Spectrophotometric determination of total proteins in blood plasma: a comparative study among dye-binding methods

Detalhes bibliográficos
Autor(a) principal: Zaia,Dimas Augusto Morozin
Data de Publicação: 2005
Outros Autores: Marques,Fábio Rangel, Zaia,Cássia Thaïs Bussamra Vieira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300008
Resumo: A comparative study between the biuret method (standard method for total proteins) and spectrophotometric methods using dyes (Bradford, 3',3",5',5"-tetrabromophenolphthalein ethyl ester-TBPEE, and erythrosin-B) was carried out for the determination of total proteins in blood plasma from rats. Bradford method showed the highest sensitivity for proteins and biuret method showed the lowest. For all the methods, the absorbance for different proteins (BSA, casein, and egg albumin) was measured and Bradford method showed the lowest variation of absorbance. The concentration of total protein obtained by using Bradford method was not statistically different (p>0.05) from concentration of total protein obtained by the biuret method. But in regard to erythrosin-B and TBPEE methods the concentrations of total protein were statistically different (p<0.05). Thus, Bradford method could be used instead of the biuret method for determination of total proteins in blood plasma.
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spelling Spectrophotometric determination of total proteins in blood plasma: a comparative study among dye-binding methodsTotal proteinsspectrophotometryblood plasmadye-binding methodsA comparative study between the biuret method (standard method for total proteins) and spectrophotometric methods using dyes (Bradford, 3',3",5',5"-tetrabromophenolphthalein ethyl ester-TBPEE, and erythrosin-B) was carried out for the determination of total proteins in blood plasma from rats. Bradford method showed the highest sensitivity for proteins and biuret method showed the lowest. For all the methods, the absorbance for different proteins (BSA, casein, and egg albumin) was measured and Bradford method showed the lowest variation of absorbance. The concentration of total protein obtained by using Bradford method was not statistically different (p>0.05) from concentration of total protein obtained by the biuret method. But in regard to erythrosin-B and TBPEE methods the concentrations of total protein were statistically different (p<0.05). Thus, Bradford method could be used instead of the biuret method for determination of total proteins in blood plasma.Instituto de Tecnologia do Paraná - Tecpar2005-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300008Brazilian Archives of Biology and Technology v.48 n.3 2005reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132005000300008info:eu-repo/semantics/openAccessZaia,Dimas Augusto MorozinMarques,Fábio RangelZaia,Cássia Thaïs Bussamra Vieiraeng2005-08-03T00:00:00Zoai:scielo:S1516-89132005000300008Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2005-08-03T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Spectrophotometric determination of total proteins in blood plasma: a comparative study among dye-binding methods
title Spectrophotometric determination of total proteins in blood plasma: a comparative study among dye-binding methods
spellingShingle Spectrophotometric determination of total proteins in blood plasma: a comparative study among dye-binding methods
Zaia,Dimas Augusto Morozin
Total proteins
spectrophotometry
blood plasma
dye-binding methods
title_short Spectrophotometric determination of total proteins in blood plasma: a comparative study among dye-binding methods
title_full Spectrophotometric determination of total proteins in blood plasma: a comparative study among dye-binding methods
title_fullStr Spectrophotometric determination of total proteins in blood plasma: a comparative study among dye-binding methods
title_full_unstemmed Spectrophotometric determination of total proteins in blood plasma: a comparative study among dye-binding methods
title_sort Spectrophotometric determination of total proteins in blood plasma: a comparative study among dye-binding methods
author Zaia,Dimas Augusto Morozin
author_facet Zaia,Dimas Augusto Morozin
Marques,Fábio Rangel
Zaia,Cássia Thaïs Bussamra Vieira
author_role author
author2 Marques,Fábio Rangel
Zaia,Cássia Thaïs Bussamra Vieira
author2_role author
author
dc.contributor.author.fl_str_mv Zaia,Dimas Augusto Morozin
Marques,Fábio Rangel
Zaia,Cássia Thaïs Bussamra Vieira
dc.subject.por.fl_str_mv Total proteins
spectrophotometry
blood plasma
dye-binding methods
topic Total proteins
spectrophotometry
blood plasma
dye-binding methods
description A comparative study between the biuret method (standard method for total proteins) and spectrophotometric methods using dyes (Bradford, 3',3",5',5"-tetrabromophenolphthalein ethyl ester-TBPEE, and erythrosin-B) was carried out for the determination of total proteins in blood plasma from rats. Bradford method showed the highest sensitivity for proteins and biuret method showed the lowest. For all the methods, the absorbance for different proteins (BSA, casein, and egg albumin) was measured and Bradford method showed the lowest variation of absorbance. The concentration of total protein obtained by using Bradford method was not statistically different (p>0.05) from concentration of total protein obtained by the biuret method. But in regard to erythrosin-B and TBPEE methods the concentrations of total protein were statistically different (p<0.05). Thus, Bradford method could be used instead of the biuret method for determination of total proteins in blood plasma.
publishDate 2005
dc.date.none.fl_str_mv 2005-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132005000300008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.48 n.3 2005
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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