Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new facts
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/59426 |
Resumo: | Polycyclic aromatic hydrocarbons are known carcinogens used in rodent experimental models. In this study, the carcinogen DMBA (7,12-dimethylbenzanthracene) was administered by gavage, diluted in corn oil, to female BALB / c mice at hebdomadary doses of 1 mg per animal for 1, 3, 6 or 9 weeks. Animals were weighed and monitored weekly until death. Remaining animals were euthanized at the age of 53 weeks. At necropsy, representative fragments of neoplasms were collected and routinely processed for histopathological analysis. Of all mice that received DMBA, 68.57% developed some type of tumor. Of the 70 mice treated with various doses of DMBA, 22 (31.43%) developed mammary tumors. The adenoacanthoma was the most commonly (18.75%) diagnosed histological type of breast cancer. Lung (15.71%), lymphoid tissue (11.43%), stomach (7.14%) and skin (2.86%) were also primary sites of tumor development. One third (33.33%) of the mice receiving 1 mg of DMBA developed lung cancer. Therefore, the administration of DMBA was shown to be an efficient model of carcinogenesis in mice, especially for the study of breast cancer, when using the highest dose, and lung, when using the lowest dose. Carcinogenesis models have been used for several purposes in cancer research. These results represent new facts for a classic carcinogenesis model. |
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Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new factsCarcinogenese quimica por DMBA (7,12-dimethylbenzanthracene) em camundongos femeas BALB/c: novos fatosCarcinogenesisDMBAMiceLung neoplasmsBreast neoplasmsCarcinogêneseDMBACamundongosNeoplasias pulmonaresNeoplasias de mamaPolycyclic aromatic hydrocarbons are known carcinogens used in rodent experimental models. In this study, the carcinogen DMBA (7,12-dimethylbenzanthracene) was administered by gavage, diluted in corn oil, to female BALB / c mice at hebdomadary doses of 1 mg per animal for 1, 3, 6 or 9 weeks. Animals were weighed and monitored weekly until death. Remaining animals were euthanized at the age of 53 weeks. At necropsy, representative fragments of neoplasms were collected and routinely processed for histopathological analysis. Of all mice that received DMBA, 68.57% developed some type of tumor. Of the 70 mice treated with various doses of DMBA, 22 (31.43%) developed mammary tumors. The adenoacanthoma was the most commonly (18.75%) diagnosed histological type of breast cancer. Lung (15.71%), lymphoid tissue (11.43%), stomach (7.14%) and skin (2.86%) were also primary sites of tumor development. One third (33.33%) of the mice receiving 1 mg of DMBA developed lung cancer. Therefore, the administration of DMBA was shown to be an efficient model of carcinogenesis in mice, especially for the study of breast cancer, when using the highest dose, and lung, when using the lowest dose. Carcinogenesis models have been used for several purposes in cancer research. These results represent new facts for a classic carcinogenesis model.Hidrocarbonetos policíclicos e aromáticos são carcinógenos usados em modelos experimentais em roedores. Neste estudo, o carcinógeno DMBA (7,12-dimetilbenzantraceno) foi administrado por gavagem, diluído em óleo de milho, para camundongos BALB/c em doses hebdomadárias de 1 mg por animal por 1, 3, 6 ou 9 semanas. Os animais foram pesados e monitorados semanalmente até a morte. Os animais remanescentes foram eutanasiados com a idade de 53 semanas. Na necroscopia, fragmentos representativos das neoplasias foram colhidos e rotineiramente processados para exame histopatológico. De todos os animais que receberam DMBA, 68,57% desenvolveram algum tipo de tumor. Entre os 70 camundongos tratados com diferentes doses de DMBA, 22 (31,43%) desenvolveram neoplasias mamárias. O adenoacantoma foi o tumor mamário mais comumente diagnosticado (18,75%). Pulmões (15,71%), tecido linfoide (11,43%), estômago (7,14%) e pele (2,86%) foram também locais primários de desenvolvimento de neoplasias. Um terço (33,33%) dos camundongos que receberam 1 mg de DMBA desenvolveram neoplasias pulmonares. Portanto, a administração de DMBA foi considerada um modelo eficiente de carcinogênese em camundongos, especialmente para o estudo de neoplasias mamárias, quando a maior dose é utilizada, e de neoplasias pulmonares, quando utilizada a menor dose. Os modelos de carcinogênese química têm sido usados para diversos estudos na pesquisa em câncer, os resultados aqui apresentados mostram novos fatos para um modelo clássico de carcinogênese.