How 10-MDP functional phosphate monomer and 2% digluconate chlorhexidine solution interact on sound, artificial carious or eroded dentin substrates

Detalhes bibliográficos
Autor(a) principal: Jacomine, Juliana Carvalho
Data de Publicação: 2019
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Biblioteca Digital de Teses e Dissertações da USP
Texto Completo: http://www.teses.usp.br/teses/disponiveis/25/25148/tde-05092019-202550/
Resumo: Objective: Carious and eroded dentin represent common modified dental substrates, which mostly require restorative procedures. MDP-based dentin bonding system (DBS) and digluconate chlorhexidine (CHX) are presented as promising agents to minimize intrinsic degradation of the resin-dentin interface by interaction with calcium (Ca). Therefore, the purpose of this study was to explore the possible interaction between MDP in a universal adhesive system with 10-MDP in self-etching mode and CHX on substrates artificially modified by caries and erosion, through microtensile bond strength (TBS). Additional characterization of the adhesive interface was performed by SEM/EDS analyses. Material and methods: Flat dentin surfaces were obtained from 120 specimens (n=20/group) prepared from extracted sound human third molars and randomly divided into three groups according to the dentin substrate: sound-control (S), artificial carious (C) and artificial eroded (E). Half of these specimens were pretreated with distilled water (W) and other half with 2% CHX, constituting 6 groups: SW, S-CHX, C-W, C-CHX, E-W, E-CHX. After, all the specimens were restored with a universal adhesive system (Apder Single Bond Universal) using self-etching mode and two increments of composite resin (Filtek Z-350), following manufacturers instructions. Slices (0.8mm) were obtained to SEM analysis and beams (0.64mm²) were obtained and evaluated by EDS analysis and TBS in universal testing machine (500N/ 0.5mm/min) after 24 hours and 6 months. Data was statistically analyzed by two-way ANOVA and Tukey tests (p<0.05). Results: Substrate type was a statistically significant factor (p<0.0001), whereas the pretreatment (p=0.189), time (p=0.337) and the interaction between three factors (p=0.452) were not significant. Conclusion: Carious and eroded dentin substrates negatively interfered on the bond strength of an MDP- based universal adhesive systems, regardless its use with CHX. Likely, the reduction of available calcium from these substrates impaired the effectiveness of this system.
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spelling How 10-MDP functional phosphate monomer and 2% digluconate chlorhexidine solution interact on sound, artificial carious or eroded dentin substratesComo o monômero funcional fosfatado 10-MDP e a solução de digluconato de clorexidina a 2% interagem em substratos dentinários hígido, cariado e erodido artificialmenteAdesivos dentináriosCárieDental cariesDentin-bonding agentsErosãoErosionObjective: Carious and eroded dentin represent common modified dental substrates, which mostly require restorative procedures. MDP-based dentin bonding system (DBS) and digluconate chlorhexidine (CHX) are presented as promising agents to minimize intrinsic degradation of the resin-dentin interface by interaction with calcium (Ca). Therefore, the purpose of this study was to explore the possible interaction between MDP in a universal adhesive system with 10-MDP in self-etching mode and CHX on substrates artificially modified by caries and erosion, through microtensile bond strength (TBS). Additional characterization of the adhesive interface was performed by SEM/EDS analyses. Material and methods: Flat dentin surfaces were obtained from 120 specimens (n=20/group) prepared from extracted sound human third molars and randomly divided into three groups according to the dentin substrate: sound-control (S), artificial carious (C) and artificial eroded (E). Half of these specimens were pretreated with distilled water (W) and other half with 2% CHX, constituting 6 groups: SW, S-CHX, C-W, C-CHX, E-W, E-CHX. After, all the specimens were restored with a universal adhesive system (Apder Single Bond Universal) using self-etching mode and two increments of composite resin (Filtek Z-350), following manufacturers instructions. Slices (0.8mm) were obtained to SEM analysis and beams (0.64mm²) were obtained and evaluated by EDS analysis and TBS in universal testing machine (500N/ 0.5mm/min) after 24 hours and 6 months. Data was statistically analyzed by two-way ANOVA and Tukey tests (p<0.05). Results: Substrate type was a statistically significant factor (p<0.0001), whereas the pretreatment (p=0.189), time (p=0.337) and the interaction between three factors (p=0.452) were not significant. Conclusion: Carious and eroded dentin substrates negatively interfered on the bond strength of an MDP- based universal adhesive systems, regardless its use with CHX. Likely, the reduction of available calcium from these substrates impaired the effectiveness of this system.Objetivo: Substratos dentinários cariados e erodidos representam os substratos modificados mais comuns na prática clínica. Sistemas adesivos baseados em MDP e CHX são apresentados como promissores agentes para minimizar a degradação intrínseca da interface resina-dentina devido a suas interações com cálcio. O objetivo deste estudo foi explorar a possível interação entre MDP em um sistema adesivo universal com 10-MDP em modo autocondicionante e CHX em substratos artificialmente modificados por cárie e erosão através da resistência de união (TBS). Caracterizações adicionais das interfaces adesivas foram realizadas por análise em MEV/EDS. Material e métodos: Cento e vinte terceiros molares hígidos foram preparados e randomizados em três grupos de avaliação de acordo com a condição dentinária (n=20/grupo): hígida (controle), cariada e erodida. Metade dos espécimes foram tratados com água (controle) e a outra metade com solução aquosa de CHX a 2%. Todos os espécimes foram restaurados com sistema adesivo Adper Single Bond Universal no modo autocondicionante e resina composta Filtek Z250, seguindo as instruções do fabricante. Fatias (0,8mm) foram obtidas paras as análises em MEV e, na sequência, palitos (0,64mm²) para as avaliações de EDS e TBS em máquina de ensaios universal (500N/0,5mm/min) depois de 24 horas e 6 meses. Os dados foram submetidos à análise estatística (ANOVA e teste Tukey (p<0,05). Resultados: O tipo de substrato foi o único fator estatisticamente significante (p<0,0001), enquanto o pré-tratamento (p=0,189), tempo (p=0,337) e a interação entre os três fatores (p=0,452) não foram significantes. Conclusão: Substratos dentinários cariados e erodidos interferem negativamente na resistência de união de sistemas adesivos universais baseados em MDP, independente do uso de CHX. Provavelmente a redução da disponibilidade de cálcio nesses substratos prejudica a efetividade deste sistema.Biblioteca Digitais de Teses e Dissertações da USPWang, LindaJacomine, Juliana Carvalho2019-03-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://www.teses.usp.br/teses/disponiveis/25/25148/tde-05092019-202550/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2019-11-08T20:38:00Zoai:teses.usp.br:tde-05092019-202550Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212019-11-08T20:38Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv How 10-MDP functional phosphate monomer and 2% digluconate chlorhexidine solution interact on sound, artificial carious or eroded dentin substrates
Como o monômero funcional fosfatado 10-MDP e a solução de digluconato de clorexidina a 2% interagem em substratos dentinários hígido, cariado e erodido artificialmente
title How 10-MDP functional phosphate monomer and 2% digluconate chlorhexidine solution interact on sound, artificial carious or eroded dentin substrates
spellingShingle How 10-MDP functional phosphate monomer and 2% digluconate chlorhexidine solution interact on sound, artificial carious or eroded dentin substrates
Jacomine, Juliana Carvalho
Adesivos dentinários
Cárie
Dental caries
Dentin-bonding agents
Erosão
Erosion
title_short How 10-MDP functional phosphate monomer and 2% digluconate chlorhexidine solution interact on sound, artificial carious or eroded dentin substrates
title_full How 10-MDP functional phosphate monomer and 2% digluconate chlorhexidine solution interact on sound, artificial carious or eroded dentin substrates
title_fullStr How 10-MDP functional phosphate monomer and 2% digluconate chlorhexidine solution interact on sound, artificial carious or eroded dentin substrates
title_full_unstemmed How 10-MDP functional phosphate monomer and 2% digluconate chlorhexidine solution interact on sound, artificial carious or eroded dentin substrates
title_sort How 10-MDP functional phosphate monomer and 2% digluconate chlorhexidine solution interact on sound, artificial carious or eroded dentin substrates
author Jacomine, Juliana Carvalho
author_facet Jacomine, Juliana Carvalho
author_role author
dc.contributor.none.fl_str_mv Wang, Linda
dc.contributor.author.fl_str_mv Jacomine, Juliana Carvalho
dc.subject.por.fl_str_mv Adesivos dentinários
Cárie
Dental caries
Dentin-bonding agents
Erosão
Erosion
topic Adesivos dentinários
Cárie
Dental caries
Dentin-bonding agents
Erosão
Erosion
description Objective: Carious and eroded dentin represent common modified dental substrates, which mostly require restorative procedures. MDP-based dentin bonding system (DBS) and digluconate chlorhexidine (CHX) are presented as promising agents to minimize intrinsic degradation of the resin-dentin interface by interaction with calcium (Ca). Therefore, the purpose of this study was to explore the possible interaction between MDP in a universal adhesive system with 10-MDP in self-etching mode and CHX on substrates artificially modified by caries and erosion, through microtensile bond strength (TBS). Additional characterization of the adhesive interface was performed by SEM/EDS analyses. Material and methods: Flat dentin surfaces were obtained from 120 specimens (n=20/group) prepared from extracted sound human third molars and randomly divided into three groups according to the dentin substrate: sound-control (S), artificial carious (C) and artificial eroded (E). Half of these specimens were pretreated with distilled water (W) and other half with 2% CHX, constituting 6 groups: SW, S-CHX, C-W, C-CHX, E-W, E-CHX. After, all the specimens were restored with a universal adhesive system (Apder Single Bond Universal) using self-etching mode and two increments of composite resin (Filtek Z-350), following manufacturers instructions. Slices (0.8mm) were obtained to SEM analysis and beams (0.64mm²) were obtained and evaluated by EDS analysis and TBS in universal testing machine (500N/ 0.5mm/min) after 24 hours and 6 months. Data was statistically analyzed by two-way ANOVA and Tukey tests (p<0.05). Results: Substrate type was a statistically significant factor (p<0.0001), whereas the pretreatment (p=0.189), time (p=0.337) and the interaction between three factors (p=0.452) were not significant. Conclusion: Carious and eroded dentin substrates negatively interfered on the bond strength of an MDP- based universal adhesive systems, regardless its use with CHX. Likely, the reduction of available calcium from these substrates impaired the effectiveness of this system.
publishDate 2019
dc.date.none.fl_str_mv 2019-03-22
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dc.rights.driver.fl_str_mv Liberar o conteúdo para acesso público.
info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
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