Expression of the junctional proteins connexin 43 and ZO-1 in differentiating ameloblasts, odontoblasts and osteoblasts in tooth germs and alveolar bone from rat molars

Detalhes bibliográficos
Autor(a) principal: João, Silvia Maria Amado
Data de Publicação: 2003
Tipo de documento: Artigo
Idioma: por
Título da fonte: Fisioterapia e Pesquisa
Texto Completo: https://www.revistas.usp.br/fpusp/article/view/78178
Resumo: We studied the distribution of Cx43 and ZO-1 by confocal laser scanning microscopy at early stages of amelogenesis and dentinogenesis in toothgerms and osteogenesis in alveolar bone from rats. Cx43 was detected at the distal and medial regions of undifferentiated ameloblasts, between cells from stratum intermedium and stellate reticulum. Differentiating odontoblasts exhibited immunoreaction at their distal end. ZO-1 was observed at proximal and distal junctional complexes of ameloblasts; in the odontoblasts was present at apical regions, with punctate appearance. In more advanced stages, expression of Cx43 was more intense on ameloblasts and odontoblasts in the same area. Immunoreaction for ZO-1 in ameloblasts was stronger than that the previous stage in ameloblasts, in their junctional complexes as well as in odontoblasts intheir distal area. In the alveolar bone, Cx43 was present in osteoblasts and osteocytes and/or cell processes. The ZO-1 was detected in the osteoblast surface. This study showed that differentiating ameloblasts, odontoblasts and osteoblasts start to express Cx43 and ZO-1 as early astheir differentiation process begins.
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spelling Expression of the junctional proteins connexin 43 and ZO-1 in differentiating ameloblasts, odontoblasts and osteoblasts in tooth germs and alveolar bone from rat molarsExpressão das proteínas juncionais Conexina- 43 e ZO-1, em ameloblastos, odontoblasts e osteoblastos em diferenciação, em germes dentários de molares e osso alveolar de ratosamelogênesedentinogênesemicroscopia confocal/métodososteoblastosratos.amelogenesisdentinogenesismicroscopyconfocal/methodsosteoblastsrats.We studied the distribution of Cx43 and ZO-1 by confocal laser scanning microscopy at early stages of amelogenesis and dentinogenesis in toothgerms and osteogenesis in alveolar bone from rats. Cx43 was detected at the distal and medial regions of undifferentiated ameloblasts, between cells from stratum intermedium and stellate reticulum. Differentiating odontoblasts exhibited immunoreaction at their distal end. ZO-1 was observed at proximal and distal junctional complexes of ameloblasts; in the odontoblasts was present at apical regions, with punctate appearance. In more advanced stages, expression of Cx43 was more intense on ameloblasts and odontoblasts in the same area. Immunoreaction for ZO-1 in ameloblasts was stronger than that the previous stage in ameloblasts, in their junctional complexes as well as in odontoblasts intheir distal area. In the alveolar bone, Cx43 was present in osteoblasts and osteocytes and/or cell processes. The ZO-1 was detected in the osteoblast surface. This study showed that differentiating ameloblasts, odontoblasts and osteoblasts start to express Cx43 and ZO-1 as early astheir differentiation process begins.Foi estudada a distribuição da Cx43 e ZO-1 através da microscopia confocal de varredura a laser nos estágios iniciais da amelogênese e dentinogênese em germes dentários de molares e osteogênese no osso alveolar, de ratos. A Cx43 foi detectada nas regiões distais e laterais dosameloblastos indiferenciados, entre células do estrato intermediário e reticulo estrelado, enquanto que nos odontoblastos em diferenciação a imunorreatividade apareceu na sua porção distai. A ZO-1 foi observada nos complexos juncionais proximal e distal dos ameloblastos; nos odontoblastos, esta foi observada nas regiões apicais, com uma aparência pontual. Nos estágios mais avançados, a expressão da Cx43 nos ameloblastos e odontoblastos foi detectadanas mesmas regiões, porém mais intensa. A imunorreação para ZO-1 nos ameloblastos foi mais intensa nos estágios avançados tanto em ameloblastos, na região dos complexos juncionais como nos odontoblastos na porção distai. No osso alveolar, a Cx43 estava presente nos osteoblastos e nos osteócitos e/ou prolongamentos, enquanto a ZO-1 foi detectada nas superfícies dos osteoblastos. Este estudo mostrou que os ameloblastos, odontoblastos e osteoblastos em diferenciação expressam Cx43 e ZO-1 desde o início de seu processo de diferenciação.Universidade de São Paulo. Faculdade de Medicina2003-12-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/fpusp/article/view/7817810.1590/fpusp.v10i2.78178Fisioterapia e Pesquisa; Vol. 10 No. 2 (2003); 102-102Fisioterapia e Pesquisa; Vol. 10 Núm. 2 (2003); 102-102Fisioterapia e Pesquisa; v. 10 n. 2 (2003); 102-1022316-91171809-2950reponame:Fisioterapia e Pesquisainstname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/fpusp/article/view/78178/82259Copyright (c) 2017 Fisioterapia e Pesquisainfo:eu-repo/semantics/openAccessJoão, Silvia Maria Amado2014-05-07T22:41:18Zoai:revistas.usp.br:article/78178Revistahttp://www.revistas.usp.br/fpuspPUBhttps://www.revistas.usp.br/fpusp/oai||revfisio@usp.br2316-91171809-2950opendoar:2014-05-07T22:41:18Fisioterapia e Pesquisa - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Expression of the junctional proteins connexin 43 and ZO-1 in differentiating ameloblasts, odontoblasts and osteoblasts in tooth germs and alveolar bone from rat molars
Expressão das proteínas juncionais Conexina- 43 e ZO-1, em ameloblastos, odontoblasts e osteoblastos em diferenciação, em germes dentários de molares e osso alveolar de ratos
title Expression of the junctional proteins connexin 43 and ZO-1 in differentiating ameloblasts, odontoblasts and osteoblasts in tooth germs and alveolar bone from rat molars
spellingShingle Expression of the junctional proteins connexin 43 and ZO-1 in differentiating ameloblasts, odontoblasts and osteoblasts in tooth germs and alveolar bone from rat molars
João, Silvia Maria Amado
amelogênese
dentinogênese
microscopia confocal/métodos
osteoblastos
ratos.
