Can centrifugation force compromise the plasmatic membrane, acrosome and DNA integrity of goat spermatozoa?
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/145873 |
Resumo: | Protocols for cooling or freezing goat semen usually recommend centrifugation for seminal plasma removal. However, little is known about the effect of this process on goat sperm viability and functionality. The present study evaluated the effects of centrifugation force on the plasma membrane, acrosomes, and DNA integrity of goat semen. Four ejaculates from each of the four different Anglo Nubian male goats were used. Semen samples were obtained using artificial vagina, and immediately after collection, ejaculates were diluted using Ringer’s sodium lactate solution and split into three groups: Control (CG, without centrifugation), G1 (centrifugation 600 x g/10 min), G2 (centrifugation 1200 x g/10 min). After centrifugation, seminal plasma was removed, the sperm pellets were resuspended using Tris-egg yolk extender (80 x 106 spermatozoa/mL) and the sperm morphology was analyzed. Samples were cooled at 5°C for 5, 24, 36, and 48 h and then sperm plasma membrane and acrosome integrity (PMAI, %) and sperm DNA fragmentation index (SDF, %) were evaluated at each time-point, using a flow cytometer. Additionally, sperm movement was determined using computer semen analysis (CASA) after 5, 24, and 48 h of refrigeration period. The semen centrifugation did not induce additional sperm morphology defect or reduction in sperm kinetics in the experimental groups. Differences were not observed (p > 0.05) in PMAI and SDF among different groups, in any of each timepointof the cooling process. In conclusion, centrifugation, even at high speeds, did not affect goat sperm integrity and functionality when submitted to refrigeration process. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Can centrifugation force compromise the plasmatic membrane, acrosome and DNA integrity of goat spermatozoa?A força de centrifugação pode comprometer a integridade de membrana plasmática, acrossomal e DNA de espermatozoides caprinos?CentrifugationGoatIntegritySemenViabilityCaprinoCentrifugaçãoIntegridadeSêmenViabilidadeProtocols for cooling or freezing goat semen usually recommend centrifugation for seminal plasma removal. However, little is known about the effect of this process on goat sperm viability and functionality. The present study evaluated the effects of centrifugation force on the plasma membrane, acrosomes, and DNA integrity of goat semen. Four ejaculates from each of the four different Anglo Nubian male goats were used. Semen samples were obtained using artificial vagina, and immediately after collection, ejaculates were diluted using Ringer’s sodium lactate solution and split into three groups: Control (CG, without centrifugation), G1 (centrifugation 600 x g/10 min), G2 (centrifugation 1200 x g/10 min). After centrifugation, seminal plasma was removed, the sperm pellets were resuspended using Tris-egg yolk extender (80 x 106 spermatozoa/mL) and the sperm morphology was analyzed. Samples were cooled at 5°C for 5, 24, 36, and 48 h and then sperm plasma membrane and acrosome integrity (PMAI, %) and sperm DNA fragmentation index (SDF, %) were evaluated at each time-point, using a flow cytometer. Additionally, sperm movement was determined using computer semen analysis (CASA) after 5, 24, and 48 h of refrigeration period. The semen centrifugation did not induce additional sperm morphology defect or reduction in sperm kinetics in the experimental groups. Differences were not observed (p > 0.05) in PMAI and SDF among different groups, in any of each timepointof the cooling process. In conclusion, centrifugation, even at high speeds, did not affect goat sperm integrity and functionality when submitted to refrigeration process.A maior parte dos protocolos de refrigeração e criopreservação do sêmen caprino recomenda o uso de centrifugação para remoção do plasma seminal. No entanto, não existe consenso sobre o risco que esse tipo de processamento pode ocasionar à viabilidade espermática. Nesse contexto, o presente trabalho investigou os possíveis efeitos deletérios da centrifugação sobre a integridade estrutural e DNA de espermatozoides caprinos. Para a pesquisa foram selecionados quatro reprodutores para colheita de sêmen (n = 4 ejaculados/bode). Cada ejaculado foi fracionado em três alíquotas iguais, diluídas em ringer e divididas em três grupos: Controle (GC, não centrifugado), G1 (centrifugação a 600 g/10 minutos) e G2 (centrifugação a 1200 g/10 minutos). As amostras seminais por grupo foram diluídas em meio Tris gema respeitando-se a concentração final de 80 milhões de espermatozoides/mL e foram submetidas à avaliação de morfologia espermática. Todas as amostras foram acondicionadas a 5°C, sendo analisadas nos momentos 5, 24, 36 e 48 horas do processo de refrigeração por meio da avaliação da integridade de membrana plasmática e acrossomal (MPAI, %) e índice de fragmentação de DNA (IDF, %). Adicionalmente, a cinética espermática foi avaliada com o emprego de um sistema computadorizado de análise (CASA) nos momentos 5, 24 e 48 horas da refrigeração. A centrifugação não induziu a manifestação de defeitos morfológicos ou redução significativa da cinética de espermatozoides caprinos. Não foram observadas diferenças para a integridade de membrana plasmática e para o índice de fragmentação de DNA quando comparados, respectivamente, GC, G1 e G2 em cada um dos quatro momentos experimentais. Conclui-se que mesmo quando empregadas altas forças de rotação não ocorre lesão à ultraestrutura dos espermatozoides caprinos submetidos ao processo de refrigeração.