Life cycle of Trypanosoma cruzi (y strain) in mice

Detalhes bibliográficos
Autor(a) principal: Pinto,Pedro Luiz Silva
Data de Publicação: 1999
Outros Autores: Takami,Roberto, Nunes,Elizabeth V., Guilherme,Carmem S., Oliveira Jr.,Oswaldo Cruz, Gama-Rodrigues,Joaquim, Okumura,Masayuki
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Hospital das Clínicas
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87811999000500002
Resumo: Since 1958, we have studied experimental Chagas' disease (CD) by subcutaneous inoculation of 1,000 blood forms of Trypanosoma cruzi (Y strain) in Balb/C. mice. Evolution of parasitemia remained constant, beginning on the 5th and 6th day of the disease, increasing progressively, achieving a maximum on about the 30th day. After another month, only a few forms were present, and they disappeared from the circulation after the third month, as determined from direct examination of slides and the use of a Neubauer Counting Chamber. These events coincided with the appearance of amastigote nests in the tissues (especially the cardiac ones), starting the first week, and following the Gauss parasitemia curve, but they were not in parallel until the chronic stage. In 1997, we began to note the following changes: Parasites appeared in the circulation during the first week and disappeared starting on the 7th day, and there was a coincident absence of the amastigote nests in the tissues. A careful study verified that young forms in the evolutionary cycle of T. cruzi (epi + amastigotes) began to appear alongside the trypomastigotes in the circulation on the 5th and 7th post-inoculation day. At the same time, rounded, oval, and spindle shapes were seen circulating through the capillaries and sinusoids of the tissues, principally of the hematopoietic organs. Stasis occurs because the diameter of the circulating parasites is greater than the vessels, and this makes them more visible. Examination of the sternal bone marrow revealed young cells with elongated forms and others truncated in the shape of a "C" occupying the internal surface of the blood cells that had empty central portions (erythrocytes?). We hypothesize that there could be a loss of virulence or mutation of the Y strain of Trypanosoma cruzi.
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spelling Life cycle of Trypanosoma cruzi (y strain) in miceExperimental Chagas' diseaseAmerican trypanosomosisAmastigotesEpimastigotesTrypomastigotesSince 1958, we have studied experimental Chagas' disease (CD) by subcutaneous inoculation of 1,000 blood forms of Trypanosoma cruzi (Y strain) in Balb/C. mice. Evolution of parasitemia remained constant, beginning on the 5th and 6th day of the disease, increasing progressively, achieving a maximum on about the 30th day. After another month, only a few forms were present, and they disappeared from the circulation after the third month, as determined from direct examination of slides and the use of a Neubauer Counting Chamber. These events coincided with the appearance of amastigote nests in the tissues (especially the cardiac ones), starting the first week, and following the Gauss parasitemia curve, but they were not in parallel until the chronic stage. In 1997, we began to note the following changes: Parasites appeared in the circulation during the first week and disappeared starting on the 7th day, and there was a coincident absence of the amastigote nests in the tissues. A careful study verified that young forms in the evolutionary cycle of T. cruzi (epi + amastigotes) began to appear alongside the trypomastigotes in the circulation on the 5th and 7th post-inoculation day. At the same time, rounded, oval, and spindle shapes were seen circulating through the capillaries and sinusoids of the tissues, principally of the hematopoietic organs. Stasis occurs because the diameter of the circulating parasites is greater than the vessels, and this makes them more visible. Examination of the sternal bone marrow revealed young cells with elongated forms and others truncated in the shape of a "C" occupying the internal surface of the blood cells that had empty central portions (erythrocytes?). We hypothesize that there could be a loss of virulence or mutation of the Y strain of Trypanosoma cruzi.Faculdade de Medicina / Universidade de São Paulo - FM/USP1999-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87811999000500002Revista do Hospital das Clínicas v.54 n.5 1999reponame:Revista do Hospital das Clínicasinstname:Universidade de São Paulo (USP)instacron:USP10.1590/S0041-87811999000500002info:eu-repo/semantics/openAccessPinto,Pedro Luiz SilvaTakami,RobertoNunes,Elizabeth V.Guilherme,Carmem S.Oliveira Jr.,Oswaldo CruzGama-Rodrigues,JoaquimOkumura,Masayukieng2000-04-28T00:00:00Zoai:scielo:S0041-87811999000500002Revistahttp://www.scielo.br/rhcPUBhttps://old.scielo.br/oai/scielo-oai.php||revista.hc@hcnet.usp.br1678-99030041-8781opendoar:2000-04-28T00:00Revista do Hospital das Clínicas - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Life cycle of Trypanosoma cruzi (y strain) in mice
title Life cycle of Trypanosoma cruzi (y strain) in mice
spellingShingle Life cycle of Trypanosoma cruzi (y strain) in mice
Pinto,Pedro Luiz Silva
Experimental Chagas' disease
American trypanosomosis
Amastigotes
Epimastigotes
Trypomastigotes
title_short Life cycle of Trypanosoma cruzi (y strain) in mice
title_full Life cycle of Trypanosoma cruzi (y strain) in mice
title_fullStr Life cycle of Trypanosoma cruzi (y strain) in mice
title_full_unstemmed Life cycle of Trypanosoma cruzi (y strain) in mice
title_sort Life cycle of Trypanosoma cruzi (y strain) in mice
author Pinto,Pedro Luiz Silva
author_facet Pinto,Pedro Luiz Silva
Takami,Roberto
Nunes,Elizabeth V.
