Chemical characterization, antioxidant, cytotoxic, and antibacterial activities of Eugenia uniflora L. and Psidium cattleianum Sabine essential oils against Klebsiella pneumoniae and Acinetobacter baumannii/ Caracterização química, antioxidante, citotóxica e antibacteriana dos óleos essenciais de Eugenia uniflora L. e Psidium cattleianum Sabine contra Klebsiella pneumoniae e Acinetobacter baumannii
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Veras |
Texto Completo: | https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/26521 |
Resumo: | INTRODUCTION: Eugenia uniflora L. and Psidium cattleianum Sabine essential oils (EO) can be biologically active and serve as novel sources of antibiotics for Klebsiella pneumoniae and Acinetobacter baumannii bacteria.METHODS: The EO of E. uniflora L. (EOE) and P. cattleianum Sabine (EOP) were extracted from de leaves of the plants and chemically characterized by gas chromatography-mass spectrometry (GC-MS). The antioxidant potential was evaluated by the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) technique and the cytotoxicity evaluated in mammalian VERO cell line by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The antibacterial activity of EOE and EOP was assessed by the broth microdilution method.RESULTS: The major compounds of EOE were benzofuran (24.38%), germacrene B (20.12%), ?-elemene (9.33%) and ?-cubebene (8.55%), and for EOP were ?-pinene (24.25%), ?-caryophyllene (20.45%), and eucalyptol (10.43%). Both EO tested showed low antioxidant effect and dose-dependent cytotoxicity in VERO cell line. The EOE was less toxic for the mammalian cells with an IC50 (half maximal inhibitory concentration) index of 75.0 mg.mL-1. The EOP and EOE were more active against A. baumannii, with a minimum inhibitory concentration (MIC) of 14.0 and 56.0 mg.mL-1 and a minimal bactericide concentration (MBC) of 14.0 and 112.0 mg.mL-1, respectively. CONCLUSIONS: Although EOE and EOP have low antioxidant and antibacterial activity against A. baumanni and K. pneumoniae new approaches can be applied to improve this effect in vivo. |
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Chemical characterization, antioxidant, cytotoxic, and antibacterial activities of Eugenia uniflora L. and Psidium cattleianum Sabine essential oils against Klebsiella pneumoniae and Acinetobacter baumannii/ Caracterização química, antioxidante, citotóxica e antibacteriana dos óleos essenciais de Eugenia uniflora L. e Psidium cattleianum Sabine contra Klebsiella pneumoniae e Acinetobacter baumanniiPitangaAraçáGram-negative bacteriaMyrtaceae.INTRODUCTION: Eugenia uniflora L. and Psidium cattleianum Sabine essential oils (EO) can be biologically active and serve as novel sources of antibiotics for Klebsiella pneumoniae and Acinetobacter baumannii bacteria.METHODS: The EO of E. uniflora L. (EOE) and P. cattleianum Sabine (EOP) were extracted from de leaves of the plants and chemically characterized by gas chromatography-mass spectrometry (GC-MS). The antioxidant potential was evaluated by the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) technique and the cytotoxicity evaluated in mammalian VERO cell line by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The antibacterial activity of EOE and EOP was assessed by the broth microdilution method.RESULTS: The major compounds of EOE were benzofuran (24.38%), germacrene B (20.12%), ?-elemene (9.33%) and ?-cubebene (8.55%), and for EOP were ?-pinene (24.25%), ?-caryophyllene (20.45%), and eucalyptol (10.43%). Both EO tested showed low antioxidant effect and dose-dependent cytotoxicity in VERO cell line. The EOE was less toxic for the mammalian cells with an IC50 (half maximal inhibitory concentration) index of 75.0 mg.mL-1. The EOP and EOE were more active against A. baumannii, with a minimum inhibitory concentration (MIC) of 14.0 and 56.0 mg.mL-1 and a minimal bactericide concentration (MBC) of 14.0 and 112.0 mg.mL-1, respectively. CONCLUSIONS: Although EOE and EOP have low antioxidant and antibacterial activity against A. baumanni and K. pneumoniae new approaches can be applied to improve this