Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900606 |
Resumo: | DNA repair pathways, cell cycle checkpoints, and redox protection systems are essential factors for securing genomic stability. The aim of the present study was to analyze the effect of Ilex paraguariensis (Ip) infusion and one of its polyphenolic components rutin on cellular and molecular damage induced by ionizing radiation. Ip is a beverage drank by most inhabitants of Argentina, Paraguay, Southern Brazil, and Uruguay. The yeast Saccharomyces cerevisiae (SC7Klys 2-3) was used as the eukaryotic model. Exponentially growing cells were exposed to gamma rays (γ) in the presence or absence of Ip or rutin. The concentrations used simulated those found in the habitual infusion. Surviving fractions, mutation frequency, and DNA double-strand breaks (DSB) were determined after treatments. A significant increase in surviving fractions after gamma irradiation was observed following combined exposure to γ+R, or γ+Ip. Upon these concomitant treatments, mutation and DSB frequency decreased significantly. In the mutant strain deficient in MEC1, a significant increase in γ sensitivity and a low effect of rutin on γ-induced chromosomal fragmentation was observed. Results were interpreted in the framework of a model of interaction between radiation-induced free radicals, DNA repair pathways, and checkpoint controls, where the DNA damage that induced activation of MEC1 nodal point of the network could be modulated by Ip components including rutin. Furthermore, ionizing radiation-induced redox cascades can be interrupted by rutin potential and other protectors contained in Ip. |
id |
ABDC-1_dcf9ce1cd8c935ccd12480f6ec440e52 |
---|---|
oai_identifier_str |
oai:scielo:S0100-879X2018000900606 |
network_acronym_str |
ABDC-1 |
network_name_str |
Brazilian Journal of Medical and Biological Research |
repository_id_str |
|
spelling |
Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutinRadiation damageIlex paraguariensis infusionRutinRadioprotectionAntimutagenic effectsSaccharomyces cerevisiaeDNA repair pathways, cell cycle checkpoints, and redox protection systems are essential factors for securing genomic stability. The aim of the present study was to analyze the effect of Ilex paraguariensis (Ip) infusion and one of its polyphenolic components rutin on cellular and molecular damage induced by ionizing radiation. Ip is a beverage drank by most inhabitants of Argentina, Paraguay, Southern Brazil, and Uruguay. The yeast Saccharomyces cerevisiae (SC7Klys 2-3) was used as the eukaryotic model. Exponentially growing cells were exposed to gamma rays (γ) in the presence or absence of Ip or rutin. The concentrations used simulated those found in the habitual infusion. Surviving fractions, mutation frequency, and DNA double-strand breaks (DSB) were determined after treatments. A significant increase in surviving fractions after gamma irradiation was observed following combined exposure to γ+R, or γ+Ip. Upon these concomitant treatments, mutation and DSB frequency decreased significantly. In the mutant strain deficient in MEC1, a significant increase in γ sensitivity and a low effect of rutin on γ-induced chromosomal fragmentation was observed. Results were interpreted in the framework of a model of interaction between radiation-induced free radicals, DNA repair pathways, and checkpoint controls, where the DNA damage that induced activation of MEC1 nodal point of the network could be modulated by Ip components including rutin. Furthermore, ionizing radiation-induced redox cascades can be interrupted by rutin potential and other protectors contained in Ip.Associação Brasileira de Divulgação Científica2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900606Brazilian Journal of Medical and Biological Research v.51 n.9 2018reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/1414-431x20187404info:eu-repo/semantics/openAccessBracesco,N.Sosa,V.Blanc,L.Contreras,V.Candreva,E.C.Salvo,V.A.Hocart,S.Mechoso,B.Nunes,E.eng2019-03-19T00:00:00Zoai:scielo:S0100-879X2018000900606Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2019-03-19T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin |
title |
Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin |
spellingShingle |
Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin Bracesco,N. Radiation damage Ilex paraguariensis infusion Rutin Radioprotection Antimutagenic effects Saccharomyces cerevisiae |
title_short |
Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin |
title_full |
Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin |
title_fullStr |
Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin |
title_full_unstemmed |
Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin |
title_sort |
Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin |
author |
Bracesco,N. |
author_facet |
Bracesco,N. Sosa,V. Blanc,L. Contreras,V. Candreva,E.C. Salvo,V.A. Hocart,S. Mechoso,B. Nunes,E. |
author_role |
author |
author2 |
Sosa,V. Blanc,L. Contreras,V. Candreva,E.C. Salvo,V.A. Hocart,S. Mechoso,B. Nunes,E. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Bracesco,N. Sosa,V. Blanc,L. Contreras,V. Candreva,E.C. Salvo,V.A. Hocart,S. Mechoso,B. Nunes,E. |
dc.subject.por.fl_str_mv |
Radiation damage Ilex paraguariensis infusion Rutin Radioprotection Antimutagenic effects Saccharomyces cerevisiae |
topic |
Radiation damage Ilex paraguariensis infusion Rutin Radioprotection Antimutagenic effects Saccharomyces cerevisiae |
description |
DNA repair pathways, cell cycle checkpoints, and redox protection systems are essential factors for securing genomic stability. The aim of the present study was to analyze the effect of Ilex paraguariensis (Ip) infusion and one of its polyphenolic components rutin on cellular and molecular damage induced by ionizing radiation. Ip is a beverage drank by most inhabitants of Argentina, Paraguay, Southern Brazil, and Uruguay. The yeast Saccharomyces cerevisiae (SC7Klys 2-3) was used as the eukaryotic model. Exponentially growing cells were exposed to gamma rays (γ) in the presence or absence of Ip or rutin. The concentrations used simulated those found in the habitual infusion. Surviving fractions, mutation frequency, and DNA double-strand breaks (DSB) were determined after treatments. A significant increase in surviving fractions after gamma irradiation was observed following combined exposure to γ+R, or γ+Ip. Upon these concomitant treatments, mutation and DSB frequency decreased significantly. In the mutant strain deficient in MEC1, a significant increase in γ sensitivity and a low effect of rutin on γ-induced chromosomal fragmentation was observed. Results were interpreted in the framework of a model of interaction between radiation-induced free radicals, DNA repair pathways, and checkpoint controls, where the DNA damage that induced activation of MEC1 nodal point of the network could be modulated by Ip components including rutin. Furthermore, ionizing radiation-induced redox cascades can be interrupted by rutin potential and other protectors contained in Ip. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900606 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900606 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1414-431x20187404 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.51 n.9 2018 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302946660581376 |