Deformation of Mitochondrial Cristae in Human Neural Progenitor Cells Exposed to Valproic Acid

Detalhes bibliográficos
Autor(a) principal: COSTA,RODRIGO M. DA
Data de Publicação: 2018
Outros Autores: KARMIRIAN,KARINA, REHEN,STEVENS K.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502223
Resumo: ABSTRACT Neural development represents a dynamic process where mitochondrial integrity is decisive for neuronal activity. Structural changes in these organelles may be related to neurological disorders. Valproic acid (VPA) is an anticonvulsive drug commonly used for epilepsy treatment and its use is associated to increased risk of neuropsychiatric disorders. Recently we showed changes in shape and membrane potential in mitochondria from human neural progenitor cells (NPCs) exposed to VPA (da Costa et al. 2015). Here, we applied transmission electron microscopy and electron tomography to evaluate mitochondrial damage caused by VPA in NPCs. Results showed mitochondrial cristae disorganization in a dose dependent manner. Disturbance in mitochondrial ultrastructure may influence metabolism, leading to synaptic plasticity and neurogenesis impairment. These data contribute to understanding VPA exposure potential effects on brain development.
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spelling Deformation of Mitochondrial Cristae in Human Neural Progenitor Cells Exposed to Valproic Acidtransmission electron microscopymitochondriavalproic acidepilepsyhuman neural progenitor cellselectron tomographyABSTRACT Neural development represents a dynamic process where mitochondrial integrity is decisive for neuronal activity. Structural changes in these organelles may be related to neurological disorders. Valproic acid (VPA) is an anticonvulsive drug commonly used for epilepsy treatment and its use is associated to increased risk of neuropsychiatric disorders. Recently we showed changes in shape and membrane potential in mitochondria from human neural progenitor cells (NPCs) exposed to VPA (da Costa et al. 2015). Here, we applied transmission electron microscopy and electron tomography to evaluate mitochondrial damage caused by VPA in NPCs. Results showed mitochondrial cristae disorganization in a dose dependent manner. Disturbance in mitochondrial ultrastructure may influence metabolism, leading to synaptic plasticity and neurogenesis impairment. These data contribute to understanding VPA exposure potential effects on brain development.Academia Brasileira de Ciências2018-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502223Anais da Academia Brasileira de Ciências v.90 n.2 suppl.1 2018reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201820170762info:eu-repo/semantics/openAccessCOSTA,RODRIGO M. DAKARMIRIAN,KARINAREHEN,STEVENS K.eng2019-11-29T00:00:00Zoai:scielo:S0001-37652018000502223Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2019-11-29T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Deformation of Mitochondrial Cristae in Human Neural Progenitor Cells Exposed to Valproic Acid
title Deformation of Mitochondrial Cristae in Human Neural Progenitor Cells Exposed to Valproic Acid
spellingShingle Deformation of Mitochondrial Cristae in Human Neural Progenitor Cells Exposed to Valproic Acid
COSTA,RODRIGO M. DA
transmission electron microscopy
mitochondria
valproic acid
epilepsy
human neural progenitor cells
electron tomography
title_short Deformation of Mitochondrial Cristae in Human Neural Progenitor Cells Exposed to Valproic Acid
title_full Deformation of Mitochondrial Cristae in Human Neural Progenitor Cells Exposed to Valproic Acid
title_fullStr Deformation of Mitochondrial Cristae in Human Neural Progenitor Cells Exposed to Valproic Acid
title_full_unstemmed Deformation of Mitochondrial Cristae in Human Neural Progenitor Cells Exposed to Valproic Acid
title_sort Deformation of Mitochondrial Cristae in Human Neural Progenitor Cells Exposed to Valproic Acid
author COSTA,RODRIGO M. DA
author_facet COSTA,RODRIGO M. DA
KARMIRIAN,KARINA
REHEN,STEVENS K.
author_role author
author2 KARMIRIAN,KARINA
REHEN,STEVENS K.
author2_role author
author
dc.contributor.author.fl_str_mv COSTA,RODRIGO M. DA
KARMIRIAN,KARINA
REHEN,STEVENS K.
dc.subject.por.fl_str_mv transmission electron microscopy
mitochondria
valproic acid
epilepsy
human neural progenitor cells
electron tomography
topic transmission electron microscopy
mitochondria
valproic acid
epilepsy
human neural progenitor cells
electron tomography
description ABSTRACT Neural development represents a dynamic process where mitochondrial integrity is decisive for neuronal activity. Structural changes in these organelles may be related to neurological disorders. Valproic acid (VPA) is an anticonvulsive drug commonly used for epilepsy treatment and its use is associated to increased risk of neuropsychiatric disorders. Recently we showed changes in shape and membrane potential in mitochondria from human neural progenitor cells (NPCs) exposed to VPA (da Costa et al. 2015). Here, we applied transmission electron microscopy and electron tomography to evaluate mitochondrial damage caused by VPA in NPCs. Results showed mitochondrial cristae disorganization in a dose dependent manner. Disturbance in mitochondrial ultrastructure may influence metabolism, leading to synaptic plasticity and neurogenesis impairment. These data contribute to understanding VPA exposure potential effects on brain development.
publishDate 2018
dc.date.none.fl_str_mv 2018-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502223
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502223
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765201820170762
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.90 n.2 suppl.1 2018
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
instacron:ABC
instname_str Academia Brasileira de Ciências (ABC)
instacron_str ABC
institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
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