A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum

Detalhes bibliográficos
Autor(a) principal: Jesus, Joilson Ramos
Data de Publicação: 2011
Outros Autores: Carvalho, Kellyanne dos Anjos, Fonseca, Rosana Anita da Silva, Oliveira, Geraldo Gileno de Sá, Melo, Stella Maria Barrouin, Neves, Neuza Maria Alcântara, Dutra, Rosa Amália Fireman
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/5366
Resumo: texto completo: restrito. p. 917–925
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spelling Jesus, Joilson RamosCarvalho, Kellyanne dos AnjosFonseca, Rosana Anita da SilvaOliveira, Geraldo Gileno de SáMelo, Stella Maria BarrouinNeves, Neuza Maria AlcântaraDutra, Rosa Amália FiremanJesus, Joilson RamosCarvalho, Kellyanne dos AnjosFonseca, Rosana Anita da SilvaOliveira, Geraldo Gileno de SáMelo, Stella Maria BarrouinNeves, Neuza Maria AlcântaraDutra, Rosa Amália Fireman2012-02-09T16:41:11Z20111618-2650http://www.repositorio.ufba.br/ri/handle/ri/5366v. 400.texto completo: restrito. p. 917–925The American visceral leishmaniasis is an important cause of morbidity and mortality in Brazil for both humans and dogs. Attempts to make a diagnosis of this disease need to be improved, especially in endemic areas, and in the tracking and screening of asymptomatic dogs, which are their main host in urban areas. A quartz crystal microbalance immunosensor for the diagnosis of the canine visceral leishmaniasis using a recombinant antigen of Leishmania chagasi (rLci2B-NH6) was devel- oped. The rLci2B-NH6 was tightly immobilized on a quartz crystal gold electrode by self-assembled monolayer based on short-chain length thiol. The strategy was the use of the antigen-histidine tail covalently linked to glutaral-dehyde performing a Schift base which permits a major exposure of epitopes and a reduced steric hindrance. The immunosensor showed good results regarding sensitivity and reproducibility, being able to distinguish positive and negative canine serum for L. chagasi. Furthermore, the immunosensor can be reused through exposure to sodium dodecyl sulfate solution, which promotes the dissociation of antigen–antibody binding, restoring the sensor surface with immobilized biologically active antigens for further analysis.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2012-02-09T16:41:11Z No. of bitstreams: 1 8951fulltext.pdf: 241170 bytes, checksum: 59707dfe33fe7c188ddf0ee00eb160e0 (MD5)Made available in DSpace on 2012-02-09T16:41:11Z (GMT). No. of bitstreams: 1 8951fulltext.pdf: 241170 bytes, checksum: 59707dfe33fe7c188ddf0ee00eb160e0 (MD5) Previous issue date: 2011DOI 10.1007/s00216-011-5136-7reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBALeishmania chagasiImmunosensorSelf-assembled monolayerPiezoelectricA piezoelectric immunosensor for Leishmania chagasi antibodies in canine serumAnalytical and Bioanalytical Chemistryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL8951fulltext.pdf8951fulltext.pdfapplication/pdf241170https://repositorio.ufba.br/bitstream/ri/5366/1/8951fulltext.pdf59707dfe33fe7c188ddf0ee00eb160e0MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/5366/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT8951fulltext.pdf.txt8951fulltext.pdf.txtExtracted texttext/plain37815https://repositorio.ufba.br/bitstream/ri/5366/3/8951fulltext.pdf.txtec9245cee839b5f3f2d42a622563c617MD53ri/53662022-07-05 14:04:02.447oai:repositorio.ufba.br:ri/5366VGVybW8gZGUgTGljZW7vv71hLCBu77+9byBleGNsdXNpdm8sIHBhcmEgbyBkZXDvv71zaXRvIG5vIHJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRkJBCgogICAgUGVsbyBwcm9jZXNzbyBkZSBzdWJtaXNz77+9byBkZSBkb2N1bWVudG9zLCBvIGF1dG9yIG91IHNldQpyZXByZXNlbnRhbnRlIGxlZ2FsLCBhbyBhY2VpdGFyIGVzc2UgdGVybW8gZGUgbGljZW7vv71hLCBjb25jZWRlIGFvClJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkYSBCYWhpYSBvIGRpcmVpdG8KZGUgbWFudGVyIHVtYSBj77+9cGlhIGVtIHNldSByZXBvc2l077+9cmlvIGNvbSBhIGZpbmFsaWRhZGUsIHByaW1laXJhLCAKZGUgcHJlc2VydmHvv73vv71vLiBFc3NlcyB0ZXJtb3MsIG7vv71vIGV4Y2x1c2l2b3MsIG1hbnTvv71tIG9zIGRpcmVpdG9zIGRlIAphdXRvci9jb3B5cmlnaHQsIG1hcyBlbnRlbmRlIG8gZG9jdW1lbnRvIGNvbW8gcGFydGUgZG8gYWNlcnZvIGludGVsZWN0dWFsIGRlc3NhIFVuaXZlcnNpZGFkZS4gCgogICAgUGFyYSBvcyBkb2N1bWVudG9zIHB1YmxpY2Fkb3MgY29tIHJlcGFzc2UgZGUgZGlyZWl0b3MgZGUgZGlzdHJpYnVp77+977+9bywgZXNzZSB0ZXJtbyBkZSBsaWNlbu+/vWEgZW50ZW5kZSBxdWU6IAoKICAgIE1hbnRlbmRvIG9zICBkaXJlaXRvcyBhdXRvcmFpcywgcmVwYXNzYWRvcyBhIHRlcmNlaXJvcywgZW0gY2FzbyAKZGUgcHVibGljYe+/ve+/vWVzLCBvIHJlcG9zaXTvv71yaW8gcG9kZSByZXN0cmluZ2lyIG8gYWNlc3NvIGFvIHRleHRvIAppbnRlZ3JhbCwgbWFzIGxpYmVyYSBhcyBpbmZvcm1h77+977+9ZXMgc29icmUgbyBkb2N1bWVudG8gKE1ldGFkYWRvcyBkZXNjcml0aXZvcykuCgogRGVzdGEgZm9ybWEsIGF0ZW5kZW5kbyBhb3MgYW5zZWlvcyBkZXNzYSB1bml2ZXJzaWRhZGUgCmVtIG1hbnRlciBzdWEgcHJvZHXvv73vv71vIGNpZW5077+9ZmljYSBjb20gYXMgcmVzdHJp77+977+9ZXMgaW1wb3N0YXMgcGVsb3MgCmVkaXRvcmVzIGRlIHBlcmnvv71kaWNvcy4gCgogICAgUGFyYSBhcyBwdWJsaWNh77+977+9ZXMgZW0gaW5pY2lhdGl2YXMgcXVlIHNlZ3VlbSBhIHBvbO+/vXRpY2EgZGUgCkFjZXNzbyBBYmVydG8sIG9zIGRlcO+/vXNpdG9zIGNvbXB1bHPvv71yaW9zIG5lc3NlIHJlcG9zaXTvv71yaW8gbWFudO+/vW0gCm9zIGRpcmVpdG9zIGF1dG9yYWlzLCBtYXMgbWFudO+/vW0gbyBhY2Vzc28gaXJyZXN0cml0byBhbyBtZXRhZGFkb3MgCmUgdGV4dG8gY29tcGxldG8uIEFzc2ltLCBhIGFjZWl0Ye+/ve+/vW8gZGVzc2UgdGVybW8gbu+/vW8gbmVjZXNzaXRhIGRlIApjb25zZW50aW1lbnRvIHBvciBwYXJ0ZSBkZSBhdXRvcmVzL2RldGVudG9yZXMgZG9zIGRpcmVpdG9zLCBwb3IgCmVzdGFyZW0gZW0gaW5pY2lhdGl2YXMgZGUgYWNlc3NvIGFiZXJ0by4KCiAgICBFbSBhbWJvcyBvIGNhc28sIGVzc2UgdGVybW8gZGUgbGljZW7vv71hLCBwb2RlIHNlciBhY2VpdG8gcGVsbyAKYXV0b3IsIGRldGVudG9yZXMgZGUgZGlyZWl0b3MgZS9vdSB0ZXJjZWlyb3MgYW1wYXJhZG9zIHBlbGEgCnVuaXZlcnNpZGFkZS4gRGV2aWRvIGFvcyBkaWZlcmVudGVzIHByb2Nlc3NvcyBwZWxvIHF1YWwgYSBzdWJtaXNz77+9byAKcG9kZSBvY29ycmVyLCBvIHJlcG9zaXTvv71yaW8gcGVybWl0ZSBhIGFjZWl0Ye+/ve+/vW8gZGEgbGljZW7vv71hIHBvciAKdGVyY2Vpcm9zLCBzb21lbnRlIG5vcyBjYXNvcyBkZSBkb2N1bWVudG9zIHByb2R1emlkb3MgcG9yIGludGVncmFudGVzIApkYSBVRkJBIGUgc3VibWV0aWRvcyBwb3IgcGVzc29hcyBhbXBhcmFkYXMgcG9yIGVzdGEgaW5zdGl0dWnvv73vv71vLgo=Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:04:02Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum
dc.title.alternative.pt_BR.fl_str_mv Analytical and Bioanalytical Chemistry
title A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum
spellingShingle A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum
Jesus, Joilson Ramos
Leishmania chagasi
Immunosensor
Self-assembled monolayer
Piezoelectric
title_short A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum
title_full A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum
title_fullStr A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum
title_full_unstemmed A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum
title_sort A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum
author Jesus, Joilson Ramos
author_facet Jesus, Joilson Ramos
Carvalho, Kellyanne dos Anjos
Fonseca, Rosana Anita da Silva
Oliveira, Geraldo Gileno de Sá
Melo, Stella Maria Barrouin
Neves, Neuza Maria Alcântara
Dutra, Rosa Amália Fireman
author_role author
author2 Carvalho, Kellyanne dos Anjos
Fonseca, Rosana Anita da Silva
Oliveira, Geraldo Gileno de Sá
Melo, Stella Maria Barrouin
Neves, Neuza Maria Alcântara
Dutra, Rosa Amália Fireman
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Jesus, Joilson Ramos
Carvalho, Kellyanne dos Anjos
Fonseca, Rosana Anita da Silva
Oliveira, Geraldo Gileno de Sá
Melo, Stella Maria Barrouin
Neves, Neuza Maria Alcântara
Dutra, Rosa Amália Fireman
Jesus, Joilson Ramos
Carvalho, Kellyanne dos Anjos
Fonseca, Rosana Anita da Silva
Oliveira, Geraldo Gileno de Sá
Melo, Stella Maria Barrouin
Neves, Neuza Maria Alcântara
Dutra, Rosa Amália Fireman
dc.subject.por.fl_str_mv Leishmania chagasi
Immunosensor
Self-assembled monolayer
Piezoelectric
topic Leishmania chagasi
Immunosensor
Self-assembled monolayer
Piezoelectric
description texto completo: restrito. p. 917–925
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2012-02-09T16:41:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/5366
dc.identifier.issn.none.fl_str_mv 1618-2650
dc.identifier.number.pt_BR.fl_str_mv v. 400.
identifier_str_mv 1618-2650
v. 400.
url http://www.repositorio.ufba.br/ri/handle/ri/5366
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv DOI 10.1007/s00216-011-5136-7
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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