Drosophila melanogaster primary cell cultures as tools for neurobiology: focusing on mitochondria and Alzheimer\'s disease

Detalhes bibliográficos
Autor(a) principal: Lopes, Caio Fábio Baêta
Data de Publicação: 2023
Tipo de documento: Tese
Idioma: eng
Título da fonte: Biblioteca Digital de Teses e Dissertações da USP
Texto Completo: https://www.teses.usp.br/teses/disponiveis/17/17131/tde-10102023-112050/
Resumo: Brains are the most complex known objects of universe. Such a complexity emerges from structure and connectivity between neurons, their main building blocks. These are high polarized cells, shaped to capture, to process and to convey electrical stimuli via synapses, orchestrating uncountable discrete signals that give rise to sophisticated phenomena, as consciousness. Neurodegenerative diseases are a set of devastating brain affections that course with neuronal senescence and death. Alzheimer\'s disease (AD), the leading cause of senile dementia worldwide, is a neurodegenerative disease characterized by progessive loss of cogniton and higher functions, due to diffuse central neurotoxicity, putatively attributed to senile plaques, composed of toxic amyloid peptides. Here I introduce a new, innovative, in vitro model for AD, based on third instar Drosophila melanogaster larval brains. The model proved adequate for live cell imaging, using fluorescent probes that allowed membrane, nuclear, mitochondrial and lysosomal stainings. Moreover, results obtained were robust and reproducible. Morphometric data indicate that amyloid missexpression in neurons lead to distinct structural phenotypes on the observed cells, leveraging potential uses of the model.
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spelling Drosophila melanogaster primary cell cultures as tools for neurobiology: focusing on mitochondria and Alzheimer\'s diseaseCultivos celulares primários de Drosophila melanogaster como ferramentas em neurobiologia: foco em mitocôndrias e doença de AlzheimerAlzheimer's diseaseCulturas neuronaisDoença de AlzheimerDrosophila melanogasterDrosophila melanogasterMitochondriaMitocôndriaMitofagiaMitophagyNeuronal culturesBrains are the most complex known objects of universe. Such a complexity emerges from structure and connectivity between neurons, their main building blocks. These are high polarized cells, shaped to capture, to process and to convey electrical stimuli via synapses, orchestrating uncountable discrete signals that give rise to sophisticated phenomena, as consciousness. Neurodegenerative diseases are a set of devastating brain affections that course with neuronal senescence and death. Alzheimer\'s disease (AD), the leading cause of senile dementia worldwide, is a neurodegenerative disease characterized by progessive loss of cogniton and higher functions, due to diffuse central neurotoxicity, putatively attributed to senile plaques, composed of toxic amyloid peptides. Here I introduce a new, innovative, in vitro model for AD, based on third instar Drosophila melanogaster larval brains. The model proved adequate for live cell imaging, using fluorescent probes that allowed membrane, nuclear, mitochondrial and lysosomal stainings. Moreover, results obtained were robust and reproducible. Morphometric data indicate that amyloid missexpression in neurons lead to distinct structural phenotypes on the observed cells, leveraging potential uses of the model.Cérebros são os objetos mais complexos do universo conhecido. Tal complexidade emerge da estrutura e conectividade entre neurônios, as suas principais células. Estes são altamente polarizados, moldados para captar, processar e transmitir estímulos elétricos através das sinapses, orquestrando inúmeros sinais discretos que dão origem a fenômenos sofisticados, como a consciência. As doenças neurodegenerativas são um conjunto de afecções cerebrais devastadoras que cursam com senescência e morte neuronal. A doença de Alzheimer (DA), a principal causa de demência senil em todo o mundo, é uma doença neurodegenerativa caracterizada por uma perda progressiva da cognição e das funções superiores, devido a uma neurotoxicidade central difusa, putativamente atribuída a placas senis, compostas por peptídeos amilóides altamente tóxicos. Aqui apresento um novo e inovador modelo in vitro para a DA, baseado em cérebros de larvas de Drosophila melanogaster de terceiro instar. O modelo revelou-se adequado para a obtenção de imagens de células vivas, utilizando sondas fluorescentes que permitiram a coloração de membranas, núcleos, mitocôndria e lisossomos. Além disso, os resultados obtidos foram robustos e reprodutíveis. Os dados morfométricos indicam que a expressão incorreta de amilóide nos neurônios conduz a fenótipos estruturais distintos nas células observadas, favorecendo o alcance do modelo.Biblioteca Digitais de Teses e Dissertações da USPAlberici, Luciane CarlaLima, Carlos Antônio CoutoLopes, Caio Fábio Baêta2023-07-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttps://www.teses.usp.br/teses/disponiveis/17/17131/tde-10102023-112050/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPReter o conteúdo por motivos de patente, publicação e/ou direitos autoriais.info:eu-repo/semantics/openAccesseng2023-12-06T15:00:03Zoai:teses.usp.br:tde-10102023-112050Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212023-12-06T15:00:03Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Drosophila melanogaster primary cell cultures as tools for neurobiology: focusing on mitochondria and Alzheimer\'s disease
