Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis

Detalhes bibliográficos
Autor(a) principal: Botelho, D.M.S.
Data de Publicação: 2021
Outros Autores: Resende, M.L.V., Teixeira, A.R., Santiago, W.D., Pozza, E.A., Moreira, S.I., Ramos, J.B., Aquino, S.O., Ferreira, A.G., Reichel, T., Guerra-Guimarães, Leonor
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/23296
Resumo: Cercosporin has excellent properties of photosensitization that have been widely used in organophotocatalyst and photodynamic therapy as well as an antimicrobial agent. Therefore, there is a need to quantify it accurately with accessible methods. A comparative analysis of cercosporin quantification obtained by spectrophotometry (SPEC) and high-performance liquid chromatography (HPLC) was performed for nineteen Cercospora coffeicola isolates from different coffee-producing municipalities in Brazil. Image analysis of cercosporin crystals was performed in isolates with either high or low production of the toxin. Our results show that SPEC and HPLC are equally valid for the cercosporin evaluation of C. coffeicola cultures grown in vitro. The isolates with high cercosporin production had a higher crystal number and size when compared to the one with low cercosporin production
id RCAP_741e1bac14d47d4a86ba31cd30b63515
oai_identifier_str oai:www.repository.utl.pt:10400.5/23296
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysismicroscopytoxinhigh-performance liquid chromatographymethodsCercosporin has excellent properties of photosensitization that have been widely used in organophotocatalyst and photodynamic therapy as well as an antimicrobial agent. Therefore, there is a need to quantify it accurately with accessible methods. A comparative analysis of cercosporin quantification obtained by spectrophotometry (SPEC) and high-performance liquid chromatography (HPLC) was performed for nineteen Cercospora coffeicola isolates from different coffee-producing municipalities in Brazil. Image analysis of cercosporin crystals was performed in isolates with either high or low production of the toxin. Our results show that SPEC and HPLC are equally valid for the cercosporin evaluation of C. coffeicola cultures grown in vitro. The isolates with high cercosporin production had a higher crystal number and size when compared to the one with low cercosporin productionInstitute of Chemistry, Slovak Academy of SciencesRepositório da Universidade de LisboaBotelho, D.M.S.Resende, M.L.V.Teixeira, A.R.Santiago, W.D.Pozza, E.A.Moreira, S.I.Ramos, J.B.Aquino, S.O.Ferreira, A.G.Reichel, T.Guerra-Guimarães, Leonor2022-01-28T11:29:44Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/23296engBotelho, D.M.S.; de Resende, M.L.V.; Teixeira, A.R; Santiago, Wilder Douglas; Pozza, Edson Ampélio; Moreira, Silvino Intra; Ramos, Juliana Barros; de Aquino, Sinara Oliveira; Ferreira, Antônio Gilberto; Reichel, Tharyn; Guerra-Guimarães, Leonor (2021). Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis. Chemical Papershttps://doi.org/10.1007/s11696-021-01965-5info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-29T01:30:41Zoai:www.repository.utl.pt:10400.5/23296Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:07:33.638916Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis
title Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis
spellingShingle Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis
Botelho, D.M.S.
microscopy
toxin
high-performance liquid chromatography
methods
title_short Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis
title_full Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis
title_fullStr Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis
title_full_unstemmed Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis
title_sort Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis
author Botelho, D.M.S.
author_facet Botelho, D.M.S.
Resende, M.L.V.
Teixeira, A.R.
Santiago, W.D.
Pozza, E.A.
Moreira, S.I.
Ramos, J.B.
Aquino, S.O.
Ferreira, A.G.
Reichel, T.
Guerra-Guimarães, Leonor
author_role author
author2 Resende, M.L.V.
Teixeira, A.R.
Santiago, W.D.
Pozza, E.A.
Moreira, S.I.
Ramos, J.B.
Aquino, S.O.
Ferreira, A.G.
Reichel, T.
Guerra-Guimarães, Leonor
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Botelho, D.M.S.
Resende, M.L.V.
Teixeira, A.R.
Santiago, W.D.
Pozza, E.A.
Moreira, S.I.
Ramos, J.B.
Aquino, S.O.
Ferreira, A.G.
Reichel, T.
Guerra-Guimarães, Leonor
dc.subject.por.fl_str_mv microscopy
toxin
high-performance liquid chromatography
methods
topic microscopy
toxin
high-performance liquid chromatography
methods
description Cercosporin has excellent properties of photosensitization that have been widely used in organophotocatalyst and photodynamic therapy as well as an antimicrobial agent. Therefore, there is a need to quantify it accurately with accessible methods. A comparative analysis of cercosporin quantification obtained by spectrophotometry (SPEC) and high-performance liquid chromatography (HPLC) was performed for nineteen Cercospora coffeicola isolates from different coffee-producing municipalities in Brazil. Image analysis of cercosporin crystals was performed in isolates with either high or low production of the toxin. Our results show that SPEC and HPLC are equally valid for the cercosporin evaluation of C. coffeicola cultures grown in vitro. The isolates with high cercosporin production had a higher crystal number and size when compared to the one with low cercosporin production
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01T00:00:00Z
2022-01-28T11:29:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/23296
url http://hdl.handle.net/10400.5/23296
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Botelho, D.M.S.; de Resende, M.L.V.; Teixeira, A.R; Santiago, Wilder Douglas; Pozza, Edson Ampélio; Moreira, Silvino Intra; Ramos, Juliana Barros; de Aquino, Sinara Oliveira; Ferreira, Antônio Gilberto; Reichel, Tharyn; Guerra-Guimarães, Leonor (2021). Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis. Chemical Papers
https://doi.org/10.1007/s11696-021-01965-5
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Institute of Chemistry, Slovak Academy of Sciences
publisher.none.fl_str_mv Institute of Chemistry, Slovak Academy of Sciences
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799131169913044992

Registros relacionados