Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds

Detalhes bibliográficos
Autor(a) principal: Campos Andrade, João Paulo
Data de Publicação: 2022
Outros Autores: Alvarenga Alves, Osvaine Junior, Costa, Marian Caroline, Melleiro Gimenez, Valéria Maria, Silva Squarisi, Iara, Diniz Nicolella, Heloiza, Cintra Pagotti, Mariana, Crispim Tavares, Denise, Cunha, Wilson Roberto, Andrade e Silva, Márcio Luís, Januario, Ana Helena, Guidi Magalhães, Lizandra, Mendonça Pauletti, Patrícia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Pharmaceutical Sciences
Texto Completo: https://www.revistas.usp.br/bjps/article/view/204952
Resumo: Adenocalymma axillarum (K.Schum.) L.G. Lohmann is a liana belonging to the family Bignoniaceae. In traditional medicine, the genus Adenocalymma is used to treat fever, skin ailments, and body, joint, and facial muscle pains, and it is also applied as cosmetic. Biological assays conducted with the A. axillarum crude leaf ethanol extract have indicated leishmanicidal activity and absence of cytotoxicity. This study aimed to analyze the A. axillarum leaf ethanol crude extract by high-performance liquid chromatography-high-resolution mass spectrometry- diode array detector (HPLC-HRMS-DAD) and to evaluate the leishmanicidal and cytotoxic activities of this crude extract, its fractions, and isolated compounds. HPLC-HRMS-DAD analysis of this extract revealed that it consisted mainly of flavonoids, with nine major compounds. Extract purification yielded 4-hydroxy-N-methylproline, 6-β-hydroxyipolamiide, quercetin-3-O-robinobioside, hyperin, isorhamnetin-3-O-robinobioside, and 3’-O-methylhyperin, which were identified by Nuclear Magnetic Resonance. The isolated compounds were inactive against Leishmania amazonensis promastigotes and human lung fibroblast cells.
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spelling Chemical study of Adenocalymma axillarum crude leaf extract and isolated compoundsCytotoxicityFlavonolHPLC-HRMS-DAD IridoidLeishmanicidalAdenocalymma axillarum (K.Schum.) L.G. Lohmann is a liana belonging to the family Bignoniaceae. In traditional medicine, the genus Adenocalymma is used to treat fever, skin ailments, and body, joint, and facial muscle pains, and it is also applied as cosmetic. Biological assays conducted with the A. axillarum crude leaf ethanol extract have indicated leishmanicidal activity and absence of cytotoxicity. This study aimed to analyze the A. axillarum leaf ethanol crude extract by high-performance liquid chromatography-high-resolution mass spectrometry- diode array detector (HPLC-HRMS-DAD) and to evaluate the leishmanicidal and cytotoxic activities of this crude extract, its fractions, and isolated compounds. HPLC-HRMS-DAD analysis of this extract revealed that it consisted mainly of flavonoids, with nine major compounds. Extract purification yielded 4-hydroxy-N-methylproline, 6-β-hydroxyipolamiide, quercetin-3-O-robinobioside, hyperin, isorhamnetin-3-O-robinobioside, and 3’-O-methylhyperin, which were identified by Nuclear Magnetic Resonance. The isolated compounds were inactive against Leishmania amazonensis promastigotes and human lung fibroblast cells.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2022-11-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/20495210.1590/s2175-97902022e20278 Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022)Brazilian Journal of Pharmaceutical Sciences; v. 58 (2022)Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022)2175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/204952/194444Copyright (c) 2022 Brazilian Journal of Pharmaceutical Scienceshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCampos Andrade, João PauloAlvarenga Alves, Osvaine JuniorCosta, Marian CarolineMelleiro Gimenez, Valéria MariaSilva Squarisi, IaraDiniz Nicolella, HeloizaCintra Pagotti, MarianaCrispim Tavares, DeniseCunha, Wilson RobertoAndrade e Silva, Márcio LuísJanuario, Ana HelenaGuidi Magalhães, LizandraMendonça Pauletti, Patrícia2023-05-25T12:56:45Zoai:revistas.usp.br:article/204952Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2023-05-25T12:56:45Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds
title Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds
spellingShingle Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds
Campos Andrade, João Paulo
Cytotoxicity
Flavonol
HPLC-HRMS-DAD
Iridoid
Leishmanicidal
title_short Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds
title_full Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds
title_fullStr Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds
title_full_unstemmed Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds
title_sort Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds
author Campos Andrade, João Paulo
author_facet Campos Andrade, João Paulo
Alvarenga Alves, Osvaine Junior
Costa, Marian Caroline
Melleiro Gimenez, Valéria Maria
Silva Squarisi, Iara
Diniz Nicolella, Heloiza
Cintra Pagotti, Mariana
Crispim Tavares, Denise
Cunha, Wilson Roberto
Andrade e Silva, Márcio Luís
Januario, Ana Helena
Guidi Magalhães, Lizandra
Mendonça Pauletti, Patrícia
author_role author
author2 Alvarenga Alves, Osvaine Junior
Costa, Marian Caroline
Melleiro Gimenez, Valéria Maria
Silva Squarisi, Iara
Diniz Nicolella, Heloiza
Cintra Pagotti, Mariana
Crispim Tavares, Denise
Cunha, Wilson Roberto
Andrade e Silva, Márcio Luís
Januario, Ana Helena
Guidi Magalhães, Lizandra
Mendonça Pauletti, Patrícia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Campos Andrade, João Paulo
Alvarenga Alves, Osvaine Junior
Costa, Marian Caroline
Melleiro Gimenez, Valéria Maria
Silva Squarisi, Iara
Diniz Nicolella, Heloiza
Cintra Pagotti, Mariana
Crispim Tavares, Denise
Cunha, Wilson Roberto
Andrade e Silva, Márcio Luís
Januario, Ana Helena
Guidi Magalhães, Lizandra
Mendonça Pauletti, Patrícia
dc.subject.por.fl_str_mv Cytotoxicity
Flavonol
HPLC-HRMS-DAD
Iridoid
Leishmanicidal
topic Cytotoxicity
Flavonol
HPLC-HRMS-DAD
Iridoid
Leishmanicidal
description Adenocalymma axillarum (K.Schum.) L.G. Lohmann is a liana belonging to the family Bignoniaceae. In traditional medicine, the genus Adenocalymma is used to treat fever, skin ailments, and body, joint, and facial muscle pains, and it is also applied as cosmetic. Biological assays conducted with the A. axillarum crude leaf ethanol extract have indicated leishmanicidal activity and absence of cytotoxicity. This study aimed to analyze the A. axillarum leaf ethanol crude extract by high-performance liquid chromatography-high-resolution mass spectrometry- diode array detector (HPLC-HRMS-DAD) and to evaluate the leishmanicidal and cytotoxic activities of this crude extract, its fractions, and isolated compounds. HPLC-HRMS-DAD analysis of this extract revealed that it consisted mainly of flavonoids, with nine major compounds. Extract purification yielded 4-hydroxy-N-methylproline, 6-β-hydroxyipolamiide, quercetin-3-O-robinobioside, hyperin, isorhamnetin-3-O-robinobioside, and 3’-O-methylhyperin, which were identified by Nuclear Magnetic Resonance. The isolated compounds were inactive against Leishmania amazonensis promastigotes and human lung fibroblast cells.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-25
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjps/article/view/204952
10.1590/s2175-97902022e20278
url https://www.revistas.usp.br/bjps/article/view/204952
identifier_str_mv 10.1590/s2175-97902022e20278
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjps/article/view/204952/194444
dc.rights.driver.fl_str_mv Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
dc.source.none.fl_str_mv Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022)
Brazilian Journal of Pharmaceutical Sciences; v. 58 (2022)
Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022)
2175-9790
1984-8250
reponame:Brazilian Journal of Pharmaceutical Sciences
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Pharmaceutical Sciences
collection Brazilian Journal of Pharmaceutical Sciences
repository.name.fl_str_mv Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)
repository.mail.fl_str_mv bjps@usp.br||elizabeth.igne@gmail.com
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