Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds

• Autor(a) principal: Campos Andrade, João Paulo
• Data de Publicação: 2022
• Outros Autores: Alvarenga Alves, Osvaine Junior, Costa, Marian Caroline, Melleiro Gimenez, Valéria Maria, Silva Squarisi, Iara, Diniz Nicolella, Heloiza, Cintra Pagotti, Mariana, Crispim Tavares, Denise, Cunha, Wilson Roberto, Andrade e Silva, Márcio Luís, Januario, Ana Helena, Guidi Magalhães, Lizandra, Mendonça Pauletti, Patrícia
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Pharmaceutical Sciences
• Texto Completo: https://www.revistas.usp.br/bjps/article/view/204952
• topic: Cytotoxicity; Flavonol; HPLC-HRMS-DAD; Iridoid; Leishmanicidal

Adenocalymma axillarum (K.Schum.) L.G. Lohmann is a liana belonging to the family Bignoniaceae. In traditional medicine, the genus Adenocalymma is used to treat fever, skin ailments, and body, joint, and facial muscle pains, and it is also applied as cosmetic. Biological assays conducted with the A. axillarum crude leaf ethanol extract have indicated leishmanicidal activity and absence of cytotoxicity. This study aimed to analyze the A. axillarum leaf ethanol crude extract by high-performance liquid chromatography-high-resolution mass spectrometry- diode array detector (HPLC-HRMS-DAD) and to evaluate the leishmanicidal and cytotoxic activities of this crude extract, its fractions, and isolated compounds. HPLC-HRMS-DAD analysis of this extract revealed that it consisted mainly of flavonoids, with nine major compounds. Extract purification yielded 4-hydroxy-N-methylproline, 6-β-hydroxyipolamiide, quercetin-3-O-robinobioside, hyperin, isorhamnetin-3-O-robinobioside, and 3’-O-methylhyperin, which were identified by Nuclear Magnetic Resonance. The isolated compounds were inactive against Leishmania amazonensis promastigotes and human lung fibroblast cells.