First investigation of genetic control of meiosis in sugarcane
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Tipo de documento: | Tese |
Idioma: | eng |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da USP |
Texto Completo: | https://www.teses.usp.br/teses/disponiveis/11/11137/tde-05012024-155836/ |
Resumo: | Sugarcane (Saccharum spp.) has one of the most complex crop genomes. Modern varieties are highly polyploids and aneuploids, resulting from interspecific hybridization between Saccharum officinarum and S. spontaneum. Research on the meiotic control in polyploid species is limited, with the exception of wheat Ph1 locus, carrying the meiotic gene ZIP4 (TaZIP4-B2) that promotes pairing and synapsis between homologues, whilst suppressing CO between homoeologous chromosomes. In sugarcane, despite its interspecific origin, bivalent association is favored, and multivalents, if they occur, are resolved at the end of meiosis I. Our aim here was to investigate the supposedly control of synapsis in sugarcane. We have investigated the ZIP4 gene and performed the immunolocalization of meiotic proteins, namely the synaptonemal complex proteins ZYP1 and ASY1. The sugarcane ZIP4 gene is located on chromosome 3 and it is expressed more abundantly in flowers, showing a similar profile to that of TaZIP4-B2. The expression level of ZIP4 is higher in S. spontaneum, an evolving autopolyploid, and less expressed in S. officinarum, a legitimate octaploid species. The ZIP4 protein contains a TPR domain, essential to its scaffolding function, and the 3D structure prediction is highly similar to that of TaZIP4-B2. The immunolocalization of the ASY1 and ZYP1 proteins revealed that S. officinarum completes synapsis. However, in S. spontaneum and SP80-3280, a modern variety, no nuclei with complete synapsis were observed. Our results will have implications for sugarcane genetic mapping and genomics, whose understanding of inheritance and genome organization remain somewhat obscure. |
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First investigation of genetic control of meiosis in sugarcanePrimeira investigação do controle genético da meiose em cana-de-açúcarSaccharumSaccharumZIP4ZIP4MeioseMeiosisSinapseSynapsisSugarcane (Saccharum spp.) has one of the most complex crop genomes. Modern varieties are highly polyploids and aneuploids, resulting from interspecific hybridization between Saccharum officinarum and S. spontaneum. Research on the meiotic control in polyploid species is limited, with the exception of wheat Ph1 locus, carrying the meiotic gene ZIP4 (TaZIP4-B2) that promotes pairing and synapsis between homologues, whilst suppressing CO between homoeologous chromosomes. In sugarcane, despite its interspecific origin, bivalent association is favored, and multivalents, if they occur, are resolved at the end of meiosis I. Our aim here was to investigate the supposedly control of synapsis in sugarcane. We have investigated the ZIP4 gene and performed the immunolocalization of meiotic proteins, namely the synaptonemal complex proteins ZYP1 and ASY1. The sugarcane ZIP4 gene is located on chromosome 3 and it is expressed more abundantly in flowers, showing a similar profile to that of TaZIP4-B2. The expression level of ZIP4 is higher in S. spontaneum, an evolving autopolyploid, and less expressed in S. officinarum, a legitimate octaploid species. The ZIP4 protein contains a TPR domain, essential to its scaffolding function, and the 3D structure prediction is highly similar to that of TaZIP4-B2. The immunolocalization of the ASY1 and ZYP1 proteins revealed that S. officinarum completes synapsis. However, in S. spontaneum and SP80-3280, a modern variety, no nuclei with complete synapsis were observed. Our results will have implications for sugarcane genetic mapping and genomics, whose understanding of inheritance and genome organization remain somewhat obscure.A cana-de-açúcar (Saccharum spp.) é a espécie cultivada com um dos genomas mais complexos. As variedades modernas são altamente poliplóides e aneuploides, resultantes da hibridização interespecífica entre Saccharum officinarum e S. spontaneum. A pesquisa sobre o controle meiótico em espécies poliplóides é limitada, com exceção do locus Ph1 do trigo, que carrega o gene meiótico ZIP4 (TaZIP4-B2) que promove o pareamento e a sinapse entre homólogos, enquanto suprime o CO entre os cromossomos homoeólogos. Na cana-de-açúcar, apesar de sua origem interespecífica, a associação bivalente é favorecida, e os multivalentes, caso ocorram, são resolvidos ao final da meiose I. Nosso objetivo aqui foi investigar o suposto controle da sinapse na cana-de-açúcar. Investigamos o gene ZIP4 e realizamos a imunolocalização de proteínas meióticas, especificamente as proteínas do complexo sinaptonêmico ZYP1 e ASY1. O gene ZIP4 da cana-de-açúcar está localizado no cromossomo 3 e é expresso mais abundantemente nas flores, apresentando perfil semelhante ao do TaZIP4- B2. O nível de expressão de ZIP4 