Rep-PCR of Staphylococcus aureus strains isolated from bovine mastitis in Argentina
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/26599 |
Resumo: | The aim of this study was to assess by rep-PCR the genetic relationship of 52 S. aureus strains isolated from mammary infections collected in four herds located in the central dairy region of Argentina. Results were compared with the in vitro activity of antimicrobial drugs frequently used for treating bovine mastitis. Twelve different antimicrobials patterns were observed. Forty eight percent of the strains were susceptible to all antimicrobials tested. rep-PCR typing could successfully differentiate S. aureus strains of bovine origin. At a first level of similarity (50%), it could be defined 5 clusters namely I to V. Most of the strains (75%) were grouped in cluster I. The results may suggest that genotypes were similar in the different herds. Agreement between antibiotic patterns and rep-profiles was not observed for most isolates. The present report describes the genotypes responsible for the mastitis cases in the central dairy region of Argentina. A better knowledge of infective strains distribution in dairy herds might help in formulating strategies to control of infection. Furthermore, antimicrobial susceptibility of S. aureus should be used as guide to select effective drugs to therapy in intramammary infections. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Rep-PCR of Staphylococcus aureus strains isolated from bovine mastitis in ArgentinaRep-pcr de Staphylococcus aureus isolados a partir de mastite bovina na ArgentinaStaphylococcus aureusrep-PCRMastite bovinaArgentinaStaphylococcus aureusrep-PCRBovine mastitisArgentinaThe aim of this study was to assess by rep-PCR the genetic relationship of 52 S. aureus strains isolated from mammary infections collected in four herds located in the central dairy region of Argentina. Results were compared with the in vitro activity of antimicrobial drugs frequently used for treating bovine mastitis. Twelve different antimicrobials patterns were observed. Forty eight percent of the strains were susceptible to all antimicrobials tested. rep-PCR typing could successfully differentiate S. aureus strains of bovine origin. At a first level of similarity (50%), it could be defined 5 clusters namely I to V. Most of the strains (75%) were grouped in cluster I. The results may suggest that genotypes were similar in the different herds. Agreement between antibiotic patterns and rep-profiles was not observed for most isolates. The present report describes the genotypes responsible for the mastitis cases in the central dairy region of Argentina. A better knowledge of infective strains distribution in dairy herds might help in formulating strategies to control of infection. Furthermore, antimicrobial susceptibility of S. aureus should be used as guide to select effective drugs to therapy in intramammary infections.O objetivo do presente estudo foi determinar a relação genética entre rep-PCR de 52 linhagens Staphylococcus aureus isoladas de infecções mamárias em quatro fazendas leiteiras da região leiteira central da Argentina. Os resultados foram comparados com a atividade in vitro dos antimicrobianos freqüentemente utilizados no tratamento da mastite bovina. Foram observados 12 diferentes perfis de antimicrobianos. Do total de linhagens, 45% foram suscetíveis a todos os antibióticos ensaiados. A caracterizacão por rep-PCR pode diferenciar com sucesso as linhagens de S. aureus de origem bovina. Num primeiro nível de similaridade (50%) foram definidos cinco grupos denominados de I a V. A maioria das estirpes (75%) agruparam-se no grupo I. Os resultados sugerem que os genotipos foram similares. Os genotipos não foram asociados com os perfis de antimicrobianos na maioria dos isolados. O presente estudo descreve os genotipos responsáveis pelos casos de mastites na região central da Argentina. O melhor conhecimento da distribução das linhagens infectantes em fazendas leiteras poderia auxiliar na formulacão de estratégias para o controle de infecção. Além disso a suscetibilidade aos antimicrobianos de linhagens de S. aureus deve ser usada para montear a selecão de drogas efetivas para a terapia intramamária.