Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compounds

Detalhes bibliográficos
Autor(a) principal: Ikeda, Natália Yumi
Data de Publicação: 2020
Tipo de documento: Tese
Idioma: eng
Título da fonte: Biblioteca Digital de Teses e Dissertações da USP
Texto Completo: http://www.teses.usp.br/teses/disponiveis/11/11141/tde-04062020-084904/
Resumo: Ethanolic extract of propolis (EEP) is one of the most effective ways to extract the active compounds of propolis resin. However, EEP\'s processing chain generates a great amount of residues. This residue is composed of several substances such as wax, resins and gums, and also could still contains some bioactive compounds. However, it cannot be reused for human consumption, but it could be applied in animal feed. This study investigated two different forms to use the residues from ethanolic extraction of organic propolis, the extraction of essential oils (EOs) and phenolic acids fractions. EOs were extracted through hydrodistillation and phenolic acids fractions were obtained through solvent extractions and alkaline/acid hydrolysis methods. Both compounds were evaluated for total phenolic content, antioxidant capacity in terms of ABTS, DPPH and FRAP, and antibacterial activity against a pathogenic (Escherichia coli) and a beneficial (Lactobacillus plantarum) bacterium model. All parameters were compared with ethanolic extract of propolis (EEP). This study aimed to investigate propolis residues as a source of biological compounds that could be added in animal feed as an alternative to synthetic antimicrobials.
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spelling Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compoundsAvaliação dos resíduos obtidos da extração etanólica da própolis orgânica como fonte de compostos biológicosE. coliE. coliLactobacillusLactobacillusÁcidos fenólicosEssential oilÓleo essencialPhenolic acidsPropolis residueResíduoEthanolic extract of propolis (EEP) is one of the most effective ways to extract the active compounds of propolis resin. However, EEP\'s processing chain generates a great amount of residues. This residue is composed of several substances such as wax, resins and gums, and also could still contains some bioactive compounds. However, it cannot be reused for human consumption, but it could be applied in animal feed. This study investigated two different forms to use the residues from ethanolic extraction of organic propolis, the extraction of essential oils (EOs) and phenolic acids fractions. EOs were extracted through hydrodistillation and phenolic acids fractions were obtained through solvent extractions and alkaline/acid hydrolysis methods. Both compounds were evaluated for total phenolic content, antioxidant capacity in terms of ABTS, DPPH and FRAP, and antibacterial activity against a pathogenic (Escherichia coli) and a beneficial (Lactobacillus plantarum) bacterium model. All parameters were compared with ethanolic extract of propolis (EEP). This study aimed to investigate propolis residues as a source of biological compounds that could be added in animal feed as an alternative to synthetic antimicrobials.O extrato etanólico de própolis (EEP) é a forma mais eficiente de extrair os compostos biológicos da própolis. Entretanto, a cadeia produtiva do EEP produz grande quantidade de resíduos. Esse resíduo é composto por diversas substâncias como cera, resinas e gomas, e ainda pode conter alguns compostos biológicos. Entretanto, esse resíduo não pode ser reutilizado para o consumo humano, mas poderia ser aproveitado na alimentação animal. Este estudo investigou duas formas diferentes de utilizar os resíduos da extração etanólica da própolis orgânica, a extração de óleos essenciais (OEs) e de frações de ácidos fenólicos. Os OEs foram extraídos por hidrodestilação e as frações de ácidos fenólicos foram obtidas por extrações com solventes e hidrólise alcalina/ácida. Ambos os compostos foram avaliados em relação a concentração de fenólicos totais, capacidade antioxidante por ABTS, DPPH e FRAP e atividade antibacteriana contra um modelo de bactéria patogênica (Escherichia coli) e uma benéfica (Lactobacillus plantarum). Todos os parâmetros foram comparados com o extrato etanólico de própolis (EEP). Este estudo teve como objetivo investigar os resíduos da própolis como fonte de compostos biológicos que poderiam ser adicionados na alimentação animal como uma alternativa aos antimicrobianos sintéticos.Biblioteca Digitais de Teses e Dissertações da USPAlencar, Severino Matias deIkeda, Natália Yumi2020-04-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://www.teses.usp.br/teses/disponiveis/11/11141/tde-04062020-084904/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2020-06-05T20:57:02Zoai:teses.usp.br:tde-04062020-084904Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212020-06-05T20:57:02Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compounds
Avaliação dos resíduos obtidos da extração etanólica da própolis orgânica como fonte de compostos biológicos
title Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compounds
spellingShingle Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compounds
Ikeda, Natália Yumi
E. coli
E. coli
Lactobacillus
Lactobacillus
Ácidos fenólicos
Essential oil
Óleo essencial
Phenolic acids
Propolis residue
Resíduo
title_short Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compounds
title_full Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compounds
title_fullStr Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compounds
title_full_unstemmed Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compounds
title_sort Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compounds
author Ikeda, Natália Yumi
author_facet Ikeda, Natália Yumi
author_role author
dc.contributor.none.fl_str_mv Alencar, Severino Matias de
dc.contributor.author.fl_str_mv Ikeda, Natália Yumi
dc.subject.por.fl_str_mv E. coli
E. coli
Lactobacillus
Lactobacillus
Ácidos fenólicos
Essential oil
Óleo essencial
Phenolic acids
Propolis residue
Resíduo
topic E. coli
E. coli
Lactobacillus
Lactobacillus
Ácidos fenólicos
Essential oil
Óleo essencial
Phenolic acids
Propolis residue
Resíduo
description Ethanolic extract of propolis (EEP) is one of the most effective ways to extract the active compounds of propolis resin. However, EEP\'s processing chain generates a great amount of residues. This residue is composed of several substances such as wax, resins and gums, and also could still contains some bioactive compounds. However, it cannot be reused for human consumption, but it could be applied in animal feed. This study investigated two different forms to use the residues from ethanolic extraction of organic propolis, the extraction of essential oils (EOs) and phenolic acids fractions. EOs were extracted through hydrodistillation and phenolic acids fractions were obtained through solvent extractions and alkaline/acid hydrolysis methods. Both compounds were evaluated for total phenolic content, antioxidant capacity in terms of ABTS, DPPH and FRAP, and antibacterial activity against a pathogenic (Escherichia coli) and a beneficial (Lactobacillus plantarum) bacterium model. All parameters were compared with ethanolic extract of propolis (EEP). This study aimed to investigate propolis residues as a source of biological compounds that could be added in animal feed as an alternative to synthetic antimicrobials.
publishDate 2020
dc.date.none.fl_str_mv 2020-04-02
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.teses.usp.br/teses/disponiveis/11/11141/tde-04062020-084904/
url http://www.teses.usp.br/teses/disponiveis/11/11141/tde-04062020-084904/
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv
dc.rights.driver.fl_str_mv Liberar o conteúdo para acesso público.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Liberar o conteúdo para acesso público.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv
dc.publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
dc.source.none.fl_str_mv
reponame:Biblioteca Digital de Teses e Dissertações da USP
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Biblioteca Digital de Teses e Dissertações da USP
collection Biblioteca Digital de Teses e Dissertações da USP
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)
repository.mail.fl_str_mv virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br
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