Evaluation of ultrastructural changes and cell death on Leishmania amazonensis promastigote forms induced by a new coordinated complex Co (II) / Avaliação de alterações ultraestruturais e morte celular em formas promastigotas de Leishmania amazonensis induzidas por um novo complexo coordenação Co (II)

• Autor(a) principal: Lorete-Terra, Aline Rodrigues de Mesquita
• Data de Publicação: 2021
• Outros Autores: da Matta, Renato Augusto, Filho, Celso Barbosa de Santanna, Fernandes, Christiane, Junior, Adolfo Horn, de Souza, Wanderley, Fortes, Fabio da Silva de Azevedo, Seabra, Sérgio Henrique
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista Veras
• Texto Completo: https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/25305
• topic: Leishmania amazonensis; Co(II) complex; TEM; autophagy.

Leishmaniasis are diseases caused by protozoa of the genus Leishmania and transmitted by vectors of the phlebotomine subfamily. In Brazil, the species Leishmania amazonensis is the etiological agent of the diffuse cutaneous form. Currently, the treatment employed is associated with several side effects, which stimulated the development of new alternatives for the treatment of this important neglected disease. Thus, metallocomplexes appear as a new alternative for antiparasitic therapy. Its action has been evaluated on different species of parasites of the Trypanosomatidae family, including species of the genus Leishmania. In this work, we evaluated the effect of a new Co (II) complex, against promastigotes of L. amazonensis of strain WHOM / BR / 75 / Josefa. Transmission electron microscopy (TEM) was performed to evaluate the parasite's ultrastructure and confocal laser microscopy to check for death by autophagy of the parasite. The complex was able to induce ultrastructural changes in the parasite, such as the formation of autophagic vacuoles near the pouch region flagellar and myelin figure formations. Finally, tests with anti-LC3B labeling indicated the possible death of the parasite by autophagy.