Induction of experimental mammary carcinogenesis in rats with 7,12-dimethylbenz(a)anthracene

Detalhes bibliográficos
Autor(a) principal: Barros,Alfredo Carlos S. D.
Data de Publicação: 2004
Outros Autores: Muranaka,Elisa Naomi K., Mori,Lincon Jo, Pelizon,Christina Helena T., Iriya,Kyoshi, Giocondo,Giovana, Pinotti,José Aristodemo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Hospital das Clínicas
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87812004000500006
Resumo: PURPOSE: To test an experimental model of chemical mammary carcinogenesis induction in rats. METHODS: Twenty young virgin Sprague-Dawley female rats, aged 47 days, received 20 mg of 7,12-dimethylbenz(a)anthracene (DMBA) intragastrically by gavage. Afterwards, at 8 and 13 weeks, their mammary glands were examined. At the end of the experiment, the animals were sacrificed, and the mammary tumors were measured and weighed. Tumor fragments were analyzed using light microscopy. RESULTS: Eight weeks after DMBA injection, 16 rats presented at least 1 breast tumor (80%). After 13 weeks, all of them (100%) developed breast carcinomas that were confirmed by histopathological analysis. CONCLUSION: This experimental animal model of chemical mammary induced carcinogenesis is feasible and can be used in further experiments on the role of tumorigenic biomodulator substances.
id USP-57_3b4cda53d620bd2ae5e2c3daa08933e1
oai_identifier_str oai:scielo:S0041-87812004000500006
network_acronym_str USP-57
network_name_str Revista do Hospital das Clínicas
repository_id_str
spelling Induction of experimental mammary carcinogenesis in rats with 7,12-dimethylbenz(a)anthraceneBreast cancerAnimal modelsCarcinogensDMBA-induced tumorsRat mammary carcinogenesisPURPOSE: To test an experimental model of chemical mammary carcinogenesis induction in rats. METHODS: Twenty young virgin Sprague-Dawley female rats, aged 47 days, received 20 mg of 7,12-dimethylbenz(a)anthracene (DMBA) intragastrically by gavage. Afterwards, at 8 and 13 weeks, their mammary glands were examined. At the end of the experiment, the animals were sacrificed, and the mammary tumors were measured and weighed. Tumor fragments were analyzed using light microscopy. RESULTS: Eight weeks after DMBA injection, 16 rats presented at least 1 breast tumor (80%). After 13 weeks, all of them (100%) developed breast carcinomas that were confirmed by histopathological analysis. CONCLUSION: This experimental animal model of chemical mammary induced carcinogenesis is feasible and can be used in further experiments on the role of tumorigenic biomodulator substances.Faculdade de Medicina / Universidade de São Paulo - FM/USP2004-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87812004000500006Revista do Hospital das Clínicas v.59 n.5 2004reponame:Revista do Hospital das Clínicasinstname:Universidade de São Paulo (USP)instacron:USP10.1590/S0041-87812004000500006info:eu-repo/semantics/openAccessBarros,Alfredo Carlos S. D.Muranaka,Elisa Naomi K.Mori,Lincon JoPelizon,Christina Helena T.Iriya,KyoshiGiocondo,GiovanaPinotti,José Aristodemoeng2004-10-29T00:00:00Zoai:scielo:S0041-87812004000500006Revistahttp://www.scielo.br/rhcPUBhttps://old.scielo.br/oai/scielo-oai.php||revista.hc@hcnet.usp.br1678-99030041-8781opendoar:2004-10-29T00:00Revista do Hospital das Clínicas - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Induction of experimental mammary carcinogenesis in rats with 7,12-dimethylbenz(a)anthracene
title Induction of experimental mammary carcinogenesis in rats with 7,12-dimethylbenz(a)anthracene
spellingShingle Induction of experimental mammary carcinogenesis in rats with 7,12-dimethylbenz(a)anthracene
Barros,Alfredo Carlos S. D.
Breast cancer
Animal models
Carcinogens
DMBA-induced tumors
Rat mammary carcinogenesis
title_short Induction of experimental mammary carcinogenesis in rats with 7,12-dimethylbenz(a)anthracene
title_full Induction of experimental mammary carcinogenesis in rats with 7,12-dimethylbenz(a)anthracene
title_fullStr Induction of experimental mammary carcinogenesis in rats with 7,12-dimethylbenz(a)anthracene
title_full_unstemmed Induction of experimental mammary carcinogenesis in rats with 7,12-dimethylbenz(a)anthracene
title_sort Induction of experimental mammary carcinogenesis in rats with 7,12-dimethylbenz(a)anthracene
author Barros,Alfredo Carlos S. D.
author_facet Barros,Alfredo Carlos S. D.
Muranaka,Elisa Naomi K.
Mori,Lincon Jo
Pelizon,Christina Helena T.
Iriya,Kyoshi
Giocondo,Giovana
Pinotti,José Aristodemo
author_role author
author2 Muranaka,Elisa Naomi K.
Mori,Lincon Jo
Pelizon,Christina Helena T.
Iriya,Kyoshi
Giocondo,Giovana
Pinotti,José Aristodemo
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barros,Alfredo Carlos S. D.
Muranaka,Elisa Naomi K.
Mori,Lincon Jo
Pelizon,Christina Helena T.
Iriya,Kyoshi
Giocondo,Giovana
Pinotti,José Aristodemo
dc.subject.por.fl_str_mv Breast cancer
Animal models
Carcinogens
DMBA-induced tumors
Rat mammary carcinogenesis
topic Breast cancer
Animal models
Carcinogens
DMBA-induced tumors
Rat mammary carcinogenesis
description PURPOSE: To test an experimental model of chemical mammary carcinogenesis induction in rats. METHODS: Twenty young virgin Sprague-Dawley female rats, aged 47 days, received 20 mg of 7,12-dimethylbenz(a)anthracene (DMBA) intragastrically by gavage. Afterwards, at 8 and 13 weeks, their mammary glands were examined. At the end of the experiment, the animals were sacrificed, and the mammary tumors were measured and weighed. Tumor fragments were analyzed using light microscopy. RESULTS: Eight weeks after DMBA injection, 16 rats presented at least 1 breast tumor (80%). After 13 weeks, all of them (100%) developed breast carcinomas that were confirmed by histopathological analysis. CONCLUSION: This experimental animal model of chemical mammary induced carcinogenesis is feasible and can be used in further experiments on the role of tumorigenic biomodulator substances.
publishDate 2004
dc.date.none.fl_str_mv 2004-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87812004000500006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87812004000500006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0041-87812004000500006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Faculdade de Medicina / Universidade de São Paulo - FM/USP
publisher.none.fl_str_mv Faculdade de Medicina / Universidade de São Paulo - FM/USP
dc.source.none.fl_str_mv Revista do Hospital das Clínicas v.59 n.5 2004
reponame:Revista do Hospital das Clínicas
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Revista do Hospital das Clínicas
collection Revista do Hospital das Clínicas
repository.name.fl_str_mv Revista do Hospital das Clínicas - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||revista.hc@hcnet.usp.br
_version_ 1754820894828527616

Registros relacionados