Electrochemical immunosensing of low-density lipoprotein based on sol-gel encapsulation
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/212204 |
Resumo: | Lipoprotein monitoring is desirable in the management of medical conditions such as atherosclerotic cardiovascular disease and coronary artery disease, in which controlling the concentration of these chylomicrons is crucial. Current clinical methods are complex and present poor reproducibility between laboratories. For these reasons, recent guidelines discard the assessment of low-density lipoprotein cholesterol (LDL-C) as a routine analysis during lipid-lowering therapies. Concerning the importance of monitoring this parameter, the authors present an electrochemical immunosensor constructed from a simple and easy-to-reproduce platform that allows detecting and quantifying LDL nanoparticles directly from human serum samples. The performance of the biosensor was studied by scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The biosensing platform displays good stability and linearity between 30 mg dL-1 and 135 mg dL-1 with a detection limit of 20 mg dL-1. The proposed biosensor can be easily employed for monitoring LDL concentration in clinical treatments. |
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Brazilian Journal of Pharmaceutical Sciences |
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Electrochemical immunosensing of low-density lipoprotein based on sol-gel encapsulationApolipoproteinBiosensor; ElectrochemistryHypercholesterolemiaSelf-assembled monolayerSol-gelLipoprotein monitoring is desirable in the management of medical conditions such as atherosclerotic cardiovascular disease and coronary artery disease, in which controlling the concentration of these chylomicrons is crucial. Current clinical methods are complex and present poor reproducibility between laboratories. For these reasons, recent guidelines discard the assessment of low-density lipoprotein cholesterol (LDL-C) as a routine analysis during lipid-lowering therapies. Concerning the importance of monitoring this parameter, the authors present an electrochemical immunosensor constructed from a simple and easy-to-reproduce platform that allows detecting and quantifying LDL nanoparticles directly from human serum samples. The performance of the biosensor was studied by scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The biosensing platform displays good stability and linearity between 30 mg dL-1 and 135 mg dL-1 with a detection limit of 20 mg dL-1. The proposed biosensor can be easily employed for monitoring LDL concentration in clinical treatments.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2023-05-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/21220410.1590/s2175-97902023e22430Brazilian Journal of Pharmaceutical Sciences; Vol. 59 (2023)Brazilian Journal of Pharmaceutical Sciences; v. 59 (2023)Brazilian Journal of Pharmaceutical Sciences; Vol. 59 (2023)2175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/212204/194318Copyright (c) 2023 Brazilian Journal of Pharmaceutical Scienceshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessNascimento do Egito, Elton MaxMorales Frias, Isaac Aaron Oliveira, Maria D.L.Andrade, Cesar2023-05-24T17:42:03Zoai:revistas.usp.br:article/212204Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2023-05-24T17:42:03Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Electrochemical immunosensing of low-density lipoprotein based on sol-gel encapsulation |
title |
Electrochemical immunosensing of low-density lipoprotein based on sol-gel encapsulation |
spellingShingle |
Electrochemical immunosensing of low-density lipoprotein based on sol-gel encapsulation Nascimento do Egito, Elton Max Apolipoprotein Biosensor; Electrochemistry Hypercholesterolemia Self-assembled monolayer Sol-gel |
title_short |
Electrochemical immunosensing of low-density lipoprotein based on sol-gel encapsulation |
title_full |
Electrochemical immunosensing of low-density lipoprotein based on sol-gel encapsulation |
title_fullStr |
Electrochemical immunosensing of low-density lipoprotein based on sol-gel encapsulation |
title_full_unstemmed |
Electrochemical immunosensing of low-density lipoprotein based on sol-gel encapsulation |
title_sort |
Electrochemical immunosensing of low-density lipoprotein based on sol-gel encapsulation |
author |
Nascimento do Egito, Elton Max |
author_facet |
Nascimento do Egito, Elton Max Morales Frias, Isaac Aaron Oliveira, Maria D.L. Andrade, Cesar |
author_role |
author |
author2 |
Morales Frias, Isaac Aaron Oliveira, Maria D.L. Andrade, Cesar |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Nascimento do Egito, Elton Max Morales Frias, Isaac Aaron Oliveira, Maria D.L. Andrade, Cesar |
dc.subject.por.fl_str_mv |
Apolipoprotein Biosensor; Electrochemistry Hypercholesterolemia Self-assembled monolayer Sol-gel |
topic |
Apolipoprotein Biosensor; Electrochemistry Hypercholesterolemia Self-assembled monolayer Sol-gel |
description |
Lipoprotein monitoring is desirable in the management of medical conditions such as atherosclerotic cardiovascular disease and coronary artery disease, in which controlling the concentration of these chylomicrons is crucial. Current clinical methods are complex and present poor reproducibility between laboratories. For these reasons, recent guidelines discard the assessment of low-density lipoprotein cholesterol (LDL-C) as a routine analysis during lipid-lowering therapies. Concerning the importance of monitoring this parameter, the authors present an electrochemical immunosensor constructed from a simple and easy-to-reproduce platform that allows detecting and quantifying LDL nanoparticles directly from human serum samples. The performance of the biosensor was studied by scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The biosensing platform displays good stability and linearity between 30 mg dL-1 and 135 mg dL-1 with a detection limit of 20 mg dL-1. The proposed biosensor can be easily employed for monitoring LDL concentration in clinical treatments. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-05-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/212204 10.1590/s2175-97902023e22430 |
url |
https://www.revistas.usp.br/bjps/article/view/212204 |
identifier_str_mv |
10.1590/s2175-97902023e22430 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/212204/194318 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Brazilian Journal of Pharmaceutical Sciences https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Brazilian Journal of Pharmaceutical Sciences https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 59 (2023) Brazilian Journal of Pharmaceutical Sciences; v. 59 (2023) Brazilian Journal of Pharmaceutical Sciences; Vol. 59 (2023) 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1800222918107463680 |