Role of TLR4 in endoplasmic reticulum stress induced by physical exercise in skeletal muscle

Detalhes bibliográficos
Autor(a) principal: Marafon, Bruno Brieda
Data de Publicação: 2022
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Biblioteca Digital de Teses e Dissertações da USP
Texto Completo: https://www.teses.usp.br/teses/disponiveis/109/109131/tde-19092022-073341/
Resumo: Many conditions can induce endoplasmic reticulum (ER) stress, such as inflammation and physical exercise. Toll-like Receptor 4 (TLR4) can trigger inflammation and ER stress events. However, there are still no data in the literature regarding the role of TLR4 in ER stress during exercise in skeletal muscle. Therefore, the current investigation aimed to verify the responses of ER stress markers in wild-type (WT) and Tlr4 global knockout (KO) mice after acute and chronic physical exercise protocols. Eight-week-old male WT and KO mice were submitted to acute (moderate or high intensity) and chronic (4-week protocol) treadmill exercise. Under basal conditions, KO mice showed lower performance in the rotarod test, and increased eIF2α protein phosphorylation compared to WT animals. Acute moderate-intensity exercise increased BiP and CHOP protein in the WT group. After the acute high-intensity exercise, there was an increase in Casp3 and Ddit3 mRNA for the KO mice. Acute exercise increased the cleaved Caspase-3/Caspase-3 in the KO group regardless of exercise intensity. In response to chronic exercise, the KO group showed no improvement in any performance evaluation. The 4-week chronic protocol did not generate changes in CHOP, p-eIF2α/eIF2α, and cleaved Caspase-3/Caspase-3 ratio but reduced BiP protein compared to the KO-Sedentary group. These results demonstrate that the global deletion of Tlr4 seems to protect the mice against ER stress but decreases their performance. The cleaved Caspase-3/Caspase-3 ratio may be activated by another pathway other than ER stress in Tlr4 KO animals.
id USP_e6477c5198568502df0da43a5728ea20
oai_identifier_str oai:teses.usp.br:tde-19092022-073341
network_acronym_str USP
network_name_str Biblioteca Digital de Teses e Dissertações da USP
repository_id_str 2721
spelling Role of TLR4 in endoplasmic reticulum stress induced by physical exercise in skeletal musclePapel do TLR4 no estresse do retículo endoplasmático induzido pelo exercício físico no músculo esqueléticoApoptoseApoptosisExercício físicoKnockout modelModelo NocautePhysical exerciseReceptor tipo TollTLR4TLR4Toll-like receptorMany conditions can induce endoplasmic reticulum (ER) stress, such as inflammation and physical exercise. Toll-like Receptor 4 (TLR4) can trigger inflammation and ER stress events. However, there are still no data in the literature regarding the role of TLR4 in ER stress during exercise in skeletal muscle. Therefore, the current investigation aimed to verify the responses of ER stress markers in wild-type (WT) and Tlr4 global knockout (KO) mice after acute and chronic physical exercise protocols. Eight-week-old male WT and KO mice were submitted to acute (moderate or high intensity) and chronic (4-week protocol) treadmill exercise. Under basal conditions, KO mice showed lower performance in the rotarod test, and increased eIF2α protein phosphorylation compared to WT animals. Acute moderate-intensity exercise increased BiP and CHOP protein in the WT group. After the acute high-intensity exercise, there was an increase in Casp3 and Ddit3 mRNA for the KO mice. Acute exercise increased the cleaved Caspase-3/Caspase-3 in the KO group regardless of exercise intensity. In response to chronic exercise, the KO group showed no improvement in any performance evaluation. The 4-week chronic protocol did not generate changes in CHOP, p-eIF2α/eIF2α, and cleaved Caspase-3/Caspase-3 ratio but reduced BiP protein compared to the KO-Sedentary group. These results demonstrate that the global deletion of Tlr4 seems to protect the mice against ER stress but decreases their performance. The cleaved Caspase-3/Caspase-3 ratio may be activated by another pathway other than ER stress in Tlr4 KO animals.Muitas condições podem induzir o estresse do retículo endoplasmático (RE), como inflamação e exercício físico, por exemplo. O receptor tipo Toll 4 (TLR4) pode desencadear inflamação e eventos de estresse do RE. No entanto, ainda não existem dados na literatura sobre o papel do TLR4 no estresse do RE durante o exercício no músculo esquelético. Portanto, o presente estudo teve como objetivo investigar e verificar as respostas dos marcadores de estresse de RE em camundongos do tipo selvagem (WT) e Tlr4 nocaute global (KO) após protocolos de exercício físico agudo e crônico. Camundongos machos WT e KO com oito semanas de idade foram submetidos a exercícios agudos (moderada ou alta intensidade) e crônicos (protocolo de 4 semanas). Em condições basais, os camundongos KO apresentaram desempenho inferior no teste do rotarod e maior fosforilação da proteína eIF2α em comparação aos animais WT. O exercício agudo de intensidade moderada aumentou a BiP e a proteína CHOP no grupo WT. Após o exercício agudo de alta intensidade, houve aumento nos níveis de RNAm de Casp3 e Ddit3 para os camundongos KO. O exercício agudo aumentou a Caspase-3 clivada/Caspase-3 