é maior em S. spontaneum, um autopoliplóide em evolução, e menos expresso em S. officinarum, uma espécie octaplóide legítima. A proteína ZIP4 contém um domínio TPR, essencial para a sua função de scaffold, e a predição da estrutura 3D é altamente semelhante à do TaZIP4-B2. A imunolocalização das proteínas ASY1 e ZYP1 revelou que S. officinarum completa a sinapse. Porém, em S. spontaneum e SP80-3280, uma variedade moderna, não foram observados núcleos com sinapse completa. Nossos resultados terão implicações para o mapeamento genético e a genômica da cana-de-açúcar, cuja compreensão da herança e da organização do genoma permanece um tanto obscura.Biblioteca Digitais de Teses e Dissertações da USPVieira, Maria Lucia CarneiroSoares, Nina Reis2023-10-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttps://www.teses.usp.br/teses/disponiveis/11/11137/tde-05012024-155836/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2024-01-08T17:50:02Zoai:teses.usp.br:tde-05012024-155836Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212024-01-08T17:50:02Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
First investigation of genetic control of meiosis in sugarcane Primeira investigação do controle genético da meiose em cana-de-açúcar |
title |
First investigation of genetic control of meiosis in sugarcane |
spellingShingle |
First investigation of genetic control of meiosis in sugarcane Soares, Nina Reis Saccharum Saccharum ZIP4 ZIP4 Meiose Meiosis Sinapse Synapsis |
title_short |
First investigation of genetic control of meiosis in sugarcane |
title_full |
First investigation of genetic control of meiosis in sugarcane |
title_fullStr |
First investigation of genetic control of meiosis in sugarcane |
title_full_unstemmed |
First investigation of genetic control of meiosis in sugarcane |
title_sort |
First investigation of genetic control of meiosis in sugarcane |
author |
Soares, Nina Reis |
author_facet |
Soares, Nina Reis |
author_role |
author |
dc.contributor.none.fl_str_mv |
Vieira, Maria Lucia Carneiro |
dc.contributor.author.fl_str_mv |
Soares, Nina Reis |
dc.subject.por.fl_str_mv |
Saccharum Saccharum ZIP4 ZIP4 Meiose Meiosis Sinapse Synapsis |
topic |
Saccharum Saccharum ZIP4 ZIP4 Meiose Meiosis Sinapse Synapsis |
description |
Sugarcane (Saccharum spp.) has one of the most complex crop genomes. Modern varieties are highly polyploids and aneuploids, resulting from interspecific hybridization between Saccharum officinarum and S. spontaneum. Research on the meiotic control in polyploid species is limited, with the exception of wheat Ph1 locus, carrying the meiotic gene ZIP4 (TaZIP4-B2) that promotes pairing and synapsis between homologues, whilst suppressing CO between homoeologous chromosomes. In sugarcane, despite its interspecific origin, bivalent association is favored, and multivalents, if they occur, are resolved at the end of meiosis I. Our aim here was to investigate the supposedly control of synapsis in sugarcane. We have investigated the ZIP4 gene and performed the immunolocalization of meiotic proteins, namely the synaptonemal complex proteins ZYP1 and ASY1. The sugarcane ZIP4 gene is located on chromosome 3 and it is expressed more abundantly in flowers, showing a similar profile to that of TaZIP4-B2. The expression level of ZIP4 is higher in S. spontaneum, an evolving autopolyploid, and less expressed in S. officinarum, a legitimate octaploid species. The ZIP4 protein contains a TPR domain, essential to its scaffolding function, and the 3D structure prediction is highly similar to that of TaZIP4-B2. The immunolocalization of the ASY1 and ZYP1 proteins revealed that S. officinarum completes synapsis. However, in S. spontaneum and SP80-3280, a modern variety, no nuclei with complete synapsis were observed. Our results will have implications for sugarcane genetic mapping and genomics, whose understanding of inheritance and genome organization remain somewhat obscure. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-10-27 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.teses.usp.br/teses/disponiveis/11/11137/tde-05012024-155836/ |
url |
https://www.teses.usp.br/teses/disponiveis/11/11137/tde-05012024-155836/ |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
|
dc.rights.driver.fl_str_mv |
Liberar o conteúdo para acesso público. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Liberar o conteúdo para acesso público. |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
|
dc.publisher.none.fl_str_mv |
Biblioteca Digitais de Teses e Dissertações da USP |
publisher.none.fl_str_mv |
Biblioteca Digitais de Teses e Dissertações da USP |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da USP instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Biblioteca Digital de Teses e Dissertações da USP |
collection |
Biblioteca Digital de Teses e Dissertações da USP |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br |
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1809090396341403648 |