Unconsciousness assessment through electroencephalography (EEG), in the process of stunning for humane slaughter of Nile tilapia
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Tese |
Idioma: | eng |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da USP |
Texto Completo: | https://www.teses.usp.br/teses/disponiveis/74/74131/tde-30042021-121501/ |
Resumo: | Chapter 1 presents a brief introduction to the subject and the aim of these. Chapter 2 assessed Ocimum americanum (OA) or Lippia alba (LA) essential oils as anaesthetics in Nile tilapia and their influence on the aroma. For the anaesthetic evaluation, Nile tilapia were divided into four groups: ethanol (ET), 2-phenoxyethanol (PE), Ocimum americanum (OA) or Lippia alba (LA), all at 500 µL/L. OA or LA essential oils at 500 µL/L were effective in inducing deep anaesthesia with unconsciousness duration of 126 s, allowing time for bleeding. Anaesthesia with Ocimum americanum or Lippia alba essential oils may be indicated as an alternative to hypothermia at pre-slaughter handling of fish in Brazil. Chapter 3 aimed to assess mechanical spiking and electrical stunning as methods to induce unconsciousness in Nile tilapia, and their effects on rigor mortis. Nile tilapia were divided into three groups, two groups for spiking and one group for electrical stunning. Mechanical spiking with lateral access is effective to induce unconsciousness without recovery, delaying the onset of rigor mortis in Nile tilapia, thus its use in an automatic system could be an alternative to traditional slaughter of hypothermia in Brazil. Chapter 4 aimed to assess unconsciousness using electroencephalography EEG in Nile tilapia under different methods at pre-slaughter. Nile tilapia were divided in five different methods at pre-slaughter: anaesthesia with phenoxyethanol at 1 mL/L (PE), anaesthesia with Ocimum americanum (OA) or Lippia alba (LA) essential oils at 500 µL/L, spiking (SP) and hypothermia in ice/water (2:1). The median time (s) to lose consciousness as represented by the suppressed phase was PE (136 ± 61), OA (171 ± 164), LA (171.5 ±77.8) and HP (252 ± 752) (P<0.01). Spectral analysis suggested unconsciousness after 3 minutes of exposure to PE, OA or LA due to the decreases in Ptot, F50 and F95, and also the increase in contribution from the delta frequency and decrease in contribution from the beta frequency. The values obtained in the spectral analysis for fish submitted to HP suggest that this method does not induce unconsciousness. When used in Nile tilapia, phenoxyethanol-like anaesthetic showed both EEG traces and spectral analyses consistent with unconsciousness after 140 s of immersion. Anaesthesia with 500µL/L of the essential oils Ocimum americanum or Lippia alba induced unconsciousness after 180 s of immersion, as determined by EEG. The use of hypothermia at pre-slaughter of Nile tilapia does not induce unconsciousness and should be urgently substituted by methods that meet criteria of humane slaughter. Chapter 5 assessed the influence of different pre-slaughter methods: hypothermia live chilling (HP); anaesthesia with the essential oils Ocimum americanum (OA) or Lippia alba (LA) and mechanical spiking (SP) on the meat quality parameters of Nile tilapia stored under refrigeration for 15 days. The use of mechanical spiking and anaesthesia with the Ocimum americanum (OA) or Lippia alba (LA) essential oils in Nile tilapia did not negatively change the following quality parameters of the meat: pH value, rigor mortis, TVB-N and K-value as compared to the traditional method of hypothermia. |
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Unconsciousness assessment through electroencephalography (EEG), in the process of stunning for humane slaughter of Nile tilapiaAvaliação de inconsciência, por meio de eletroencefalografia (EEG), no processo de insensibilização para abate humanitário de tilápia do NiloAbateAnaesthesiaAnestesiaAnimal welfareBehaviour indicatorsBem-estar animalBem-estar de peixesEssential oilsFish welfareHipotermiaHypothermiaIndicadores de comportamentoInsensibilizaçãoMeat qualityÓleos essenciaisQualidade da carneSlaughterStunningUnconsciousnessChapter 1 presents a brief introduction to the subject and the aim of these. Chapter 2 assessed Ocimum americanum (OA) or Lippia alba (LA) essential oils as anaesthetics in Nile tilapia and their influence on the aroma. For the anaesthetic evaluation, Nile tilapia were divided into four groups: ethanol (ET), 2-phenoxyethanol (PE), Ocimum americanum (OA) or Lippia alba (LA), all at 500 µL/L. OA or LA essential oils at 500 µL/L were effective in inducing deep anaesthesia with unconsciousness duration of 126 s, allowing time for bleeding. Anaesthesia with Ocimum americanum or Lippia alba essential oils may be indicated as an alternative to hypothermia at pre-slaughter handling of fish in Brazil. Chapter 3 aimed to assess mechanical spiking and electrical stunning as methods to induce unconsciousness in Nile tilapia, and their effects on rigor mortis. Nile tilapia were divided into three groups, two groups for spiking and one group for electrical stunning. Mechanical spiking with lateral access is effective to induce unconsciousness without recovery, delaying the onset of rigor mortis in Nile tilapia, thus its use in an automatic system could be an alternative to traditional slaughter of hypothermia in Brazil. Chapter 4 aimed to assess unconsciousness using electroencephalography EEG in Nile tilapia under different methods at pre-slaughter. Nile tilapia were divided in five different methods at pre-slaughter: anaesthesia with phenoxyethanol at 1 mL/L (PE), anaesthesia with Ocimum americanum (OA) or Lippia alba (LA) essential oils at 500 µL/L, spiking (SP) and hypothermia in ice/water (2:1). The median time (s) to lose consciousness as represented by the suppressed phase was PE (136 ± 61), OA (171 ± 164), LA (171.5 ±77.8) and HP (252 ± 752) (P<0.01). Spectral analysis suggested unconsciousness after 3 minutes of exposure to PE, OA or LA due to the decreases in Ptot, F50 and F95, and also the increase in contribution from the delta frequency and decrease in contribution from the beta frequency. The values obtained in the spectral analysis for fish submitted to HP suggest that this method does not induce unconsciousness. When used in Nile tilapia, phenoxyethanol-like anaesthetic showed both EEG traces and spectral analyses consistent with unconsciousness after 140 s of immersion. Anaesthesia with 500µL/L of the essential oils Ocimum americanum or Lippia alba induced unconsciousness after 180 s of immersion, as determined by EEG. The use of hypothermia at pre-slaughter of Nile tilapia does not induce unconsciousness and should be urgently substituted by methods that meet criteria of humane slaughter. Chapter 5 assessed the influence of different pre-slaughter methods: hypothermia live chilling (HP); anaesthesia with the essential oils Ocimum americanum (OA) or Lippia alba (LA) and mechanical spiking (SP) on the meat quality parameters of Nile tilapia stored under refrigeration for 15 days. The use of mechanical spiking and anaesthesia with the Ocimum americanum (OA) or Lippia alba (LA) essential oils in Nile tilapia did not negatively change the following quality parameters of the meat: pH value, rigor mortis, TVB-N and K-value as compared to the traditional method of hypothermia.No Capítulo 1 apresenta-se uma breve introdução ao assunto, assim como os objetivos da tese. No Capítulo 2 o objetivo foi avaliar os óleos essenciais de Ocimum americanum (OA) ou Lippia alba (LA) como anestésicos para tilápia do Nilo, assim como a influência dos mesmos no aroma e sabor dos filés. Para a avaliação anestésica, tilápias do Nilo foram divididas em 4 grupos: etanol (ET), 2-fenoxietanol (PE) e óleos essenciais de Ocimum americanum (OA) ou Lippia alba (LA). Óleos essenciais de OA ou LA a 500 µL/L foram efetivos para indução de anestesia profunda com uma duração da inconsciência que permitiu a sangria. Anestesia com óleos essenciais de Ocimum americanum ou Lippia alba podem ser indicados como uma alternativa à hipotermia no pré-abate de peixes no Brasil. No Capítulo 3 o objetivo foi avaliar a percussão mecânica perfurante Spiking e a eletronarcose como métodos para indução de inconsciência, e seus efeitos sobre o rigor mortis. Tilápias do Nilo foram divididas em três grupos, dois para percussão mecânica e um grupo para eletronarcose. A percussão mecânica com acesso lateral foi efetiva para induzir inconsciência sem recuperação em tilápia do Nilo, e seu uso em sistema automatizado pode ser uma alternativa ao método tradicional de hipotermia no Brasil. No Capítulo 4 foi avaliada a inconsciência por meio de eletroencefalografia (EEG) em tilápias do Nilo submetidas a diferentes métodos ao pré-abate. Tilápias do Nilo foram divididas em cinco diferentes grupos para avaliar métodos no período pré-abate, anestesia com 2-fenoxietanol 