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2015-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/5942610.11606/issn.1678-4456.v52i2p125-133Brazilian Journal of Veterinary Research and Animal Science; Vol. 52 Núm. 2 (2015); 125-133Brazilian Journal of Veterinary Research and Animal Science; Vol. 52 No. 2 (2015); 125-133Brazilian Journal of Veterinary Research and Animal Science; v. 52 n. 2 (2015); 125-133Brazilian Journal of Veterinary Research and Animal Science; V. 52 N. 2 (2015); 125-1331678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/59426/98293Copyright (c) 2015 Brazilian Journal of Veterinary Research and Animal Scienceinfo:eu-repo/semantics/openAccessOliveira, Krishna Duro deAvanzo, Gabriela UlianaTedardi, Marcello VannucciRangel, Marcelo Monte MórAvanzo, José LuisFukumasu, HeidgeRao, Kurapati Venkata KesavaSinhorini, Idércio LuizDagli, Maria Lúcia Zaidan2020-06-23T04:05:40Zoai:revistas.usp.br:article/59426Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:33.039463Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new facts Carcinogenese quimica por DMBA (7,12-dimethylbenzanthracene) em camundongos femeas BALB/c: novos fatos |
title |
Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new facts |
spellingShingle |
Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new facts Oliveira, Krishna Duro de Carcinogenesis DMBA Mice Lung neoplasms Breast neoplasms Carcinogênese DMBA Camundongos Neoplasias pulmonares Neoplasias de mama |
title_short |
Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new facts |
title_full |
Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new facts |
title_fullStr |
Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new facts |
title_full_unstemmed |
Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new facts |
title_sort |
Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new facts |
author |
Oliveira, Krishna Duro de |
author_facet |
Oliveira, Krishna Duro de Avanzo, Gabriela Uliana Tedardi, Marcello Vannucci Rangel, Marcelo Monte Mór Avanzo, José Luis Fukumasu, Heidge Rao, Kurapati Venkata Kesava Sinhorini, Idércio Luiz Dagli, Maria Lúcia Zaidan |
author_role |
author |
author2 |
Avanzo, Gabriela Uliana Tedardi, Marcello Vannucci Rangel, Marcelo Monte Mór Avanzo, José Luis Fukumasu, Heidge Rao, Kurapati Venkata Kesava Sinhorini, Idércio Luiz Dagli, Maria Lúcia Zaidan |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Krishna Duro de Avanzo, Gabriela Uliana Tedardi, Marcello Vannucci Rangel, Marcelo Monte Mór Avanzo, José Luis Fukumasu, Heidge Rao, Kurapati Venkata Kesava Sinhorini, Idércio Luiz Dagli, Maria Lúcia Zaidan |
dc.subject.por.fl_str_mv |
Carcinogenesis DMBA Mice Lung neoplasms Breast neoplasms Carcinogênese DMBA Camundongos Neoplasias pulmonares Neoplasias de mama |
topic |
Carcinogenesis DMBA Mice Lung neoplasms Breast neoplasms Carcinogênese DMBA Camundongos Neoplasias pulmonares Neoplasias de mama |
description |
Polycyclic aromatic hydrocarbons are known carcinogens used in rodent experimental models. In this study, the carcinogen DMBA (7,12-dimethylbenzanthracene) was administered by gavage, diluted in corn oil, to female BALB / c mice at hebdomadary doses of 1 mg per animal for 1, 3, 6 or 9 weeks. Animals were weighed and monitored weekly until death. Remaining animals were euthanized at the age of 53 weeks. At necropsy, representative fragments of neoplasms were collected and routinely processed for histopathological analysis. Of all mice that received DMBA, 68.57% developed some type of tumor. Of the 70 mice treated with various doses of DMBA, 22 (31.43%) developed mammary tumors. The adenoacanthoma was the most commonly (18.75%) diagnosed histological type of breast cancer. Lung (15.71%), lymphoid tissue (11.43%), stomach (7.14%) and skin (2.86%) were also primary sites of tumor development. One third (33.33%) of the mice receiving 1 mg of DMBA developed lung cancer. Therefore, the administration of DMBA was shown to be an efficient model of carcinogenesis in mice, especially for the study of breast cancer, when using the highest dose, and lung, when using the lowest dose. Carcinogenesis models have been used for several purposes in cancer research. These results represent new facts for a classic carcinogenesis model. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/59426 10.11606/issn.1678-4456.v52i2p125-133 |
url |
https://www.revistas.usp.br/bjvras/article/view/59426 |
identifier_str_mv |
10.11606/issn.1678-4456.v52i2p125-133 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/59426/98293 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2015 Brazilian Journal of Veterinary Research and Animal Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2015 Brazilian Journal of Veterinary Research and Animal Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 52 Núm. 2 (2015); 125-133 Brazilian Journal of Veterinary Research and Animal Science; Vol. 52 No. 2 (2015); 125-133 Brazilian Journal of Veterinary Research and Animal Science; v. 52 n. 2 (2015); 125-133 Brazilian Journal of Veterinary Research and Animal Science; V. 52 N. 2 (2015); 125-133 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051564444090368 |