Interaction study between vancomycin and liposomes containing natural compounds against methicillin-resistant Staphylococcus aureus clinical isolates

Detalhes bibliográficos
Autor(a) principal: Cavalcanti, Isabella Macário Ferro
Data de Publicação: 2018
Outros Autores: Menezes, Talita Gomes Calaça, Campos, Luís André de Almeida, Ferraz, Milena Sales, Maciel, Maria Amélia Vieira, Caetano, Maria Nelly Psiotano, Santos-Magalhães, Nereide Stela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Pharmaceutical Sciences
Texto Completo: https://www.revistas.usp.br/bjps/article/view/153775
Resumo: The treatment of infections caused by resistant microorganisms is limited, and vancomycin (VAN) treatment failures for methicillin-resistant Staphylococcus aureus (MRSA) bacteremia are not uncommon, even when MRSA clinical isolates are susceptible to VAN. Thus, this study proposed the association of VAN with usnic acid and β-lapachone encapsulated into liposomes as a novel therapeutic option for infections caused by MRSA. Liposomes containing β-lap (β-lap-lipo) or usnic acid (UA-lipo) were prepared by the thin lipid film hydration method followed by sonication. Antimicrobial activity against MRSA clinical isolates was investigated by the microdilution method according to the Clinical and Laboratory Standards Institute (CLSI). The interaction studies were carried out using the checkerboard method and epsilometer test (Etest). The interaction between VAN and β-lap or β-lap-lipo was synergistic (FICI = 0.453 and FICI = 0.358, respectively). An additive interaction between VAN and UA (FICI = 0.515) was found. UA-lipo resulted in synergism with VAN (FICI = 0.276). The Etest reproduced the results obtained by the checkerboard method for approximately 82% of the analysis. Thus, the present study demonstrated that VAN in combination with UA-lipo, β-lap or β-lap-lipo synergistically enhanced antibacterial activity against MRSA.
id USP-31_1a659abc84d831f7402a50350ad6edf8
oai_identifier_str oai:revistas.usp.br:article/153775
network_acronym_str USP-31
network_name_str Brazilian Journal of Pharmaceutical Sciences
repository_id_str
spelling Interaction study between vancomycin and liposomes containing natural compounds against methicillin-resistant Staphylococcus aureus clinical isolatesβ-lapachoneUsnic acidLiposomesMethicillin-resistant Staphylococcus aureus (MRSA)SynergismThe treatment of infections caused by resistant microorganisms is limited, and vancomycin (VAN) treatment failures for methicillin-resistant Staphylococcus aureus (MRSA) bacteremia are not uncommon, even when MRSA clinical isolates are susceptible to VAN. Thus, this study proposed the association of VAN with usnic acid and β-lapachone encapsulated into liposomes as a novel therapeutic option for infections caused by MRSA. Liposomes containing β-lap (β-lap-lipo) or usnic acid (UA-lipo) were prepared by the thin lipid film hydration method followed by sonication. Antimicrobial activity against MRSA clinical isolates was investigated by the microdilution method according to the Clinical and Laboratory Standards Institute (CLSI). The interaction studies were carried out using the checkerboard method and epsilometer test (Etest). The interaction between VAN and β-lap or β-lap-lipo was synergistic (FICI = 0.453 and FICI = 0.358, respectively). An additive interaction between VAN and UA (FICI = 0.515) was found. UA-lipo resulted in synergism with VAN (FICI = 0.276). The Etest reproduced the results obtained by the checkerboard method for approximately 82% of the analysis. Thus, the present study demonstrated that VAN in combination with UA-lipo, β-lap or β-lap-lipo synergistically enhanced antibacterial activity against MRSA.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2018-07-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/15377510.1590/s2175-97902018000200203Brazilian Journal of Pharmaceutical Sciences; Vol. 54 Núm. 2 (2018); e00203Brazilian Journal of Pharmaceutical Sciences; v. 54 n. 2 (2018); e00203Brazilian Journal of Pharmaceutical Sciences; Vol. 54 No. 2 (2018); e002032175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/153775/150166Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)info:eu-repo/semantics/openAccessCavalcanti, Isabella Macário FerroMenezes, Talita Gomes CalaçaCampos, Luís André de AlmeidaFerraz, Milena SalesMaciel, Maria Amélia VieiraCaetano, Maria Nelly PsiotanoSantos-Magalhães, Nereide Stela2019-03-17T13:37:08Zoai:revistas.usp.br:article/153775Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2019-03-17T13:37:08Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Interaction study between vancomycin and liposomes containing natural compounds against methicillin-resistant Staphylococcus aureus clinical isolates
