Rep-PCR of Staphylococcus aureus strains isolated from bovine mastitis in Argentina

Detalhes bibliográficos
Autor(a) principal: Reinoso, Elina
Data de Publicação: 2007
Outros Autores: Bettera, Susana, Odierno, Liliana, Bogni, Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/26599
Resumo: The aim of this study was to assess by rep-PCR the genetic relationship of 52 S. aureus strains isolated from mammary infections collected in four herds located in the central dairy region of Argentina. Results were compared with the in vitro activity of antimicrobial drugs frequently used for treating bovine mastitis. Twelve different antimicrobials patterns were observed. Forty eight percent of the strains were susceptible to all antimicrobials tested. rep-PCR typing could successfully differentiate S. aureus strains of bovine origin. At a first level of similarity (50%), it could be defined 5 clusters namely I to V. Most of the strains (75%) were grouped in cluster I. The results may suggest that genotypes were similar in the different herds. Agreement between antibiotic patterns and rep-profiles was not observed for most isolates. The present report describes the genotypes responsible for the mastitis cases in the central dairy region of Argentina. A better knowledge of infective strains distribution in dairy herds might help in formulating strategies to control of infection. Furthermore, antimicrobial susceptibility of S. aureus should be used as guide to select effective drugs to therapy in intramammary infections.
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spelling Rep-PCR of Staphylococcus aureus strains isolated from bovine mastitis in ArgentinaRep-pcr de Staphylococcus aureus isolados a partir de mastite bovina na ArgentinaStaphylococcus aureusrep-PCRMastite bovinaArgentinaStaphylococcus aureusrep-PCRBovine mastitisArgentinaThe aim of this study was to assess by rep-PCR the genetic relationship of 52 S. aureus strains isolated from mammary infections collected in four herds located in the central dairy region of Argentina. Results were compared with the in vitro activity of antimicrobial drugs frequently used for treating bovine mastitis. Twelve different antimicrobials patterns were observed. Forty eight percent of the strains were susceptible to all antimicrobials tested. rep-PCR typing could successfully differentiate S. aureus strains of bovine origin. At a first level of similarity (50%), it could be defined 5 clusters namely I to V. Most of the strains (75%) were grouped in cluster I. The results may suggest that genotypes were similar in the different herds. Agreement between antibiotic patterns and rep-profiles was not observed for most isolates. The present report describes the genotypes responsible for the mastitis cases in the central dairy region of Argentina. A better knowledge of infective strains distribution in dairy herds might help in formulating strategies to control of infection. Furthermore, antimicrobial susceptibility of S. aureus should be used as guide to select effective drugs to therapy in intramammary infections.O objetivo do presente estudo foi determinar a relação genética entre rep-PCR de 52 linhagens Staphylococcus aureus isoladas de infecções mamárias em quatro fazendas leiteiras da região leiteira central da Argentina. Os resultados foram comparados com a atividade in vitro dos antimicrobianos freqüentemente utilizados no tratamento da mastite bovina. Foram observados 12 diferentes perfis de antimicrobianos. Do total de linhagens, 45% foram suscetíveis a todos os antibióticos ensaiados. A caracterizacão por rep-PCR pode diferenciar com sucesso as linhagens de S. aureus de origem bovina. Num primeiro nível de similaridade (50%) foram definidos cinco grupos denominados de I a V. A maioria das estirpes (75%) agruparam-se no grupo I. Os resultados sugerem que os genotipos foram similares. Os genotipos não foram asociados com os perfis de antimicrobianos na maioria dos isolados. O presente estudo descreve os genotipos responsáveis pelos casos de mastites na região central da Argentina. O melhor conhecimento da distribução das linhagens infectantes em fazendas leiteras poderia auxiliar na formulacão de estratégias para o controle de infecção. Além disso a suscetibilidade aos antimicrobianos de linhagens de S. aureus deve ser usada para montear a selecão de drogas efetivas para a terapia intramamária.