Lignocellulosic biomass valorization: a study of plant biomass structure, composition, and enzymatic digestibility after being submitted to different pretreatment steps

Detalhes bibliográficos
Autor(a) principal: Kane, Aissata Ousmane
Data de Publicação: 2022
Tipo de documento: Tese
Idioma: eng
Título da fonte: Biblioteca Digital de Teses e Dissertações da USP
Texto Completo: https://www.teses.usp.br/teses/disponiveis/76/76133/tde-14022023-103652/
Resumo: Bioenergy has been proposed as one way to significantly meet global energy demand and prevent environmental problems using renewable energy resources. The valorization of lignocellulosic biomass as a renewable feedstock for producing bioethanol and biomaterial has attracted much attention. However, efficient lignocellulosic biomass conversions require the development of strategies and technologies safe and inexpensive. Sugarcane is an agricultural crop produced in several tropical countries, notably Brazil. This work has investigated sugarcane wastes as potential raw materials to produce second-generation biofuels and nanomaterials. Different configurations of two-stage pretreatments were applied to sugarcane wastes (SCC, SCB, SCL) to produce fermentable sugars after enzymatic hydrolysis as well as nanocellulose via enzymatic hydrolysis and mechanical disintegration of cellulose-enriched material. Enzymatic hydrolysis yields of raw and pretreated sugarcane wastes were determined and correlated with structural, morphological, and composition changes after pretreatments. Overall, after combined pretreatments, enzymatic convertibility was highly efficient for all studies, reaching over 97% for SCC after acid-alkaline pretreatment (NaOH2% / H2SO42%), 91.2% for SCL acid-alkaline (H2SO41% / NaOH1%), 99.57 % for sugarcane bagasse after liquid hot water - alkaline sulfonation (H2O / Na2SO3 +NaOH). This efficiency was correlated to the removal of amorphous parts of lignocellulosic biomass, lignin, and/or hemicellulose, which also justify high crystallinity indices and crystallite sizes of pretreated biomass. An efficient carbohydrate extraction from sugarcane bagasse occurred during alkaline pretreatment using NaOH + H2O2, with a maximum delignification. Thermally and colloidally stable cellulose nanofibers were obtained. The enzymatic hydrolysis stage facilitated the defibrillation of blanched SCB, and the sulfonation introduced sulfonic groups in the CNF. The introduction of surface charges to CNF promotes their functionalization and widens their fields of application.
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spelling Lignocellulosic biomass valorization: a study of plant biomass structure, composition, and enzymatic digestibility after being submitted to different pretreatment stepsValorização da biomassa lignocelulósica: estudo da estrutura, composição e digestibilidade enzimática da biomassa vegetal após ser submetida a diferentes etapas de pré-tratamentoBiomassa lignocelulósicaCellulose nanofibers (CNF)Hidrólise enzimáticaLignocellulosic biomassNanofibras de celulosePré-tratamentoPretreatment. Enzymatic hydrolysisBioenergy has been proposed as one way to significantly meet global energy demand and prevent environmental problems using renewable energy resources. The valorization of lignocellulosic biomass as a renewable feedstock for producing bioethanol and biomaterial has attracted much attention. However, efficient lignocellulosic biomass conversions require the development of strategies and technologies safe and inexpensive. Sugarcane is an agricultural crop produced in several tropical countries, notably Brazil. This work has investigated sugarcane wastes as potential raw materials to produce second-generation biofuels and nanomaterials. Different configurations of two-stage pretreatments were applied to sugarcane wastes (SCC, SCB, SCL) to produce fermentable sugars after enzymatic hydrolysis as well as nanocellulose via enzymatic hydrolysis and mechanical disintegration of cellulose-enriched material. Enzymatic hydrolysis yields of raw and pretreated sugarcane wastes were determined and correlated with structural, morphological, and composition changes after pretreatments. Overall, after combined pretreatments, enzymatic convertibility was highly efficient for all studies, reaching over 97% for SCC after acid-alkaline pretreatment (NaOH2% / H2SO42%), 91.2% for SCL acid-alkaline (H2SO41% / NaOH1%), 99.57 % for