A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media

Detalhes bibliográficos
Autor(a) principal: Schneedorf,J.M.
Data de Publicação: 2001
Outros Autores: Santoro,M.M., Lovrien,R.E., Mares-Guia,M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800012
Resumo: Synthetic dyes bind to proteins causing selective coprecipitation of the complexes in acid aqueous solution by a process of reversible denaturation that can be used as an alternative method for protein fractionation. The events that occur before precipitation were investigated by equilibrium dialysis using bovine trypsin and flavianic acid as a model able to cause coprecipitation. A two-step mode of interaction was found to be dependent on the incubation periods allowed for binding, with pronounced binding occurring after 42 h of incubation. The first step seems to involve hydration effects and conformational changes induced by binding of the first dye molecule, following rapid denaturation due to the binding of six additional flavianate anions to the macromolecule.
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spelling A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid mediadye bindingbinding isothermcoprecipitationSynthetic dyes bind to proteins causing selective coprecipitation of the complexes in acid aqueous solution by a process of reversible denaturation that can be used as an alternative method for protein fractionation. The events that occur before precipitation were investigated by equilibrium dialysis using bovine trypsin and flavianic acid as a model able to cause coprecipitation. A two-step mode of interaction was found to be dependent on the incubation periods allowed for binding, with pronounced binding occurring after 42 h of incubation. The first step seems to involve hydration effects and conformational changes induced by binding of the first dye molecule, following rapid denaturation due to the binding of six additional flavianate anions to the macromolecule.Associação Brasileira de Divulgação Científica2001-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800012Brazilian Journal of Medical and Biological Research v.34 n.8 2001reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2001000800012info:eu-repo/semantics/openAccessSchneedorf,J.M.Santoro,M.M.Lovrien,R.E.Mares-Guia,M.eng2001-07-20T00:00:00Zoai:scielo:S0100-879X2001000800012Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2001-07-20T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media
title A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media
spellingShingle A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media
Schneedorf,J.M.
dye binding
binding isotherm
coprecipitation
title_short A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media
title_full A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media
title_fullStr A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media
title_full_unstemmed A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media
title_sort A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media
author Schneedorf,J.M.
author_facet Schneedorf,J.M.
Santoro,M.M.
Lovrien,R.E.
Mares-Guia,M.
author_role author
author2 Santoro,M.M.
Lovrien,R.E.
Mares-Guia,M.
author2_role author
author
author
dc.contributor.author.fl_str_mv Schneedorf,J.M.
Santoro,M.M.
Lovrien,R.E.
Mares-Guia,M.
dc.subject.por.fl_str_mv dye binding
binding isotherm
coprecipitation
topic dye binding
binding isotherm
coprecipitation
description Synthetic dyes bind to proteins causing selective coprecipitation of the complexes in acid aqueous solution by a process of reversible denaturation that can be used as an alternative method for protein fractionation. The events that occur before precipitation were investigated by equilibrium dialysis using bovine trypsin and flavianic acid as a model able to cause coprecipitation. A two-step mode of interaction was found to be dependent on the incubation periods allowed for binding, with pronounced binding occurring after 42 h of incubation. The first step seems to involve hydration effects and conformational changes induced by binding of the first dye molecule, following rapid denaturation due to the binding of six additional flavianate anions to the macromolecule.
publishDate 2001
dc.date.none.fl_str_mv 2001-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X2001000800012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.34 n.8 2001
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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