Susceptibility and resistance to antimicrobial Staphylococcus aureus under biofilm
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/74034 |
Resumo: | Staphylococcus is an important agent of mastitis, especially when biofilm producers are related to a number of mechanisms of resistance to different types of infections. The objective of this research was to evaluate the susceptibility and resistance of Staphylococcus aureus strains isolates from cases of bovine mastitis and milking environment under conditions of biofilm, compared to three different concentrations in different antimicrobials. Thirty-two strains used were S. aureus that, due to the inhibition tests, minimal biofilm eradication concentration were tested using cephalexin, amoxicillin and rifampin at concentrations of 30 mg/mL, and 50 mg/mL 100 mg/mL for 12 hours, and efficiency of tests evaluated by counting the colony forming units and the microplate test. The results revealed that among the tested antibiotics cephalexin showed the best efficiency at the three concentrations tested, and rifampin and amoxicillin were more efficient at concentrations of 50 and 100 mg/mL. These data demonstrate the importance of targeted antibiotic therapy associated with the correct cleaning of milking equipment, thus, preventing the formation of biofilm, avoiding persistent microbial adhesion in equipment, and the spread of microorganisms by ascending channel teats among herd animals. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Susceptibility and resistance to antimicrobial Staphylococcus aureus under biofilmSusceptibilidade e resistência a antimicrobianos de Staphylococcus aureus em condições de biofilmeMastiteMicrorganismosTerapia antimicrobianaResistência microbianaMastitisMicroorganismsAntimicrobial therapyMicrobial resistanceStaphylococcus is an important agent of mastitis, especially when biofilm producers are related to a number of mechanisms of resistance to different types of infections. The objective of this research was to evaluate the susceptibility and resistance of Staphylococcus aureus strains isolates from cases of bovine mastitis and milking environment under conditions of biofilm, compared to three different concentrations in different antimicrobials. Thirty-two strains used were S. aureus that, due to the inhibition tests, minimal biofilm eradication concentration were tested using cephalexin, amoxicillin and rifampin at concentrations of 30 mg/mL, and 50 mg/mL 100 mg/mL for 12 hours, and efficiency of tests evaluated by counting the colony forming units and the microplate test. The results revealed that among the tested antibiotics cephalexin showed the best efficiency at the three concentrations tested, and rifampin and amoxicillin were more efficient at concentrations of 50 and 100 mg/mL. These data demonstrate the importance of targeted antibiotic therapy associated with the correct cleaning of milking equipment, thus, preventing the formation of biofilm, avoiding persistent microbial adhesion in equipment, and the spread of microorganisms by ascending channel teats among herd animals.Os Staphylococcus, principais agentes causadores da mastite, quando em biofilmes, estão relacionados a uma série de mecanismos de resistência em diferentes tipos de infecções. O presente trabalho avaliou a susceptibilidade e resistência dos Staphylococcus aureus, isolados de casos de mastite bovina e do ambiente de ordenha, em condições de biofilme, frente a três antimicrobianos distintos em diferentes concentrações. Foram utilizadas trinta e duas estirpes de S. aureus testadas frente aos testes de inibição da concentração mínima de erradicação de biofilmes, utilizando cefalexina, amoxicilina e rifampicina, nas concentrações de 30 mg/mL, 50 mg/mL e 100 mg/mL, por doze horas de contato, e a eficiência dos testes avaliada pela contagem das unidades formadoras de colônias e pelo teste de microplacas. Os resultados revelaram que, entre os antimicrobianos testados, a cefalexina foi o que apresentou melhor eficiência nas três concentrações testadas, e a rifampicina e amoxacilina tiveram maior eficiência nas concentrações de 50 e 100 mg/ mL. Esses dados demonstram a importância sobre antibioticoterapia orientada associada com a correta higienização dos equipamentos de ordenha, evitando-se, assim, a formação de biofilmes, a adesão microbiana persistente em equipamentos, e a difusão dos microrganismos pelo canal ascendente dos tetos entre os animais do rebanho.