Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination

Detalhes bibliográficos
Autor(a) principal: Razzaq, Syed Naeem
Data de Publicação: 2017
Outros Autores: Ashfaq, Muhammad, Khan, Islam Ullah, Mariam, Irfana, Razzaq, Syed Saleem, Mustafa, Ghulam, Zubair, Muhammad
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Pharmaceutical Sciences
Texto Completo: https://www.revistas.usp.br/bjps/article/view/131418
Resumo: In this study, conditions were optimized for development of a simple RP-HPLC method for simultaneous analysis of gatifloxacin and dexamethasone in different matrices like pharmaceuticals, human serum and urine. Good separation of gatifloxacin and dexamethasone from the induced degradation products was accomplished using C8 as stationary phase; 0.02 M phosphate buffer (pH 3.0) and methanol (42:58 v/v) as mobile phase. The concentration was measured with DAD at 270 nm. Linearity was observed in the range of 0.000040-0.000280 mol/L for gatifloxacin (r2≥0.999) and 0.000013-0.000091 mol/L for dexamethasone (r2≥0.999). Both the analyte peaks were completely separated from the peaks of induced degradation products as indicated by the peak purity index (≥0.9999 for both analytes). The optimized method is recommended to be used for concurrent analysis of gatifloxacin and dexamethasone in different matrices.
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spelling Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combinationGatifloxacinDexamethasoneHigh performance liquid chromatographyValidationPlasmaDegradation products.In this study, conditions were optimized for development of a simple RP-HPLC method for simultaneous analysis of gatifloxacin and dexamethasone in different matrices like pharmaceuticals, human serum and urine. Good separation of gatifloxacin and dexamethasone from the induced degradation products was accomplished using C8 as stationary phase; 0.02 M phosphate buffer (pH 3.0) and methanol (42:58 v/v) as mobile phase. The concentration was measured with DAD at 270 nm. Linearity was observed in the range of 0.000040-0.000280 mol/L for gatifloxacin (r2≥0.999) and 0.000013-0.000091 mol/L for dexamethasone (r2≥0.999). Both the analyte peaks were completely separated from the peaks of induced degradation products as indicated by the peak purity index (≥0.9999 for both analytes). The optimized method is recommended to be used for concurrent analysis of gatifloxacin and dexamethasone in different matrices.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/13141810.1590/s2175-97902017000115177Brazilian Journal of Pharmaceutical Sciences; Vol. 53 Núm. 1 (2017); e15177-Brazilian Journal of Pharmaceutical Sciences; v. 53 n. 1 (2017); e15177-Brazilian Journal of Pharmaceutical Sciences; Vol. 53 No. 1 (2017); e15177-2175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/131418/127798Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)info:eu-repo/semantics/openAccessRazzaq, Syed NaeemAshfaq, MuhammadKhan, Islam UllahMariam, IrfanaRazzaq, Syed SaleemMustafa, GhulamZubair, Muhammad2017-04-20T20:28:50Zoai:revistas.usp.br:article/131418Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2017-04-20T20:28:50Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination
title Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination
spellingShingle Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination
Razzaq, Syed Naeem
Gatifloxacin
Dexamethasone
High performance liquid chromatography
Validation
Plasma
Degradation products.
title_short Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination
title_full Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination
title_fullStr Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination
title_full_unstemmed Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination
title_sort Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination
author Razzaq, Syed Naeem
author_facet Razzaq, Syed Naeem
Ashfaq, Muhammad
Khan, Islam Ullah
Mariam, Irfana
Razzaq, Syed Saleem
Mustafa, Ghulam
Zubair, Muhammad
author_role author
author2 Ashfaq, Muhammad
Khan, Islam Ullah
Mariam, Irfana
Razzaq, Syed Saleem
Mustafa, Ghulam
Zubair, Muhammad
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Razzaq, Syed Naeem
Ashfaq, Muhammad
Khan, Islam Ullah
Mariam, Irfana
Razzaq, Syed Saleem
Mustafa, Ghulam
Zubair, Muhammad
dc.subject.por.fl_str_mv Gatifloxacin
Dexamethasone
High performance liquid chromatography
Validation
Plasma
Degradation products.
topic Gatifloxacin
Dexamethasone
High performance liquid chromatography
Validation
Plasma
Degradation products.
description In this study, conditions were optimized for development of a simple RP-HPLC method for simultaneous analysis of gatifloxacin and dexamethasone in different matrices like pharmaceuticals, human serum and urine. Good separation of gatifloxacin and dexamethasone from the induced degradation products was accomplished using C8 as stationary phase; 0.02 M phosphate buffer (pH 3.0) and methanol (42:58 v/v) as mobile phase. The concentration was measured with DAD at 270 nm. Linearity was observed in the range of 0.000040-0.000280 mol/L for gatifloxacin (r2≥0.999) and 0.000013-0.000091 mol/L for dexamethasone (r2≥0.999). Both the analyte peaks were completely separated from the peaks of induced degradation products as indicated by the peak purity index (≥0.9999 for both analytes). The optimized method is recommended to be used for concurrent analysis of gatifloxacin and dexamethasone in different matrices.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjps/article/view/131418
10.1590/s2175-97902017000115177
url https://www.revistas.usp.br/bjps/article/view/131418
identifier_str_mv 10.1590/s2175-97902017000115177
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjps/article/view/131418/127798
dc.rights.driver.fl_str_mv Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
dc.source.none.fl_str_mv Brazilian Journal of Pharmaceutical Sciences; Vol. 53 Núm. 1 (2017); e15177-
Brazilian Journal of Pharmaceutical Sciences; v. 53 n. 1 (2017); e15177-
Brazilian Journal of Pharmaceutical Sciences; Vol. 53 No. 1 (2017); e15177-
2175-9790
1984-8250
reponame:Brazilian Journal of Pharmaceutical Sciences
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Pharmaceutical Sciences
collection Brazilian Journal of Pharmaceutical Sciences
repository.name.fl_str_mv Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)
repository.mail.fl_str_mv bjps@usp.br||elizabeth.igne@gmail.com
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