Prevalence of antinuclear autoantibodies in the serum of normal blood dornors

• Autor(a) principal: Fernandez,Solange Assuncion Villagra
• Data de Publicação: 2003
• Outros Autores: Lobo,Alice Zoghbi Coelho, Oliveira,Zilda Najjar Prado de, Fukumori,Ligia Maria Ichimura, Périgo,Alexandre Marques, Rivitti,Evandro A.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista do Hospital das Clínicas
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87812003000600005
• topic: Autoantibodies; Healthy population; Rheumatic diseases; Antinuclear antibodies; Autoimmunity

OBJECTIVE:To examine the presence of serum antinuclear autoantibodies in a healthy population. METHODS: Serum of 500 normal blood donors between 18 and 60 years of age were tested for the presence of autoantibodies. Antinuclear antibodies were detected by indirect immunofluorescence technique using HEp-2 epithelial cells as the substrate. The presence of dnaN was detected by indirect immunofluorescence technique using Critidia lucillae as the substrate. Anti-SSA (RO), anti-SSB (LA), anti-Sm, and anti-RNP were determined by double radial immunodiffusion. RESULTS: In the evaluation of the presence of serum antibodies, antinuclear antibodies were detected in 22.6% of the sera. The presence of other antibodies was not significant. The majority of the titers were 1:40. CONCLUSION: The presence of autoantibodies is not necessarily pathologic and has to be related to the age group, gender, and clinical condition of the patient.