Assessing the diversity of the virulence potential ofEscherichia coli isolated from bacteremia in São Paulo, Brazil

Detalhes bibliográficos
Autor(a) principal: Santos,A.C.M.
Data de Publicação: 2013
Outros Autores: Zidko,A.C.M., Pignatari,A.C., Silva,R.M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100968
Resumo: Most of the knowledge of the virulence determinants of extraintestinal pathogenicEscherichia coli (ExPEC) comes from studies with human strains causing urinary tract infections and neonatal meningitis and animal strains causing avian colibacillosis. In this research, we analyzed the phylogenetic background, the presence of 20 ExPEC virulence factors, and the intrinsic virulence potential of 74 E. coli strains isolated in São Paulo, Brazil, from 74 hospitalized patients (43 males and 31 females) with unknown-source bacteremia. Unlike other places in the world, the bacteremic strains originated equally from phylogroups B2 (35%) and D (30%). A great variability in the profiles of virulence factors was noted in this survey. Nevertheless, 61% of the strains were classified as ExPEC, meaning that they possessed intrinsic virulent potential. Accordingly, these strains presented high virulence factor scores (average of 8.7), and were positively associated with 12 of 17 virulence factors detected. On the contrary, the non-ExPEC strains, isolated from 39% of the patients, presented a generally low virulence capacity (medium virulence factor score of 3.1), and were positively associated with only the colicin cvaC gene. These results show the importance of discriminating E. coli isolates that possess characteristics of true pathogens from those that may be merely opportunistic in order to better understand the virulence mechanisms involved in extraintestinalE. coli infections. Such knowledge is essential for epidemiological purposes as well as for development of control measures aimed to minimize the incidence of these life-threatening and costly infections.
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spelling Assessing the diversity of the virulence potential ofEscherichia coli isolated from bacteremia in São Paulo, BrazilBacteremiaExtraintestinal pathogenic Escherichia coliVirulence factorsIntrinsic virulence potentialPhylogenetic backgroundMost of the knowledge of the virulence determinants of extraintestinal pathogenicEscherichia coli (ExPEC) comes from studies with human strains causing urinary tract infections and neonatal meningitis and animal strains causing avian colibacillosis. In this research, we analyzed the phylogenetic background, the presence of 20 ExPEC virulence factors, and the intrinsic virulence potential of 74 E. coli strains isolated in São Paulo, Brazil, from 74 hospitalized patients (43 males and 31 females) with unknown-source bacteremia. Unlike other places in the world, the bacteremic strains originated equally from phylogroups B2 (35%) and D (30%). A great variability in the profiles of virulence factors was noted in this survey. Nevertheless, 61% of the strains were classified as ExPEC, meaning that they possessed intrinsic virulent potential. Accordingly, these strains presented high virulence factor scores (average of 8.7), and were positively associated with 12 of 17 virulence factors detected. On the contrary, the non-ExPEC strains, isolated from 39% of the patients, presented a generally low virulence capacity (medium virulence factor score of 3.1), and were positively associated with only the colicin cvaC gene. These results show the importance of discriminating E. coli isolates that possess characteristics of true pathogens from those that may be merely opportunistic in order to better understand the virulence mechanisms involved in extraintestinalE. coli infections. Such knowledge is essential for epidemiological purposes as well as for development of control measures aimed to minimize the incidence of these life-threatening and costly infections.Associação Brasileira de Divulgação Científica2013-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100968Brazilian Journal of Medical and Biological Research v.46 n.11 2013reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/1414-431X20133184info:eu-repo/semantics/openAccessSantos,A.C.M.Zidko,A.C.M.Pignatari,A.C.Silva,R.M.eng2015-10-08T00:00:00Zoai:scielo:S0100-879X2013001100968Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2015-10-08T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Assessing the diversity of the virulence potential ofEscherichia coli isolated from bacteremia in São Paulo, Brazil
title Assessing the diversity of the virulence potential ofEscherichia coli isolated from bacteremia in São Paulo, Brazil
spellingShingle Assessing the diversity of the virulence potential ofEscherichia coli isolated from bacteremia in São Paulo, Brazil
Santos,A.C.M.
Bacteremia
Extraintestinal pathogenic Escherichia coli
Virulence factors
Intrinsic virulence potential
Phylogenetic background
title_short Assessing the diversity of the virulence potential ofEscherichia coli isolated from bacteremia in São Paulo, Brazil
title_full Assessing the diversity of the virulence potential ofEscherichia coli isolated from bacteremia in São Paulo, Brazil
title_fullStr Assessing the diversity of the virulence potential ofEscherichia coli isolated from bacteremia in São Paulo, Brazil
title_full_unstemmed Assessing the diversity of the virulence potential ofEscherichia coli isolated from bacteremia in São Paulo, Brazil
title_sort Assessing the diversity of the virulence potential ofEscherichia coli isolated from bacteremia in São Paulo, Brazil
author Santos,A.C.M.
author_facet Santos,A.C.M.
Zidko,A.C.M.
Pignatari,A.C.
Silva,R.M.
author_role author
author2 Zidko,A.C.M.
Pignatari,A.C.
Silva,R.M.
author2_role author
author
author
dc.contributor.author.fl_str_mv Santos,A.C.M.
Zidko,A.C.M.
Pignatari,A.C.
Silva,R.M.
dc.subject.por.fl_str_mv Bacteremia
Extraintestinal pathogenic Escherichia coli
Virulence factors
Intrinsic virulence potential
Phylogenetic background
topic Bacteremia
Extraintestinal pathogenic Escherichia coli
Virulence factors
Intrinsic virulence potential
Phylogenetic background
description Most of the knowledge of the virulence determinants of extraintestinal pathogenicEscherichia coli (ExPEC) comes from studies with human strains causing urinary tract infections and neonatal meningitis and animal strains causing avian colibacillosis. In this research, we analyzed the phylogenetic background, the presence of 20 ExPEC virulence factors, and the intrinsic virulence potential of 74 E. coli strains isolated in São Paulo, Brazil, from 74 hospitalized patients (43 males and 31 females) with unknown-source bacteremia. Unlike other places in the world, the bacteremic strains originated equally from phylogroups B2 (35%) and D (30%). A great variability in the profiles of virulence factors was noted in this survey. Nevertheless, 61% of the strains were classified as ExPEC, meaning that they possessed intrinsic virulent potential. Accordingly, these strains presented high virulence factor scores (average of 8.7), and were positively associated with 12 of 17 virulence factors detected. On the contrary, the non-ExPEC strains, isolated from 39% of the patients, presented a generally low virulence capacity (medium virulence factor score of 3.1), and were positively associated with only the colicin cvaC gene. These results show the importance of discriminating E. coli isolates that possess characteristics of true pathogens from those that may be merely opportunistic in order to better understand the virulence mechanisms involved in extraintestinalE. coli infections. Such knowledge is essential for epidemiological purposes as well as for development of control measures aimed to minimize the incidence of these life-threatening and costly infections.
publishDate 2013
dc.date.none.fl_str_mv 2013-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100968
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1414-431X20133184
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.46 n.11 2013
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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