effect in vivo. Brazilian Journals Publicações de Periódicos e Editora Ltda.2021-03-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/2652110.34117/bjdv7n3-437Brazilian Journal of Development; Vol. 7 No. 3 (2021); 27276-27294Brazilian Journal of Development; Vol. 7 Núm. 3 (2021); 27276-27294Brazilian Journal of Development; v. 7 n. 3 (2021); 27276-272942525-8761reponame:Revista Verasinstname:Instituto Superior de Educação Vera Cruz (VeraCruz)instacron:VERACRUZenghttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/26521/21567Copyright (c) 2021 Brazilian Journal of Developmentinfo:eu-repo/semantics/openAccessGarcia, Marcelle OliveiraDa Cunha, Kamila FurtadoAllend, Suzane OlacheaDa Silva, Mirian ElertDe Santi, Ivandra IgnêsFreitag, Rogério AntonioHübner, Silvia De Oliveira RodriguesHartwig, Daiane Drawanz2022-03-29T12:15:40Zoai:ojs2.ojs.brazilianjournals.com.br:article/26521Revistahttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/PRIhttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/oai||revistaveras@veracruz.edu.br2236-57292236-5729opendoar:2024-10-15T16:14:21.829020Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz)false |
dc.title.none.fl_str_mv |
Chemical characterization, antioxidant, cytotoxic, and antibacterial activities of Eugenia uniflora L. and Psidium cattleianum Sabine essential oils against Klebsiella pneumoniae and Acinetobacter baumannii/ Caracterização química, antioxidante, citotóxica e antibacteriana dos óleos essenciais de Eugenia uniflora L. e Psidium cattleianum Sabine contra Klebsiella pneumoniae e Acinetobacter baumannii |
title |
Chemical characterization, antioxidant, cytotoxic, and antibacterial activities of Eugenia uniflora L. and Psidium cattleianum Sabine essential oils against Klebsiella pneumoniae and Acinetobacter baumannii/ Caracterização química, antioxidante, citotóxica e antibacteriana dos óleos essenciais de Eugenia uniflora L. e Psidium cattleianum Sabine contra Klebsiella pneumoniae e Acinetobacter baumannii |
spellingShingle |
Chemical characterization, antioxidant, cytotoxic, and antibacterial activities of Eugenia uniflora L. and Psidium cattleianum Sabine essential oils against Klebsiella pneumoniae and Acinetobacter baumannii/ Caracterização química, antioxidante, citotóxica e antibacteriana dos óleos essenciais de Eugenia uniflora L. e Psidium cattleianum Sabine contra Klebsiella pneumoniae e Acinetobacter baumannii Garcia, Marcelle Oliveira Pitanga Araçá Gram-negative bacteria Myrtaceae. |
title_short |
Chemical characterization, antioxidant, cytotoxic, and antibacterial activities of Eugenia uniflora L. and Psidium cattleianum Sabine essential oils against Klebsiella pneumoniae and Acinetobacter baumannii/ Caracterização química, antioxidante, citotóxica e antibacteriana dos óleos essenciais de Eugenia uniflora L. e Psidium cattleianum Sabine contra Klebsiella pneumoniae e Acinetobacter baumannii |
title_full |
Chemical characterization, antioxidant, cytotoxic, and antibacterial activities of Eugenia uniflora L. and Psidium cattleianum Sabine essential oils against Klebsiella pneumoniae and Acinetobacter baumannii/ Caracterização química, antioxidante, citotóxica e antibacteriana dos óleos essenciais de Eugenia uniflora L. e Psidium cattleianum Sabine contra Klebsiella pneumoniae e Acinetobacter baumannii |
title_fullStr |
Chemical characterization, antioxidant, cytotoxic, and antibacterial activities of Eugenia uniflora L. and Psidium cattleianum Sabine essential oils against Klebsiella pneumoniae and Acinetobacter baumannii/ Caracterização química, antioxidante, citotóxica e antibacteriana dos óleos essenciais de Eugenia uniflora L. e Psidium cattleianum Sabine contra Klebsiella pneumoniae e Acinetobacter baumannii |
title_full_unstemmed |
Chemical characterization, antioxidant, cytotoxic, and antibacterial activities of Eugenia uniflora L. and Psidium cattleianum Sabine essential oils against Klebsiella pneumoniae and Acinetobacter baumannii/ Caracterização química, antioxidante, citotóxica e antibacteriana dos óleos essenciais de Eugenia uniflora L. e Psidium cattleianum Sabine contra Klebsiella pneumoniae e Acinetobacter baumannii |
