Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/131414 |
Resumo: | In the present study, a reversed-phase high-performance liquid chromatographic method has been used for the quantitative determination of lycorine in the aerial parts and bulbs of G. elwesii Hook. A simple method for the extraction of lycorine in low mass plant samples was employed utilizing pre-packed columns with diatomaceous earth (Extrelut(r)). The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:92.5:7.5, v/v/v) applied at a flow rate 1 mL min-1 using diode array detector. The content of lycorine in the bulbs and aerial parts of G. elwesii collected from Demirci (Manisa) was found as 0.130 and 0.162 %, respectively. Additionally, in the bulbs of the specimens collected from Sogucak (Balikesir), lycorine was quantified as 0.055 %, whereas in the aerial parts, it was determined as 0.006 %. The method was validated partially with respect to system specificity, linearity, accuracy, precision, limits of detection (LOD) and quantitation (LOQ). Validation procedures displayed that the method was specific, accurate and precise. |
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Brazilian Journal of Pharmaceutical Sciences |
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Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii HookAlkaloidsAmaryllidaceaePlant extractsHigh Performance Liquid Chromatography/method validationHigh Perfomance Liquid Chromatography/quantitative analysis.In the present study, a reversed-phase high-performance liquid chromatographic method has been used for the quantitative determination of lycorine in the aerial parts and bulbs of G. elwesii Hook. A simple method for the extraction of lycorine in low mass plant samples was employed utilizing pre-packed columns with diatomaceous earth (Extrelut(r)). The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:92.5:7.5, v/v/v) applied at a flow rate 1 mL min-1 using diode array detector. The content of lycorine in the bulbs and aerial parts of G. elwesii collected from Demirci (Manisa) was found as 0.130 and 0.162 %, respectively. Additionally, in the bulbs of the specimens collected from Sogucak (Balikesir), lycorine was quantified as 0.055 %, whereas in the aerial parts, it was determined as 0.006 %. The method was validated partially with respect to system specificity, linearity, accuracy, precision, limits of detection (LOD) and quantitation (LOQ). Validation procedures displayed that the method was specific, accurate and precise.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/13141410.1590/s2175-97902017000115063Brazilian Journal of Pharmaceutical Sciences; Vol. 53 Núm. 1 (2017); e15063-Brazilian Journal of Pharmaceutical Sciences; v. 53 n. 1 (2017); e15063-Brazilian Journal of Pharmaceutical Sciences; Vol. 53 No. 1 (2017); e15063-2175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/131414/127794Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)info:eu-repo/semantics/openAccessEmir, AhmetEmir, CerenBozkurt, BuketOnur, Mustafa AliSomer, Nehir UnverKaya, Gulen Irem2017-04-20T20:28:50Zoai:revistas.usp.br:article/131414Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2017-04-20T20:28:50Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook |
title |
Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook |
spellingShingle |
Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook Emir, Ahmet Alkaloids Amaryllidaceae Plant extracts High Performance Liquid Chromatography/method validation High Perfomance Liquid Chromatography/quantitative analysis. |
title_short |
Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook |
title_full |
Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook |
title_fullStr |
Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook |
title_full_unstemmed |
Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook |
title_sort |
Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook |
author |
Emir, Ahmet |
author_facet |
Emir, Ahmet Emir, Ceren Bozkurt, Buket Onur, Mustafa Ali Somer, Nehir Unver Kaya, Gulen Irem |
author_role |
author |
author2 |
Emir, Ceren Bozkurt, Buket Onur, Mustafa Ali Somer, Nehir Unver Kaya, Gulen Irem |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Emir, Ahmet Emir, Ceren Bozkurt, Buket Onur, Mustafa Ali Somer, Nehir Unver Kaya, Gulen Irem |
dc.subject.por.fl_str_mv |
Alkaloids Amaryllidaceae Plant extracts High Performance Liquid Chromatography/method validation High Perfomance Liquid Chromatography/quantitative analysis. |
topic |
Alkaloids Amaryllidaceae Plant extracts High Performance Liquid Chromatography/method validation High Perfomance Liquid Chromatography/quantitative analysis. |
description |
In the present study, a reversed-phase high-performance liquid chromatographic method has been used for the quantitative determination of lycorine in the aerial parts and bulbs of G. elwesii Hook. A simple method for the extraction of lycorine in low mass plant samples was employed utilizing pre-packed columns with diatomaceous earth (Extrelut(r)). The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:92.5:7.5, v/v/v) applied at a flow rate 1 mL min-1 using diode array detector. The content of lycorine in the bulbs and aerial parts of G. elwesii collected from Demirci (Manisa) was found as 0.130 and 0.162 %, respectively. Additionally, in the bulbs of the specimens collected from Sogucak (Balikesir), lycorine was quantified as 0.055 %, whereas in the aerial parts, it was determined as 0.006 %. The method was validated partially with respect to system specificity, linearity, accuracy, precision, limits of detection (LOD) and quantitation (LOQ). Validation procedures displayed that the method was specific, accurate and precise. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/131414 10.1590/s2175-97902017000115063 |
url |
https://www.revistas.usp.br/bjps/article/view/131414 |
identifier_str_mv |
10.1590/s2175-97902017000115063 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/131414/127794 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 53 Núm. 1 (2017); e15063- Brazilian Journal of Pharmaceutical Sciences; v. 53 n. 1 (2017); e15063- Brazilian Journal of Pharmaceutical Sciences; Vol. 53 No. 1 (2017); e15063- 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1800222912898138112 |