Morphology and chromatin condensation evaluation in fowl spermatozoa (Gallus gallus, Linnaeus, 1758) through the transmission electron microscopy
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
DOI: | 10.11606/issn.1678-4456.bjvras.2006.26472 |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/26472 |
Resumo: | The spermiogram is one of the principal methods of evaluation of the fertility in mammals; however some semen modifications as the spermatozoa chromatin condensation, are not identified in this routine, and can expressively interfere in the fertility of reproducers. In poultry breeding, generally the evaluation of reproducers is made by sampling and the evaluated parameters are in fewer quantities than in mammals, considering the spermatozoa chromatin condensation of the fowl has never been exploited. The objective of this work is verifying through the transmission electron microscopy, whether there are different intensities of spermatozoa chromatin condensation of fowl. So that, have been collected 20 samples of fowl semen, which were placed in 4% glutaraldehyde buffered in 0.1M sodium cacodilate to pH 7.2 for 48 hours, after being centrifugated and rinsed in cacodilate tampon, the semen was post-fixed in 1% osmium tetroxide plus 1.25% potassium ferrocyanide. The sediment was embedded, cut and contrasted. The thin sections were examined and documented in transmission electronic microscopy Zeiss EM-109. Generally the fowl spermatozoon has acrosome with homogenic or slightly granular and of moderate density material, the nucleus with dense or slightly granular chromatin, varies the gray scales, that is, the grades of condensation. It was also observed the "perforatorium", structure that links the nucleus to the acrosome. The insertion of the tail is made through two cetriules, being one transversal and the other longitudinal to the axle. The intermediate piece has typical axoneme involved by mitochondrias, apparently displayed longitudinally. The transition between the principal and the intermediate pieces of the tail is marked by the absence of the mitochondrias. The principal piece is formed by axoneme involved by a granular cytoplasm layer. In the evaluation of the morphological pathologies, it was observed rounded heads, folded, involved by the intermediate container of the piece, in a hook and all of them showing different grades of condensation. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Morphology and chromatin condensation evaluation in fowl spermatozoa (Gallus gallus, Linnaeus, 1758) through the transmission electron microscopyAvaliação da morfologia e da compactação cromatínica em espermatozóides de galo (Gallus gallus, Linnaeus, 1758) através de microscopia eletrônica de transmissãoGaloEspermatozóideCompactação de cromatinaFertilidadeUltra-estruturaFowlSpermatozoonChromatin condensationFertilityUltrastructureThe spermiogram is one of the principal methods of evaluation of the fertility in mammals; however some semen modifications as the spermatozoa chromatin condensation, are not identified in this routine, and can expressively interfere in the fertility of reproducers. In poultry breeding, generally the evaluation of reproducers is made by sampling and the evaluated parameters are in fewer quantities than in mammals, considering the spermatozoa chromatin condensation of the fowl has never been exploited. The objective of this work is verifying through the transmission electron microscopy, whether there are different intensities of spermatozoa chromatin condensation of fowl. So that, have been collected 20 samples of fowl semen, which were placed in 4% glutaraldehyde buffered in 0.1M sodium cacodilate to pH 7.2 for 48 hours, after being centrifugated and rinsed in cacodilate tampon, the semen was post-fixed in 1% osmium tetroxide plus 1.25% potassium ferrocyanide. The sediment was embedded, cut and contrasted. The thin sections were examined and documented in transmission electronic microscopy Zeiss EM-109. Generally the fowl spermatozoon has acrosome with homogenic or slightly granular and of moderate density material, the nucleus with dense or slightly granular chromatin, varies the gray scales, that is, the grades of condensation. It was also observed the "perforatorium", structure that links the nucleus to the acrosome. The insertion of the tail is made through two cetriules, being one transversal and the other longitudinal to the axle. The intermediate piece has typical axoneme involved by mitochondrias, apparently