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2007-12-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/2659910.11606/issn.1678-4456.bjvras.2007.26599Brazilian Journal of Veterinary Research and Animal Science; Vol. 44 (2007): Suplemento; 115-121Brazilian Journal of Veterinary Research and Animal Science; Vol. 44 (2007): Suplemento; 115-121Brazilian Journal of Veterinary Research and Animal Science; v. 44 (2007): Suplemento; 115-121Brazilian Journal of Veterinary Research and Animal Science; V. 44 (2007): Suplemento; 115-1211678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/26599/28382Reinoso, ElinaBettera, SusanaOdierno, LilianaBogni, Cristinainfo:eu-repo/semantics/openAccess2020-06-23T04:14:06Zoai:revistas.usp.br:article/26599Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:52.650947Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Rep-PCR of Staphylococcus aureus strains isolated from bovine mastitis in Argentina
Rep-pcr de Staphylococcus aureus isolados a partir de mastite bovina na Argentina
title Rep-PCR of Staphylococcus aureus strains isolated from bovine mastitis in Argentina
spellingShingle Rep-PCR of Staphylococcus aureus strains isolated from bovine mastitis in Argentina
Reinoso, Elina
Staphylococcus aureus
rep-PCR
Mastite bovina
Argentina
Staphylococcus aureus
rep-PCR
Bovine mastitis
Argentina
title_short Rep-PCR of Staphylococcus aureus strains isolated from bovine mastitis in Argentina
title_full Rep-PCR of Staphylococcus aureus strains isolated from bovine mastitis in Argentina
title_fullStr Rep-PCR of Staphylococcus aureus strains isolated from bovine mastitis in Argentina
title_full_unstemmed Rep-PCR of Staphylococcus aureus strains isolated from bovine mastitis in Argentina
title_sort Rep-PCR of Staphylococcus aureus strains isolated from bovine mastitis in Argentina
author Reinoso, Elina
author_facet Reinoso, Elina
Bettera, Susana
Odierno, Liliana
Bogni, Cristina
author_role author
author2 Bettera, Susana
Odierno, Liliana
Bogni, Cristina
author2_role author
author
author
dc.contributor.author.fl_str_mv Reinoso, Elina
Bettera, Susana
Odierno, Liliana
Bogni, Cristina
dc.subject.por.fl_str_mv Staphylococcus aureus
rep-PCR
Mastite bovina
Argentina
Staphylococcus aureus
rep-PCR
Bovine mastitis
Argentina
topic Staphylococcus aureus
rep-PCR
Mastite bovina
Argentina
Staphylococcus aureus
rep-PCR
Bovine mastitis
Argentina
description The aim of this study was to assess by rep-PCR the genetic relationship of 52 S. aureus strains isolated from mammary infections collected in four herds located in the central dairy region of Argentina. Results were compared with the in vitro activity of antimicrobial drugs frequently used for treating bovine mastitis. Twelve different antimicrobials patterns were observed. Forty eight percent of the strains were susceptible to all antimicrobials tested. rep-PCR typing could successfully differentiate S. aureus strains of bovine origin. At a first level of similarity (50%), it could be defined 5 clusters namely I to V. Most of the strains (75%) were grouped in cluster I. The results may suggest that genotypes were similar in the different herds. Agreement between antibiotic patterns and rep-profiles was not observed for most isolates. The present report describes the genotypes responsible for the mastitis cases in the central dairy region of Argentina. A better knowledge of infective strains distribution in dairy herds might help in formulating strategies to control of infection. Furthermore, antimicrobial susceptibility of S. aureus should be used as guide to select effective drugs to therapy in intramammary infections.
publishDate 2007
dc.date.none.fl_str_mv 2007-12-03
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/26599
10.11606/issn.1678-4456.bjvras.2007.26599
url https://www.revistas.usp.br/bjvras/article/view/26599
identifier_str_mv 10.11606/issn.1678-4456.bjvras.2007.26599
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/26599/28382
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 44 (2007): Suplemento; 115-121
Brazilian Journal of Veterinary Research and Animal Science; Vol. 44 (2007): Suplemento; 115-121
Brazilian Journal of Veterinary Research and Animal Science; v. 44 (2007): Suplemento; 115-121
Brazilian Journal of Veterinary Research and Animal Science; V. 44 (2007): Suplemento; 115-121
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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