Comparative transcriptome profiling of virulent and non-virulent Trypanosoma cruzi underlines the role of surface proteins during infection
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/25171 |
Resumo: | University of Maryland. College Park. Department of Cell Biology and Molecular Genetics and Center for Bioinformatics and Computational Biology. Maryland, United States of America |
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Belew, Ashton TreyGiusta, Caroline JunqueiraLuiz, Gabriela Flavia RodriguesValente, Bruna MattiolyOliveira, Antonio Edson RochaPolidoro, Rafael BarbosaZuccherato, Luciana WerneckBartholomeu, Daniella CastanheiraSchenkman, SergioGazzinelli, Ricardo TostesBurleigh, Barbara AEl-Sayed, Najib M.Teixeira, Santuza Maria Ribeiro2018-03-07T17:07:07Z2018-03-07T17:07:07Z2017BELEW, Ashton Trey et al. Comparative transcriptome profiling of virulent and non-virulent Trypanosoma cruzi underlines the role of surface proteins during infection. PLoS Pathog., v. 13, n. 12, art. e1006767, 2017.1553-7366https://www.arca.fiocruz.br/handle/icict/2517110.1371/journal.ppat.1006767engPublic Library of ScienceAmastigotasTrypanosoma cruziAmastigotesTrypomastigotesGene expressionParasitic diseasesTrypanosoma cruziCell differentiationEpimastigotesComparative genomicComparative transcriptome profiling of virulent and non-virulent Trypanosoma cruzi underlines the role of surface proteins during infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of Maryland. College Park. Department of Cell Biology and Molecular Genetics and Center for Bioinformatics and Computational Biology. Maryland, United States of AmericaFundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilUniversidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrazilUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrazilFundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilHarvard T. H. Chan School of Public Health. Department of Immunology and Infectious Diseases. Boston, Massachusetts, United States of AmericaUniversity of Maryland. College Park. Department of Cell Biology and Molecular Genetics and Center for Bioinformatics and Computational Biology. Maryland, United States of AmericaUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilTrypanosoma cruzi, the protozoan that causes Chagas disease, has a complex life cycle involving several morphologically and biochemically distinct stages that establish intricate interactions with various insect and mammalian hosts. It has also a heterogeneous population structure comprising strains with distinct properties such as virulence, sensitivity to drugs, antigenic profile and tissue tropism. We present a comparative transcriptome analysis of two cloned T. cruzi strains that display contrasting virulence phenotypes in animal models of infection: CL Brener is a virulent clone and CL-14 is a clone that is neither infective nor pathogenic in in vivo models of infection. Gene expression analysis of trypomastigotes and intracellular amastigotes harvested at 60 and 96 hours post-infection (hpi) of human fibroblasts revealed large differences that reflect the parasite’s adaptation to distinct environments during the infection of mammalian cells, including changes in energy sources, oxidative stress responses, cell cycle control and cell surface components. While extensive transcriptome remodeling was observed when trypomastigotes of both strains were compared to 60 hpi amastigotes, differences in gene expression were much less pronounced when 96 hpi amastigotes and trypomastigotes of CL Brener were compared. In contrast, the differentiation of the avirulent CL-14 from 96 hpi amastigotes to extracellular trypomastigotes was associated with considerable changes in gene expression, particularly in gene families encoding surface proteins such as trans-sialidases, mucins and the mucin associated surface proteins (MASPs). Thus, our comparative transcriptome analysis indicates that the avirulent phenotype of CL-14 may be due, at least in part, to a reduced or delayed expression of genes encoding surface proteins that are associated with the transition of amastigotes to trypomastigotes, an essential step in the establishment of the infection in the mammalian host. Confirming the role of members of the trans-sialidase family of surface proteins for parasite differentiation, transfected CL-14 constitutively expressing a trans-sialidase gene displayed faster kinetics of trypomastigote release in the supernatant of infected cells compared to wild type CL-14info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/25171/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALComparative transcriptome profiling of.pdfComparative transcriptome profiling of.pdfapplication/pdf5603344https://www.arca.fiocruz.br/bitstream/icict/25171/2/Comparative%20transcriptome%20profiling%20of.pdf9b176b974de6026b6db15f0e824d5bbeMD52TEXTComparative transcriptome profiling of.pdf.txtComparative transcriptome profiling of.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Comparative transcriptome profiling of virulent and non-virulent Trypanosoma cruzi underlines the role of surface proteins during infection |
