Chemical characterization, antioxidant, cytotoxic, and antibacterial activities of Eugenia uniflora L. and Psidium cattleianum Sabine essential oils against Klebsiella pneumoniae and Acinetobacter baumannii/ Caracterização química, antioxidante, citotóxica e antibacteriana dos óleos essenciais de Eugenia uniflora L. e Psidium cattleianum Sabine contra Klebsiella pneumoniae e Acinetobacter baumannii

Detalhes bibliográficos
Autor(a) principal: Garcia, Marcelle Oliveira
Data de Publicação: 2021
Outros Autores: Da Cunha, Kamila Furtado, Allend, Suzane Olachea, Da Silva, Mirian Elert, De Santi, Ivandra Ignês, Freitag, Rogério Antonio, Hübner, Silvia De Oliveira Rodrigues, Hartwig, Daiane Drawanz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Veras
Texto Completo: https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/26521
Resumo: INTRODUCTION: Eugenia uniflora L. and Psidium cattleianum Sabine essential oils (EO) can be biologically active and serve as novel sources of antibiotics for Klebsiella pneumoniae and Acinetobacter baumannii bacteria.METHODS: The EO of E. uniflora L. (EOE) and P. cattleianum Sabine (EOP) were extracted from de leaves of the plants and chemically characterized by gas chromatography-mass spectrometry (GC-MS). The antioxidant potential was evaluated by the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) technique and the cytotoxicity evaluated in mammalian VERO cell line by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The antibacterial activity of EOE and EOP was assessed by the broth microdilution method.RESULTS: The major compounds of EOE were benzofuran (24.38%), germacrene B (20.12%), ?-elemene (9.33%) and ?-cubebene (8.55%), and for EOP were ?-pinene (24.25%), ?-caryophyllene (20.45%), and eucalyptol (10.43%). Both EO tested showed low antioxidant effect and dose-dependent cytotoxicity in VERO cell line. The EOE was less toxic for the mammalian cells with an IC50 (half maximal inhibitory concentration) index of 75.0 mg.mL-1. The EOP and EOE were more active against A. baumannii, with a minimum inhibitory concentration (MIC) of 14.0 and 56.0 mg.mL-1 and a minimal bactericide concentration (MBC) of 14.0 and 112.0 mg.mL-1, respectively.  CONCLUSIONS: Although EOE and EOP have low antioxidant and antibacterial activity against A. baumanni and K. pneumoniae new approaches can be applied to improve this effect in vivo.   
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spelling Chemical characterization, antioxidant, cytotoxic, and antibacterial activities of Eugenia uniflora L. and Psidium cattleianum Sabine essential oils against Klebsiella pneumoniae and Acinetobacter baumannii/ Caracterização química, antioxidante, citotóxica e antibacteriana dos óleos essenciais de Eugenia uniflora L. e Psidium cattleianum Sabine contra Klebsiella pneumoniae e Acinetobacter baumanniiPitangaAraçáGram-negative bacteriaMyrtaceae.INTRODUCTION: Eugenia uniflora L. and Psidium cattleianum Sabine essential oils (EO) can be biologically active and serve as novel sources of antibiotics for Klebsiella pneumoniae and Acinetobacter baumannii bacteria.METHODS: The EO of E. uniflora L. (EOE) and P. cattleianum Sabine (EOP) were extracted from de leaves of the plants and chemically characterized by gas chromatography-mass spectrometry (GC-MS). The antioxidant potential was evaluated by the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) technique and the cytotoxicity evaluated in mammalian VERO cell line by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The antibacterial activity of EOE and EOP was assessed by the broth microdilution method.RESULTS: The major compounds of EOE were benzofuran (24.38%), germacrene B (20.12%), ?-elemene (9.33%) and ?-cubebene (8.55%), and for EOP were ?-pinene (24.25%), ?-caryophyllene (20.45%), and eucalyptol (10.43%). Both EO tested showed low antioxidant effect and dose-dependent cytotoxicity in VERO cell line. The EOE was less toxic for the mammalian cells with an IC50 (half maximal inhibitory concentration) index of 75.0 mg.mL-1. The EOP and EOE were more active against A. baumannii, with a minimum inhibitory concentration (MIC) of 14.0 and 56.0 mg.mL-1 and a minimal bactericide concentration (MBC) of 14.0 and 112.0 mg.mL-1, respectively.  