displayed longitudinally. The transition between the principal and the intermediate pieces of the tail is marked by the absence of the mitochondrias. The principal piece is formed by axoneme involved by a granular cytoplasm layer. In the evaluation of the morphological pathologies, it was observed rounded heads, folded, involved by the intermediate container of the piece, in a hook and all of them showing different grades of condensation.O espermograma é um dos principais métodos de avaliação da fertilidade em mamíferos, contudo, algumas alterações do sêmen como a compactação da cromatina dos espermatozóides, não são identificadas nessa rotina, podendo interferir significativamente na fertilidade de reprodutores. Na avicultura, geralmente a avaliação de reprodutores é feita por amostragem e os parâmetros avaliados são em menor número que em mamíferos, sendo que a compactação da cromatina dos espermatozóides de galo nunca foi explorada. Este trabalho teve como objetivo verificar através da microscopia eletrônica de transmissão, se existe diferentes intensidades de compactação de cromatina em espermatozóides de galo. Para isto, foram coletadas 20 amostras de sêmen de galo, que foram fixadas por 48 horas em glutaraldeído 4% tamponado em cacodilato de sódio 0,1M a pH 7,2, após centrifugado e lavado em tampão cacodilato o sêmen foi pós-fixados em tetróxido de ósmio a 1% mais ferrocianeto de potássio a 1,25%. O sedimento foi incluído, cortado e posteriormente contrastado. Os cortes foram examinados e documentados em microscópio eletrônico Zeiss EM-109. Geralmente, os espermatozóides de galo possuem acrossoma com material homogêneo ou levemente granular e de densidade moderada, o núcleo com cromatina densa e levemente granular, varia as tonalidades de cinza, ou seja, os graus de compactação. Observou-se também o "perforatorium", estrutura que une o núcleo ao acrossoma. A inserção da cauda é por meio de dois centríolos, sendo um transversal e o outro longitudinal ao eixo. A peça intermediária possui axonema típico envolto por mitocôndrias aparentemente dispostas longitudinalmente. A transição entre as peças intermediária e principal da cauda é marcada pela ausência das mitocôndrias. A peça principal é formada pelo axonema envolto por uma camada de citoplasma granular. Na avaliação das patologias morfológicas observaram-se cabeças arredondadas, dobradas, envoltas por conteúdo da peça intermediária, em anzol e todas apresentando diferentes graus de compactação.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2006-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/2647210.11606/issn.1678-4456.bjvras.2006.26472Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 Núm. 4 (2006); 554-560Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 No. 4 (2006); 554-560Brazilian Journal of Veterinary Research and Animal Science; v. 43 n. 4 (2006); 554-560Brazilian Journal of Veterinary Research and Animal Science; V. 43 N. 4 (2006); 554-5601678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/26472/28255Soares, Jaqueline MeloBeletti, Marcelo Emílioinfo:eu-repo/semantics/openAccess2020-06-23T04:17:17Zoai:revistas.usp.br:article/26472Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:45.300319Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Morphology and chromatin condensation evaluation in fowl spermatozoa (Gallus gallus, Linnaeus, 1758) through the transmission electron microscopy Avaliação da morfologia e da compactação cromatínica em espermatozóides de galo (Gallus gallus, Linnaeus, 1758) através de microscopia eletrônica de transmissão |
title |
Morphology and chromatin condensation evaluation in fowl spermatozoa (Gallus gallus, Linnaeus, 1758) through the transmission electron microscopy |
spellingShingle |
Morphology and chromatin condensation evaluation in fowl spermatozoa (Gallus gallus, Linnaeus, 1758) through the transmission electron microscopy Morphology and chromatin condensation evaluation in fowl spermatozoa (Gallus gallus, Linnaeus, 1758) through the transmission electron microscopy Soares, Jaqueline Melo Galo Espermatozóide Compactação de cromatina Fertilidade Ultra-estrutura Fowl Spermatozoon Chromatin condensation Fertility Ultrastructure Soares, Jaqueline Melo Galo Espermatozóide Compactação de cromatina Fertilidade Ultra-estrutura Fowl Spermatozoon Chromatin condensation Fertility Ultrastructure |
title_short |
Morphology and chromatin condensation evaluation in fowl spermatozoa (Gallus gallus, Linnaeus, 1758) through the transmission electron microscopy |
