Evaluation of chromatin integrity of fowl spermatozoa (Gallus gallus, Linnaeus, 1758) from meat chicken in two ages
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/26471 |
Resumo: | In poultry breeding, male fertility has extreme importance to assure a better production of fertile eggs. Evaluation techniques for fertility of fowls are not well explored and in most cases, the evaluation is done through sampling considering only morphophysiological factors directly related with the spermatozoon. It's known that in other species, besides morphophysiological factors, there are intrinsic problems to the spermatozoon such as less chromatin condensation that can lead to fertility problems that in most cases are not diagnosised. The purpose of this work was the adaptation of techniques of chromatin evaluation for chicken (Gallus Gallus, Linnaeus, 1758) which have already been described in other species, correlating the chromatin alterations with the morphological alterations and the fertility. Fowl semen samples with different levels of fertility were used for test different methods to identify chromatin alterations using toluidine blue and acridine orange die. Evaluations have shown that smears of fresh semen with posterior fixation generate artfacts that change the head shape and the chromatin integrity not being indicated in methods of fertility evaluation. Although all the tested methods have presented methodology imperfections and some certain degree of subjectivity, the method that has generated best results was the mixture of one semen drop conserved in buffered phormol and a drop of acridine orange on microscopy slide with posterior staining and observation in fluorescence microscopy with blue excitation filter. Through this method it was verified that alterations in the chromatin condensation of fowl spermatozoa are generally not followed by morphological alterations and that generally the morphologic alterations of fowl spermatozoa are generally followed by alterations in chromatin condensation. This is an important factor in the determination of the fowl fertility. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Evaluation of chromatin integrity of fowl spermatozoa (Gallus gallus, Linnaeus, 1758) from meat chicken in two agesAvaliação da integridade cromatínica de espermatozóides de galos (Gallus gallus, Linnaeus, 1758) de linhagem pesada em duas idadesGaloEspermatozóideCompactação de cromatinaAzul de toluidinaAlaranjado de acridinaFowlSpermatozoonChromatin condensationToluidine blueAcridine orangeIn poultry breeding, male fertility has extreme importance to assure a better production of fertile eggs. Evaluation techniques for fertility of fowls are not well explored and in most cases, the evaluation is done through sampling considering only morphophysiological factors directly related with the spermatozoon. It's known that in other species, besides morphophysiological factors, there are intrinsic problems to the spermatozoon such as less chromatin condensation that can lead to fertility problems that in most cases are not diagnosised. The purpose of this work was the adaptation of techniques of chromatin evaluation for chicken (Gallus Gallus, Linnaeus, 1758) which have already been described in other species, correlating the chromatin alterations with the morphological alterations and the fertility. Fowl semen samples with different levels of fertility were used for test different methods to identify chromatin alterations using toluidine blue and acridine orange die. Evaluations have shown that smears of fresh semen with posterior fixation generate artfacts that change the head shape and the chromatin integrity not being indicated in methods of fertility evaluation. Although all the tested methods have presented methodology imperfections and some certain degree of subjectivity, the method that has generated best results was the mixture of one semen drop conserved in buffered phormol and a drop of acridine orange on microscopy slide with posterior staining and observation in fluorescence microscopy with blue excitation filter. Through this method it was verified that alterations in the chromatin condensation of fowl spermatozoa are generally not followed by morphological alterations and that generally the morphologic alterations of fowl spermatozoa are generally followed by alterations in chromatin condensation. This is an important factor in the determination of the fowl fertility.Na avicultura, a avaliação de fertilidade em machos é de extrema importância, para garantir uma melhor produção de ovos férteis. As técnicas para avaliação de fertilidade em galos são pouco exploradas, sendo que na maioria das vezes, a avaliação é feita por amostragem e levando em consideração, apenas fatores morfofisiológicos, diretamente relacionados com o espermatozóide. Mas é sabido que, em outras espécies, além dos fatores morfofisiológicos, existem problemas intrínsecos ao espermatozóide, como a baixa compactação da cromatina, que pode levar a disturbios de fertilidade, que na maioria das vezes não são diagnosticados. O objetivo desse trabalho foi a adaptação de técnicas de avaliação da cromatina, já descritas em outras espécies, para aves de linhagem pesada (Gallus gallus, Linnaeus, 1758), correlacionando as alterações cromatínicas com as alterações morfológicas e com a fertilidade. Para tanto, sêmen de galo com diferentes níveis de fertilidade, foram utilizados em diferentes métodos para identificação de alterações na cromatina, utilizando os corantes azul de toluidina e alaranjado de acridina. As avaliações demonstraram que esfregaços de sêmen fresco de galo com posterior fixação geram artefatos que levam a alterações na forma da cabeça e na integridade da cromatina, não sendo indicados em métodos de avaliação de fertilidade. Apesar de todos os métodos testados apresentarem falhas metodológicas e um certo grau de subjetividade, o método que gerou melhores resultados foi a mistura de uma gota de sêmen conservado em formol salina e uma gota do alaranjado de acridina sobre lâmina de microscopia, com posterior secagem e observação em microscopia de fluorescência com filtro de excitação azul. Pelo método verificou-se que alterações na compactação da cromatina de espermatozóides de galo geralmente não são acompanhadas por alterações morfológicas e que geralmente as alterações morfológicas de espermatozóides de galo são acompanhadas por alterações na compactação da cromatina e que esse é um importante fator na determinação da fertilidade de galos.