Life cycle of Trypanosoma cruzi (y strain) in mice
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Hospital das Clínicas |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87811999000500002 |
Resumo: | Since 1958, we have studied experimental Chagas' disease (CD) by subcutaneous inoculation of 1,000 blood forms of Trypanosoma cruzi (Y strain) in Balb/C. mice. Evolution of parasitemia remained constant, beginning on the 5th and 6th day of the disease, increasing progressively, achieving a maximum on about the 30th day. After another month, only a few forms were present, and they disappeared from the circulation after the third month, as determined from direct examination of slides and the use of a Neubauer Counting Chamber. These events coincided with the appearance of amastigote nests in the tissues (especially the cardiac ones), starting the first week, and following the Gauss parasitemia curve, but they were not in parallel until the chronic stage. In 1997, we began to note the following changes: Parasites appeared in the circulation during the first week and disappeared starting on the 7th day, and there was a coincident absence of the amastigote nests in the tissues. A careful study verified that young forms in the evolutionary cycle of T. cruzi (epi + amastigotes) began to appear alongside the trypomastigotes in the circulation on the 5th and 7th post-inoculation day. At the same time, rounded, oval, and spindle shapes were seen circulating through the capillaries and sinusoids of the tissues, principally of the hematopoietic organs. Stasis occurs because the diameter of the circulating parasites is greater than the vessels, and this makes them more visible. Examination of the sternal bone marrow revealed young cells with elongated forms and others truncated in the shape of a "C" occupying the internal surface of the blood cells that had empty central portions (erythrocytes?). We hypothesize that there could be a loss of virulence or mutation of the Y strain of Trypanosoma cruzi. |
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Life cycle of Trypanosoma cruzi (y strain) in miceExperimental Chagas' diseaseAmerican trypanosomosisAmastigotesEpimastigotesTrypomastigotesSince 1958, we have studied experimental Chagas' disease (CD) by subcutaneous inoculation of 1,000 blood forms of Trypanosoma cruzi (Y strain) in Balb/C. mice. Evolution of parasitemia remained constant, beginning on the 5th and 6th day of the disease, increasing progressively, achieving a maximum on about the 30th day. After another month, only a few forms were present, and they disappeared from the circulation after the third month, as determined from direct examination of slides and the use of a Neubauer Counting Chamber. These events coincided with the appearance of amastigote nests in the tissues (especially the cardiac ones), starting the first week, and following the Gauss parasitemia curve, but they were not in parallel until the chronic stage. In 1997, we began to note the following changes: Parasites appeared in the circulation during the first week and disappeared starting on the 7th day, and there was a coincident absence of the amastigote nests in the tissues. A careful study verified that young forms in the evolutionary cycle of T. cruzi (epi + amastigotes) began to appear alongside the trypomastigotes in the circulation on the 5th and 7th post-inoculation day. At the same time, rounded, oval, and spindle shapes were seen circulating through the capillaries and sinusoids of the tissues, principally of the hematopoietic organs. Stasis occurs because the diameter of the circulating parasites is greater than the vessels, and this makes them more visible. Examination of the sternal bone marrow revealed young cells with elongated forms and others truncated in the shape of a "C" occupying the internal surface of the blood cells that had empty central portions (erythrocytes?). We hypothesize that there could be a loss of virulence or mutation of the Y strain of Trypanosoma cruzi.Faculdade de Medicina / Universidade de São Paulo - FM/USP1999-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87811999000500002Revista do Hospital das Clínicas v.54 n.5 1999reponame:Revista do Hospital das Clínicasinstname:Universidade de São Paulo (USP)instacron:USP10.1590/S0041-87811999000500002info:eu-repo/semantics/openAccessPinto,Pedro Luiz SilvaTakami,RobertoNunes,Elizabeth V.Guilherme,Carmem S.Oliveira Jr.,Oswaldo CruzGama-Rodrigues,JoaquimOkumura,Masayukieng2000-04-28T00:00:00Zoai:scielo:S0041-87811999000500002Revistahttp://www.scielo.br/rhcPUBhttps://old.scielo.br/oai/scielo-oai.php||revista.hc@hcnet.usp.br1678-99030041-8781opendoar:2000-04-28T00:00Revista do Hospital das Clínicas - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Life cycle of Trypanosoma cruzi (y strain) in mice |
