Drosophila melanogaster primary cell cultures as tools for neurobiology: focusing on mitochondria and Alzheimer\'s disease
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Tipo de documento: | Tese |
Idioma: | eng |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da USP |
Texto Completo: | https://www.teses.usp.br/teses/disponiveis/17/17131/tde-10102023-112050/ |
Resumo: | Brains are the most complex known objects of universe. Such a complexity emerges from structure and connectivity between neurons, their main building blocks. These are high polarized cells, shaped to capture, to process and to convey electrical stimuli via synapses, orchestrating uncountable discrete signals that give rise to sophisticated phenomena, as consciousness. Neurodegenerative diseases are a set of devastating brain affections that course with neuronal senescence and death. Alzheimer\'s disease (AD), the leading cause of senile dementia worldwide, is a neurodegenerative disease characterized by progessive loss of cogniton and higher functions, due to diffuse central neurotoxicity, putatively attributed to senile plaques, composed of toxic amyloid peptides. Here I introduce a new, innovative, in vitro model for AD, based on third instar Drosophila melanogaster larval brains. The model proved adequate for live cell imaging, using fluorescent probes that allowed membrane, nuclear, mitochondrial and lysosomal stainings. Moreover, results obtained were robust and reproducible. Morphometric data indicate that amyloid missexpression in neurons lead to distinct structural phenotypes on the observed cells, leveraging potential uses of the model. |
id |
USP_1c2b8026024192b2279de5f4ad437247 |
---|---|
oai_identifier_str |
oai:teses.usp.br:tde-10102023-112050 |
network_acronym_str |
USP |
network_name_str |
Biblioteca Digital de Teses e Dissertações da USP |
repository_id_str |
2721 |
spelling |
Drosophila melanogaster primary cell cultures as tools for neurobiology: focusing on mitochondria and Alzheimer\'s diseaseCultivos celulares primários de Drosophila melanogaster como ferramentas em neurobiologia: foco em mitocôndrias e doença de AlzheimerAlzheimer's diseaseCulturas neuronaisDoença de AlzheimerDrosophila melanogasterDrosophila melanogasterMitochondriaMitocôndriaMitofagiaMitophagyNeuronal culturesBrains are the most complex known objects of universe. Such a complexity emerges from structure and connectivity between neurons, their main building blocks. These are high polarized cells, shaped to capture, to process and to convey electrical stimuli via synapses, orchestrating uncountable discrete signals that give rise to sophisticated phenomena, as consciousness. Neurodegenerative diseases are a set of devastating brain affections that course with neuronal senescence and death. Alzheimer\'s disease (AD), the leading cause of senile dementia worldwide, is a neurodegenerative disease characterized by progessive loss of cogniton and higher functions, due to diffuse central neurotoxicity, putatively attributed to senile plaques, composed of toxic amyloid peptides. Here I introduce a new, innovative, in vitro model for AD, based on third instar Drosophila melanogaster larval brains. The model proved adequate for live cell imaging, using fluorescent probes that allowed membrane, nuclear, mitochondrial and lysosomal stainings. Moreover, results obtained were robust and reproducible. Morphometric data indicate that amyloid missexpression in neurons lead to distinct structural phenotypes on the observed cells, leveraging potential uses of the model.Cérebros são os objetos mais complexos do universo conhecido. Tal complexidade emerge da estrutura e conectividade entre neurônios, as suas principais células. Estes são altamente polarizados, moldados para captar, processar e transmitir estímulos elétricos através das sinapses, orquestrando inúmeros sinais discretos que dão origem a fenômenos sofisticados, como a consciência. As doenças neurodegenerativas são um conjunto de afecções cerebrais devastadoras que cursam com senescência e morte neuronal. A doença de Alzheimer (DA), a principal causa de demência senil em todo o mundo, é uma doença neurodegenerativa caracterizada por uma perda progressiva da cognição e das funções superiores, devido a uma neurotoxicidade central difusa, putativamente atribuída a placas senis, compostas por peptídeos amilóides altamente tóxicos. Aqui apresento um novo e inovador modelo in vitro para a DA, baseado em cérebros de larvas de Drosophila melanogaster de terceiro instar. O modelo revelou-se adequado para a obtenção de imagens de células vivas, utilizando sondas fluorescentes que permitiram a coloração de membranas, núcleos, mitocôndria e lisossomos. Além disso, os resultados obtidos foram robustos e reprodutíveis. Os dados morfométricos indicam que a expressão incorreta de amilóide nos neurônios conduz a fenótipos estruturais distintos nas células observadas, favorecendo o alcance do