CONCLUSIONS: Although EOE and EOP have low antioxidant and antibacterial activity against A. baumanni and K. pneumoniae new approaches can be applied to improve this effect in vivo.   Brazilian Journals Publicações de Periódicos e Editora Ltda.2021-03-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/2652110.34117/bjdv7n3-437Brazilian Journal of Development; Vol. 7 No. 3 (2021); 27276-27294Brazilian Journal of Development; Vol. 7 Núm. 3 (2021); 27276-27294Brazilian Journal of Development; v. 7 n. 3 (2021); 27276-272942525-8761reponame:Revista Verasinstname:Instituto Superior de Educação Vera Cruz (VeraCruz)instacron:VERACRUZenghttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/26521/21567Copyright (c) 2021 Brazilian Journal of Developmentinfo:eu-repo/semantics/openAccessGarcia, Marcelle OliveiraDa Cunha, Kamila FurtadoAllend, Suzane OlacheaDa Silva, Mirian ElertDe Santi, Ivandra IgnêsFreitag, Rogério AntonioHübner, Silvia De Oliveira RodriguesHartwig, Daiane Drawanz2022-03-29T12:15:40Zoai:ojs2.ojs.brazilianjournals.com.br:article/26521Revistahttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/PRIhttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/oai||revistaveras@veracruz.edu.br2236-57292236-5729opendoar:2024-10-15T16:14:21.829020Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz)false
dc.title.none.fl_str_mv Chemical characterization, antioxidant, cytotoxic, and antibacterial activities of Eugenia uniflora L. and Psidium cattleianum Sabine essential oils against Klebsiella pneumoniae and Acinetobacter baumannii/ Caracterização química, antioxidante, citotóxica e antibacteriana dos óleos essenciais de Eugenia uniflora L. e Psidium cattleianum Sabine contra Klebsiella pneumoniae e Acinetobacter baumannii
title Chemical characterization, antioxidant, cytotoxic, and antibacterial activities of Eugenia uniflora L. and Psidium cattleianum Sabine essential oils against Klebsiella pneumoniae and Acinetobacter baumannii/ Caracterização química, antioxidante, citotóxica e antibacteriana dos óleos essenciais de Eugenia uniflora L. e Psidium cattleianum Sabine contra Klebsiella pneumoniae e Acinetobacter baumannii
spellingShingle Chemical characterization, antioxidant, cytotoxic, and antibacterial activities of Eugenia uniflora L. and Psidium cattleianum Sabine essential oils against Klebsiella pneumoniae and Acinetobacter baumannii/ Caracterização química, antioxidante, citotóxica e antibacteriana dos óleos essenciais de Eugenia uniflora L. e Psidium cattleianum Sabine contra Klebsiella pneumoniae e Acinetobacter baumannii
Garcia, Marcelle Oliveira
Pitanga
Araçá
Gram-negative bacteria
Myrtaceae.
title_short Chemical characterization, antioxidant, cytotoxic, and antibacterial activities of Eugenia uniflora L. and Psidium cattleianum Sabine essential oils against Klebsiella pneumoniae and Acinetobacter baumannii/ Caracterização química, antioxidante, citotóxica e antibacteriana dos óleos essenciais de Eugenia uniflora L. e Psidium cattleianum Sabine contra Klebsiella pneumoniae e Acinetobacter baumannii
title_full Chemical characterization, antioxidant, cytotoxic, and antibacterial activities of Eugenia uniflora L. and Psidium cattleianum Sabine essential oils against Klebsiella pneumoniae and Acinetobacter baumannii/ Caracterização química, antioxidante, citotóxica e antibacteriana dos óleos essenciais de Eugenia uniflora L. e Psidium cattleianum Sabine contra Klebsiella pneumoniae e Acinetobacter baumannii
title_fullStr Chemical characterization, antioxidant, cytotoxic, and antibacterial activities of Eugenia uniflora L. and Psidium cattleianum Sabine essential oils against Klebsiella pneumoniae and Acinetobacter baumannii/ Caracterização química, antioxidante, citotóxica e antibacteriana dos óleos essenciais de Eugenia uniflora L. e Psidium cattleianum Sabine contra Klebsiella pneumoniae e Acinetobacter baumannii
title_full_unstemmed Chemical characterization, antioxidant, cytotoxic, and antibacterial activities of Eugenia uniflora L. and Psidium cattleianum Sabine essential oils against Klebsiella pneumoniae and Acinetobacter baumannii/ Caracterização química, antioxidante, citotóxica e antibacteriana dos óleos essenciais de Eugenia uniflora L. e Psidium cattleianum Sabine contra Klebsiella pneumoniae e Acinetobacter baumannii
