Evaluation of chromatin integrity of fowl spermatozoa (Gallus gallus, Linnaeus, 1758) from meat chicken in two ages

Detalhes bibliográficos
Autor(a) principal: Soares, Jaqueline Melo
Data de Publicação: 2006
Outros Autores: Beletti, Marcelo Emílio
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/26471
Resumo: In poultry breeding, male fertility has extreme importance to assure a better production of fertile eggs. Evaluation techniques for fertility of fowls are not well explored and in most cases, the evaluation is done through sampling considering only morphophysiological factors directly related with the spermatozoon. It's known that in other species, besides morphophysiological factors, there are intrinsic problems to the spermatozoon such as less chromatin condensation that can lead to fertility problems that in most cases are not diagnosised. The purpose of this work was the adaptation of techniques of chromatin evaluation for chicken (Gallus Gallus, Linnaeus, 1758) which have already been described in other species, correlating the chromatin alterations with the morphological alterations and the fertility. Fowl semen samples with different levels of fertility were used for test different methods to identify chromatin alterations using toluidine blue and acridine orange die. Evaluations have shown that smears of fresh semen with posterior fixation generate artfacts that change the head shape and the chromatin integrity not being indicated in methods of fertility evaluation. Although all the tested methods have presented methodology imperfections and some certain degree of subjectivity, the method that has generated best results was the mixture of one semen drop conserved in buffered phormol and a drop of acridine orange on microscopy slide with posterior staining and observation in fluorescence microscopy with blue excitation filter. Through this method it was verified that alterations in the chromatin condensation of fowl spermatozoa are generally not followed by morphological alterations and that generally the morphologic alterations of fowl spermatozoa are generally followed by alterations in chromatin condensation. This is an important factor in the determination of the fowl fertility.
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spelling Evaluation of chromatin integrity of fowl spermatozoa (Gallus gallus, Linnaeus, 1758) from meat chicken in two agesAvaliação da integridade cromatínica de espermatozóides de galos (Gallus gallus, Linnaeus, 1758) de linhagem pesada em duas idadesGaloEspermatozóideCompactação de cromatinaAzul de toluidinaAlaranjado de acridinaFowlSpermatozoonChromatin condensationToluidine blueAcridine orangeIn poultry breeding, male fertility has extreme importance to assure a better production of fertile eggs. Evaluation techniques for fertility of fowls are not well explored and in most cases, the evaluation is done through sampling considering only morphophysiological factors directly related with the spermatozoon. It's known that in other species, besides morphophysiological factors, there are intrinsic problems to the spermatozoon such as less chromatin condensation that can lead to fertility problems that in most cases are not diagnosised. The purpose of this work was the adaptation of techniques of chromatin evaluation for chicken (Gallus Gallus, Linnaeus, 1758) which have already been described in other species, correlating the chromatin alterations with the morphological alterations and the fertility. Fowl semen samples with different levels of fertility were used for test different methods to identify chromatin alterations using toluidine blue and acridine orange die. Evaluations have shown that smears of fresh semen with posterior fixation generate artfacts that change the head shape and the chromatin integrity not being indicated in methods of fertility evaluation. Although all the tested methods have presented methodology imperfections and some certain degree of subjectivity, the method that has generated best results was the mixture of one semen drop conserved in buffered phormol and a drop of acridine orange on microscopy slide with posterior staining and observation in fluorescence microscopy with blue excitation filter. Through this method it was verified that alterations in the chromatin condensation of fowl spermatozoa are generally not followed by morphological alterations and that generally the morphologic alterations of fowl spermatozoa are generally followed by alterations in chromatin condensation. This is an important factor in the determination of the fowl fertility.Na avicultura, a avaliação de fertilidade em machos é de extrema importância, para garantir uma melhor produção de ovos férteis. As técnicas para avaliação de fertilidade em galos são pouco exploradas, sendo que na maioria das vezes, a avaliação é feita por amostragem e levando em consideração, apenas fatores morfofisiológicos, diretamente relacionados com o espermatozóide. Mas é sabido que, em outras espécies, além dos fatores morfofisiológicos, existem problemas intrínsecos ao espermatozóide, como a baixa compactação da cromatina, que pode levar a disturbios de fertilidade, que na maioria das vezes não são diagnosticados. O objetivo desse trabalho foi a adaptação de técnicas de avaliação da cromatina, já descritas em outras espécies, para aves de linhagem pesada (Gallus gallus, Linnaeus, 1758), correlacionando as alterações cromatínicas com as alterações morfológicas e com a fertilidade. Para tanto, sêmen de galo com diferentes níveis de fertilidade, foram utilizados em diferentes métodos para identificação de alterações na cromatina, utilizando os corantes azul de toluidina e alaranjado de acridina. As avaliações demonstraram que esfregaços de sêmen fresco de galo com posterior fixação geram artefatos que levam a alterações na forma da cabeça e na integridade da cromatina, não sendo indicados em métodos de avaliação de fertilidade. Apesar de todos os métodos testados apresentarem falhas metodológicas e um certo grau de subjetividade, o método que gerou melhores resultados foi a mistura de uma gota de sêmen conservado em formol salina e uma gota do alaranjado de acridina sobre lâmina de microscopia, com posterior secagem e observação em microscopia de fluorescência com filtro de excitação azul. Pelo método verificou-se que alterações na compactação da cromatina de espermatozóides de galo geralmente não são acompanhadas por alterações morfológicas e que geralmente as alterações morfológicas de espermatozóides de galo são acompanhadas por alterações na compactação da cromatina e que esse é um importante fator na determinação da fertilidade de galos.