modelo.Biblioteca Digitais de Teses e Dissertações da USPAlberici, Luciane CarlaLima, Carlos Antônio CoutoLopes, Caio Fábio Baêta2023-07-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttps://www.teses.usp.br/teses/disponiveis/17/17131/tde-10102023-112050/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPReter o conteúdo por motivos de patente, publicação e/ou direitos autoriais.info:eu-repo/semantics/openAccesseng2023-12-06T15:00:03Zoai:teses.usp.br:tde-10102023-112050Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212023-12-06T15:00:03Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Drosophila melanogaster primary cell cultures as tools for neurobiology: focusing on mitochondria and Alzheimer\'s disease Cultivos celulares primários de Drosophila melanogaster como ferramentas em neurobiologia: foco em mitocôndrias e doença de Alzheimer |
title |
Drosophila melanogaster primary cell cultures as tools for neurobiology: focusing on mitochondria and Alzheimer\'s disease |
spellingShingle |
Drosophila melanogaster primary cell cultures as tools for neurobiology: focusing on mitochondria and Alzheimer\'s disease Lopes, Caio Fábio Baêta Alzheimer's disease Culturas neuronais Doença de Alzheimer Drosophila melanogaster Drosophila melanogaster Mitochondria Mitocôndria Mitofagia Mitophagy Neuronal cultures |
title_short |
Drosophila melanogaster primary cell cultures as tools for neurobiology: focusing on mitochondria and Alzheimer\'s disease |
title_full |
Drosophila melanogaster primary cell cultures as tools for neurobiology: focusing on mitochondria and Alzheimer\'s disease |
title_fullStr |
Drosophila melanogaster primary cell cultures as tools for neurobiology: focusing on mitochondria and Alzheimer\'s disease |
title_full_unstemmed |
Drosophila melanogaster primary cell cultures as tools for neurobiology: focusing on mitochondria and Alzheimer\'s disease |
title_sort |
Drosophila melanogaster primary cell cultures as tools for neurobiology: focusing on mitochondria and Alzheimer\'s disease |
author |
Lopes, Caio Fábio Baêta |
author_facet |
Lopes, Caio Fábio Baêta |
author_role |
author |
dc.contributor.none.fl_str_mv |
Alberici, Luciane Carla Lima, Carlos Antônio Couto |
dc.contributor.author.fl_str_mv |
Lopes, Caio Fábio Baêta |
dc.subject.por.fl_str_mv |
Alzheimer's disease Culturas neuronais Doença de Alzheimer Drosophila melanogaster Drosophila melanogaster Mitochondria Mitocôndria Mitofagia Mitophagy Neuronal cultures |
topic |
Alzheimer's disease Culturas neuronais Doença de Alzheimer Drosophila melanogaster Drosophila melanogaster Mitochondria Mitocôndria Mitofagia Mitophagy Neuronal cultures |
description |
Brains are the most complex known objects of universe. Such a complexity emerges from structure and connectivity between neurons, their main building blocks. These are high polarized cells, shaped to capture, to process and to convey electrical stimuli via synapses, orchestrating uncountable discrete signals that give rise to sophisticated phenomena, as consciousness. Neurodegenerative diseases are a set of devastating brain affections that course with neuronal senescence and death. Alzheimer\'s disease (AD), the leading cause of senile dementia worldwide, is a neurodegenerative disease characterized by progessive loss of cogniton and higher functions, due to diffuse central neurotoxicity, putatively attributed to senile plaques, composed of toxic amyloid peptides. Here I introduce a new, innovative, in vitro model for AD, based on third instar Drosophila melanogaster larval brains. The model proved adequate for live cell imaging, using fluorescent probes that allowed membrane, nuclear, mitochondrial and lysosomal stainings. Moreover, results obtained were robust and reproducible. Morphometric data indicate that amyloid missexpression in neurons lead to distinct structural phenotypes on the observed cells, leveraging potential uses of the model. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.teses.usp.br/teses/disponiveis/17/17131/tde-10102023-112050/ |
url |
https://www.teses.usp.br/teses/disponiveis/17/17131/tde-10102023-112050/ |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
|
dc.rights.driver.fl_str_mv |
Reter o conteúdo por motivos de patente, publicação e/ou direitos autoriais. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Reter o conteúdo por motivos de patente, publicação e/ou direitos autoriais. |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
|
dc.publisher.none.fl_str_mv |
Biblioteca Digitais de Teses e Dissertações da USP |
publisher.none.fl_str_mv |
Biblioteca Digitais de Teses e Dissertações da USP |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da USP instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Biblioteca Digital de Teses e Dissertações da USP |
collection |
Biblioteca Digital de Teses e Dissertações da USP |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br |
_version_ |
1815256925128884224 |