title_sort Chemical characterization, antioxidant, cytotoxic, and antibacterial activities of Eugenia uniflora L. and Psidium cattleianum Sabine essential oils against Klebsiella pneumoniae and Acinetobacter baumannii/ Caracterização química, antioxidante, citotóxica e antibacteriana dos óleos essenciais de Eugenia uniflora L. e Psidium cattleianum Sabine contra Klebsiella pneumoniae e Acinetobacter baumannii
author Garcia, Marcelle Oliveira
author_facet Garcia, Marcelle Oliveira
Da Cunha, Kamila Furtado
Allend, Suzane Olachea
Da Silva, Mirian Elert
De Santi, Ivandra Ignês
Freitag, Rogério Antonio
Hübner, Silvia De Oliveira Rodrigues
Hartwig, Daiane Drawanz
author_role author
author2 Da Cunha, Kamila Furtado
Allend, Suzane Olachea
Da Silva, Mirian Elert
De Santi, Ivandra Ignês
Freitag, Rogério Antonio
Hübner, Silvia De Oliveira Rodrigues
Hartwig, Daiane Drawanz
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Garcia, Marcelle Oliveira
Da Cunha, Kamila Furtado
Allend, Suzane Olachea
Da Silva, Mirian Elert
De Santi, Ivandra Ignês
Freitag, Rogério Antonio
Hübner, Silvia De Oliveira Rodrigues
Hartwig, Daiane Drawanz
dc.subject.por.fl_str_mv Pitanga
Araçá
Gram-negative bacteria
Myrtaceae.
topic Pitanga
Araçá
Gram-negative bacteria
Myrtaceae.
description INTRODUCTION: Eugenia uniflora L. and Psidium cattleianum Sabine essential oils (EO) can be biologically active and serve as novel sources of antibiotics for Klebsiella pneumoniae and Acinetobacter baumannii bacteria.METHODS: The EO of E. uniflora L. (EOE) and P. cattleianum Sabine (EOP) were extracted from de leaves of the plants and chemically characterized by gas chromatography-mass spectrometry (GC-MS). The antioxidant potential was evaluated by the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) technique and the cytotoxicity evaluated in mammalian VERO cell line by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The antibacterial activity of EOE and EOP was assessed by the broth microdilution method.RESULTS: The major compounds of EOE were benzofuran (24.38%), germacrene B (20.12%), ?-elemene (9.33%) and ?-cubebene (8.55%), and for EOP were ?-pinene (24.25%), ?-caryophyllene (20.45%), and eucalyptol (10.43%). Both EO tested showed low antioxidant effect and dose-dependent cytotoxicity in VERO cell line. The EOE was less toxic for the mammalian cells with an IC50 (half maximal inhibitory concentration) index of 75.0 mg.mL-1. The EOP and EOE were more active against A. baumannii, with a minimum inhibitory concentration (MIC) of 14.0 and 56.0 mg.mL-1 and a minimal bactericide concentration (MBC) of 14.0 and 112.0 mg.mL-1, respectively.  CONCLUSIONS: Although EOE and EOP have low antioxidant and antibacterial activity against A. baumanni and K. pneumoniae new approaches can be applied to improve this effect in vivo.   
publishDate 2021
dc.date.none.fl_str_mv 2021-03-17
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/26521
10.34117/bjdv7n3-437
url https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/26521
identifier_str_mv 10.34117/bjdv7n3-437
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/26521/21567
dc.rights.driver.fl_str_mv Copyright (c) 2021 Brazilian Journal of Development
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Brazilian Journal of Development
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Brazilian Journals Publicações de Periódicos e Editora Ltda.
publisher.none.fl_str_mv Brazilian Journals Publicações de Periódicos e Editora Ltda.
dc.source.none.fl_str_mv Brazilian Journal of Development; Vol. 7 No. 3 (2021); 27276-27294
Brazilian Journal of Development; Vol. 7 Núm. 3 (2021); 27276-27294
Brazilian Journal of Development; v. 7 n. 3 (2021); 27276-27294
2525-8761
reponame:Revista Veras
instname:Instituto Superior de Educação Vera Cruz (VeraCruz)
instacron:VERACRUZ
instname_str Instituto Superior de Educação Vera Cruz (VeraCruz)
instacron_str VERACRUZ
institution VERACRUZ
reponame_str Revista Veras
collection Revista Veras
repository.name.fl_str_mv Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz)
repository.mail.fl_str_mv ||revistaveras@veracruz.edu.br
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