Study of erythrocytes as a novel drug carrier for the delivery of artemether
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/164751 |
Resumo: | Resealed erythrocytes have been explored in various dimensions of drug delivery, owing to their high biocompatibility and inability to initiate immune response. The present research was designed to evaluate the drug delivery potential of erythrocytes by loading a hydrophobic anti-malarial drug, Artemether. Three different loading techniques were applied to achieve maximum optimized drug loading. A HPLC method was validated for drug quantification in erythrocytes. The relatively high loading was achieved using hypotonic treatment was 31.39% as compared to other two methods. These, drug loaded erythrocytes were characterized for membrane integrity via ESR showing higher ESR values for drug loaded cells as compared to normal cells. Moreover, microscopic evaluation was done to observe morphological changes in erythrocytes after successful loading which showed swollen cells with slight rough surface as compared to smooth surface of normal cells. Drug release was studied for 8 h which showed more than 80% release within 3-7 h from erythrocytes treated with different hypotonic methods. Overall, the study revealed a potential application of erythrocytes in delivery of hydrophobic drugs using hypotonic treatment as compared to other methods. |
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Brazilian Journal of Pharmaceutical Sciences |
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Study of erythrocytes as a novel drug carrier for the delivery of artemetherResealed ErythrocytesNovel Drug Delivery SystemHydophobic drug/artemetherResealed erythrocytes have been explored in various dimensions of drug delivery, owing to their high biocompatibility and inability to initiate immune response. The present research was designed to evaluate the drug delivery potential of erythrocytes by loading a hydrophobic anti-malarial drug, Artemether. Three different loading techniques were applied to achieve maximum optimized drug loading. A HPLC method was validated for drug quantification in erythrocytes. The relatively high loading was achieved using hypotonic treatment was 31.39% as compared to other two methods. These, drug loaded erythrocytes were characterized for membrane integrity via ESR showing higher ESR values for drug loaded cells as compared to normal cells. Moreover, microscopic evaluation was done to observe morphological changes in erythrocytes after successful loading which showed swollen cells with slight rough surface as compared to smooth surface of normal cells. Drug release was studied for 8 h which showed more than 80% release within 3-7 h from erythrocytes treated with different hypotonic methods. Overall, the study revealed a potential application of erythrocytes in delivery of hydrophobic drugs using hypotonic treatment as compared to other methods.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2019-12-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/16475110.1590/s2175-97902019000117680Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e17680Brazilian Journal of Pharmaceutical Sciences; v. 55 (2019); e17680Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e176802175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/164751/157943Copyright (c) 2019 Brazilian Journal of Pharmaceutical Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRiaz, Muhammad IjazSarwar, Hafiz ShoaibRehman, MubashirGohar, Umar FarooqRaza, Syed AtifSiddique, Muhammad IrfanShahnaz, GulSohail, Muhammadl Farhan2021-01-11T18:12:48Zoai:revistas.usp.br:article/164751Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2021-01-11T18:12:48Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Study of erythrocytes as a novel drug carrier for the delivery of artemether |
title |
Study of erythrocytes as a novel drug carrier for the delivery of artemether |
spellingShingle |
Study of erythrocytes as a novel drug carrier for the delivery of artemether Riaz, Muhammad Ijaz Resealed Erythrocytes Novel Drug Delivery System Hydophobic drug/artemether |
title_short |
Study of erythrocytes as a novel drug carrier for the delivery of artemether |
title_full |
Study of erythrocytes as a novel drug carrier for the delivery of artemether |
title_fullStr |
Study of erythrocytes as a novel drug carrier for the delivery of artemether |
title_full_unstemmed |
Study of erythrocytes as a novel drug carrier for the delivery of artemether |
title_sort |
Study of erythrocytes as a novel drug carrier for the delivery of artemether |
author |
Riaz, Muhammad Ijaz |
author_facet |
Riaz, Muhammad Ijaz Sarwar, Hafiz Shoaib Rehman, Mubashir Gohar, Umar Farooq Raza, Syed Atif Siddique, Muhammad Irfan Shahnaz, Gul Sohail, Muhammadl Farhan |
author_role |
author |
author2 |
Sarwar, Hafiz Shoaib Rehman, Mubashir Gohar, Umar Farooq Raza, Syed Atif Siddique, Muhammad Irfan Shahnaz, Gul Sohail, Muhammadl Farhan |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Riaz, Muhammad Ijaz Sarwar, Hafiz Shoaib Rehman, Mubashir Gohar, Umar Farooq Raza, Syed Atif Siddique, Muhammad Irfan Shahnaz, Gul Sohail, Muhammadl Farhan |
dc.subject.por.fl_str_mv |
Resealed Erythrocytes Novel Drug Delivery System Hydophobic drug/artemether |
topic |
Resealed Erythrocytes Novel Drug Delivery System Hydophobic drug/artemether |
description |
Resealed erythrocytes have been explored in various dimensions of drug delivery, owing to their high biocompatibility and inability to initiate immune response. The present research was designed to evaluate the drug delivery potential of erythrocytes by loading a hydrophobic anti-malarial drug, Artemether. Three different loading techniques were applied to achieve maximum optimized drug loading. A HPLC method was validated for drug quantification in erythrocytes. The relatively high loading was achieved using hypotonic treatment was 31.39% as compared to other two methods. These, drug loaded erythrocytes were characterized for membrane integrity via ESR showing higher ESR values for drug loaded cells as compared to normal cells. Moreover, microscopic evaluation was done to observe morphological changes in erythrocytes after successful loading which showed swollen cells with slight rough surface as compared to smooth surface of normal cells. Drug release was studied for 8 h which showed more than 80% release within 3-7 h from erythrocytes treated with different hypotonic methods. Overall, the study revealed a potential application of erythrocytes in delivery of hydrophobic drugs using hypotonic treatment as compared to other methods. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-03 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/164751 10.1590/s2175-97902019000117680 |
url |
https://www.revistas.usp.br/bjps/article/view/164751 |
identifier_str_mv |
10.1590/s2175-97902019000117680 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/164751/157943 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Brazilian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Brazilian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e17680 Brazilian Journal of Pharmaceutical Sciences; v. 55 (2019); e17680 Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e17680 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1800222914477293568 |