Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle
Autor(a) principal: | |
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Data de Publicação: | 1996 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/50180 |
Resumo: | Ten Holstein bovines, descendants from Ivanhoe line, have been analyzed. DNAs, purified from leukocytes of those animals, were submitted to Polymerase Chain Reaction technique (PCR) and digested by Hae III and Taq I restriction enzymes. Accordingly, the bovine leukocyte adhesion deficiency (BLAD) genomic diagnosis was established. The tests revealed that 2 animals were carriers and 8 were normal. Since the development of PCR methodology, a fast, practical and efficient way to select bulls in Artificial Insemination Centers has been through detection of carrier and normal animals. |
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oai:revistas.usp.br:article/50180 |
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USP-49 |
network_name_str |
Brazilian Journal of Veterinary Research and Animal Science |
repository_id_str |
https://www.revistas.usp.br/bjvras/index |
spelling |
Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattleUtilização de marcadores de DNA para o diagnóstico genômico de animais domésticos: 1. Detecção da mutação pontual causadora da Deficiência de Adesão de Leucócitos Bovinos (BLAD) em gado Holandês no BrasilLeucócitosBovinosDNA.LeukocytesBovineDNA.Ten Holstein bovines, descendants from Ivanhoe line, have been analyzed. DNAs, purified from leukocytes of those animals, were submitted to Polymerase Chain Reaction technique (PCR) and digested by Hae III and Taq I restriction enzymes. Accordingly, the bovine leukocyte adhesion deficiency (BLAD) genomic diagnosis was established. The tests revealed that 2 animals were carriers and 8 were normal. Since the development of PCR methodology, a fast, practical and efficient way to select bulls in Artificial Insemination Centers has been through detection of carrier and normal animals.Analisaram-se 10 bovinos da raça Holandesa, descendentes da linhagem Ivanhoe. Submeteram-se os DNAs, purificados a partir de leucócitos destes animais, à técnica de Polymerase Chain Reaction (PCR) e posterior digestão com as enzimas de restrição Hae III e Taq I. Estabeleceu-se, desta maneira, o diagnóstico genômico da Deficiência de Adesão de Leucócitos Bovinos (BLAD). Os exames revelaram que 2 animais eram portadores e 8, normais. A partir do desenvolvimento da metodologia de PCR, tornou-se disponível um método rápido, prático e eficiente para a seleção de touros em Centrais de Inseminação Artificial, por meio da detecção de animais portadores e normais.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia1996-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/5018010.11606/issn.2318-3659.v33i3p133-135Brazilian Journal of Veterinary Research and Animal Science; Vol. 33 Núm. 3 (1996); 133-135Brazilian Journal of Veterinary Research and Animal Science; Vol. 33 No. 3 (1996); 133-135Brazilian Journal of Veterinary Research and Animal Science; v. 33 n. 3 (1996); 133-135Brazilian Journal of Veterinary Research and Animal Science; V. 33 N. 3 (1996); 133-1351678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/50180/54295Garcia, José FernandoGurgel, André Sanches do AmaralVisintin, José AntonioLunge, Vagner RicardoHoetzel, Isidroinfo:eu-repo/semantics/openAccess2020-06-23T04:49:55Zoai:revistas.usp.br:article/50180Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:14.863592Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle Utilização de marcadores de DNA para o diagnóstico genômico de animais domésticos: 1. Detecção da mutação pontual causadora da Deficiência de Adesão de Leucócitos Bovinos (BLAD) em gado Holandês no Brasil |
title |
Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle |
spellingShingle |
Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle Garcia, José Fernando Leucócitos Bovinos DNA. Leukocytes Bovine DNA. |
title_short |
Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle |
title_full |
Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle |
title_fullStr |
Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle |
title_full_unstemmed |
Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle |
title_sort |
Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle |
author |
Garcia, José Fernando |
author_facet |
Garcia, José Fernando Gurgel, André Sanches do Amaral Visintin, José Antonio Lunge, Vagner Ricardo Hoetzel, Isidro |
author_role |
author |
author2 |
Gurgel, André Sanches do Amaral Visintin, José Antonio Lunge, Vagner Ricardo Hoetzel, Isidro |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Garcia, José Fernando Gurgel, André Sanches do Amaral Visintin, José Antonio Lunge, Vagner Ricardo Hoetzel, Isidro |
dc.subject.por.fl_str_mv |
Leucócitos Bovinos DNA. Leukocytes Bovine DNA. |
topic |
Leucócitos Bovinos DNA. Leukocytes Bovine DNA. |
description |
Ten Holstein bovines, descendants from Ivanhoe line, have been analyzed. DNAs, purified from leukocytes of those animals, were submitted to Polymerase Chain Reaction technique (PCR) and digested by Hae III and Taq I restriction enzymes. Accordingly, the bovine leukocyte adhesion deficiency (BLAD) genomic diagnosis was established. The tests revealed that 2 animals were carriers and 8 were normal. Since the development of PCR methodology, a fast, practical and efficient way to select bulls in Artificial Insemination Centers has been through detection of carrier and normal animals. |
publishDate |
1996 |
dc.date.none.fl_str_mv |
1996-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/50180 10.11606/issn.2318-3659.v33i3p133-135 |
url |
https://www.revistas.usp.br/bjvras/article/view/50180 |
identifier_str_mv |
10.11606/issn.2318-3659.v33i3p133-135 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/50180/54295 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 33 Núm. 3 (1996); 133-135 Brazilian Journal of Veterinary Research and Animal Science; Vol. 33 No. 3 (1996); 133-135 Brazilian Journal of Veterinary Research and Animal Science; v. 33 n. 3 (1996); 133-135 Brazilian Journal of Veterinary Research and Animal Science; V. 33 N. 3 (1996); 133-135 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051562568187904 |