amelogenesis
dentinogenesis
microscopy
confocal/methods
osteoblasts
rats.
title_short Expression of the junctional proteins connexin 43 and ZO-1 in differentiating ameloblasts, odontoblasts and osteoblasts in tooth germs and alveolar bone from rat molars
title_full Expression of the junctional proteins connexin 43 and ZO-1 in differentiating ameloblasts, odontoblasts and osteoblasts in tooth germs and alveolar bone from rat molars
title_fullStr Expression of the junctional proteins connexin 43 and ZO-1 in differentiating ameloblasts, odontoblasts and osteoblasts in tooth germs and alveolar bone from rat molars
title_full_unstemmed Expression of the junctional proteins connexin 43 and ZO-1 in differentiating ameloblasts, odontoblasts and osteoblasts in tooth germs and alveolar bone from rat molars
title_sort Expression of the junctional proteins connexin 43 and ZO-1 in differentiating ameloblasts, odontoblasts and osteoblasts in tooth germs and alveolar bone from rat molars
author João, Silvia Maria Amado
author_facet João, Silvia Maria Amado
author_role author
dc.contributor.author.fl_str_mv João, Silvia Maria Amado
dc.subject.por.fl_str_mv amelogênese
dentinogênese
microscopia confocal/métodos
osteoblastos
ratos.
amelogenesis
dentinogenesis
microscopy
confocal/methods
osteoblasts
rats.
topic amelogênese
dentinogênese
microscopia confocal/métodos
osteoblastos
ratos.
amelogenesis
dentinogenesis
microscopy
confocal/methods
osteoblasts
rats.
description We studied the distribution of Cx43 and ZO-1 by confocal laser scanning microscopy at early stages of amelogenesis and dentinogenesis in toothgerms and osteogenesis in alveolar bone from rats. Cx43 was detected at the distal and medial regions of undifferentiated ameloblasts, between cells from stratum intermedium and stellate reticulum. Differentiating odontoblasts exhibited immunoreaction at their distal end. ZO-1 was observed at proximal and distal junctional complexes of ameloblasts; in the odontoblasts was present at apical regions, with punctate appearance. In more advanced stages, expression of Cx43 was more intense on ameloblasts and odontoblasts in the same area. Immunoreaction for ZO-1 in ameloblasts was stronger than that the previous stage in ameloblasts, in their junctional complexes as well as in odontoblasts intheir distal area. In the alveolar bone, Cx43 was present in osteoblasts and osteocytes and/or cell processes. The ZO-1 was detected in the osteoblast surface. This study showed that differentiating ameloblasts, odontoblasts and osteoblasts start to express Cx43 and ZO-1 as early astheir differentiation process begins.
publishDate 2003
dc.date.none.fl_str_mv 2003-12-31
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/fpusp/article/view/78178
10.1590/fpusp.v10i2.78178
url https://www.revistas.usp.br/fpusp/article/view/78178
identifier_str_mv 10.1590/fpusp.v10i2.78178
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/fpusp/article/view/78178/82259
dc.rights.driver.fl_str_mv Copyright (c) 2017 Fisioterapia e Pesquisa
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Fisioterapia e Pesquisa
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina
dc.source.none.fl_str_mv Fisioterapia e Pesquisa; Vol. 10 No. 2 (2003); 102-102
Fisioterapia e Pesquisa; Vol. 10 Núm. 2 (2003); 102-102
Fisioterapia e Pesquisa; v. 10 n. 2 (2003); 102-102
2316-9117
1809-2950
reponame:Fisioterapia e Pesquisa
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Fisioterapia e Pesquisa
collection Fisioterapia e Pesquisa
repository.name.fl_str_mv Fisioterapia e Pesquisa - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||revfisio@usp.br
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