Action of mouthwashes on Staphylococcus spp: isolated in the saliva of community and hospitalized individuals
Main Author: | |
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Publication Date: | 2009 |
Other Authors: | , , , , |
Format: | Article |
Language: | eng |
Source: | Brazilian Journal of Pharmaceutical Sciences |
Download full: | https://www.revistas.usp.br/bjps/article/view/10712 |
Summary: | The use of mouthwashes in critical patients has been a source of concern for health professionals due to the diverse range of products, causing uncertainty about which is the most indicated. This study aimed to assess the susceptibility of Staphylococcus spp. isolated in the saliva of individuals from the community and patients in Intensive Care Units (ICU) as to antiseptic mouthwashes. The following oral antiseptics were assessed: cetylpyridinium chloride solution, Listerine® and Neen®. Calcium alginate swab was used for saliva collection to isolate Staphylococcus spp. Microbiological processing involved growth, isolation, strain identification and determination of MID (maximum inhibitory dilution). MID was considered the greatest dilution that completely inhibited the strains. The products efficacy was analyzed by a two-factor ANOVA repeated measures and by Bonferroni adjustments in multiple comparisons, considering a significance level of α=0.05. In total, 80 strains of Staphylococcus spp. were isolated, 40 from ICU patients and 40 from community individuals. MID results revealed that cetylpyridinium chloride solution presented better results in comparison to other products, that is, 39 (97.5%) strains from hospital patients with MID 1:128, and 37 (92.5%) of individuals from the community had MID 1:64. Neen® inhibited all strains in both groups at a dilution from 1:2 to 1:4. Listerine® presented the worst MID results, 65% of the strains from individuals from the community and 10% of hospital strains were not inhibited at a dilution of 1:2. |
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Action of mouthwashes on Staphylococcus spp: isolated in the saliva of community and hospitalized individuals Saúde bucalStaphylococcus sppAntissépticos bucaisHigiene bucalOral healthStaphylococcus sppMouthwashesOral hygiene The use of mouthwashes in critical patients has been a source of concern for health professionals due to the diverse range of products, causing uncertainty about which is the most indicated. This study aimed to assess the susceptibility of Staphylococcus spp. isolated in the saliva of individuals from the community and patients in Intensive Care Units (ICU) as to antiseptic mouthwashes. The following oral antiseptics were assessed: cetylpyridinium chloride solution, Listerine® and Neen®. Calcium alginate swab was used for saliva collection to isolate Staphylococcus spp. Microbiological processing involved growth, isolation, strain identification and determination of MID (maximum inhibitory dilution). MID was considered the greatest dilution that completely inhibited the strains. The products efficacy was analyzed by a two-factor ANOVA repeated measures and by Bonferroni adjustments in multiple comparisons, considering a significance level of α=0.05. In total, 80 strains of Staphylococcus spp. were isolated, 40 from ICU patients and 40 from community individuals. MID results revealed that cetylpyridinium chloride solution presented better results in comparison to other products, that is, 39 (97.5%) strains from hospital patients with MID 1:128, and 37 (92.5%) of individuals from the community had MID 1:64. Neen® inhibited all strains in both groups at a dilution from 1:2 to 1:4. Listerine® presented the worst MID results, 65% of the strains from individuals from the community and 10% of hospital strains were not inhibited at a dilution of 1:2. O uso de antissépticos bucais tem sido uma das preocupações dos profissionais de saúde considerando a diversidade de produtos, o que traz a insegurança sobre qual é o mais adequado. Objetivou-se avaliar a suscetibilidade de Staphylococcus spp. isolado da saliva de indivíduos adultos da comunidade e do hospital frente a antissépticos bucais. Os antissépticos avaliados foram: solução de Cloreto de Cetilpiridínio, Listerine® e Neen®. Na coleta de saliva para o isolamento do Staphylococcus spp. utilizou-se o swab de alginato de cálcio. O processamento microbiológico envolveu o crescimento, isolamento, identificação das cepas, determinação da DIM. Foi considerada DIM a maior diluição que inibiu completamente as cepas. A eficácia dos produtos foi avaliada por meio da análise de variância (ANOVA) bilateral com medidas repetidas, e pelo método de ajuste de Bonferroni nas comparações múltiplas, com um nível de significância α=0,05. Totalizou-se 80 cepas de Staphylococcus spp. sendo 40 isoladas de pacientes da UTI e 40 de indivíduos da comunidade. Comparando os resultados é possível verificar que a solução de cloreto de cetilpiridínio apresentou melhores resultados em relação aos demais produtos, ou seja, 39 (97,5%) das cepas hospitalares DIM 1:128 e da comunidade 37 (92,5%) DIM 1:64. Para Neen® nos dois grupos a DIM foi de 1:2 e 1:4. Listerine® apresentou o pior resultado em termos da DIM, sendo que 65% das cepas da comunidade e 10% hospitalar não foram inibidas na diluição 1:2. Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2009-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/1071210.1590/S1984-82502009000300021Brazilian Journal of Pharmaceutical Sciences; Vol. 45 No. 3 (2009); 551-557 Brazilian Journal of Pharmaceutical Sciences; v. 45 n. 3 (2009); 551-557 Brazilian Journal of Pharmaceutical Sciences; Vol. 45 Núm. 3 (2009); 551-557 2175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/10712/12480Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)info:eu-repo/semantics/openAccessAndrade, Denise deSouza, Paula Regina deBeraldo, Carolina ContadorWatanabe, EvandroLima, Mery ElenHaas, Vanderlei José2012-05-12T16:02:09Zoai:revistas.usp.br:article/10712Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2012-05-12T16:02:09Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Action of mouthwashes on Staphylococcus spp: isolated in the saliva of community and hospitalized individuals |
title |
Action of mouthwashes on Staphylococcus spp: isolated in the saliva of community and hospitalized individuals |
spellingShingle |
Action of mouthwashes on Staphylococcus spp: isolated in the saliva of community and hospitalized individuals Andrade, Denise de Saúde bucal Staphylococcus spp Antissépticos bucais Higiene bucal Oral health Staphylococcus spp Mouthwashes Oral hygiene |
title_short |
Action of mouthwashes on Staphylococcus spp: isolated in the saliva of community and hospitalized individuals |
title_full |
Action of mouthwashes on Staphylococcus spp: isolated in the saliva of community and hospitalized individuals |
title_fullStr |
Action of mouthwashes on Staphylococcus spp: isolated in the saliva of community and hospitalized individuals |
title_full_unstemmed |
Action of mouthwashes on Staphylococcus spp: isolated in the saliva of community and hospitalized individuals |
title_sort |
Action of mouthwashes on Staphylococcus spp: isolated in the saliva of community and hospitalized individuals |
author |
Andrade, Denise de |
author_facet |
Andrade, Denise de Souza, Paula Regina de Beraldo, Carolina Contador Watanabe, Evandro Lima, Mery Elen Haas, Vanderlei José |
author_role |
author |
author2 |
Souza, Paula Regina de Beraldo, Carolina Contador Watanabe, Evandro Lima, Mery Elen Haas, Vanderlei José |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Andrade, Denise de Souza, Paula Regina de Beraldo, Carolina Contador Watanabe, Evandro Lima, Mery Elen Haas, Vanderlei José |
dc.subject.por.fl_str_mv |
Saúde bucal Staphylococcus spp Antissépticos bucais Higiene bucal Oral health Staphylococcus spp Mouthwashes Oral hygiene |
topic |
Saúde bucal Staphylococcus spp Antissépticos bucais Higiene bucal Oral health Staphylococcus spp Mouthwashes Oral hygiene |
description |
The use of mouthwashes in critical patients has been a source of concern for health professionals due to the diverse range of products, causing uncertainty about which is the most indicated. This study aimed to assess the susceptibility of Staphylococcus spp. isolated in the saliva of individuals from the community and patients in Intensive Care Units (ICU) as to antiseptic mouthwashes. The following oral antiseptics were assessed: cetylpyridinium chloride solution, Listerine® and Neen®. Calcium alginate swab was used for saliva collection to isolate Staphylococcus spp. Microbiological processing involved growth, isolation, strain identification and determination of MID (maximum inhibitory dilution). MID was considered the greatest dilution that completely inhibited the strains. The products efficacy was analyzed by a two-factor ANOVA repeated measures and by Bonferroni adjustments in multiple comparisons, considering a significance level of α=0.05. In total, 80 strains of Staphylococcus spp. were isolated, 40 from ICU patients and 40 from community individuals. MID results revealed that cetylpyridinium chloride solution presented better results in comparison to other products, that is, 39 (97.5%) strains from hospital patients with MID 1:128, and 37 (92.5%) of individuals from the community had MID 1:64. Neen® inhibited all strains in both groups at a dilution from 1:2 to 1:4. Listerine® presented the worst MID results, 65% of the strains from individuals from the community and 10% of hospital strains were not inhibited at a dilution of 1:2. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/10712 10.1590/S1984-82502009000300021 |
url |
https://www.revistas.usp.br/bjps/article/view/10712 |
identifier_str_mv |
10.1590/S1984-82502009000300021 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/10712/12480 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 45 No. 3 (2009); 551-557 Brazilian Journal of Pharmaceutical Sciences; v. 45 n. 3 (2009); 551-557 Brazilian Journal of Pharmaceutical Sciences; Vol. 45 Núm. 3 (2009); 551-557 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1787713366408560640 |