Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/131418 |
Resumo: | In this study, conditions were optimized for development of a simple RP-HPLC method for simultaneous analysis of gatifloxacin and dexamethasone in different matrices like pharmaceuticals, human serum and urine. Good separation of gatifloxacin and dexamethasone from the induced degradation products was accomplished using C8 as stationary phase; 0.02 M phosphate buffer (pH 3.0) and methanol (42:58 v/v) as mobile phase. The concentration was measured with DAD at 270 nm. Linearity was observed in the range of 0.000040-0.000280 mol/L for gatifloxacin (r2≥0.999) and 0.000013-0.000091 mol/L for dexamethasone (r2≥0.999). Both the analyte peaks were completely separated from the peaks of induced degradation products as indicated by the peak purity index (≥0.9999 for both analytes). The optimized method is recommended to be used for concurrent analysis of gatifloxacin and dexamethasone in different matrices. |
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Brazilian Journal of Pharmaceutical Sciences |
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Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combinationGatifloxacinDexamethasoneHigh performance liquid chromatographyValidationPlasmaDegradation products.In this study, conditions were optimized for development of a simple RP-HPLC method for simultaneous analysis of gatifloxacin and dexamethasone in different matrices like pharmaceuticals, human serum and urine. Good separation of gatifloxacin and dexamethasone from the induced degradation products was accomplished using C8 as stationary phase; 0.02 M phosphate buffer (pH 3.0) and methanol (42:58 v/v) as mobile phase. The concentration was measured with DAD at 270 nm. Linearity was observed in the range of 0.000040-0.000280 mol/L for gatifloxacin (r2≥0.999) and 0.000013-0.000091 mol/L for dexamethasone (r2≥0.999). Both the analyte peaks were completely separated from the peaks of induced degradation products as indicated by the peak purity index (≥0.9999 for both analytes). The optimized method is recommended to be used for concurrent analysis of gatifloxacin and dexamethasone in different matrices.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/13141810.1590/s2175-97902017000115177Brazilian Journal of Pharmaceutical Sciences; Vol. 53 Núm. 1 (2017); e15177-Brazilian Journal of Pharmaceutical Sciences; v. 53 n. 1 (2017); e15177-Brazilian Journal of Pharmaceutical Sciences; Vol. 53 No. 1 (2017); e15177-2175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/131418/127798Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)info:eu-repo/semantics/openAccessRazzaq, Syed NaeemAshfaq, MuhammadKhan, Islam UllahMariam, IrfanaRazzaq, Syed SaleemMustafa, GhulamZubair, Muhammad2017-04-20T20:28:50Zoai:revistas.usp.br:article/131418Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2017-04-20T20:28:50Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination |
title |
Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination |
spellingShingle |
Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination Razzaq, Syed Naeem Gatifloxacin Dexamethasone High performance liquid chromatography Validation Plasma Degradation products. |
title_short |
Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination |
title_full |
Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination |
title_fullStr |
Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination |
title_full_unstemmed |
Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination |
title_sort |
Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination |
author |
Razzaq, Syed Naeem |
author_facet |
Razzaq, Syed Naeem Ashfaq, Muhammad Khan, Islam Ullah Mariam, Irfana Razzaq, Syed Saleem Mustafa, Ghulam Zubair, Muhammad |
author_role |
author |
author2 |
Ashfaq, Muhammad Khan, Islam Ullah Mariam, Irfana Razzaq, Syed Saleem Mustafa, Ghulam Zubair, Muhammad |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Razzaq, Syed Naeem Ashfaq, Muhammad Khan, Islam Ullah Mariam, Irfana Razzaq, Syed Saleem Mustafa, Ghulam Zubair, Muhammad |
dc.subject.por.fl_str_mv |
Gatifloxacin Dexamethasone High performance liquid chromatography Validation Plasma Degradation products. |
topic |
Gatifloxacin Dexamethasone High performance liquid chromatography Validation Plasma Degradation products. |
description |
In this study, conditions were optimized for development of a simple RP-HPLC method for simultaneous analysis of gatifloxacin and dexamethasone in different matrices like pharmaceuticals, human serum and urine. Good separation of gatifloxacin and dexamethasone from the induced degradation products was accomplished using C8 as stationary phase; 0.02 M phosphate buffer (pH 3.0) and methanol (42:58 v/v) as mobile phase. The concentration was measured with DAD at 270 nm. Linearity was observed in the range of 0.000040-0.000280 mol/L for gatifloxacin (r2≥0.999) and 0.000013-0.000091 mol/L for dexamethasone (r2≥0.999). Both the analyte peaks were completely separated from the peaks of induced degradation products as indicated by the peak purity index (≥0.9999 for both analytes). The optimized method is recommended to be used for concurrent analysis of gatifloxacin and dexamethasone in different matrices. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/131418 10.1590/s2175-97902017000115177 |
url |
https://www.revistas.usp.br/bjps/article/view/131418 |
identifier_str_mv |
10.1590/s2175-97902017000115177 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/131418/127798 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 53 Núm. 1 (2017); e15177- Brazilian Journal of Pharmaceutical Sciences; v. 53 n. 1 (2017); e15177- Brazilian Journal of Pharmaceutical Sciences; Vol. 53 No. 1 (2017); e15177- 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1800222912905478144 |