| title |
Comparative transcriptome profiling of virulent and non-virulent Trypanosoma cruzi underlines the role of surface proteins during infection |
| spellingShingle |
Comparative transcriptome profiling of virulent and non-virulent Trypanosoma cruzi underlines the role of surface proteins during infection Belew, Ashton Trey Amastigotas Trypanosoma cruzi Amastigotes Trypomastigotes Gene expression Parasitic diseases Trypanosoma cruzi Cell differentiation Epimastigotes Comparative genomic |
| title_short |
Comparative transcriptome profiling of virulent and non-virulent Trypanosoma cruzi underlines the role of surface proteins during infection |
| title_full |
Comparative transcriptome profiling of virulent and non-virulent Trypanosoma cruzi underlines the role of surface proteins during infection |
| title_fullStr |
Comparative transcriptome profiling of virulent and non-virulent Trypanosoma cruzi underlines the role of surface proteins during infection |
| title_full_unstemmed |
Comparative transcriptome profiling of virulent and non-virulent Trypanosoma cruzi underlines the role of surface proteins during infection |
| title_sort |
Comparative transcriptome profiling of virulent and non-virulent Trypanosoma cruzi underlines the role of surface proteins during infection |
| author |
Belew, Ashton Trey |
| author_facet |
Belew, Ashton Trey Giusta, Caroline Junqueira Luiz, Gabriela Flavia Rodrigues Valente, Bruna Mattioly Oliveira, Antonio Edson Rocha Polidoro, Rafael Barbosa Zuccherato, Luciana Werneck Bartholomeu, Daniella Castanheira Schenkman, Sergio Gazzinelli, Ricardo Tostes Burleigh, Barbara A El-Sayed, Najib M. Teixeira, Santuza Maria Ribeiro |
| author_role |
author |
| author2 |
Giusta, Caroline Junqueira Luiz, Gabriela Flavia Rodrigues Valente, Bruna Mattioly Oliveira, Antonio Edson Rocha Polidoro, Rafael Barbosa Zuccherato, Luciana Werneck Bartholomeu, Daniella Castanheira Schenkman, Sergio Gazzinelli, Ricardo Tostes Burleigh, Barbara A El-Sayed, Najib M. Teixeira, Santuza Maria Ribeiro |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Belew, Ashton Trey Giusta, Caroline Junqueira Luiz, Gabriela Flavia Rodrigues Valente, Bruna Mattioly Oliveira, Antonio Edson Rocha Polidoro, Rafael Barbosa Zuccherato, Luciana Werneck Bartholomeu, Daniella Castanheira Schenkman, Sergio Gazzinelli, Ricardo Tostes Burleigh, Barbara A El-Sayed, Najib M. Teixeira, Santuza Maria Ribeiro |
| dc.subject.other.pt_BR.fl_str_mv |
Amastigotas Trypanosoma cruzi |
| topic |
Amastigotas Trypanosoma cruzi Amastigotes Trypomastigotes Gene expression Parasitic diseases Trypanosoma cruzi Cell differentiation Epimastigotes Comparative genomic |
| dc.subject.en.pt_BR.fl_str_mv |
Amastigotes Trypomastigotes Gene expression Parasitic diseases Trypanosoma cruzi Cell differentiation Epimastigotes Comparative genomic |
| description |
University of Maryland. College Park. Department of Cell Biology and Molecular Genetics and Center for Bioinformatics and Computational Biology. Maryland, United States of America |
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2017 |
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2017 |
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2018-03-07T17:07:07Z |
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2018-03-07T17:07:07Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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BELEW, Ashton Trey et al. Comparative transcriptome profiling of virulent and non-virulent Trypanosoma cruzi underlines the role of surface proteins during infection. PLoS Pathog., v. 13, n. 12, art. e1006767, 2017. |
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https://www.arca.fiocruz.br/handle/icict/25171 |
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1553-7366 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.ppat.1006767 |
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BELEW, Ashton Trey et al. Comparative transcriptome profiling of virulent and non-virulent Trypanosoma cruzi underlines the role of surface proteins during infection. PLoS Pathog., v. 13, n. 12, art. e1006767, 2017. 1553-7366 10.1371/journal.ppat.1006767 |
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