Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle

Detalhes bibliográficos
Autor(a) principal: Garcia, José Fernando
Data de Publicação: 1996
Outros Autores: Gurgel, André Sanches do Amaral, Visintin, José Antonio, Lunge, Vagner Ricardo, Hoetzel, Isidro
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/50180
Resumo: Ten Holstein bovines, descendants from Ivanhoe line, have been analyzed. DNAs, purified from leukocytes of those animals, were submitted to Polymerase Chain Reaction technique (PCR) and digested by Hae III and Taq I restriction enzymes. Accordingly, the bovine leukocyte adhesion deficiency (BLAD) genomic diagnosis was established. The tests revealed that 2 animals were carriers and 8 were normal. Since the development of PCR methodology, a fast, practical and efficient way to select bulls in Artificial Insemination Centers has been through detection of carrier and normal animals.
id USP-49_9908efcb1823200cf8d5600723216a13
oai_identifier_str oai:revistas.usp.br:article/50180
network_acronym_str USP-49
network_name_str Brazilian Journal of Veterinary Research and Animal Science
repository_id_str https://www.revistas.usp.br/bjvras/index
spelling Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattleUtilização de marcadores de DNA para o diagnóstico genômico de animais domésticos: 1. Detecção da mutação pontual causadora da Deficiência de Adesão de Leucócitos Bovinos (BLAD) em gado Holandês no BrasilLeucócitosBovinosDNA.LeukocytesBovineDNA.Ten Holstein bovines, descendants from Ivanhoe line, have been analyzed. DNAs, purified from leukocytes of those animals, were submitted to Polymerase Chain Reaction technique (PCR) and digested by Hae III and Taq I restriction enzymes. Accordingly, the bovine leukocyte adhesion deficiency (BLAD) genomic diagnosis was established. The tests revealed that 2 animals were carriers and 8 were normal. Since the development of PCR methodology, a fast, practical and efficient way to select bulls in Artificial Insemination Centers has been through detection of carrier and normal animals.Analisaram-se 10 bovinos da raça Holandesa, descendentes da linhagem Ivanhoe. Submeteram-se os DNAs, purificados a partir de leucócitos destes animais, à técnica de Polymerase Chain Reaction (PCR) e posterior digestão com as enzimas de restrição Hae III e Taq I. Estabeleceu-se, desta maneira, o diagnóstico genômico da Deficiência de Adesão de Leucócitos Bovinos (BLAD). Os exames revelaram que 2 animais eram portadores e 8, normais. A partir do desenvolvimento da metodologia de PCR, tornou-se disponível um método rápido, prático e eficiente para a seleção de touros em Centrais de Inseminação Artificial, por meio da detecção de animais portadores e normais.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia1996-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/5018010.11606/issn.2318-3659.v33i3p133-135Brazilian Journal of Veterinary Research and Animal Science; Vol. 33 Núm. 3 (1996); 133-135Brazilian Journal of Veterinary Research and Animal Science; Vol. 33 No. 3 (1996); 133-135Brazilian Journal of Veterinary Research and Animal Science; v. 33 n. 3 (1996); 133-135Brazilian Journal of Veterinary Research and Animal Science; V. 33 N. 3 (1996); 133-1351678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/50180/54295Garcia, José FernandoGurgel, André Sanches do AmaralVisintin, José AntonioLunge, Vagner RicardoHoetzel, Isidroinfo:eu-repo/semantics/openAccess2020-06-23T04:49:55Zoai:revistas.usp.br:article/50180Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:14.863592Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle
Utilização de marcadores de DNA para o diagnóstico genômico de animais domésticos: 1. Detecção da mutação pontual causadora da Deficiência de Adesão de Leucócitos Bovinos (BLAD) em gado Holandês no Brasil
title Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle
spellingShingle Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle
Garcia, José Fernando
Leucócitos
Bovinos
DNA.
Leukocytes
Bovine
DNA.
title_short Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle
title_full Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle
title_fullStr Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle
title_full_unstemmed Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle
title_sort Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle
author Garcia, José Fernando
author_facet Garcia, José Fernando
Gurgel, André Sanches do Amaral
Visintin, José Antonio
Lunge, Vagner Ricardo
Hoetzel, Isidro
author_role author
author2 Gurgel, André Sanches do Amaral
Visintin, José Antonio
Lunge, Vagner Ricardo
Hoetzel, Isidro
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Garcia, José Fernando
Gurgel, André Sanches do Amaral
Visintin, José Antonio
Lunge, Vagner Ricardo
Hoetzel, Isidro
dc.subject.por.fl_str_mv Leucócitos
Bovinos
DNA.
Leukocytes
Bovine
DNA.
topic Leucócitos
Bovinos
DNA.
Leukocytes
Bovine
DNA.
description Ten Holstein bovines, descendants from Ivanhoe line, have been analyzed. DNAs, purified from leukocytes of those animals, were submitted to Polymerase Chain Reaction technique (PCR) and digested by Hae III and Taq I restriction enzymes. Accordingly, the bovine leukocyte adhesion deficiency (BLAD) genomic diagnosis was established. The tests revealed that 2 animals were carriers and 8 were normal. Since the development of PCR methodology, a fast, practical and efficient way to select bulls in Artificial Insemination Centers has been through detection of carrier and normal animals.
publishDate 1996
dc.date.none.fl_str_mv 1996-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/50180
10.11606/issn.2318-3659.v33i3p133-135
url https://www.revistas.usp.br/bjvras/article/view/50180
identifier_str_mv 10.11606/issn.2318-3659.v33i3p133-135
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/50180/54295
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 33 Núm. 3 (1996); 133-135
Brazilian Journal of Veterinary Research and Animal Science; Vol. 33 No. 3 (1996); 133-135
Brazilian Journal of Veterinary Research and Animal Science; v. 33 n. 3 (1996); 133-135
Brazilian Journal of Veterinary Research and Animal Science; V. 33 N. 3 (1996); 133-135
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
_version_ 1797051562568187904