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2007-12-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/2659910.11606/issn.1678-4456.bjvras.2007.26599Brazilian Journal of Veterinary Research and Animal Science; Vol. 44 (2007): Suplemento; 115-121Brazilian Journal of Veterinary Research and Animal Science; Vol. 44 (2007): Suplemento; 115-121Brazilian Journal of Veterinary Research and Animal Science; v. 44 (2007): Suplemento; 115-121Brazilian Journal of Veterinary Research and Animal Science; V. 44 (2007): Suplemento; 115-1211678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/26599/28382Reinoso, ElinaBettera, SusanaOdierno, LilianaBogni, Cristinainfo:eu-repo/semantics/openAccess2020-06-23T04:14:06Zoai:revistas.usp.br:article/26599Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:52.650947Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Rep-PCR of Staphylococcus aureus strains isolated from bovine mastitis in Argentina Rep-pcr de Staphylococcus aureus isolados a partir de mastite bovina na Argentina |
title |
Rep-PCR of Staphylococcus aureus strains isolated from bovine mastitis in Argentina |
spellingShingle |
Rep-PCR of Staphylococcus aureus strains isolated from bovine mastitis in Argentina Reinoso, Elina Staphylococcus aureus rep-PCR Mastite bovina Argentina Staphylococcus aureus rep-PCR Bovine mastitis Argentina |
title_short |
Rep-PCR of Staphylococcus aureus strains isolated from bovine mastitis in Argentina |
title_full |
Rep-PCR of Staphylococcus aureus strains isolated from bovine mastitis in Argentina |
title_fullStr |
Rep-PCR of Staphylococcus aureus strains isolated from bovine mastitis in Argentina |
title_full_unstemmed |
Rep-PCR of Staphylococcus aureus strains isolated from bovine mastitis in Argentina |
title_sort |
Rep-PCR of Staphylococcus aureus strains isolated from bovine mastitis in Argentina |
author |
Reinoso, Elina |
author_facet |
Reinoso, Elina Bettera, Susana Odierno, Liliana Bogni, Cristina |
author_role |
author |
author2 |
Bettera, Susana Odierno, Liliana Bogni, Cristina |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Reinoso, Elina Bettera, Susana Odierno, Liliana Bogni, Cristina |
dc.subject.por.fl_str_mv |
Staphylococcus aureus rep-PCR Mastite bovina Argentina Staphylococcus aureus rep-PCR Bovine mastitis Argentina |
topic |
Staphylococcus aureus rep-PCR Mastite bovina Argentina Staphylococcus aureus rep-PCR Bovine mastitis Argentina |
description |
The aim of this study was to assess by rep-PCR the genetic relationship of 52 S. aureus strains isolated from mammary infections collected in four herds located in the central dairy region of Argentina. Results were compared with the in vitro activity of antimicrobial drugs frequently used for treating bovine mastitis. Twelve different antimicrobials patterns were observed. Forty eight percent of the strains were susceptible to all antimicrobials tested. rep-PCR typing could successfully differentiate S. aureus strains of bovine origin. At a first level of similarity (50%), it could be defined 5 clusters namely I to V. Most of the strains (75%) were grouped in cluster I. The results may suggest that genotypes were similar in the different herds. Agreement between antibiotic patterns and rep-profiles was not observed for most isolates. The present report describes the genotypes responsible for the mastitis cases in the central dairy region of Argentina. A better knowledge of infective strains distribution in dairy herds might help in formulating strategies to control of infection. Furthermore, antimicrobial susceptibility of S. aureus should be used as guide to select effective drugs to therapy in intramammary infections. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-12-03 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26599 10.11606/issn.1678-4456.bjvras.2007.26599 |
url |
https://www.revistas.usp.br/bjvras/article/view/26599 |
identifier_str_mv |
10.11606/issn.1678-4456.bjvras.2007.26599 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26599/28382 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 44 (2007): Suplemento; 115-121 Brazilian Journal of Veterinary Research and Animal Science; Vol. 44 (2007): Suplemento; 115-121 Brazilian Journal of Veterinary Research and Animal Science; v. 44 (2007): Suplemento; 115-121 Brazilian Journal of Veterinary Research and Animal Science; V. 44 (2007): Suplemento; 115-121 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
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1797051558300483584 |