Evaluation of the impact of orally administered carbohydrates on postprandial blood glucose levels in different pre-clinical models

• Autor(a) principal: Marques, Any de Castro Ruiz
• Data de Publicação: 2016
• Outros Autores: Schiavon, Fabiana Percinoto Monteiro, Travassos, Patricia Batista, Eik, Vanessa Fontana, Godoy, Guilherme, Schamber, Christiano Rodrigues, Bazotte, Roberto Barbosa
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Pharmaceutical Sciences
• DOI: 10.1590/s1984-82502016000400021
• Texto Completo: https://www.revistas.usp.br/bjps/article/view/128430

We developed a pre-clinical model in which to evaluate the impact of orally administered carbohydrates on postprandial blood glucose levels. For this purpose, we compared the effects of different carbohydrates with well-established glycemic indexes. We orally administered (gavage) increasing amounts (0.2, 0.4, 0.6, 0.8, and 1.0 g/kg) of sucrose and lactose to rats which had been fasted for 6 h or 15 h, respectively. In part of the experiments we administered frutose (gavagem). Three different models were compared for measuring postprandial blood glucose levels: a) evaluation of interstitial glucose concentrations by using a real time continuous glucose monitoring system; b) evaluation of glucose levels in blood obtained from the rat tail; c) evaluation of serum glucose levels in blood collected after decapitation. Our results showed that blood obtained from the tails of 15-h fasted rats was the best model in which to evaluate the effect of carbohydrates on postprandial blood glucose levels.