Análise nanoestrutural da ação do ultra-som terapêutico sobre o processo de regeneração do tendão de ratos

Detalhes bibliográficos
Autor(a) principal: Frasson, Natália Figueiredo Viana
Data de Publicação: 2009
Outros Autores: Taciro, Charles, Parizotto, Nivaldo Antonio
Tipo de documento: Artigo
Idioma: por
Título da fonte: Fisioterapia e Pesquisa
Texto Completo: https://www.revistas.usp.br/fpusp/article/view/12133
Resumo: Therapeutic pulsed ultrasound (TPUS) is one among several therapies used to promote tissue repair. This study assessed the effect of TPUS at two different intensities on rat Achilles tendon's microstructure. Twenty four male adult Wistar rats (aged 6 months) were submitted to right calcanean tendon radical tenotomy, with no ensuing tendon suture, and divided into four groups: control group (GC) did not receive any treatment; group 1 (G1) was treated with 20%, 1 MHz TPUS at 0.3 W/cm²;, with effective radiation area of 1,0 cm²; group 2 (G2) was treated with same parameter TPUS, but at 1.5 W/cm² intensity; group 3 (G3) received simulated treatment (equipment off). In all three groups, treatment consisted of one daily 5-minute session, for ten days. On the 11th post-operative day animals were euthanized and their tendons removed for qualitative and quantitative analysis by polarized light microscopy (PLM), light microscopy (LM), and atomic force microscopy (AFM). Both PLM and AFM analysis results showed better collagen fibre organization, aggregation and orientation at G1. The quantitative analysis of fibroblasts (mean 400.7 fibroblast/field) and capillary vessels (mean 2.22 vessels/field) through LM showed no statistically significant differences between groups (p>;0.05). It may hence be said that the process of tendon regeneration in adult rats may benefit from treatment with TPUS at low intensity.
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spelling Análise nanoestrutural da ação do ultra-som terapêutico sobre o processo de regeneração do tendão de ratos Nanostructural analysis of therapeutic ultrasound effect on rat tendon healing process RatosTendão do calcâneo^i1^sreabilitaTerapia por ultra-somAchilles tendon^i2^srehabilitatRatsUltrasonic therapy Therapeutic pulsed ultrasound (TPUS) is one among several therapies used to promote tissue repair. This study assessed the effect of TPUS at two different intensities on rat Achilles tendon's microstructure. Twenty four male adult Wistar rats (aged 6 months) were submitted to right calcanean tendon radical tenotomy, with no ensuing tendon suture, and divided into four groups: control group (GC) did not receive any treatment; group 1 (G1) was treated with 20%, 1 MHz TPUS at 0.3 W/cm²;, with effective radiation area of 1,0 cm²; group 2 (G2) was treated with same parameter TPUS, but at 1.5 W/cm² intensity; group 3 (G3) received simulated treatment (equipment off). In all three groups, treatment consisted of one daily 5-minute session, for ten days. On the 11th post-operative day animals were euthanized and their tendons removed for qualitative and quantitative analysis by polarized light microscopy (PLM), light microscopy (LM), and atomic force microscopy (AFM). Both PLM and AFM analysis results showed better collagen fibre organization, aggregation and orientation at G1. The quantitative analysis of fibroblasts (mean 400.7 fibroblast/field) and capillary vessels (mean 2.22 vessels/field) through LM showed no statistically significant differences between groups (p>;0.05). It may hence be said that the process of tendon regeneration in adult rats may benefit from treatment with TPUS at low intensity. O ultra-som terapêutico pulsado (USTP) é uma das modalidades terapêuticas utilizadas para promover a aceleração do reparo tendíneo. Este estudo avaliou a microestrutura do tendão calcâneo sob o efeito do USTP em duas intensidades diferentes. Foram utilizados 24 ratos Wistar machos, divididos em quatro grupos, submetidos à tenotomia radical transversal do tendão calcâneo direito, sem posterior tenorrafia. Os animais do grupo controle (GC) não receberam tratamento; o grupo 1 (G1) foi submetido ao USTP de 1 MHz a 20%, com área de radiação efetiva de 1,0 cm² e intensidade média instantânea de 0,3 W/cm²; o grupo 2 (G2) recebeu USTP nos mesmos parãmetros, mas com intensidade de 1,5 W/cm²; no grupo 3 (G3), placebo, foi aplicado tratamento simulado (ultra-som desligado). Nos três grupos, o tratamento consistiu em uma sessão diária de 5 minutos, por 10 dias. No 11º dia pós-operatório os tendões foram removidos para análise qualitativa e quantitativa, por meio de microscopia de luz polarizada (MLP), de luz (ML) e de força atômica (MFA). As análises qualitativas de MLP e MFA foram coincidentes, mostrando melhor organização, agregação e orientação das fibras de colágeno no grupo G1. A análise quantitativa apresentou contagens médias de 400,7 fibroblastos e 2,22 capilares sangüíneos por campo de análise, não se encontrando diferença significativa entre os grupos (p>;0,05). Conclui-se que o processo de regeneração tendínea em ratos pode ser beneficiado pelo tratamento com USTP em baixa intensidade. Universidade de São