title_sort |
Chemical characterization, antioxidant, cytotoxic, and antibacterial activities of Eugenia uniflora L. and Psidium cattleianum Sabine essential oils against Klebsiella pneumoniae and Acinetobacter baumannii/ Caracterização química, antioxidante, citotóxica e antibacteriana dos óleos essenciais de Eugenia uniflora L. e Psidium cattleianum Sabine contra Klebsiella pneumoniae e Acinetobacter baumannii |
author |
Garcia, Marcelle Oliveira |
author_facet |
Garcia, Marcelle Oliveira Da Cunha, Kamila Furtado Allend, Suzane Olachea Da Silva, Mirian Elert De Santi, Ivandra Ignês Freitag, Rogério Antonio Hübner, Silvia De Oliveira Rodrigues Hartwig, Daiane Drawanz |
author_role |
author |
author2 |
Da Cunha, Kamila Furtado Allend, Suzane Olachea Da Silva, Mirian Elert De Santi, Ivandra Ignês Freitag, Rogério Antonio Hübner, Silvia De Oliveira Rodrigues Hartwig, Daiane Drawanz |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Garcia, Marcelle Oliveira Da Cunha, Kamila Furtado Allend, Suzane Olachea Da Silva, Mirian Elert De Santi, Ivandra Ignês Freitag, Rogério Antonio Hübner, Silvia De Oliveira Rodrigues Hartwig, Daiane Drawanz |
dc.subject.por.fl_str_mv |
Pitanga Araçá Gram-negative bacteria Myrtaceae. |
topic |
Pitanga Araçá Gram-negative bacteria Myrtaceae. |
description |
INTRODUCTION: Eugenia uniflora L. and Psidium cattleianum Sabine essential oils (EO) can be biologically active and serve as novel sources of antibiotics for Klebsiella pneumoniae and Acinetobacter baumannii bacteria.METHODS: The EO of E. uniflora L. (EOE) and P. cattleianum Sabine (EOP) were extracted from de leaves of the plants and chemically characterized by gas chromatography-mass spectrometry (GC-MS). The antioxidant potential was evaluated by the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) technique and the cytotoxicity evaluated in mammalian VERO cell line by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The antibacterial activity of EOE and EOP was assessed by the broth microdilution method.RESULTS: The major compounds of EOE were benzofuran (24.38%), germacrene B (20.12%), ?-elemene (9.33%) and ?-cubebene (8.55%), and for EOP were ?-pinene (24.25%), ?-caryophyllene (20.45%), and eucalyptol (10.43%). Both EO tested showed low antioxidant effect and dose-dependent cytotoxicity in VERO cell line. The EOE was less toxic for the mammalian cells with an IC50 (half maximal inhibitory concentration) index of 75.0 mg.mL-1. The EOP and EOE were more active against A. baumannii, with a minimum inhibitory concentration (MIC) of 14.0 and 56.0 mg.mL-1 and a minimal bactericide concentration (MBC) of 14.0 and 112.0 mg.mL-1, respectively. CONCLUSIONS: Although EOE and EOP have low antioxidant and antibacterial activity against A. baumanni and K. pneumoniae new approaches can be applied to improve this effect in vivo. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-03-17 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/26521 10.34117/bjdv7n3-437 |
url |
https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/26521 |
identifier_str_mv |
10.34117/bjdv7n3-437 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/26521/21567 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Brazilian Journal of Development info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Brazilian Journal of Development |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
dc.source.none.fl_str_mv |
Brazilian Journal of Development; Vol. 7 No. 3 (2021); 27276-27294 Brazilian Journal of Development; Vol. 7 Núm. 3 (2021); 27276-27294 Brazilian Journal of Development; v. 7 n. 3 (2021); 27276-27294 2525-8761 reponame:Revista Veras instname:Instituto Superior de Educação Vera Cruz (VeraCruz) instacron:VERACRUZ |
instname_str |
Instituto Superior de Educação Vera Cruz (VeraCruz) |
instacron_str |
VERACRUZ |
institution |
VERACRUZ |
reponame_str |
Revista Veras |
collection |
Revista Veras |
repository.name.fl_str_mv |
Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz) |
repository.mail.fl_str_mv |
||revistaveras@veracruz.edu.br |
_version_ |
1813645518204567552 |