em pessoas normais, independentemente da reatividade tuberculínica. Universidade de São Paulo. Faculdade de Saúde Pública1983-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rsp/article/view/2315610.1590/S0034-89101983000200004Revista de Saúde Pública; Vol. 17 No. 2 (1983); 94-111 Revista de Saúde Pública; Vol. 17 Núm. 2 (1983); 94-111 Revista de Saúde Pública; v. 17 n. 2 (1983); 94-111 1518-87870034-8910reponame:Revista de Saúde Públicainstname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/rsp/article/view/23156/25182Copyright (c) 2017 Revista de Saúde Públicainfo:eu-repo/semantics/openAccessAraújo, Adele Caterino deTakeda, Augusta KiyomiHerrero, Cleonice BegoFreitas, Irene Walter deNakandakare, Ivete KazueGuedes, Eloísa AparecidaBrólio, Roberto2012-05-28T15:06:17Zoai:revistas.usp.br:article/23156Revistahttps://www.revistas.usp.br/rsp/indexONGhttps://www.revistas.usp.br/rsp/oairevsp@org.usp.br||revsp1@usp.br1518-87870034-8910opendoar:2012-05-28T15:06:17Revista de Saúde Pública - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Delayed-type skin reaction to tuberculin and the cellular and humoral immune response "in vitro" in patients with pulmonary tuberculosis Reatividade tuberculínica e resposta imunológica celular e humoral "in vitro" em doentes com tuberculose pulmonar |
title |
Delayed-type skin reaction to tuberculin and the cellular and humoral immune response "in vitro" in patients with pulmonary tuberculosis |
spellingShingle |
Delayed-type skin reaction to tuberculin and the cellular and humoral immune response "in vitro" in patients with pulmonary tuberculosis Araújo, Adele Caterino de Tuberculose pulmonar Teste tuberculínico Tuberculosis pulmonary Tuberculin test |
title_short |
Delayed-type skin reaction to tuberculin and the cellular and humoral immune response "in vitro" in patients with pulmonary tuberculosis |
title_full |
Delayed-type skin reaction to tuberculin and the cellular and humoral immune response "in vitro" in patients with pulmonary tuberculosis |
title_fullStr |
Delayed-type skin reaction to tuberculin and the cellular and humoral immune response "in vitro" in patients with pulmonary tuberculosis |
title_full_unstemmed |
Delayed-type skin reaction to tuberculin and the cellular and humoral immune response "in vitro" in patients with pulmonary tuberculosis |
title_sort |
Delayed-type skin reaction to tuberculin and the cellular and humoral immune response "in vitro" in patients with pulmonary tuberculosis |
author |
Araújo, Adele Caterino de |
author_facet |
Araújo, Adele Caterino de Takeda, Augusta Kiyomi Herrero, Cleonice Bego Freitas, Irene Walter de Nakandakare, Ivete Kazue Guedes, Eloísa Aparecida Brólio, Roberto |
author_role |
author |
author2 |
Takeda, Augusta Kiyomi Herrero, Cleonice Bego Freitas, Irene Walter de Nakandakare, Ivete Kazue Guedes, Eloísa Aparecida Brólio, Roberto |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Araújo, Adele Caterino de Takeda, Augusta Kiyomi Herrero, Cleonice Bego Freitas, Irene Walter de Nakandakare, Ivete Kazue Guedes, Eloísa Aparecida Brólio, Roberto |
dc.subject.por.fl_str_mv |
Tuberculose pulmonar Teste tuberculínico Tuberculosis pulmonary Tuberculin test |
topic |
Tuberculose pulmonar Teste tuberculínico Tuberculosis pulmonary Tuberculin test |
description |
This paper presents the results of delayed-type skin reaction to tuberculin and the cellular and humoral immune responses "in vitro" in 50 patients with active pulmonary tuberculosis admitted to the "Parque Hospitalar do Mandaqui", São Paulo, Brazil, in the period from May to August 1980, matched for sex and ranging in age from 20 to 80 years old. In order to study the delayed-type skin reaction to tuberculin, the PPD, Rt-23, 2 TU (Purified protein derivative) was used and the result obtained was of 14.0% of nonreactors, 12.0% of weak reactors and 74.0% of strong reactors. The "in vitro" study of cellular and humoral immune responses was carried out by determining the number of T and B lymphocytes, the lymphocyte transformation, the liberation of the leucocyte inhibitor factor (LIF) and by the passive hemaglutination test. The results showed the importance of calculation of the absolute number of T and B lymphocytes. The culture of lymphocytes and the LIF experiment were able to detect the lymphocyte sensitivity to PPD even in the nonreactor patients and the passive hemaglutination test showed the presence of specific antibodies in the population studied demonstrated by the highest titers when compared with normal subjects, independently of the skin reactivity to tuberculin. |
publishDate |
1983 |
dc.date.none.fl_str_mv |
1983-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rsp/article/view/23156 10.1590/S0034-89101983000200004 |
url |
https://www.revistas.usp.br/rsp/article/view/23156 |
identifier_str_mv |
10.1590/S0034-89101983000200004 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rsp/article/view/23156/25182 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Revista de Saúde Pública info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Revista de Saúde Pública |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Saúde Pública |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Saúde Pública |
dc.source.none.fl_str_mv |
Revista de Saúde Pública; Vol. 17 No. 2 (1983); 94-111 Revista de Saúde Pública; Vol. 17 Núm. 2 (1983); 94-111 Revista de Saúde Pública; v. 17 n. 2 (1983); 94-111 1518-8787 0034-8910 reponame:Revista de Saúde Pública instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Revista de Saúde Pública |
collection |
Revista de Saúde Pública |
repository.name.fl_str_mv |
Revista de Saúde Pública - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
revsp@org.usp.br||revsp1@usp.br |
_version_ |
1800221771709808640 |