Análise nanoestrutural da ação do ultra-som terapêutico sobre o processo de regeneração do tendão de ratos
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Fisioterapia e Pesquisa |
Texto Completo: | https://www.revistas.usp.br/fpusp/article/view/12133 |
Resumo: | Therapeutic pulsed ultrasound (TPUS) is one among several therapies used to promote tissue repair. This study assessed the effect of TPUS at two different intensities on rat Achilles tendon's microstructure. Twenty four male adult Wistar rats (aged 6 months) were submitted to right calcanean tendon radical tenotomy, with no ensuing tendon suture, and divided into four groups: control group (GC) did not receive any treatment; group 1 (G1) was treated with 20%, 1 MHz TPUS at 0.3 W/cm²;, with effective radiation area of 1,0 cm²; group 2 (G2) was treated with same parameter TPUS, but at 1.5 W/cm² intensity; group 3 (G3) received simulated treatment (equipment off). In all three groups, treatment consisted of one daily 5-minute session, for ten days. On the 11th post-operative day animals were euthanized and their tendons removed for qualitative and quantitative analysis by polarized light microscopy (PLM), light microscopy (LM), and atomic force microscopy (AFM). Both PLM and AFM analysis results showed better collagen fibre organization, aggregation and orientation at G1. The quantitative analysis of fibroblasts (mean 400.7 fibroblast/field) and capillary vessels (mean 2.22 vessels/field) through LM showed no statistically significant differences between groups (p>;0.05). It may hence be said that the process of tendon regeneration in adult rats may benefit from treatment with TPUS at low intensity. |
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Análise nanoestrutural da ação do ultra-som terapêutico sobre o processo de regeneração do tendão de ratos Nanostructural analysis of therapeutic ultrasound effect on rat tendon healing process RatosTendão do calcâneo^i1^sreabilitaTerapia por ultra-somAchilles tendon^i2^srehabilitatRatsUltrasonic therapy Therapeutic pulsed ultrasound (TPUS) is one among several therapies used to promote tissue repair. This study assessed the effect of TPUS at two different intensities on rat Achilles tendon's microstructure. Twenty four male adult Wistar rats (aged 6 months) were submitted to right calcanean tendon radical tenotomy, with no ensuing tendon suture, and divided into four groups: control group (GC) did not receive any treatment; group 1 (G1) was treated with 20%, 1 MHz TPUS at 0.3 W/cm²;, with effective radiation area of 1,0 cm²; group 2 (G2) was treated with same parameter TPUS, but at 1.5 W/cm² intensity; group 3 (G3) received simulated treatment (equipment off). In all three groups, treatment consisted of one daily 5-minute session, for ten days. On the 11th post-operative day animals were euthanized and their tendons removed for qualitative and quantitative analysis by polarized light microscopy (PLM), light microscopy (LM), and atomic force microscopy (AFM). Both PLM and AFM analysis results showed better collagen fibre organization, aggregation and orientation at G1. The quantitative analysis of fibroblasts (mean 400.7 fibroblast/field) and capillary vessels (mean 2.22 vessels/field) through LM showed no statistically significant differences between groups (p>;0.05). It may hence be said that the process of tendon regeneration in adult rats may benefit from treatment with TPUS at low intensity. O ultra-som terapêutico pulsado (USTP) é uma das modalidades terapêuticas utilizadas para promover a aceleração do reparo tendíneo. Este estudo avaliou a microestrutura do tendão calcâneo sob o efeito do USTP em duas intensidades diferentes. Foram utilizados 24 ratos Wistar machos, divididos em quatro grupos, submetidos à tenotomia radical transversal do tendão calcâneo direito, sem posterior tenorrafia. Os animais do grupo controle (GC) não receberam tratamento; o grupo 1 (G1) foi submetido ao USTP de 1 MHz a 20%, com área de radiação efetiva de 1,0 cm² e intensidade média instantânea de 0,3 W/cm²; o grupo 2 (G2) recebeu USTP nos mesmos parãmetros, mas com intensidade de 1,5 W/cm²; no grupo 3 (G3), placebo, foi aplicado tratamento simulado (ultra-som desligado). Nos três grupos, o tratamento consistiu em uma sessão diária de 5 minutos, por 10 dias. No 11º dia pós-operatório os tendões foram removidos para análise qualitativa e quantitativa, por meio de microscopia de luz polarizada (MLP), de luz (ML) e de força atômica (MFA). As análises qualitativas de MLP e MFA foram coincidentes, mostrando melhor organização, agregação e orientação das fibras de colágeno no grupo G1. A análise quantitativa apresentou contagens médias de 400,7 fibroblastos e 2,22 capilares sangüíneos por campo de análise, não se encontrando diferença significativa entre os grupos (p>;0,05). Conclui-se que o processo de regeneração tendínea em ratos pode ser beneficiado pelo tratamento com USTP em baixa intensidade. Universidade de São Paulo. Faculdade de Medicina2009-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/fpusp/article/view/1213310.1590/S1809-29502009000300002Fisioterapia