EFFECTS OF DIFFUSION ON THE KINETICS OF MALTOSE HYDROLYSIS USING GLUCOAMYLASE IMMOBILIZED ON MACROPOROUS SILICA
Autor(a) principal: | |
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Data de Publicação: | 1997 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321997000400007 |
Resumo: | In this work the effects of diffusion on the hydrolysis of maltose using glucoamylase immobilized on macroporous silica at 30o C are studied. Two different kinds of experimental assays are carried out: one using free enzyme and the other using an enzyme covalently linked to macroporous silica, preactivated with glutaraldehyde. Free enzyme assays are used to estimate the kinetic parameters for hydrolysis of maltose at 30ºC, according to a Michaelis-Menten equation, with inhibition by the product. Runs with the immobilized enzyme enable us to estimate dextrin diffusivities in silica, assuming that the inherent kinetic parameters are equal to the intrinsic ones. The values of kinetic and mass transfer parameters are: K3 = 0.90x10-5 g/U.s, Km = 0.61x10-3 g/ml, Ki = 1.23x10-1 g/ml, DMs = 0.50x10-6 cm2 /s and DGs = 0.55x10-6 cm2 /s |
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Brazilian Journal of Chemical Engineering |
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EFFECTS OF DIFFUSION ON THE KINETICS OF MALTOSE HYDROLYSIS USING GLUCOAMYLASE IMMOBILIZED ON MACROPOROUS SILICAMaltose hydrolysisdextrin diffusivitykinetic parametersIn this work the effects of diffusion on the hydrolysis of maltose using glucoamylase immobilized on macroporous silica at 30o C are studied. Two different kinds of experimental assays are carried out: one using free enzyme and the other using an enzyme covalently linked to macroporous silica, preactivated with glutaraldehyde. Free enzyme assays are used to estimate the kinetic parameters for hydrolysis of maltose at 30ºC, according to a Michaelis-Menten equation, with inhibition by the product. Runs with the immobilized enzyme enable us to estimate dextrin diffusivities in silica, assuming that the inherent kinetic parameters are equal to the intrinsic ones. The values of kinetic and mass transfer parameters are: K3 = 0.90x10-5 g/U.s, Km = 0.61x10-3 g/ml, Ki = 1.23x10-1 g/ml, DMs = 0.50x10-6 cm2 /s and DGs = 0.55x10-6 cm2 /sBrazilian Society of Chemical Engineering1997-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321997000400007Brazilian Journal of Chemical Engineering v.14 n.4 1997reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66321997000400007info:eu-repo/semantics/openAccessGonçalves,L.R.B.Giordano,R.L.C.Giordano,R.C.eng1998-10-06T00:00:00Zoai:scielo:S0104-66321997000400007Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:1998-10-06T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
EFFECTS OF DIFFUSION ON THE KINETICS OF MALTOSE HYDROLYSIS USING GLUCOAMYLASE IMMOBILIZED ON MACROPOROUS SILICA |
title |
EFFECTS OF DIFFUSION ON THE KINETICS OF MALTOSE HYDROLYSIS USING GLUCOAMYLASE IMMOBILIZED ON MACROPOROUS SILICA |
spellingShingle |
EFFECTS OF DIFFUSION ON THE KINETICS OF MALTOSE HYDROLYSIS USING GLUCOAMYLASE IMMOBILIZED ON MACROPOROUS SILICA Gonçalves,L.R.B. Maltose hydrolysis dextrin diffusivity kinetic parameters |
title_short |
EFFECTS OF DIFFUSION ON THE KINETICS OF MALTOSE HYDROLYSIS USING GLUCOAMYLASE IMMOBILIZED ON MACROPOROUS SILICA |
title_full |
EFFECTS OF DIFFUSION ON THE KINETICS OF MALTOSE HYDROLYSIS USING GLUCOAMYLASE IMMOBILIZED ON MACROPOROUS SILICA |
title_fullStr |
EFFECTS OF DIFFUSION ON THE KINETICS OF MALTOSE HYDROLYSIS USING GLUCOAMYLASE IMMOBILIZED ON MACROPOROUS SILICA |
title_full_unstemmed |
EFFECTS OF DIFFUSION ON THE KINETICS OF MALTOSE HYDROLYSIS USING GLUCOAMYLASE IMMOBILIZED ON MACROPOROUS SILICA |
title_sort |
EFFECTS OF DIFFUSION ON THE KINETICS OF MALTOSE HYDROLYSIS USING GLUCOAMYLASE IMMOBILIZED ON MACROPOROUS SILICA |
author |
Gonçalves,L.R.B. |
author_facet |
Gonçalves,L.R.B. Giordano,R.L.C. Giordano,R.C. |
author_role |
author |
author2 |
Giordano,R.L.C. Giordano,R.C. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Gonçalves,L.R.B. Giordano,R.L.C. Giordano,R.C. |
dc.subject.por.fl_str_mv |
Maltose hydrolysis dextrin diffusivity kinetic parameters |
topic |
Maltose hydrolysis dextrin diffusivity kinetic parameters |
description |
In this work the effects of diffusion on the hydrolysis of maltose using glucoamylase immobilized on macroporous silica at 30o C are studied. Two different kinds of experimental assays are carried out: one using free enzyme and the other using an enzyme covalently linked to macroporous silica, preactivated with glutaraldehyde. Free enzyme assays are used to estimate the kinetic parameters for hydrolysis of maltose at 30ºC, according to a Michaelis-Menten equation, with inhibition by the product. Runs with the immobilized enzyme enable us to estimate dextrin diffusivities in silica, assuming that the inherent kinetic parameters are equal to the intrinsic ones. The values of kinetic and mass transfer parameters are: K3 = 0.90x10-5 g/U.s, Km = 0.61x10-3 g/ml, Ki = 1.23x10-1 g/ml, DMs = 0.50x10-6 cm2 /s and DGs = 0.55x10-6 cm2 /s |
publishDate |
1997 |
dc.date.none.fl_str_mv |
1997-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321997000400007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321997000400007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0104-66321997000400007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.14 n.4 1997 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
_version_ |
1754213170309758976 |