Perfil oxidativo e funcional de sêmen bovino criopreservado em diferentes estações do ano
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/73515 |
Resumo: | In general, Taurus bulls under tropical conditions demonstrate reduced fertility due to heat and oxidative stress on testicular tissue. This high incidence of sperm damage is generally enhanced by the large amount of polyunsaturated fatty acids (PUFA) naturally present in bull sperm. Despite PUFA increase cellular sensitivity to lipid peroxidation, they are essential for membrane fluidity and also promote high cellular protection during cryopreservation process. Some reports related that animals and plants provided from cold weather present higher cellular concentration of PUFA, so the present study aims to compare the effect of the season on sperm quality of Taurus and Zebu bulls. Cryoprotected semen samples of 10 Nelore (Bos taurus indicus) and 10 Simmental (Bos taurus taurus) bulls were analyzed during winter and summer seasons. After freezing-thawing process, semen samples were submitted to sperm motility and vigor analysis, to tests of plasma membrane integrity (MPI–Eosin/Nigrosin), acrosomal integrity (MAI-POPE), DNA fragmentation degree (DNAf-Comet Assay) and high mitochondrial activity (ACM-DAB). Moreover, frozen-thawed semen samples were induced to lipid peroxidation for measurement of thiobarbituric acid reactive substances (TBARS) and malondialdehyde (MDA) concentration. Higher post-thawing sperm motility (26.50% winter; 13.30% summer) and ACM (22.34% winter; 13.30% summer) were observed during the winter in Nelore group. In Simmental group, no differences were observed for the studied variables. It was concluded that, despite the heat stress, no seasonal effect on sperm quality was observed in Taurus cattle, which may be related to higher concentration of PUFA in seminal composition. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Perfil oxidativo e funcional de sêmen bovino criopreservado em diferentes estações do anoOxidative and functional status of bovine semen cryopreserved in different seasonsBovineSeasonThermal stressOxidative stressPolyunsaturated fatty acidsBovinoEstação do anoEstresse térmicoEstresse oxidativoÁcidos graxos poli-insaturadosIn general, Taurus bulls under tropical conditions demonstrate reduced fertility due to heat and oxidative stress on testicular tissue. This high incidence of sperm damage is generally enhanced by the large amount of polyunsaturated fatty acids (PUFA) naturally present in bull sperm. Despite PUFA increase cellular sensitivity to lipid peroxidation, they are essential for membrane fluidity and also promote high cellular protection during cryopreservation process. Some reports related that animals and plants provided from cold weather present higher cellular concentration of PUFA, so the present study aims to compare the effect of the season on sperm quality of Taurus and Zebu bulls. Cryoprotected semen samples of 10 Nelore (Bos taurus indicus) and 10 Simmental (Bos taurus taurus) bulls were analyzed during winter and summer seasons. After freezing-thawing process, semen samples were submitted to sperm motility and vigor analysis, to tests of plasma membrane integrity (MPI–Eosin/Nigrosin), acrosomal integrity (MAI-POPE), DNA fragmentation degree (DNAf-Comet Assay) and high mitochondrial activity (ACM-DAB). Moreover, frozen-thawed semen samples were induced to lipid peroxidation for measurement of thiobarbituric acid reactive substances (TBARS) and malondialdehyde (MDA) concentration. Higher post-thawing sperm motility (26.50% winter; 13.30% summer) and ACM (22.34% winter; 13.30% summer) were observed during the winter in Nelore group. In Simmental group, no differences were observed for the studied variables. It was concluded that, despite the heat stress, no seasonal effect on sperm quality was observed in Taurus cattle, which may be related to higher concentration of PUFA in seminal composition.Bovinos taurinos, em clima tropical, mostram menores índices de fertilidade devido ao estresse térmico e oxidativo testicular. Essa alta incidência de alterações espermáticas é potencializada pela grande quantidade de ácidos graxos poli-insaturados (PUFA) presentes nos espermatozoides taurinos. Embora os PUFA aumentem a sensibilidade celular à peroxidação lipídica, são essenciais para a maleabilidade de membrana e promovem maior proteção celular durante a criopreservação. Devido aos relatos de que animais e vegetais de clima frio possuem maior concentração de PUFA nas células, o presente estudo teve como objetivo comparar o efeito da estação do ano sobre a qualidade espermática de taurinos e zebuínos. Foram avaliadas amostras criopreservadas de 10 touros da raça Nelore (Bos taurus indicus) e 10 da raça Simental (Bos taurus taurus), coletadas durante inverno e verão. Após a descongelação, foram realizados testes espermáticos de motilidade e vigor, testes de integridade de membrana plasmática (MPI–Eosina/Nigrosina), integridade acrossomal (MAI-POPE), grau de fragmentação de DNA (DNAf-Ensaio Cometa) e atividade citoquímica mitocondrial (ACM-DAB). Adicionalmente, avaliou-se a suscetibilidade das células espermáticas à peroxidação lipídica induzida, pela mensuração das substâncias reativas ao ácido tiobarbitúrico (TBARS) e malondialdeído (MDA). No grupo Nelore, foi observada maior motilidade espermática pós-descongelação (26,50% inverno; 13,30% verão) e maior ACM (22,34% inverno; 13,30% verão) durante o inverno. No grupo Simental, não houve diferença de época do ano nas variáveis. Concluiu-se que, apesar de sofrerem maior estresse térmico, nos animais taurinos não foi observado efeito da estação do ano sobre a qualidade espermática, o que pode estar relacionado a uma maior concentração de PUFA em sua composição seminal.