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2015-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/7403410.11606/issn.1678-4456.v52i3p228-233Brazilian Journal of Veterinary Research and Animal Science; Vol. 52 Núm. 3 (2015); 228-233Brazilian Journal of Veterinary Research and Animal Science; Vol. 52 No. 3 (2015); 228-233Brazilian Journal of Veterinary Research and Animal Science; v. 52 n. 3 (2015); 228-233Brazilian Journal of Veterinary Research and Animal Science; V. 52 N. 3 (2015); 228-2331678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/74034/103862Copyright (c) 2015 Brazilian Journal of Veterinary Research and Animal Scienceinfo:eu-repo/semantics/openAccessChagas, Laura Gonçalves da SilvaMelo, Poliana de CastroLima, Anna Monteiro CorreiaRamos, Gabriela BimRöder, Denise von Dolinger de BritoNader-Filho, Antônio2020-06-23T04:05:26Zoai:revistas.usp.br:article/74034Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:50.696394Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Susceptibility and resistance to antimicrobial Staphylococcus aureus under biofilm Susceptibilidade e resistência a antimicrobianos de Staphylococcus aureus em condições de biofilme |
title |
Susceptibility and resistance to antimicrobial Staphylococcus aureus under biofilm |
spellingShingle |
Susceptibility and resistance to antimicrobial Staphylococcus aureus under biofilm Chagas, Laura Gonçalves da Silva Mastite Microrganismos Terapia antimicrobiana Resistência microbiana Mastitis Microorganisms Antimicrobial therapy Microbial resistance |
title_short |
Susceptibility and resistance to antimicrobial Staphylococcus aureus under biofilm |
title_full |
Susceptibility and resistance to antimicrobial Staphylococcus aureus under biofilm |
title_fullStr |
Susceptibility and resistance to antimicrobial Staphylococcus aureus under biofilm |
title_full_unstemmed |
Susceptibility and resistance to antimicrobial Staphylococcus aureus under biofilm |
title_sort |
Susceptibility and resistance to antimicrobial Staphylococcus aureus under biofilm |
author |
Chagas, Laura Gonçalves da Silva |
author_facet |
Chagas, Laura Gonçalves da Silva Melo, Poliana de Castro Lima, Anna Monteiro Correia Ramos, Gabriela Bim Röder, Denise von Dolinger de Brito Nader-Filho, Antônio |
author_role |
author |
author2 |
Melo, Poliana de Castro Lima, Anna Monteiro Correia Ramos, Gabriela Bim Röder, Denise von Dolinger de Brito Nader-Filho, Antônio |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Chagas, Laura Gonçalves da Silva Melo, Poliana de Castro Lima, Anna Monteiro Correia Ramos, Gabriela Bim Röder, Denise von Dolinger de Brito Nader-Filho, Antônio |
dc.subject.por.fl_str_mv |
Mastite Microrganismos Terapia antimicrobiana Resistência microbiana Mastitis Microorganisms Antimicrobial therapy Microbial resistance |
topic |
Mastite Microrganismos Terapia antimicrobiana Resistência microbiana Mastitis Microorganisms Antimicrobial therapy Microbial resistance |
description |
Staphylococcus is an important agent of mastitis, especially when biofilm producers are related to a number of mechanisms of resistance to different types of infections. The objective of this research was to evaluate the susceptibility and resistance of Staphylococcus aureus strains isolates from cases of bovine mastitis and milking environment under conditions of biofilm, compared to three different concentrations in different antimicrobials. Thirty-two strains used were S. aureus that, due to the inhibition tests, minimal biofilm eradication concentration were tested using cephalexin, amoxicillin and rifampin at concentrations of 30 mg/mL, and 50 mg/mL 100 mg/mL for 12 hours, and efficiency of tests evaluated by counting the colony forming units and the microplate test. The results revealed that among the tested antibiotics cephalexin showed the best efficiency at the three concentrations tested, and rifampin and amoxicillin were more efficient at concentrations of 50 and 100 mg/mL. These data demonstrate the importance of targeted antibiotic therapy associated with the correct cleaning of milking equipment, thus, preventing the formation of biofilm, avoiding persistent microbial adhesion in equipment, and the spread of microorganisms by ascending channel teats among herd animals. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/74034 10.11606/issn.1678-4456.v52i3p228-233 |
url |
https://www.revistas.usp.br/bjvras/article/view/74034 |
identifier_str_mv |
10.11606/issn.1678-4456.v52i3p228-233 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/74034/103862 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2015 Brazilian Journal of Veterinary Research and Animal Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2015 Brazilian Journal of Veterinary Research and Animal Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 52 Núm. 3 (2015); 228-233 Brazilian Journal of Veterinary Research and Animal Science; Vol. 52 No. 3 (2015); 228-233 Brazilian Journal of Veterinary Research and Animal Science; v. 52 n. 3 (2015); 228-233 Brazilian Journal of Veterinary Research and Animal Science; V. 52 N. 3 (2015); 228-233 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
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1797051566457356288 |