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2006-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/2647110.11606/issn.1678-4456.bjvras.2006.26471Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 Núm. 4 (2006); 543-553Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 No. 4 (2006); 543-553Brazilian Journal of Veterinary Research and Animal Science; v. 43 n. 4 (2006); 543-553Brazilian Journal of Veterinary Research and Animal Science; V. 43 N. 4 (2006); 543-5531678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/26471/28254Soares, Jaqueline MeloBeletti, Marcelo Emílioinfo:eu-repo/semantics/openAccess2020-06-23T04:17:18Zoai:revistas.usp.br:article/26471Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:45.238561Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Evaluation of chromatin integrity of fowl spermatozoa (Gallus gallus, Linnaeus, 1758) from meat chicken in two ages
Avaliação da integridade cromatínica de espermatozóides de galos (Gallus gallus, Linnaeus, 1758) de linhagem pesada em duas idades
title Evaluation of chromatin integrity of fowl spermatozoa (Gallus gallus, Linnaeus, 1758) from meat chicken in two ages
spellingShingle Evaluation of chromatin integrity of fowl spermatozoa (Gallus gallus, Linnaeus, 1758) from meat chicken in two ages
Soares, Jaqueline Melo
Galo
Espermatozóide
Compactação de cromatina
Azul de toluidina
Alaranjado de acridina
Fowl
Spermatozoon
Chromatin condensation
Toluidine blue
Acridine orange
title_short Evaluation of chromatin integrity of fowl spermatozoa (Gallus gallus, Linnaeus, 1758) from meat chicken in two ages
title_full Evaluation of chromatin integrity of fowl spermatozoa (Gallus gallus, Linnaeus, 1758) from meat chicken in two ages
title_fullStr Evaluation of chromatin integrity of fowl spermatozoa (Gallus gallus, Linnaeus, 1758) from meat chicken in two ages
title_full_unstemmed Evaluation of chromatin integrity of fowl spermatozoa (Gallus gallus, Linnaeus, 1758) from meat chicken in two ages
title_sort Evaluation of chromatin integrity of fowl spermatozoa (Gallus gallus, Linnaeus, 1758) from meat chicken in two ages
author Soares, Jaqueline Melo
author_facet Soares, Jaqueline Melo
Beletti, Marcelo Emílio
author_role author
author2 Beletti, Marcelo Emílio
author2_role author
dc.contributor.author.fl_str_mv Soares, Jaqueline Melo
Beletti, Marcelo Emílio
dc.subject.por.fl_str_mv Galo
Espermatozóide
Compactação de cromatina
Azul de toluidina
Alaranjado de acridina
Fowl
Spermatozoon
Chromatin condensation
Toluidine blue
Acridine orange
topic Galo
Espermatozóide
Compactação de cromatina
Azul de toluidina
Alaranjado de acridina
Fowl
Spermatozoon
Chromatin condensation
Toluidine blue
Acridine orange
description In poultry breeding, male fertility has extreme importance to assure a better production of fertile eggs. Evaluation techniques for fertility of fowls are not well explored and in most cases, the evaluation is done through sampling considering only morphophysiological factors directly related with the spermatozoon. It's known that in other species, besides morphophysiological factors, there are intrinsic problems to the spermatozoon such as less chromatin condensation that can lead to fertility problems that in most cases are not diagnosised. The purpose of this work was the adaptation of techniques of chromatin evaluation for chicken (Gallus Gallus, Linnaeus, 1758) which have already been described in other species, correlating the chromatin alterations with the morphological alterations and the fertility. Fowl semen samples with different levels of fertility were used for test different methods to identify chromatin alterations using toluidine blue and acridine orange die. Evaluations have shown that smears of fresh semen with posterior fixation generate artfacts that change the head shape and the chromatin integrity not being indicated in methods of fertility evaluation. Although all the tested methods have presented methodology imperfections and some certain degree of subjectivity, the method that has generated best results was the mixture of one semen drop conserved in buffered phormol and a drop of acridine orange on microscopy slide with posterior staining and observation in fluorescence microscopy with blue excitation filter. Through this method it was verified that alterations in the chromatin condensation of fowl spermatozoa are generally not followed by morphological alterations and that generally the morphologic alterations of fowl spermatozoa are generally followed by alterations in chromatin condensation. This is an important factor in the determination of the fowl fertility.
publishDate 2006
dc.date.none.fl_str_mv 2006-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/26471
10.11606/issn.1678-4456.bjvras.2006.26471
url https://www.revistas.usp.br/bjvras/article/view/26471
identifier_str_mv 10.11606/issn.1678-4456.bjvras.2006.26471
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/26471/28254
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 Núm. 4 (2006); 543-553
Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 No. 4 (2006); 543-553
Brazilian Journal of Veterinary Research and Animal Science; v. 43 n. 4 (2006); 543-553
Brazilian Journal of Veterinary Research and Animal Science; V. 43 N. 4 (2006); 543-553
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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