sugarcane bagasse after liquid hot water - alkaline sulfonation (H2O / Na2SO3 +NaOH). This efficiency was correlated to the removal of amorphous parts of lignocellulosic biomass, lignin, and/or hemicellulose, which also justify high crystallinity indices and crystallite sizes of pretreated biomass. An efficient carbohydrate extraction from sugarcane bagasse occurred during alkaline pretreatment using NaOH + H2O2, with a maximum delignification. Thermally and colloidally stable cellulose nanofibers were obtained. The enzymatic hydrolysis stage facilitated the defibrillation of blanched SCB, and the sulfonation introduced sulfonic groups in the CNF. The introduction of surface charges to CNF promotes their functionalization and widens their fields of application.A bioenergia tem sido proposta como uma forma de atender significativamente a demanda energética global e prevenir problemas ambientais, utilizando recursos energéticos renováveis. A valorização da biomassa lignocelulósica como matéria-prima renovável para a produção de bioetanol e biomaterial tem despertado muita atenção. No entanto, a transformação eficiente da biomassa lignocelulósica em açúcares fermentescíveis requer o desenvolvimento de estratégias e tecnologias para rupturas da biomassa lignocelulósica. A cana-de-açúcar é uma importante cultura agrícola produzida em vários países tropicais, notadamente no Brasil. Este trabalho investigou resíduos de cana-de-açúcar como potenciais matérias-primas para a produção de biocombustíveis e nano materiais de segunda geração. Diferentes configurações de pré-tratamentos de duas etapas foram aplicadas aos resíduos de cana-de-açúcar (SC, SCB, SCL) para produzir açúcares fermentáveis após hidrólise enzimática, bem como nanocelulose via hidrólise enzimática e desintegração mecânica do material rico em celulose. Os rendimentos de hidrólise enzimática de resíduos de cana-de-açúcar não tratados e pré-tratados foram determinados e correlacionados com alterações estruturais, morfológicas e de composição após os pré-tratamentos. No geral, após pré-tratamentos combinados, a convertibilidade enzimática foi altamente eficiente para todos os estudos (capítulo 4-6, atingindo mais de 97% para SCC após pré-tratamento ácido-alcalino (NaOH2%/H2SO42%), 91,2% para SCL ácido-alcalino (H2SO41% / NaOH1%), 99,57 % para bagaço de cana após LHW- sulfonação alcalina (H2O/Na2SO3+NaOH). Essa eficiência foi correlacionada com a remoção de partes amorfas de biomassa lignocelulósica, lignina e/ou hemicelulose, que também justificam a alta cristalinidade índices e tamanhos de cristalitos da biomassa pré-tratada. Um eficiente fracionamento do bagaço de cana-de-açúcar ocorreu durante o pré-tratamento alcalino com NaOH+H2O2, com máxima deslignificação. A etapa de hidrólise enzimática facilitou a desfibrilação da biomassa branqueada, e a sulfonação da biomassa branqueada melhorou sua digestibilidade enzimática. A introdução de superficiais no CNF promove suas funcionalizações e amplia seus campos de aplicação.Biblioteca Digitais de Teses e Dissertações da USPPolikarpov, IgorKane, Aissata Ousmane2022-12-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttps://www.teses.usp.br/teses/disponiveis/76/76133/tde-14022023-103652/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2024-08-22T23:46:03Zoai:teses.usp.br:tde-14022023-103652Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212024-08-22T23:46:03Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Lignocellulosic biomass valorization: a study of plant biomass structure, composition, and enzymatic digestibility after being submitted to different pretreatment steps
Valorização da biomassa lignocelulósica: estudo da estrutura, composição e digestibilidade enzimática da biomassa vegetal após ser submetida a diferentes etapas de pré-tratamento
title Lignocellulosic biomass valorization: a study of plant biomass structure, composition, and enzymatic digestibility after being submitted to different pretreatment steps
spellingShingle Lignocellulosic biomass valorization: a study of plant biomass structure, composition, and enzymatic digestibility after being submitted to different pretreatment steps
Kane, Aissata Ousmane
Biomassa lignocelulósica
Cellulose nanofibers (CNF)
Hidrólise enzimática
Lignocellulosic biomass
Nanofibras de celulose
Pré-tratamento
Pretreatment. Enzymatic hydrolysis
title_short Lignocellulosic biomass valorization: a study of plant biomass structure, composition, and enzymatic digestibility after being submitted to different pretreatment steps
title_full Lignocellulosic biomass valorization: a study of plant biomass structure, composition, and enzymatic digestibility after being submitted to different pretreatment steps
title_fullStr Lignocellulosic biomass valorization: a study of plant biomass structure, composition, and enzymatic digestibility after being submitted to different pretreatment steps
title_full_unstemmed Lignocellulosic biomass valorization: a study of plant biomass structure, composition, and enzymatic digestibility after being submitted to different pretreatment steps
title_sort Lignocellulosic biomass valorization: a study of plant biomass structure, composition, and enzymatic digestibility after being submitted to different pretreatment steps
author Kane, Aissata Ousmane
author_facet Kane, Aissata Ousmane
author_role author
dc.contributor.none.fl_str_mv Polikarpov, Igor
dc.contributor.author.fl_str_mv Kane, Aissata Ousmane
dc.subject.por.fl_str_mv Biomassa lignocelulósica
Cellulose nanofibers (CNF)
Hidrólise enzimática
Lignocellulosic biomass
Nanofibras de celulose
Pré-tratamento
Pretreatment. Enzymatic hydrolysis
topic Biomassa lignocelulósica
Cellulose nanofibers (CNF)
Hidrólise enzimática
Lignocellulosic biomass
Nanofibras de celulose
Pré-tratamento
Pretreatment. Enzymatic hydrolysis
description Bioenergy has been proposed as one way to significantly meet global energy demand and prevent environmental problems using renewable energy resources. The valorization of lignocellulosic biomass as a renewable feedstock for producing bioethanol and biomaterial has attracted much attention. However, efficient lignocellulosic biomass conversions require the development of strategies and technologies safe and inexpensive. Sugarcane is an agricultural crop produced in several tropical countries, notably Brazil. This work has investigated sugarcane wastes as potential raw materials to produce second-generation biofuels and nanomaterials. Different configurations of two-stage pretreatments were applied to sugarcane wastes (SCC, SCB, SCL) to produce fermentable sugars after enzymatic hydrolysis as well as nanocellulose via enzymatic hydrolysis and mechanical disintegration of cellulose-enriched material. Enzymatic hydrolysis yields of raw and pretreated sugarcane wastes were determined and correlated with structural, morphological, and composition changes after pretreatments. Overall, after combined pretreatments, enzymatic convertibility was highly efficient for all studies, reaching over 97% for SCC after acid-alkaline pretreatment (NaOH2% / H2SO42%), 91.2% for SCL acid-alkaline (H2SO41% / NaOH1%), 99.57 % for sugarcane bagasse after liquid hot water - alkaline sulfonation (H2O / Na2SO3 +NaOH). This efficiency was correlated to the removal of amorphous parts of lignocellulosic biomass, lignin, and/or hemicellulose, which also justify high crystallinity indices and crystallite sizes of pretreated biomass. An efficient carbohydrate extraction from sugarcane bagasse occurred during alkaline pretreatment using NaOH + H2O2, with a maximum delignification. Thermally and colloidally stable cellulose nanofibers were obtained. The enzymatic hydrolysis stage facilitated the defibrillation of blanched SCB, and the sulfonation introduced sulfonic groups in the CNF. The introduction of surface charges to CNF promotes their functionalization and widens their fields of application.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-02
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
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url https://www.teses.usp.br/teses/disponiveis/76/76133/tde-14022023-103652/
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv
dc.rights.driver.fl_str_mv Liberar o conteúdo para acesso público.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Liberar o conteúdo para acesso público.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
dc.source.none.fl_str_mv
reponame:Biblioteca Digital de Teses e Dissertações da USP
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Biblioteca Digital de Teses e Dissertações da USP
collection Biblioteca Digital de Teses e Dissertações da USP
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)
repository.mail.fl_str_mv virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br
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