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2006-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/2647110.11606/issn.1678-4456.bjvras.2006.26471Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 Núm. 4 (2006); 543-553Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 No. 4 (2006); 543-553Brazilian Journal of Veterinary Research and Animal Science; v. 43 n. 4 (2006); 543-553Brazilian Journal of Veterinary Research and Animal Science; V. 43 N. 4 (2006); 543-5531678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/26471/28254Soares, Jaqueline MeloBeletti, Marcelo Emílioinfo:eu-repo/semantics/openAccess2020-06-23T04:17:18Zoai:revistas.usp.br:article/26471Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:45.238561Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Evaluation of chromatin integrity of fowl spermatozoa (Gallus gallus, Linnaeus, 1758) from meat chicken in two ages Avaliação da integridade cromatínica de espermatozóides de galos (Gallus gallus, Linnaeus, 1758) de linhagem pesada em duas idades |
title |
Evaluation of chromatin integrity of fowl spermatozoa (Gallus gallus, Linnaeus, 1758) from meat chicken in two ages |
spellingShingle |
Evaluation of chromatin integrity of fowl spermatozoa (Gallus gallus, Linnaeus, 1758) from meat chicken in two ages Soares, Jaqueline Melo Galo Espermatozóide Compactação de cromatina Azul de toluidina Alaranjado de acridina Fowl Spermatozoon Chromatin condensation Toluidine blue Acridine orange |
title_short |
Evaluation of chromatin integrity of fowl spermatozoa (Gallus gallus, Linnaeus, 1758) from meat chicken in two ages |
title_full |
Evaluation of chromatin integrity of fowl spermatozoa (Gallus gallus, Linnaeus, 1758) from meat chicken in two ages |
title_fullStr |
Evaluation of chromatin integrity of fowl spermatozoa (Gallus gallus, Linnaeus, 1758) from meat chicken in two ages |
title_full_unstemmed |
Evaluation of chromatin integrity of fowl spermatozoa (Gallus gallus, Linnaeus, 1758) from meat chicken in two ages |
title_sort |
Evaluation of chromatin integrity of fowl spermatozoa (Gallus gallus, Linnaeus, 1758) from meat chicken in two ages |
author |
Soares, Jaqueline Melo |
author_facet |
Soares, Jaqueline Melo Beletti, Marcelo Emílio |
author_role |
author |
author2 |
Beletti, Marcelo Emílio |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Soares, Jaqueline Melo Beletti, Marcelo Emílio |
dc.subject.por.fl_str_mv |
Galo Espermatozóide Compactação de cromatina Azul de toluidina Alaranjado de acridina Fowl Spermatozoon Chromatin condensation Toluidine blue Acridine orange |
topic |
Galo Espermatozóide Compactação de cromatina Azul de toluidina Alaranjado de acridina Fowl Spermatozoon Chromatin condensation Toluidine blue Acridine orange |
description |
In poultry breeding, male fertility has extreme importance to assure a better production of fertile eggs. Evaluation techniques for fertility of fowls are not well explored and in most cases, the evaluation is done through sampling considering only morphophysiological factors directly related with the spermatozoon. It's known that in other species, besides morphophysiological factors, there are intrinsic problems to the spermatozoon such as less chromatin condensation that can lead to fertility problems that in most cases are not diagnosised. The purpose of this work was the adaptation of techniques of chromatin evaluation for chicken (Gallus Gallus, Linnaeus, 1758) which have already been described in other species, correlating the chromatin alterations with the morphological alterations and the fertility. Fowl semen samples with different levels of fertility were used for test different methods to identify chromatin alterations using toluidine blue and acridine orange die. Evaluations have shown that smears of fresh semen with posterior fixation generate artfacts that change the head shape and the chromatin integrity not being indicated in methods of fertility evaluation. Although all the tested methods have presented methodology imperfections and some certain degree of subjectivity, the method that has generated best results was the mixture of one semen drop conserved in buffered phormol and a drop of acridine orange on microscopy slide with posterior staining and observation in fluorescence microscopy with blue excitation filter. Through this method it was verified that alterations in the chromatin condensation of fowl spermatozoa are generally not followed by morphological alterations and that generally the morphologic alterations of fowl spermatozoa are generally followed by alterations in chromatin condensation. This is an important factor in the determination of the fowl fertility. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26471 10.11606/issn.1678-4456.bjvras.2006.26471 |
url |
https://www.revistas.usp.br/bjvras/article/view/26471 |
identifier_str_mv |
10.11606/issn.1678-4456.bjvras.2006.26471 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26471/28254 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 Núm. 4 (2006); 543-553 Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 No. 4 (2006); 543-553 Brazilian Journal of Veterinary Research and Animal Science; v. 43 n. 4 (2006); 543-553 Brazilian Journal of Veterinary Research and Animal Science; V. 43 N. 4 (2006); 543-553 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051557674483712 |