e Pesquisa; Vol. 16 No. 3 (2009); 198-204 Fisioterapia e Pesquisa; Vol. 16 Núm. 3 (2009); 198-204 Fisioterapia e Pesquisa; v. 16 n. 3 (2009); 198-204 2316-91171809-2950reponame:Fisioterapia e Pesquisainstname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/fpusp/article/view/12133/13910Copyright (c) 2017 Fisioterapia e Pesquisainfo:eu-repo/semantics/openAccessFrasson, Natália Figueiredo VianaTaciro, CharlesParizotto, Nivaldo Antonio2012-05-13T15:59:20Zoai:revistas.usp.br:article/12133Revistahttp://www.revistas.usp.br/fpuspPUBhttps://www.revistas.usp.br/fpusp/oai||revfisio@usp.br2316-91171809-2950opendoar:2012-05-13T15:59:20Fisioterapia e Pesquisa - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Análise nanoestrutural da ação do ultra-som terapêutico sobre o processo de regeneração do tendão de ratos Nanostructural analysis of therapeutic ultrasound effect on rat tendon healing process |
title |
Análise nanoestrutural da ação do ultra-som terapêutico sobre o processo de regeneração do tendão de ratos |
spellingShingle |
Análise nanoestrutural da ação do ultra-som terapêutico sobre o processo de regeneração do tendão de ratos Frasson, Natália Figueiredo Viana Ratos Tendão do calcâneo^i1^sreabilita Terapia por ultra-som Achilles tendon^i2^srehabilitat Rats Ultrasonic therapy |
title_short |
Análise nanoestrutural da ação do ultra-som terapêutico sobre o processo de regeneração do tendão de ratos |
title_full |
Análise nanoestrutural da ação do ultra-som terapêutico sobre o processo de regeneração do tendão de ratos |
title_fullStr |
Análise nanoestrutural da ação do ultra-som terapêutico sobre o processo de regeneração do tendão de ratos |
title_full_unstemmed |
Análise nanoestrutural da ação do ultra-som terapêutico sobre o processo de regeneração do tendão de ratos |
title_sort |
Análise nanoestrutural da ação do ultra-som terapêutico sobre o processo de regeneração do tendão de ratos |
author |
Frasson, Natália Figueiredo Viana |
author_facet |
Frasson, Natália Figueiredo Viana Taciro, Charles Parizotto, Nivaldo Antonio |
author_role |
author |
author2 |
Taciro, Charles Parizotto, Nivaldo Antonio |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Frasson, Natália Figueiredo Viana Taciro, Charles Parizotto, Nivaldo Antonio |
dc.subject.por.fl_str_mv |
Ratos Tendão do calcâneo^i1^sreabilita Terapia por ultra-som Achilles tendon^i2^srehabilitat Rats Ultrasonic therapy |
topic |
Ratos Tendão do calcâneo^i1^sreabilita Terapia por ultra-som Achilles tendon^i2^srehabilitat Rats Ultrasonic therapy |
description |
Therapeutic pulsed ultrasound (TPUS) is one among several therapies used to promote tissue repair. This study assessed the effect of TPUS at two different intensities on rat Achilles tendon's microstructure. Twenty four male adult Wistar rats (aged 6 months) were submitted to right calcanean tendon radical tenotomy, with no ensuing tendon suture, and divided into four groups: control group (GC) did not receive any treatment; group 1 (G1) was treated with 20%, 1 MHz TPUS at 0.3 W/cm²;, with effective radiation area of 1,0 cm²; group 2 (G2) was treated with same parameter TPUS, but at 1.5 W/cm² intensity; group 3 (G3) received simulated treatment (equipment off). In all three groups, treatment consisted of one daily 5-minute session, for ten days. On the 11th post-operative day animals were euthanized and their tendons removed for qualitative and quantitative analysis by polarized light microscopy (PLM), light microscopy (LM), and atomic force microscopy (AFM). Both PLM and AFM analysis results showed better collagen fibre organization, aggregation and orientation at G1. The quantitative analysis of fibroblasts (mean 400.7 fibroblast/field) and capillary vessels (mean 2.22 vessels/field) through LM showed no statistically significant differences between groups (p>;0.05). It may hence be said that the process of tendon regeneration in adult rats may benefit from treatment with TPUS at low intensity. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/fpusp/article/view/12133 10.1590/S1809-29502009000300002 |
url |
https://www.revistas.usp.br/fpusp/article/view/12133 |
identifier_str_mv |
10.1590/S1809-29502009000300002 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/fpusp/article/view/12133/13910 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Fisioterapia e Pesquisa info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Fisioterapia e Pesquisa |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina |
dc.source.none.fl_str_mv |
Fisioterapia e Pesquisa; Vol. 16 No. 3 (2009); 198-204 Fisioterapia e Pesquisa; Vol. 16 Núm. 3 (2009); 198-204 Fisioterapia e Pesquisa; v. 16 n. 3 (2009); 198-204 2316-9117 1809-2950 reponame:Fisioterapia e Pesquisa instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Fisioterapia e Pesquisa |
collection |
Fisioterapia e Pesquisa |
repository.name.fl_str_mv |
Fisioterapia e Pesquisa - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||revfisio@usp.br |
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1787713737989292032 |