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2015-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/7351510.11606/issn.1678-4456.v52i2p134-140Brazilian Journal of Veterinary Research and Animal Science; Vol. 52 Núm. 2 (2015); 134-140Brazilian Journal of Veterinary Research and Animal Science; Vol. 52 No. 2 (2015); 134-140Brazilian Journal of Veterinary Research and Animal Science; v. 52 n. 2 (2015); 134-140Brazilian Journal of Veterinary Research and Animal Science; V. 52 N. 2 (2015); 134-1401678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/73515/98294Copyright (c) 2015 Brazilian Journal of Veterinary Research and Animal Scienceinfo:eu-repo/semantics/openAccessRodrigues, Mariana de PaulaTarragó, Octávio Fabián BaoArruda, Rubens Paes deOliveira, Leticia ZoccolaroBertolla, Ricardo PimentaNichi, MarcilioBarnabe, Valquiria Hyppolito2020-06-23T04:05:37Zoai:revistas.usp.br:article/73515Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:50.598025Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Perfil oxidativo e funcional de sêmen bovino criopreservado em diferentes estações do ano Oxidative and functional status of bovine semen cryopreserved in different seasons |
title |
Perfil oxidativo e funcional de sêmen bovino criopreservado em diferentes estações do ano |
spellingShingle |
Perfil oxidativo e funcional de sêmen bovino criopreservado em diferentes estações do ano Rodrigues, Mariana de Paula Bovine Season Thermal stress Oxidative stress Polyunsaturated fatty acids Bovino Estação do ano Estresse térmico Estresse oxidativo Ácidos graxos poli-insaturados |
title_short |
Perfil oxidativo e funcional de sêmen bovino criopreservado em diferentes estações do ano |
title_full |
Perfil oxidativo e funcional de sêmen bovino criopreservado em diferentes estações do ano |
title_fullStr |
Perfil oxidativo e funcional de sêmen bovino criopreservado em diferentes estações do ano |
title_full_unstemmed |
Perfil oxidativo e funcional de sêmen bovino criopreservado em diferentes estações do ano |
title_sort |
Perfil oxidativo e funcional de sêmen bovino criopreservado em diferentes estações do ano |
author |
Rodrigues, Mariana de Paula |
author_facet |
Rodrigues, Mariana de Paula Tarragó, Octávio Fabián Bao Arruda, Rubens Paes de Oliveira, Leticia Zoccolaro Bertolla, Ricardo Pimenta Nichi, Marcilio Barnabe, Valquiria Hyppolito |
author_role |
author |
author2 |
Tarragó, Octávio Fabián Bao Arruda, Rubens Paes de Oliveira, Leticia Zoccolaro Bertolla, Ricardo Pimenta Nichi, Marcilio Barnabe, Valquiria Hyppolito |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues, Mariana de Paula Tarragó, Octávio Fabián Bao Arruda, Rubens Paes de Oliveira, Leticia Zoccolaro Bertolla, Ricardo Pimenta Nichi, Marcilio Barnabe, Valquiria Hyppolito |
dc.subject.por.fl_str_mv |
Bovine Season Thermal stress Oxidative stress Polyunsaturated fatty acids Bovino Estação do ano Estresse térmico Estresse oxidativo Ácidos graxos poli-insaturados |
topic |
Bovine Season Thermal stress Oxidative stress Polyunsaturated fatty acids Bovino Estação do ano Estresse térmico Estresse oxidativo Ácidos graxos poli-insaturados |
description |
In general, Taurus bulls under tropical conditions demonstrate reduced fertility due to heat and oxidative stress on testicular tissue. This high incidence of sperm damage is generally enhanced by the large amount of polyunsaturated fatty acids (PUFA) naturally present in bull sperm. Despite PUFA increase cellular sensitivity to lipid peroxidation, they are essential for membrane fluidity and also promote high cellular protection during cryopreservation process. Some reports related that animals and plants provided from cold weather present higher cellular concentration of PUFA, so the present study aims to compare the effect of the season on sperm quality of Taurus and Zebu bulls. Cryoprotected semen samples of 10 Nelore (Bos taurus indicus) and 10 Simmental (Bos taurus taurus) bulls were analyzed during winter and summer seasons. After freezing-thawing process, semen samples were submitted to sperm motility and vigor analysis, to tests of plasma membrane integrity (MPI–Eosin/Nigrosin), acrosomal integrity (MAI-POPE), DNA fragmentation degree (DNAf-Comet Assay) and high mitochondrial activity (ACM-DAB). Moreover, frozen-thawed semen samples were induced to lipid peroxidation for measurement of thiobarbituric acid reactive substances (TBARS) and malondialdehyde (MDA) concentration. Higher post-thawing sperm motility (26.50% winter; 13.30% summer) and ACM (22.34% winter; 13.30% summer) were observed during the winter in Nelore group. In Simmental group, no differences were observed for the studied variables. It was concluded that, despite the heat stress, no seasonal effect on sperm quality was observed in Taurus cattle, which may be related to higher concentration of PUFA in seminal composition. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/73515 10.11606/issn.1678-4456.v52i2p134-140 |
url |
https://www.revistas.usp.br/bjvras/article/view/73515 |
identifier_str_mv |
10.11606/issn.1678-4456.v52i2p134-140 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/73515/98294 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2015 Brazilian Journal of Veterinary Research and Animal Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2015 Brazilian Journal of Veterinary Research and Animal Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 52 Núm. 2 (2015); 134-140 Brazilian Journal of Veterinary Research and Animal Science; Vol. 52 No. 2 (2015); 134-140 Brazilian Journal of Veterinary Research and Animal Science; v. 52 n. 2 (2015); 134-140 Brazilian Journal of Veterinary Research and Animal Science; V. 52 N. 2 (2015); 134-140 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051566451064832 |