Polydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin

Detalhes bibliográficos
Autor(a) principal: Rezende, Jaqueline de Paula
Data de Publicação: 2017
Outros Autores: Ferreira, Guilherme Max Dias, Ferreira, Gabriel Max Dias, Silva, Luis Henrique Mendes da, Silva, Maria do Carmo Hepanhol da, Pinto, Maximiliano Soares, Pires, Ana Clarissa dos Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1016/j.msec.2016.09.009
http://www.locus.ufv.br/handle/123456789/19238
Resumo: Bovine serum albumin (BSA) has been recognized as a marker of the cow's health, milk quality, an allergenic protein and as a carrier. Its detection is important in the food, pharmaceutical and medical industries. However, traditional techniques used to detect BSA are often time-consuming, expensive, and show limited sensitivity. This paper describes properties of polydiacetylene-triblock copolymer (L64) nanosensors, synthesized to easily detect BSA. Sensor efficiency was studied as a function of nanosensor composition, polydiacetylene chemical structures, BSA conformation and hydrophobic domain availability, using spectroscopic, calorimetric, light scattering, and electrokinetic analyses. Nanosensors were sensitive to detect the average BSA concentration of milk and dairy products and discriminated between native and denatured protein through naked-eye detectable blue-to-red transition. The standard Gibbs free energy (− 10.44 < ΔG° < − 49.52 kJ M), stoichiometry complex (1 < “n” < 3), and binding constant (6.7 × 10^2 < Ka < 4.79 × 10^8 M^− 1) of BSA-nanosensor complex formation established a direct relationship between nanosensor response and BSA-nanosensor interaction. BSA-nanosensor interaction was entropically (without cholesterol), and enthalpically driven (with cholesterol). Eugenol-BSA complex did not induce colorimetric transition. Polydiacetylene-L64 nanosensors are potential low-cost sensors for rapid detection of BSA, discriminating between native/denatured and free/bound protein.
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spelling Rezende, Jaqueline de PaulaFerreira, Guilherme Max DiasFerreira, Gabriel Max DiasSilva, Luis Henrique Mendes daSilva, Maria do Carmo Hepanhol daPinto, Maximiliano SoaresPires, Ana Clarissa dos Santos2018-05-02T13:23:24Z2018-05-02T13:23:24Z2017-01-01https://doi.org/10.1016/j.msec.2016.09.00909284931http://www.locus.ufv.br/handle/123456789/19238Bovine serum albumin (BSA) has been recognized as a marker of the cow's health, milk quality, an allergenic protein and as a carrier. Its detection is important in the food, pharmaceutical and medical industries. However, traditional techniques used to detect BSA are often time-consuming, expensive, and show limited sensitivity. This paper describes properties of polydiacetylene-triblock copolymer (L64) nanosensors, synthesized to easily detect BSA. Sensor efficiency was studied as a function of nanosensor composition, polydiacetylene chemical structures, BSA conformation and hydrophobic domain availability, using spectroscopic, calorimetric, light scattering, and electrokinetic analyses. Nanosensors were sensitive to detect the average BSA concentration of milk and dairy products and discriminated between native and denatured protein through naked-eye detectable blue-to-red transition. The standard Gibbs free energy (− 10.44 < ΔG° < − 49.52 kJ M), stoichiometry complex (1 < “n” < 3), and binding constant (6.7 × 10^2 < Ka < 4.79 × 10^8 M^− 1) of BSA-nanosensor complex formation established a direct relationship between nanosensor response and BSA-nanosensor interaction. BSA-nanosensor interaction was entropically (without cholesterol), and enthalpically driven (with cholesterol). Eugenol-BSA complex did not induce colorimetric transition. Polydiacetylene-L64 nanosensors are potential low-cost sensors for rapid detection of BSA, discriminating between native/denatured and free/bound protein.engMaterials Science and Engineering: Cv. 70, Part 1, p. 535-543, January 2017Elsevier B.Vinfo:eu-repo/semantics/openAccessNanosensorMilk proteinFluorescenceMicrocalorimetryZeta potentialPolydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf653339https://locus.ufv.br//bitstream/123456789/19238/1/artigo.pdf8b3b2d33263b179b989d22d3c67a0f66MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/19238/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg6555https://locus.ufv.br//bitstream/123456789/19238/3/artigo.pdf.jpga38e3f3d7d74bb4955683e98c16b21a5MD53123456789/192382018-05-02 23:00:40.891oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-05-03T02:00:40LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Polydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin
title Polydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin
spellingShingle Polydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin
Rezende, Jaqueline de Paula
Nanosensor
Milk protein
Fluorescence
Microcalorimetry
Zeta potential
title_short Polydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin
title_full Polydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin
title_fullStr Polydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin
title_full_unstemmed Polydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin
title_sort Polydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin
author Rezende, Jaqueline de Paula
author_facet Rezende, Jaqueline de Paula
Ferreira, Guilherme Max Dias
Ferreira, Gabriel Max Dias
Silva, Luis Henrique Mendes da
Silva, Maria do Carmo Hepanhol da
Pinto, Maximiliano Soares
Pires, Ana Clarissa dos Santos
author_role author
author2 Ferreira, Guilherme Max Dias
Ferreira, Gabriel Max Dias
Silva, Luis Henrique Mendes da
Silva, Maria do Carmo Hepanhol da
Pinto, Maximiliano Soares
Pires, Ana Clarissa dos Santos
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rezende, Jaqueline de Paula
Ferreira, Guilherme Max Dias
Ferreira, Gabriel Max Dias
Silva, Luis Henrique Mendes da
Silva, Maria do Carmo Hepanhol da
Pinto, Maximiliano Soares
Pires, Ana Clarissa dos Santos
dc.subject.pt-BR.fl_str_mv Nanosensor
Milk protein
Fluorescence
Microcalorimetry
Zeta potential
topic Nanosensor
Milk protein
Fluorescence
Microcalorimetry
Zeta potential
description Bovine serum albumin (BSA) has been recognized as a marker of the cow's health, milk quality, an allergenic protein and as a carrier. Its detection is important in the food, pharmaceutical and medical industries. However, traditional techniques used to detect BSA are often time-consuming, expensive, and show limited sensitivity. This paper describes properties of polydiacetylene-triblock copolymer (L64) nanosensors, synthesized to easily detect BSA. Sensor efficiency was studied as a function of nanosensor composition, polydiacetylene chemical structures, BSA conformation and hydrophobic domain availability, using spectroscopic, calorimetric, light scattering, and electrokinetic analyses. Nanosensors were sensitive to detect the average BSA concentration of milk and dairy products and discriminated between native and denatured protein through naked-eye detectable blue-to-red transition. The standard Gibbs free energy (− 10.44 < ΔG° < − 49.52 kJ M), stoichiometry complex (1 < “n” < 3), and binding constant (6.7 × 10^2 < Ka < 4.79 × 10^8 M^− 1) of BSA-nanosensor complex formation established a direct relationship between nanosensor response and BSA-nanosensor interaction. BSA-nanosensor interaction was entropically (without cholesterol), and enthalpically driven (with cholesterol). Eugenol-BSA complex did not induce colorimetric transition. Polydiacetylene-L64 nanosensors are potential low-cost sensors for rapid detection of BSA, discriminating between native/denatured and free/bound protein.
publishDate 2017
dc.date.issued.fl_str_mv 2017-01-01
dc.date.accessioned.fl_str_mv 2018-05-02T13:23:24Z
dc.date.available.fl_str_mv 2018-05-02T13:23:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv https://doi.org/10.1016/j.msec.2016.09.009
09284931
http://www.locus.ufv.br/handle/123456789/19238
url https://doi.org/10.1016/j.msec.2016.09.009
http://www.locus.ufv.br/handle/123456789/19238
identifier_str_mv 09284931
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv v. 70, Part 1, p. 535-543, January 2017
dc.rights.driver.fl_str_mv Elsevier B.V
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