1 ml/L (PE), anestesia com óleos essências de Ocimum americanum (OA) ou Lippia alba a 500 µL/L, spiking (SP) e hipotermia (HP). A mediana de tempo (s) para perder a consciência representada pela fase suprimida foi PE (136 ± 61), OA (171 ± 164), LA (171.5 ±77.8) e HP (252 ± 752) (P<0.01). A análise espectral sugere inconsciência após 3 minutos de exposição aos anestésicos PE, OA, LA devido à diminuição de Ptot, F50 e F95 e incremento na contribuição de frequências delta e diminuição nas frequências de beta. Os valores de HP sugerem que tal método não induz inconsciência. Anestesia com Ocimum americanum ou Lippia alba induz inconsciência determinada por EEG após 180 s de imersão. O uso da hipotermia não induz inconsciência em tilápia do Nilo e não cumpre com critérios de abate humanitário, sendo urgente sua substituição por métodos que cumpram critérios de abate humanitário. No Capítulo 5 o objetivo foi avaliar a influência de diferentes métodos pré-abate: hipotermia (HP), anestesia com os óleos essências de Ocimum americanum (OA) ou Lippia alba e spiking (SP) sobre parâmetros da qualidade da carne de tilápia sem evisceração e refrigerada durante 15 dias. O uso da percussão mecânica spiking (SP) e anestesia com óleos essenciais de Ocimum americanum (OA) ou Lippia alba (LA) em tilápia do Nilo sob refrigeração não alterou negativamente os seguintes parâmetros de qualidade da carne pH, rigor mortis, TVB-N e valor K quando comparado ao método tradicional de hipotermia.Biblioteca Digitais de Teses e Dissertações da USPViegas, Elisabete Maria MacedoRucinque, Daniel Santiago2021-01-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttps://www.teses.usp.br/teses/disponiveis/74/74131/tde-30042021-121501/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2022-03-30T14:18:15Zoai:teses.usp.br:tde-30042021-121501Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212022-03-30T14:18:15Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Unconsciousness assessment through electroencephalography (EEG), in the process of stunning for humane slaughter of Nile tilapia Avaliação de inconsciência, por meio de eletroencefalografia (EEG), no processo de insensibilização para abate humanitário de tilápia do Nilo |
title |
Unconsciousness assessment through electroencephalography (EEG), in the process of stunning for humane slaughter of Nile tilapia |
spellingShingle |
Unconsciousness assessment through electroencephalography (EEG), in the process of stunning for humane slaughter of Nile tilapia Rucinque, Daniel Santiago Abate Anaesthesia Anestesia Animal welfare Behaviour indicators Bem-estar animal Bem-estar de peixes Essential oils Fish welfare Hipotermia Hypothermia Indicadores de comportamento Insensibilização Meat quality Óleos essenciais Qualidade da carne Slaughter Stunning Unconsciousness |
title_short |
Unconsciousness assessment through electroencephalography (EEG), in the process of stunning for humane slaughter of Nile tilapia |
title_full |
Unconsciousness assessment through electroencephalography (EEG), in the process of stunning for humane slaughter of Nile tilapia |
title_fullStr |
Unconsciousness assessment through electroencephalography (EEG), in the process of stunning for humane slaughter of Nile tilapia |
title_full_unstemmed |
Unconsciousness assessment through electroencephalography (EEG), in the process of stunning for humane slaughter of Nile tilapia |
title_sort |
Unconsciousness assessment through electroencephalography (EEG), in the process of stunning for humane slaughter of Nile tilapia |
author |
Rucinque, Daniel Santiago |
author_facet |
Rucinque, Daniel Santiago |
author_role |
author |
dc.contributor.none.fl_str_mv |
Viegas, Elisabete Maria Macedo |
dc.contributor.author.fl_str_mv |
Rucinque, Daniel Santiago |
dc.subject.por.fl_str_mv |
Abate Anaesthesia Anestesia Animal welfare Behaviour indicators Bem-estar animal Bem-estar de peixes Essential oils Fish welfare Hipotermia Hypothermia Indicadores de comportamento Insensibilização Meat quality Óleos essenciais Qualidade da carne Slaughter Stunning Unconsciousness |
topic |
Abate Anaesthesia Anestesia Animal welfare Behaviour indicators Bem-estar animal Bem-estar de peixes Essential oils Fish welfare Hipotermia Hypothermia Indicadores de comportamento Insensibilização Meat quality Óleos essenciais Qualidade da carne Slaughter Stunning Unconsciousness |
description |
