Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/204952 |
Resumo: | Adenocalymma axillarum (K.Schum.) L.G. Lohmann is a liana belonging to the family Bignoniaceae. In traditional medicine, the genus Adenocalymma is used to treat fever, skin ailments, and body, joint, and facial muscle pains, and it is also applied as cosmetic. Biological assays conducted with the A. axillarum crude leaf ethanol extract have indicated leishmanicidal activity and absence of cytotoxicity. This study aimed to analyze the A. axillarum leaf ethanol crude extract by high-performance liquid chromatography-high-resolution mass spectrometry- diode array detector (HPLC-HRMS-DAD) and to evaluate the leishmanicidal and cytotoxic activities of this crude extract, its fractions, and isolated compounds. HPLC-HRMS-DAD analysis of this extract revealed that it consisted mainly of flavonoids, with nine major compounds. Extract purification yielded 4-hydroxy-N-methylproline, 6-β-hydroxyipolamiide, quercetin-3-O-robinobioside, hyperin, isorhamnetin-3-O-robinobioside, and 3’-O-methylhyperin, which were identified by Nuclear Magnetic Resonance. The isolated compounds were inactive against Leishmania amazonensis promastigotes and human lung fibroblast cells. |
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Brazilian Journal of Pharmaceutical Sciences |
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Chemical study of Adenocalymma axillarum crude leaf extract and isolated compoundsCytotoxicityFlavonolHPLC-HRMS-DAD IridoidLeishmanicidalAdenocalymma axillarum (K.Schum.) L.G. Lohmann is a liana belonging to the family Bignoniaceae. In traditional medicine, the genus Adenocalymma is used to treat fever, skin ailments, and body, joint, and facial muscle pains, and it is also applied as cosmetic. Biological assays conducted with the A. axillarum crude leaf ethanol extract have indicated leishmanicidal activity and absence of cytotoxicity. This study aimed to analyze the A. axillarum leaf ethanol crude extract by high-performance liquid chromatography-high-resolution mass spectrometry- diode array detector (HPLC-HRMS-DAD) and to evaluate the leishmanicidal and cytotoxic activities of this crude extract, its fractions, and isolated compounds. HPLC-HRMS-DAD analysis of this extract revealed that it consisted mainly of flavonoids, with nine major compounds. Extract purification yielded 4-hydroxy-N-methylproline, 6-β-hydroxyipolamiide, quercetin-3-O-robinobioside, hyperin, isorhamnetin-3-O-robinobioside, and 3’-O-methylhyperin, which were identified by Nuclear Magnetic Resonance. The isolated compounds were inactive against Leishmania amazonensis promastigotes and human lung fibroblast cells.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2022-11-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/20495210.1590/s2175-97902022e20278 Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022)Brazilian Journal of Pharmaceutical Sciences; v. 58 (2022)Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022)2175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/204952/194444Copyright (c) 2022 Brazilian Journal of Pharmaceutical Scienceshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCampos Andrade, João PauloAlvarenga Alves, Osvaine JuniorCosta, Marian CarolineMelleiro Gimenez, Valéria MariaSilva Squarisi, IaraDiniz Nicolella, HeloizaCintra Pagotti, MarianaCrispim Tavares, DeniseCunha, Wilson RobertoAndrade e Silva, Márcio LuísJanuario, Ana HelenaGuidi Magalhães, LizandraMendonça Pauletti, Patrícia2023-05-25T12:56:45Zoai:revistas.usp.br:article/204952Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2023-05-25T12:56:45Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds |
title |
Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds |
spellingShingle |
Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds Campos Andrade, João Paulo Cytotoxicity Flavonol HPLC-HRMS-DAD Iridoid Leishmanicidal |
title_short |
Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds |
title_full |
Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds |
title_fullStr |
Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds |
title_full_unstemmed |
Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds |
title_sort |
Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds |
author |
Campos Andrade, João Paulo |
author_facet |
Campos Andrade, João Paulo Alvarenga Alves, Osvaine Junior Costa, Marian Caroline Melleiro Gimenez, Valéria Maria Silva Squarisi, Iara Diniz Nicolella, Heloiza Cintra Pagotti, Mariana Crispim Tavares, Denise Cunha, Wilson Roberto Andrade e Silva, Márcio Luís Januario, Ana Helena Guidi Magalhães, Lizandra Mendonça Pauletti, Patrícia |
author_role |
author |
author2 |
Alvarenga Alves, Osvaine Junior Costa, Marian Caroline Melleiro Gimenez, Valéria Maria Silva Squarisi, Iara Diniz Nicolella, Heloiza Cintra Pagotti, Mariana Crispim Tavares, Denise Cunha, Wilson Roberto Andrade e Silva, Márcio Luís Januario, Ana Helena Guidi Magalhães, Lizandra Mendonça Pauletti, Patrícia |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Campos Andrade, João Paulo Alvarenga Alves, Osvaine Junior Costa, Marian Caroline Melleiro Gimenez, Valéria Maria Silva Squarisi, Iara Diniz Nicolella, Heloiza Cintra Pagotti, Mariana Crispim Tavares, Denise Cunha, Wilson Roberto Andrade e Silva, Márcio Luís Januario, Ana Helena Guidi Magalhães, Lizandra Mendonça Pauletti, Patrícia |
dc.subject.por.fl_str_mv |
Cytotoxicity Flavonol HPLC-HRMS-DAD Iridoid Leishmanicidal |
topic |
Cytotoxicity Flavonol HPLC-HRMS-DAD Iridoid Leishmanicidal |
description |
Adenocalymma axillarum (K.Schum.) L.G. Lohmann is a liana belonging to the family Bignoniaceae. In traditional medicine, the genus Adenocalymma is used to treat fever, skin ailments, and body, joint, and facial muscle pains, and it is also applied as cosmetic. Biological assays conducted with the A. axillarum crude leaf ethanol extract have indicated leishmanicidal activity and absence of cytotoxicity. This study aimed to analyze the A. axillarum leaf ethanol crude extract by high-performance liquid chromatography-high-resolution mass spectrometry- diode array detector (HPLC-HRMS-DAD) and to evaluate the leishmanicidal and cytotoxic activities of this crude extract, its fractions, and isolated compounds. HPLC-HRMS-DAD analysis of this extract revealed that it consisted mainly of flavonoids, with nine major compounds. Extract purification yielded 4-hydroxy-N-methylproline, 6-β-hydroxyipolamiide, quercetin-3-O-robinobioside, hyperin, isorhamnetin-3-O-robinobioside, and 3’-O-methylhyperin, which were identified by Nuclear Magnetic Resonance. The isolated compounds were inactive against Leishmania amazonensis promastigotes and human lung fibroblast cells. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-25 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/204952 10.1590/s2175-97902022e20278 |
url |
https://www.revistas.usp.br/bjps/article/view/204952 |
identifier_str_mv |
10.1590/s2175-97902022e20278 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/204952/194444 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022) Brazilian Journal of Pharmaceutical Sciences; v. 58 (2022) Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022) 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1800222916491608064 |