no grupo KO, independentemente da intensidade do exercício. Em resposta ao exercício crônico, o grupo KO não apresentou melhora em nenhuma avaliação de desempenho. O protocolo crônico de 4 semanas não gerou alterações na CHOP, na razão p-eIF2α/eIF2α e Caspase-3 clivada/Caspase-3, mas reduziu a proteína BiP em relação ao grupo KO-Sedentário. Esses resultados demonstram que a deleção global de Tlr4 parece proteger os camundongos contra o estresse do RE, mas diminui seu desempenho. Ainda, parece que a razão Caspase-3 clivada/Caspase-3 pode ser ativada por outra via diferente do estresse de RE em animais Tlr4 KO.Biblioteca Digitais de Teses e Dissertações da USPSilva, Adelino Sanchez Ramos daMarafon, Bruno Brieda2022-09-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://www.teses.usp.br/teses/disponiveis/109/109131/tde-19092022-073341/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2022-09-28T12:08:49Zoai:teses.usp.br:tde-19092022-073341Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212022-09-28T12:08:49Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Role of TLR4 in endoplasmic reticulum stress induced by physical exercise in skeletal muscle
Papel do TLR4 no estresse do retículo endoplasmático induzido pelo exercício físico no músculo esquelético
title Role of TLR4 in endoplasmic reticulum stress induced by physical exercise in skeletal muscle
spellingShingle Role of TLR4 in endoplasmic reticulum stress induced by physical exercise in skeletal muscle
Marafon, Bruno Brieda
Apoptose
Apoptosis
Exercício físico
Knockout model
Modelo Nocaute
Physical exercise
Receptor tipo Toll
TLR4
TLR4
Toll-like receptor
title_short Role of TLR4 in endoplasmic reticulum stress induced by physical exercise in skeletal muscle
title_full Role of TLR4 in endoplasmic reticulum stress induced by physical exercise in skeletal muscle
title_fullStr Role of TLR4 in endoplasmic reticulum stress induced by physical exercise in skeletal muscle
title_full_unstemmed Role of TLR4 in endoplasmic reticulum stress induced by physical exercise in skeletal muscle
title_sort Role of TLR4 in endoplasmic reticulum stress induced by physical exercise in skeletal muscle
author Marafon, Bruno Brieda
author_facet Marafon, Bruno Brieda
author_role author
dc.contributor.none.fl_str_mv Silva, Adelino Sanchez Ramos da
dc.contributor.author.fl_str_mv Marafon, Bruno Brieda
dc.subject.por.fl_str_mv Apoptose
Apoptosis
Exercício físico
Knockout model
Modelo Nocaute
Physical exercise
Receptor tipo Toll
TLR4
TLR4
Toll-like receptor
topic Apoptose
Apoptosis
Exercício físico
Knockout model
Modelo Nocaute
Physical exercise
Receptor tipo Toll
TLR4
TLR4
Toll-like receptor
description Many conditions can induce endoplasmic reticulum (ER) stress, such as inflammation and physical exercise. Toll-like Receptor 4 (TLR4) can trigger inflammation and ER stress events. However, there are still no data in the literature regarding the role of TLR4 in ER stress during exercise in skeletal muscle. Therefore, the current investigation aimed to verify the responses of ER stress markers in wild-type (WT) and Tlr4 global knockout (KO) mice after acute and chronic physical exercise protocols. Eight-week-old male WT and KO mice were submitted to acute (moderate or high intensity) and chronic (4-week protocol) treadmill exercise. Under basal conditions, KO mice showed lower performance in the rotarod test, and increased eIF2α protein phosphorylation compared to WT animals. Acute moderate-intensity exercise increased BiP and CHOP protein in the WT group. After the acute high-intensity exercise, there was an increase in Casp3 and Ddit3 mRNA for the KO mice. Acute exercise increased the cleaved Caspase-3/Caspase-3 in the KO group regardless of exercise intensity. In response to chronic exercise, the KO group showed no improvement in any performance evaluation. The 4-week chronic protocol did not generate changes in CHOP, p-eIF2α/eIF2α, and cleaved Caspase-3/Caspase-3 ratio but reduced BiP protein compared to the KO-Sedentary group. These results demonstrate that the global deletion of Tlr4 seems to protect the mice against ER stress but decreases their performance. The cleaved Caspase-3/Caspase-3 ratio may be activated by another pathway other than ER stress in Tlr4 KO animals.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-12
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.teses.usp.br/teses/disponiveis/109/109131/tde-19092022-073341/
url https://www.teses.usp.br/teses/disponiveis/109/109131/tde-19092022-073341/
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv
dc.rights.driver.fl_str_mv Liberar o conteúdo para acesso público.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Liberar o conteúdo para acesso público.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv
dc.publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
dc.source.none.fl_str_mv
reponame:Biblioteca Digital de Teses e Dissertações da USP
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Biblioteca Digital de Teses e Dissertações da USP
collection Biblioteca Digital de Teses e Dissertações da USP
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)
repository.mail.fl_str_mv virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br
_version_ 1815257132474302464