Paulo. Faculdade de Medicina2009-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/fpusp/article/view/1213310.1590/S1809-29502009000300002Fisioterapia e Pesquisa; Vol. 16 No. 3 (2009); 198-204 Fisioterapia e Pesquisa; Vol. 16 Núm. 3 (2009); 198-204 Fisioterapia e Pesquisa; v. 16 n. 3 (2009); 198-204 2316-91171809-2950reponame:Fisioterapia e Pesquisainstname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/fpusp/article/view/12133/13910Copyright (c) 2017 Fisioterapia e Pesquisainfo:eu-repo/semantics/openAccessFrasson, Natália Figueiredo VianaTaciro, CharlesParizotto, Nivaldo Antonio2012-05-13T15:59:20Zoai:revistas.usp.br:article/12133Revistahttp://www.revistas.usp.br/fpuspPUBhttps://www.revistas.usp.br/fpusp/oai||revfisio@usp.br2316-91171809-2950opendoar:2012-05-13T15:59:20Fisioterapia e Pesquisa - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Análise nanoestrutural da ação do ultra-som terapêutico sobre o processo de regeneração do tendão de ratos
Nanostructural analysis of therapeutic ultrasound effect on rat tendon healing process
title Análise nanoestrutural da ação do ultra-som terapêutico sobre o processo de regeneração do tendão de ratos
spellingShingle Análise nanoestrutural da ação do ultra-som terapêutico sobre o processo de regeneração do tendão de ratos
Frasson, Natália Figueiredo Viana
Ratos
Tendão do calcâneo^i1^sreabilita
Terapia por ultra-som
Achilles tendon^i2^srehabilitat
Rats
Ultrasonic therapy
title_short Análise nanoestrutural da ação do ultra-som terapêutico sobre o processo de regeneração do tendão de ratos
title_full Análise nanoestrutural da ação do ultra-som terapêutico sobre o processo de regeneração do tendão de ratos
title_fullStr Análise nanoestrutural da ação do ultra-som terapêutico sobre o processo de regeneração do tendão de ratos
title_full_unstemmed Análise nanoestrutural da ação do ultra-som terapêutico sobre o processo de regeneração do tendão de ratos
title_sort Análise nanoestrutural da ação do ultra-som terapêutico sobre o processo de regeneração do tendão de ratos
author Frasson, Natália Figueiredo Viana
author_facet Frasson, Natália Figueiredo Viana
Taciro, Charles
Parizotto, Nivaldo Antonio
author_role author
author2 Taciro, Charles
Parizotto, Nivaldo Antonio
author2_role author
author
dc.contributor.author.fl_str_mv Frasson, Natália Figueiredo Viana
Taciro, Charles
Parizotto, Nivaldo Antonio
dc.subject.por.fl_str_mv Ratos
Tendão do calcâneo^i1^sreabilita
Terapia por ultra-som
Achilles tendon^i2^srehabilitat
Rats
Ultrasonic therapy
topic Ratos
Tendão do calcâneo^i1^sreabilita
Terapia por ultra-som
Achilles tendon^i2^srehabilitat
Rats
Ultrasonic therapy
description Therapeutic pulsed ultrasound (TPUS) is one among several therapies used to promote tissue repair. This study assessed the effect of TPUS at two different intensities on rat Achilles tendon's microstructure. Twenty four male adult Wistar rats (aged 6 months) were submitted to right calcanean tendon radical tenotomy, with no ensuing tendon suture, and divided into four groups: control group (GC) did not receive any treatment; group 1 (G1) was treated with 20%, 1 MHz TPUS at 0.3 W/cm²;, with effective radiation area of 1,0 cm²; group 2 (G2) was treated with same parameter TPUS, but at 1.5 W/cm² intensity; group 3 (G3) received simulated treatment (equipment off). In all three groups, treatment consisted of one daily 5-minute session, for ten days. On the 11th post-operative day animals were euthanized and their tendons removed for qualitative and quantitative analysis by polarized light microscopy (PLM), light microscopy (LM), and atomic force microscopy (AFM). Both PLM and AFM analysis results showed better collagen fibre organization, aggregation and orientation at G1. The quantitative analysis of fibroblasts (mean 400.7 fibroblast/field) and capillary vessels (mean 2.22 vessels/field) through LM showed no statistically significant differences between groups (p>;0.05). It may hence be said that the process of tendon regeneration in adult rats may benefit from treatment with TPUS at low intensity.
publishDate 2009
dc.date.none.fl_str_mv 2009-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/fpusp/article/view/12133
10.1590/S1809-29502009000300002
url https://www.revistas.usp.br/fpusp/article/view/12133
identifier_str_mv 10.1590/S1809-29502009000300002
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/fpusp/article/view/12133/13910
dc.rights.driver.fl_str_mv Copyright (c) 2017 Fisioterapia e Pesquisa
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Fisioterapia e Pesquisa
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina
dc.source.none.fl_str_mv Fisioterapia e Pesquisa; Vol. 16 No. 3 (2009); 198-204
Fisioterapia e Pesquisa; Vol. 16 Núm. 3 (2009); 198-204
Fisioterapia e Pesquisa; v. 16 n. 3 (2009); 198-204
2316-9117
1809-2950
reponame:Fisioterapia e Pesquisa
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Fisioterapia e Pesquisa
collection Fisioterapia e Pesquisa
repository.name.fl_str_mv Fisioterapia e Pesquisa - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||revfisio@usp.br
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