title Interaction study between vancomycin and liposomes containing natural compounds against methicillin-resistant Staphylococcus aureus clinical isolates
spellingShingle Interaction study between vancomycin and liposomes containing natural compounds against methicillin-resistant Staphylococcus aureus clinical isolates
Cavalcanti, Isabella Macário Ferro
β-lapachone
Usnic acid
Liposomes
Methicillin-resistant Staphylococcus aureus (MRSA)
Synergism
title_short Interaction study between vancomycin and liposomes containing natural compounds against methicillin-resistant Staphylococcus aureus clinical isolates
title_full Interaction study between vancomycin and liposomes containing natural compounds against methicillin-resistant Staphylococcus aureus clinical isolates
title_fullStr Interaction study between vancomycin and liposomes containing natural compounds against methicillin-resistant Staphylococcus aureus clinical isolates
title_full_unstemmed Interaction study between vancomycin and liposomes containing natural compounds against methicillin-resistant Staphylococcus aureus clinical isolates
title_sort Interaction study between vancomycin and liposomes containing natural compounds against methicillin-resistant Staphylococcus aureus clinical isolates
author Cavalcanti, Isabella Macário Ferro
author_facet Cavalcanti, Isabella Macário Ferro
Menezes, Talita Gomes Calaça
Campos, Luís André de Almeida
Ferraz, Milena Sales
Maciel, Maria Amélia Vieira
Caetano, Maria Nelly Psiotano
Santos-Magalhães, Nereide Stela
author_role author
author2 Menezes, Talita Gomes Calaça
Campos, Luís André de Almeida
Ferraz, Milena Sales
Maciel, Maria Amélia Vieira
Caetano, Maria Nelly Psiotano
Santos-Magalhães, Nereide Stela
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cavalcanti, Isabella Macário Ferro
Menezes, Talita Gomes Calaça
Campos, Luís André de Almeida
Ferraz, Milena Sales
Maciel, Maria Amélia Vieira
Caetano, Maria Nelly Psiotano
Santos-Magalhães, Nereide Stela
dc.subject.por.fl_str_mv β-lapachone
Usnic acid
Liposomes
Methicillin-resistant Staphylococcus aureus (MRSA)
Synergism
topic β-lapachone
Usnic acid
Liposomes
Methicillin-resistant Staphylococcus aureus (MRSA)
Synergism
description The treatment of infections caused by resistant microorganisms is limited, and vancomycin (VAN) treatment failures for methicillin-resistant Staphylococcus aureus (MRSA) bacteremia are not uncommon, even when MRSA clinical isolates are susceptible to VAN. Thus, this study proposed the association of VAN with usnic acid and β-lapachone encapsulated into liposomes as a novel therapeutic option for infections caused by MRSA. Liposomes containing β-lap (β-lap-lipo) or usnic acid (UA-lipo) were prepared by the thin lipid film hydration method followed by sonication. Antimicrobial activity against MRSA clinical isolates was investigated by the microdilution method according to the Clinical and Laboratory Standards Institute (CLSI). The interaction studies were carried out using the checkerboard method and epsilometer test (Etest). The interaction between VAN and β-lap or β-lap-lipo was synergistic (FICI = 0.453 and FICI = 0.358, respectively). An additive interaction between VAN and UA (FICI = 0.515) was found. UA-lipo resulted in synergism with VAN (FICI = 0.276). The Etest reproduced the results obtained by the checkerboard method for approximately 82% of the analysis. Thus, the present study demonstrated that VAN in combination with UA-lipo, β-lap or β-lap-lipo synergistically enhanced antibacterial activity against MRSA.
publishDate 2018
dc.date.none.fl_str_mv 2018-07-26
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjps/article/view/153775
10.1590/s2175-97902018000200203
url https://www.revistas.usp.br/bjps/article/view/153775
identifier_str_mv 10.1590/s2175-97902018000200203
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjps/article/view/153775/150166
dc.rights.driver.fl_str_mv Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
dc.source.none.fl_str_mv Brazilian Journal of Pharmaceutical Sciences; Vol. 54 Núm. 2 (2018); e00203
Brazilian Journal of Pharmaceutical Sciences; v. 54 n. 2 (2018); e00203
Brazilian Journal of Pharmaceutical Sciences; Vol. 54 No. 2 (2018); e00203
2175-9790
1984-8250
reponame:Brazilian Journal of Pharmaceutical Sciences
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Pharmaceutical Sciences
collection Brazilian Journal of Pharmaceutical Sciences
repository.name.fl_str_mv Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)
repository.mail.fl_str_mv bjps@usp.br||elizabeth.igne@gmail.com
_version_ 1821325162145906688