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2018-12-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/14587310.11606/issn.1678-4456.bjvras.2018.145873Brazilian Journal of Veterinary Research and Animal Science; Vol. 55 Núm. 3 (2018); e145873Brazilian Journal of Veterinary Research and Animal Science; Vol. 55 No. 3 (2018); e145873Brazilian Journal of Veterinary Research and Animal Science; v. 55 n. 3 (2018); e145873Brazilian Journal of Veterinary Research and Animal Science; V. 55 N. 3 (2018); e1458731678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/145873/149058Copyright (c) 2018 Brazilian Journal of Veterinary Research and Animal Scienceinfo:eu-repo/semantics/openAccessCrespilho, André MacielBosco, Karinne ÁvilaDell'Aqua, Camila de Paula FreitasSegabinazzi, Lorenzo GarridoPapa, Frederico OzanamBrás, Karoline Maria GilGomes, Gustavo MendesPeixoto Junior, Kleber da Cunha2020-06-23T04:02:50Zoai:revistas.usp.br:article/145873Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:44:02.087634Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Can centrifugation force compromise the plasmatic membrane, acrosome and DNA integrity of goat spermatozoa? A força de centrifugação pode comprometer a integridade de membrana plasmática, acrossomal e DNA de espermatozoides caprinos? |
title |
Can centrifugation force compromise the plasmatic membrane, acrosome and DNA integrity of goat spermatozoa? |
spellingShingle |
Can centrifugation force compromise the plasmatic membrane, acrosome and DNA integrity of goat spermatozoa? Crespilho, André Maciel Centrifugation Goat Integrity Semen Viability Caprino Centrifugação Integridade Sêmen Viabilidade |
title_short |
Can centrifugation force compromise the plasmatic membrane, acrosome and DNA integrity of goat spermatozoa? |
title_full |
Can centrifugation force compromise the plasmatic membrane, acrosome and DNA integrity of goat spermatozoa? |
title_fullStr |
Can centrifugation force compromise the plasmatic membrane, acrosome and DNA integrity of goat spermatozoa? |
title_full_unstemmed |
Can centrifugation force compromise the plasmatic membrane, acrosome and DNA integrity of goat spermatozoa? |
title_sort |
Can centrifugation force compromise the plasmatic membrane, acrosome and DNA integrity of goat spermatozoa? |
author |
Crespilho, André Maciel |
author_facet |
Crespilho, André Maciel Bosco, Karinne Ávila Dell'Aqua, Camila de Paula Freitas Segabinazzi, Lorenzo Garrido Papa, Frederico Ozanam Brás, Karoline Maria Gil Gomes, Gustavo Mendes Peixoto Junior, Kleber da Cunha |
author_role |
author |
author2 |
Bosco, Karinne Ávila Dell'Aqua, Camila de Paula Freitas Segabinazzi, Lorenzo Garrido Papa, Frederico Ozanam Brás, Karoline Maria Gil Gomes, Gustavo Mendes Peixoto Junior, Kleber da Cunha |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Crespilho, André Maciel Bosco, Karinne Ávila Dell'Aqua, Camila de Paula Freitas Segabinazzi, Lorenzo Garrido Papa, Frederico Ozanam Brás, Karoline Maria Gil Gomes, Gustavo Mendes Peixoto Junior, Kleber da Cunha |
dc.subject.por.fl_str_mv |
Centrifugation Goat Integrity Semen Viability Caprino Centrifugação Integridade Sêmen Viabilidade |
topic |
Centrifugation Goat Integrity Semen Viability Caprino Centrifugação Integridade Sêmen Viabilidade |
description |
Protocols for cooling or freezing goat semen usually recommend centrifugation for seminal plasma removal. However, little is known about the effect of this process on goat sperm viability and functionality. The present study evaluated the effects of centrifugation force on the plasma membrane, acrosomes, and DNA integrity of goat semen. Four ejaculates from each of the four different Anglo Nubian male goats were used. Semen samples were obtained using artificial vagina, and immediately after collection, ejaculates were diluted using Ringer’s sodium lactate solution and split into three groups: Control (CG, without centrifugation), G1 (centrifugation 600 x g/10 min), G2 (centrifugation 1200 x g/10 min). After centrifugation, seminal plasma was removed, the sperm pellets were resuspended using Tris-egg yolk extender (80 x 106 spermatozoa/mL) and the sperm morphology was analyzed. Samples were cooled at 5°C for 5, 24, 36, and 48 h and then sperm plasma membrane and acrosome integrity (PMAI, %) and sperm DNA fragmentation index (SDF, %) were evaluated at each time-point, using a flow cytometer. Additionally, sperm movement was determined using computer semen analysis (CASA) after 5, 24, and 48 h of refrigeration period. The semen centrifugation did not induce additional sperm morphology defect or reduction in sperm kinetics in the experimental groups. Differences were not observed (p > 0.05) in PMAI and SDF among different groups, in any of each timepointof the cooling process. In conclusion, centrifugation, even at high speeds, did not affect goat sperm integrity and functionality when submitted to refrigeration process. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-12 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/145873 10.11606/issn.1678-4456.bjvras.2018.145873 |
url |
https://www.revistas.usp.br/bjvras/article/view/145873 |
identifier_str_mv |
10.11606/issn.1678-4456.bjvras.2018.145873 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/145873/149058 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Brazilian Journal of Veterinary Research and Animal Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Brazilian Journal of Veterinary Research and Animal Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 55 Núm. 3 (2018); e145873 Brazilian Journal of Veterinary Research and Animal Science; Vol. 55 No. 3 (2018); e145873 Brazilian Journal of Veterinary Research and Animal Science; v. 55 n. 3 (2018); e145873 Brazilian Journal of Veterinary Research and Animal Science; V. 55 N. 3 (2018); e145873 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051567333965824 |