Guilherme,Carmem S.
Oliveira Jr.,Oswaldo Cruz
Gama-Rodrigues,Joaquim
Okumura,Masayuki
author_role author
author2 Takami,Roberto
Nunes,Elizabeth V.
Guilherme,Carmem S.
Oliveira Jr.,Oswaldo Cruz
Gama-Rodrigues,Joaquim
Okumura,Masayuki
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pinto,Pedro Luiz Silva
Takami,Roberto
Nunes,Elizabeth V.
Guilherme,Carmem S.
Oliveira Jr.,Oswaldo Cruz
Gama-Rodrigues,Joaquim
Okumura,Masayuki
dc.subject.por.fl_str_mv Experimental Chagas' disease
American trypanosomosis
Amastigotes
Epimastigotes
Trypomastigotes
topic Experimental Chagas' disease
American trypanosomosis
Amastigotes
Epimastigotes
Trypomastigotes
description Since 1958, we have studied experimental Chagas' disease (CD) by subcutaneous inoculation of 1,000 blood forms of Trypanosoma cruzi (Y strain) in Balb/C. mice. Evolution of parasitemia remained constant, beginning on the 5th and 6th day of the disease, increasing progressively, achieving a maximum on about the 30th day. After another month, only a few forms were present, and they disappeared from the circulation after the third month, as determined from direct examination of slides and the use of a Neubauer Counting Chamber. These events coincided with the appearance of amastigote nests in the tissues (especially the cardiac ones), starting the first week, and following the Gauss parasitemia curve, but they were not in parallel until the chronic stage. In 1997, we began to note the following changes: Parasites appeared in the circulation during the first week and disappeared starting on the 7th day, and there was a coincident absence of the amastigote nests in the tissues. A careful study verified that young forms in the evolutionary cycle of T. cruzi (epi + amastigotes) began to appear alongside the trypomastigotes in the circulation on the 5th and 7th post-inoculation day. At the same time, rounded, oval, and spindle shapes were seen circulating through the capillaries and sinusoids of the tissues, principally of the hematopoietic organs. Stasis occurs because the diameter of the circulating parasites is greater than the vessels, and this makes them more visible. Examination of the sternal bone marrow revealed young cells with elongated forms and others truncated in the shape of a "C" occupying the internal surface of the blood cells that had empty central portions (erythrocytes?). We hypothesize that there could be a loss of virulence or mutation of the Y strain of Trypanosoma cruzi.
publishDate 1999
dc.date.none.fl_str_mv 1999-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87811999000500002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87811999000500002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0041-87811999000500002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Faculdade de Medicina / Universidade de São Paulo - FM/USP
publisher.none.fl_str_mv Faculdade de Medicina / Universidade de São Paulo - FM/USP
dc.source.none.fl_str_mv Revista do Hospital das Clínicas v.54 n.5 1999
reponame:Revista do Hospital das Clínicas
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Revista do Hospital das Clínicas
collection Revista do Hospital das Clínicas
repository.name.fl_str_mv Revista do Hospital das Clínicas - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||revista.hc@hcnet.usp.br
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