Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compounds
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Tipo de documento: | Tese |
Idioma: | eng |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da USP |
Texto Completo: | http://www.teses.usp.br/teses/disponiveis/11/11141/tde-04062020-084904/ |
Resumo: | Ethanolic extract of propolis (EEP) is one of the most effective ways to extract the active compounds of propolis resin. However, EEP\'s processing chain generates a great amount of residues. This residue is composed of several substances such as wax, resins and gums, and also could still contains some bioactive compounds. However, it cannot be reused for human consumption, but it could be applied in animal feed. This study investigated two different forms to use the residues from ethanolic extraction of organic propolis, the extraction of essential oils (EOs) and phenolic acids fractions. EOs were extracted through hydrodistillation and phenolic acids fractions were obtained through solvent extractions and alkaline/acid hydrolysis methods. Both compounds were evaluated for total phenolic content, antioxidant capacity in terms of ABTS, DPPH and FRAP, and antibacterial activity against a pathogenic (Escherichia coli) and a beneficial (Lactobacillus plantarum) bacterium model. All parameters were compared with ethanolic extract of propolis (EEP). This study aimed to investigate propolis residues as a source of biological compounds that could be added in animal feed as an alternative to synthetic antimicrobials. |
id |
USP_11844f3c51cd8c9b296cb60914c4e4e0 |
---|---|
oai_identifier_str |
oai:teses.usp.br:tde-04062020-084904 |
network_acronym_str |
USP |
network_name_str |
Biblioteca Digital de Teses e Dissertações da USP |
repository_id_str |
2721 |
spelling |
Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compoundsAvaliação dos resíduos obtidos da extração etanólica da própolis orgânica como fonte de compostos biológicosE. coliE. coliLactobacillusLactobacillusÁcidos fenólicosEssential oilÓleo essencialPhenolic acidsPropolis residueResíduoEthanolic extract of propolis (EEP) is one of the most effective ways to extract the active compounds of propolis resin. However, EEP\'s processing chain generates a great amount of residues. This residue is composed of several substances such as wax, resins and gums, and also could still contains some bioactive compounds. However, it cannot be reused for human consumption, but it could be applied in animal feed. This study investigated two different forms to use the residues from ethanolic extraction of organic propolis, the extraction of essential oils (EOs) and phenolic acids fractions. EOs were extracted through hydrodistillation and phenolic acids fractions were obtained through solvent extractions and alkaline/acid hydrolysis methods. Both compounds were evaluated for total phenolic content, antioxidant capacity in terms of ABTS, DPPH and FRAP, and antibacterial activity against a pathogenic (Escherichia coli) and a beneficial (Lactobacillus plantarum) bacterium model. All parameters were compared with ethanolic extract of propolis (EEP). This study aimed to investigate propolis residues as a source of biological compounds that could be added in animal feed as an alternative to synthetic antimicrobials.O extrato etanólico de própolis (EEP) é a forma mais eficiente de extrair os compostos biológicos da própolis. Entretanto, a cadeia produtiva do EEP produz grande quantidade de resíduos. Esse resíduo é composto por diversas substâncias como cera, resinas e gomas, e ainda pode conter alguns compostos biológicos. Entretanto, esse resíduo não pode ser reutilizado para o consumo humano, mas poderia ser aproveitado na alimentação animal. Este estudo investigou duas formas diferentes de utilizar os resíduos da extração etanólica da própolis orgânica, a extração de óleos essenciais (OEs) e de frações de ácidos fenólicos. Os OEs foram extraídos por hidrodestilação e as frações de ácidos fenólicos foram obtidas por extrações com solventes e hidrólise alcalina/ácida. Ambos os compostos foram avaliados em relação a concentração de fenólicos totais, capacidade antioxidante por ABTS, DPPH e FRAP e atividade antibacteriana contra um modelo de bactéria patogênica (Escherichia coli) e uma benéfica (Lactobacillus plantarum). Todos os parâmetros foram comparados com o extrato etanólico de própolis (EEP). Este estudo teve como objetivo investigar os resíduos da própolis como fonte de compostos biológicos que poderiam ser adicionados na alimentação animal como uma alternativa aos antimicrobianos sintéticos.Biblioteca Digitais de Teses e Dissertações da USPAlencar, Severino Matias deIkeda, Natália Yumi2020-04-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://www.teses.usp.br/teses/disponiveis/11/11141/tde-04062020-084904/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2020-06-05T20:57:02Zoai:teses.usp.br:tde-04062020-084904Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212020-06-05T20:57:02Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compounds Avaliação dos resíduos obtidos da extração etanólica da própolis orgânica como fonte de compostos biológicos |
title |
Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compounds |
spellingShingle |
Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compounds Ikeda, Natália Yumi E. coli E. coli Lactobacillus Lactobacillus Ácidos fenólicos Essential oil Óleo essencial Phenolic acids Propolis residue Resíduo |
title_short |
Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compounds |
title_full |
Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compounds |
title_fullStr |
Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compounds |
title_full_unstemmed |
Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compounds |
title_sort |
Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compounds |
author |
Ikeda, Natália Yumi |
author_facet |
Ikeda, Natália Yumi |
author_role |
author |
dc.contributor.none.fl_str_mv |
Alencar, Severino Matias de |
dc.contributor.author.fl_str_mv |
Ikeda, Natália Yumi |
dc.subject.por.fl_str_mv |
E. coli E. coli Lactobacillus Lactobacillus Ácidos fenólicos Essential oil Óleo essencial Phenolic acids Propolis residue Resíduo |
topic |
E. coli E. coli Lactobacillus Lactobacillus Ácidos fenólicos Essential oil Óleo essencial Phenolic acids Propolis residue Resíduo |
description |
Ethanolic extract of propolis (EEP) is one of the most effective ways to extract the active compounds of propolis resin. However, EEP\'s processing chain generates a great amount of residues. This residue is composed of several substances such as wax, resins and gums, and also could still contains some bioactive compounds. However, it cannot be reused for human consumption, but it could be applied in animal feed. This study investigated two different forms to use the residues from ethanolic extraction of organic propolis, the extraction of essential oils (EOs) and phenolic acids fractions. EOs were extracted through hydrodistillation and phenolic acids fractions were obtained through solvent extractions and alkaline/acid hydrolysis methods. Both compounds were evaluated for total phenolic content, antioxidant capacity in terms of ABTS, DPPH and FRAP, and antibacterial activity against a pathogenic (Escherichia coli) and a beneficial (Lactobacillus plantarum) bacterium model. All parameters were compared with ethanolic extract of propolis (EEP). This study aimed to investigate propolis residues as a source of biological compounds that could be added in animal feed as an alternative to synthetic antimicrobials. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-04-02 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.teses.usp.br/teses/disponiveis/11/11141/tde-04062020-084904/ |
url |
http://www.teses.usp.br/teses/disponiveis/11/11141/tde-04062020-084904/ |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
|
dc.rights.driver.fl_str_mv |
Liberar o conteúdo para acesso público. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Liberar o conteúdo para acesso público. |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
|
dc.publisher.none.fl_str_mv |
Biblioteca Digitais de Teses e Dissertações da USP |
publisher.none.fl_str_mv |
Biblioteca Digitais de Teses e Dissertações da USP |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da USP instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Biblioteca Digital de Teses e Dissertações da USP |
collection |
Biblioteca Digital de Teses e Dissertações da USP |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br |
_version_ |
1815256661321842688 |