title_full |
Morphology and chromatin condensation evaluation in fowl spermatozoa (Gallus gallus, Linnaeus, 1758) through the transmission electron microscopy |
title_fullStr |
Morphology and chromatin condensation evaluation in fowl spermatozoa (Gallus gallus, Linnaeus, 1758) through the transmission electron microscopy Morphology and chromatin condensation evaluation in fowl spermatozoa (Gallus gallus, Linnaeus, 1758) through the transmission electron microscopy |
title_full_unstemmed |
Morphology and chromatin condensation evaluation in fowl spermatozoa (Gallus gallus, Linnaeus, 1758) through the transmission electron microscopy Morphology and chromatin condensation evaluation in fowl spermatozoa (Gallus gallus, Linnaeus, 1758) through the transmission electron microscopy |
title_sort |
Morphology and chromatin condensation evaluation in fowl spermatozoa (Gallus gallus, Linnaeus, 1758) through the transmission electron microscopy |
author |
Soares, Jaqueline Melo |
author_facet |
Soares, Jaqueline Melo Soares, Jaqueline Melo Beletti, Marcelo Emílio Beletti, Marcelo Emílio |
author_role |
author |
author2 |
Beletti, Marcelo Emílio |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Soares, Jaqueline Melo Beletti, Marcelo Emílio |
dc.subject.por.fl_str_mv |
Galo Espermatozóide Compactação de cromatina Fertilidade Ultra-estrutura Fowl Spermatozoon Chromatin condensation Fertility Ultrastructure |
topic |
Galo Espermatozóide Compactação de cromatina Fertilidade Ultra-estrutura Fowl Spermatozoon Chromatin condensation Fertility Ultrastructure |
description |
The spermiogram is one of the principal methods of evaluation of the fertility in mammals; however some semen modifications as the spermatozoa chromatin condensation, are not identified in this routine, and can expressively interfere in the fertility of reproducers. In poultry breeding, generally the evaluation of reproducers is made by sampling and the evaluated parameters are in fewer quantities than in mammals, considering the spermatozoa chromatin condensation of the fowl has never been exploited. The objective of this work is verifying through the transmission electron microscopy, whether there are different intensities of spermatozoa chromatin condensation of fowl. So that, have been collected 20 samples of fowl semen, which were placed in 4% glutaraldehyde buffered in 0.1M sodium cacodilate to pH 7.2 for 48 hours, after being centrifugated and rinsed in cacodilate tampon, the semen was post-fixed in 1% osmium tetroxide plus 1.25% potassium ferrocyanide. The sediment was embedded, cut and contrasted. The thin sections were examined and documented in transmission electronic microscopy Zeiss EM-109. Generally the fowl spermatozoon has acrosome with homogenic or slightly granular and of moderate density material, the nucleus with dense or slightly granular chromatin, varies the gray scales, that is, the grades of condensation. It was also observed the "perforatorium", structure that links the nucleus to the acrosome. The insertion of the tail is made through two cetriules, being one transversal and the other longitudinal to the axle. The intermediate piece has typical axoneme involved by mitochondrias, apparently displayed longitudinally. The transition between the principal and the intermediate pieces of the tail is marked by the absence of the mitochondrias. The principal piece is formed by axoneme involved by a granular cytoplasm layer. In the evaluation of the morphological pathologies, it was observed rounded heads, folded, involved by the intermediate container of the piece, in a hook and all of them showing different grades of condensation. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26472 10.11606/issn.1678-4456.bjvras.2006.26472 |
url |
https://www.revistas.usp.br/bjvras/article/view/26472 |
identifier_str_mv |
10.11606/issn.1678-4456.bjvras.2006.26472 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26472/28255 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 Núm. 4 (2006); 554-560 Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 No. 4 (2006); 554-560 Brazilian Journal of Veterinary Research and Animal Science; v. 43 n. 4 (2006); 554-560 Brazilian Journal of Veterinary Research and Animal Science; V. 43 N. 4 (2006); 554-560 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
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1822182143815581696 |
dc.identifier.doi.none.fl_str_mv |
10.11606/issn.1678-4456.bjvras.2006.26472 |