Chapter 1 presents a brief introduction to the subject and the aim of these. Chapter 2 assessed Ocimum americanum (OA) or Lippia alba (LA) essential oils as anaesthetics in Nile tilapia and their influence on the aroma. For the anaesthetic evaluation, Nile tilapia were divided into four groups: ethanol (ET), 2-phenoxyethanol (PE), Ocimum americanum (OA) or Lippia alba (LA), all at 500 µL/L. OA or LA essential oils at 500 µL/L were effective in inducing deep anaesthesia with unconsciousness duration of 126 s, allowing time for bleeding. Anaesthesia with Ocimum americanum or Lippia alba essential oils may be indicated as an alternative to hypothermia at pre-slaughter handling of fish in Brazil. Chapter 3 aimed to assess mechanical spiking and electrical stunning as methods to induce unconsciousness in Nile tilapia, and their effects on rigor mortis. Nile tilapia were divided into three groups, two groups for spiking and one group for electrical stunning. Mechanical spiking with lateral access is effective to induce unconsciousness without recovery, delaying the onset of rigor mortis in Nile tilapia, thus its use in an automatic system could be an alternative to traditional slaughter of hypothermia in Brazil. Chapter 4 aimed to assess unconsciousness using electroencephalography EEG in Nile tilapia under different methods at pre-slaughter. Nile tilapia were divided in five different methods at pre-slaughter: anaesthesia with phenoxyethanol at 1 mL/L (PE), anaesthesia with Ocimum americanum (OA) or Lippia alba (LA) essential oils at 500 µL/L, spiking (SP) and hypothermia in ice/water (2:1). The median time (s) to lose consciousness as represented by the suppressed phase was PE (136 ± 61), OA (171 ± 164), LA (171.5 ±77.8) and HP (252 ± 752) (P<0.01). Spectral analysis suggested unconsciousness after 3 minutes of exposure to PE, OA or LA due to the decreases in Ptot, F50 and F95, and also the increase in contribution from the delta frequency and decrease in contribution from the beta frequency. The values obtained in the spectral analysis for fish submitted to HP suggest that this method does not induce unconsciousness. When used in Nile tilapia, phenoxyethanol-like anaesthetic showed both EEG traces and spectral analyses consistent with unconsciousness after 140 s of immersion. Anaesthesia with 500µL/L of the essential oils Ocimum americanum or Lippia alba induced unconsciousness after 180 s of immersion, as determined by EEG. The use of hypothermia at pre-slaughter of Nile tilapia does not induce unconsciousness and should be urgently substituted by methods that meet criteria of humane slaughter. Chapter 5 assessed the influence of different pre-slaughter methods: hypothermia live chilling (HP); anaesthesia with the essential oils Ocimum americanum (OA) or Lippia alba (LA) and mechanical spiking (SP) on the meat quality parameters of Nile tilapia stored under refrigeration for 15 days. The use of mechanical spiking and anaesthesia with the Ocimum americanum (OA) or Lippia alba (LA) essential oils in Nile tilapia did not negatively change the following quality parameters of the meat: pH value, rigor mortis, TVB-N and K-value as compared to the traditional method of hypothermia. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-27 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.teses.usp.br/teses/disponiveis/74/74131/tde-30042021-121501/ |
url |
https://www.teses.usp.br/teses/disponiveis/74/74131/tde-30042021-121501/ |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
|
dc.rights.driver.fl_str_mv |
Liberar o conteúdo para acesso público. info:eu-repo/semantics/openAccess |
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Liberar o conteúdo para acesso público. |
eu_rights_str_mv |
openAccess |
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application/pdf |
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|
dc.publisher.none.fl_str_mv |
Biblioteca Digitais de Teses e Dissertações da USP |
publisher.none.fl_str_mv |
Biblioteca Digitais de Teses e Dissertações da USP |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da USP instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
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USP |
institution |
USP |
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Biblioteca Digital de Teses e Dissertações da USP |
collection |
Biblioteca Digital de Teses e Dissertações da USP |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br |
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1815257499141406720 |