title |
Life cycle of Trypanosoma cruzi (y strain) in mice |
spellingShingle |
Life cycle of Trypanosoma cruzi (y strain) in mice Pinto,Pedro Luiz Silva Experimental Chagas' disease American trypanosomosis Amastigotes Epimastigotes Trypomastigotes |
title_short |
Life cycle of Trypanosoma cruzi (y strain) in mice |
title_full |
Life cycle of Trypanosoma cruzi (y strain) in mice |
title_fullStr |
Life cycle of Trypanosoma cruzi (y strain) in mice |
title_full_unstemmed |
Life cycle of Trypanosoma cruzi (y strain) in mice |
title_sort |
Life cycle of Trypanosoma cruzi (y strain) in mice |
author |
Pinto,Pedro Luiz Silva |
author_facet |
Pinto,Pedro Luiz Silva Takami,Roberto Nunes,Elizabeth V. Guilherme,Carmem S. Oliveira Jr.,Oswaldo Cruz Gama-Rodrigues,Joaquim Okumura,Masayuki |
author_role |
author |
author2 |
Takami,Roberto Nunes,Elizabeth V. Guilherme,Carmem S. Oliveira Jr.,Oswaldo Cruz Gama-Rodrigues,Joaquim Okumura,Masayuki |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Pinto,Pedro Luiz Silva Takami,Roberto Nunes,Elizabeth V. Guilherme,Carmem S. Oliveira Jr.,Oswaldo Cruz Gama-Rodrigues,Joaquim Okumura,Masayuki |
dc.subject.por.fl_str_mv |
Experimental Chagas' disease American trypanosomosis Amastigotes Epimastigotes Trypomastigotes |
topic |
Experimental Chagas' disease American trypanosomosis Amastigotes Epimastigotes Trypomastigotes |
description |
Since 1958, we have studied experimental Chagas' disease (CD) by subcutaneous inoculation of 1,000 blood forms of Trypanosoma cruzi (Y strain) in Balb/C. mice. Evolution of parasitemia remained constant, beginning on the 5th and 6th day of the disease, increasing progressively, achieving a maximum on about the 30th day. After another month, only a few forms were present, and they disappeared from the circulation after the third month, as determined from direct examination of slides and the use of a Neubauer Counting Chamber. These events coincided with the appearance of amastigote nests in the tissues (especially the cardiac ones), starting the first week, and following the Gauss parasitemia curve, but they were not in parallel until the chronic stage. In 1997, we began to note the following changes: Parasites appeared in the circulation during the first week and disappeared starting on the 7th day, and there was a coincident absence of the amastigote nests in the tissues. A careful study verified that young forms in the evolutionary cycle of T. cruzi (epi + amastigotes) began to appear alongside the trypomastigotes in the circulation on the 5th and 7th post-inoculation day. At the same time, rounded, oval, and spindle shapes were seen circulating through the capillaries and sinusoids of the tissues, principally of the hematopoietic organs. Stasis occurs because the diameter of the circulating parasites is greater than the vessels, and this makes them more visible. Examination of the sternal bone marrow revealed young cells with elongated forms and others truncated in the shape of a "C" occupying the internal surface of the blood cells that had empty central portions (erythrocytes?). We hypothesize that there could be a loss of virulence or mutation of the Y strain of Trypanosoma cruzi. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87811999000500002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87811999000500002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0041-87811999000500002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Faculdade de Medicina / Universidade de São Paulo - FM/USP |
publisher.none.fl_str_mv |
Faculdade de Medicina / Universidade de São Paulo - FM/USP |
dc.source.none.fl_str_mv |
Revista do Hospital das Clínicas v.54 n.5 1999 reponame:Revista do Hospital das Clínicas instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Revista do Hospital das Clínicas |
collection |
Revista do Hospital das Clínicas |
repository.name.fl_str_mv |
Revista do Hospital das Clínicas - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||revista.hc@hcnet.usp.br |
_version_ |
1754820893992812544 |