Cultivos celulares primários de Drosophila melanogaster como ferramentas em neurobiologia: foco em mitocôndrias e doença de Alzheimer
title Drosophila melanogaster primary cell cultures as tools for neurobiology: focusing on mitochondria and Alzheimer\'s disease
spellingShingle Drosophila melanogaster primary cell cultures as tools for neurobiology: focusing on mitochondria and Alzheimer\'s disease
Lopes, Caio Fábio Baêta
Alzheimer's disease
Culturas neuronais
Doença de Alzheimer
Drosophila melanogaster
Drosophila melanogaster
Mitochondria
Mitocôndria
Mitofagia
Mitophagy
Neuronal cultures
title_short Drosophila melanogaster primary cell cultures as tools for neurobiology: focusing on mitochondria and Alzheimer\'s disease
title_full Drosophila melanogaster primary cell cultures as tools for neurobiology: focusing on mitochondria and Alzheimer\'s disease
title_fullStr Drosophila melanogaster primary cell cultures as tools for neurobiology: focusing on mitochondria and Alzheimer\'s disease
title_full_unstemmed Drosophila melanogaster primary cell cultures as tools for neurobiology: focusing on mitochondria and Alzheimer\'s disease
title_sort Drosophila melanogaster primary cell cultures as tools for neurobiology: focusing on mitochondria and Alzheimer\'s disease
author Lopes, Caio Fábio Baêta
author_facet Lopes, Caio Fábio Baêta
author_role author
dc.contributor.none.fl_str_mv Alberici, Luciane Carla
Lima, Carlos Antônio Couto
dc.contributor.author.fl_str_mv Lopes, Caio Fábio Baêta
dc.subject.por.fl_str_mv Alzheimer's disease
Culturas neuronais
Doença de Alzheimer
Drosophila melanogaster
Drosophila melanogaster
Mitochondria
Mitocôndria
Mitofagia
Mitophagy
Neuronal cultures
topic Alzheimer's disease
Culturas neuronais
Doença de Alzheimer
Drosophila melanogaster
Drosophila melanogaster
Mitochondria
Mitocôndria
Mitofagia
Mitophagy
Neuronal cultures
description Brains are the most complex known objects of universe. Such a complexity emerges from structure and connectivity between neurons, their main building blocks. These are high polarized cells, shaped to capture, to process and to convey electrical stimuli via synapses, orchestrating uncountable discrete signals that give rise to sophisticated phenomena, as consciousness. Neurodegenerative diseases are a set of devastating brain affections that course with neuronal senescence and death. Alzheimer\'s disease (AD), the leading cause of senile dementia worldwide, is a neurodegenerative disease characterized by progessive loss of cogniton and higher functions, due to diffuse central neurotoxicity, putatively attributed to senile plaques, composed of toxic amyloid peptides. Here I introduce a new, innovative, in vitro model for AD, based on third instar Drosophila melanogaster larval brains. The model proved adequate for live cell imaging, using fluorescent probes that allowed membrane, nuclear, mitochondrial and lysosomal stainings. Moreover, results obtained were robust and reproducible. Morphometric data indicate that amyloid missexpression in neurons lead to distinct structural phenotypes on the observed cells, leveraging potential uses of the model.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-07
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.teses.usp.br/teses/disponiveis/17/17131/tde-10102023-112050/
url https://www.teses.usp.br/teses/disponiveis/17/17131/tde-10102023-112050/
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv
dc.rights.driver.fl_str_mv Reter o conteúdo por motivos de patente, publicação e/ou direitos autoriais.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Reter o conteúdo por motivos de patente, publicação e/ou direitos autoriais.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv
dc.publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
dc.source.none.fl_str_mv
reponame:Biblioteca Digital de Teses e Dissertações da USP
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Biblioteca Digital de Teses e Dissertações da USP
collection Biblioteca Digital de Teses e Dissertações da USP
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)
repository.mail.fl_str_mv virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br
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