EFFECTS OF DIFFUSION ON THE KINETICS OF MALTOSE HYDROLYSIS USING GLUCOAMYLASE IMMOBILIZED ON MACROPOROUS SILICA

Detalhes bibliográficos
Autor(a) principal: Gonçalves,L.R.B.
Data de Publicação: 1997
Outros Autores: Giordano,R.L.C., Giordano,R.C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Chemical Engineering
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321997000400007
Resumo: In this work the effects of diffusion on the hydrolysis of maltose using glucoamylase immobilized on macroporous silica at 30o C are studied. Two different kinds of experimental assays are carried out: one using free enzyme and the other using an enzyme covalently linked to macroporous silica, preactivated with glutaraldehyde. Free enzyme assays are used to estimate the kinetic parameters for hydrolysis of maltose at 30ºC, according to a Michaelis-Menten equation, with inhibition by the product. Runs with the immobilized enzyme enable us to estimate dextrin diffusivities in silica, assuming that the inherent kinetic parameters are equal to the intrinsic ones. The values of kinetic and mass transfer parameters are: K3 = 0.90x10-5 g/U.s, Km = 0.61x10-3 g/ml, Ki = 1.23x10-1 g/ml, DMs = 0.50x10-6 cm2 /s and DGs = 0.55x10-6 cm2 /s
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spelling EFFECTS OF DIFFUSION ON THE KINETICS OF MALTOSE HYDROLYSIS USING GLUCOAMYLASE IMMOBILIZED ON MACROPOROUS SILICAMaltose hydrolysisdextrin diffusivitykinetic parametersIn this work the effects of diffusion on the hydrolysis of maltose using glucoamylase immobilized on macroporous silica at 30o C are studied. Two different kinds of experimental assays are carried out: one using free enzyme and the other using an enzyme covalently linked to macroporous silica, preactivated with glutaraldehyde. Free enzyme assays are used to estimate the kinetic parameters for hydrolysis of maltose at 30ºC, according to a Michaelis-Menten equation, with inhibition by the product. Runs with the immobilized enzyme enable us to estimate dextrin diffusivities in silica, assuming that the inherent kinetic parameters are equal to the intrinsic ones. The values of kinetic and mass transfer parameters are: K3 = 0.90x10-5 g/U.s, Km = 0.61x10-3 g/ml, Ki = 1.23x10-1 g/ml, DMs = 0.50x10-6 cm2 /s and DGs = 0.55x10-6 cm2 /sBrazilian Society of Chemical Engineering1997-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321997000400007Brazilian Journal of Chemical Engineering v.14 n.4 1997reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66321997000400007info:eu-repo/semantics/openAccessGonçalves,L.R.B.Giordano,R.L.C.Giordano,R.C.eng1998-10-06T00:00:00Zoai:scielo:S0104-66321997000400007Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:1998-10-06T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false
dc.title.none.fl_str_mv EFFECTS OF DIFFUSION ON THE KINETICS OF MALTOSE HYDROLYSIS USING GLUCOAMYLASE IMMOBILIZED ON MACROPOROUS SILICA
title EFFECTS OF DIFFUSION ON THE KINETICS OF MALTOSE HYDROLYSIS USING GLUCOAMYLASE IMMOBILIZED ON MACROPOROUS SILICA
spellingShingle EFFECTS OF DIFFUSION ON THE KINETICS OF MALTOSE HYDROLYSIS USING GLUCOAMYLASE IMMOBILIZED ON MACROPOROUS SILICA
Gonçalves,L.R.B.
Maltose hydrolysis
dextrin diffusivity
kinetic parameters
title_short EFFECTS OF DIFFUSION ON THE KINETICS OF MALTOSE HYDROLYSIS USING GLUCOAMYLASE IMMOBILIZED ON MACROPOROUS SILICA
title_full EFFECTS OF DIFFUSION ON THE KINETICS OF MALTOSE HYDROLYSIS USING GLUCOAMYLASE IMMOBILIZED ON MACROPOROUS SILICA
title_fullStr EFFECTS OF DIFFUSION ON THE KINETICS OF MALTOSE HYDROLYSIS USING GLUCOAMYLASE IMMOBILIZED ON MACROPOROUS SILICA
title_full_unstemmed EFFECTS OF DIFFUSION ON THE KINETICS OF MALTOSE HYDROLYSIS USING GLUCOAMYLASE IMMOBILIZED ON MACROPOROUS SILICA
title_sort EFFECTS OF DIFFUSION ON THE KINETICS OF MALTOSE HYDROLYSIS USING GLUCOAMYLASE IMMOBILIZED ON MACROPOROUS SILICA
author Gonçalves,L.R.B.
author_facet Gonçalves,L.R.B.
Giordano,R.L.C.
Giordano,R.C.
author_role author
author2 Giordano,R.L.C.
Giordano,R.C.
author2_role author
author
dc.contributor.author.fl_str_mv Gonçalves,L.R.B.
Giordano,R.L.C.
Giordano,R.C.
dc.subject.por.fl_str_mv Maltose hydrolysis
dextrin diffusivity
kinetic parameters
topic Maltose hydrolysis
dextrin diffusivity
kinetic parameters
description In this work the effects of diffusion on the hydrolysis of maltose using glucoamylase immobilized on macroporous silica at 30o C are studied. Two different kinds of experimental assays are carried out: one using free enzyme and the other using an enzyme covalently linked to macroporous silica, preactivated with glutaraldehyde. Free enzyme assays are used to estimate the kinetic parameters for hydrolysis of maltose at 30ºC, according to a Michaelis-Menten equation, with inhibition by the product. Runs with the immobilized enzyme enable us to estimate dextrin diffusivities in silica, assuming that the inherent kinetic parameters are equal to the intrinsic ones. The values of kinetic and mass transfer parameters are: K3 = 0.90x10-5 g/U.s, Km = 0.61x10-3 g/ml, Ki = 1.23x10-1 g/ml, DMs = 0.50x10-6 cm2 /s and DGs = 0.55x10-6 cm2 /s
publishDate 1997
dc.date.none.fl_str_mv 1997-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321997000400007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321997000400007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0104-66321997000400007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
dc.source.none.fl_str_mv Brazilian Journal of Chemical Engineering v.14 n.4 1997
reponame:Brazilian Journal of Chemical Engineering
instname:Associação Brasileira de Engenharia Química (ABEQ)
instacron:ABEQ
instname_str Associação Brasileira de Engenharia Química (ABEQ)
instacron_str ABEQ
institution ABEQ
reponame_str Brazilian Journal of Chemical Engineering
collection Brazilian Journal of Chemical Engineering
repository.name.fl_str_mv Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)
repository.mail.fl_str_mv rgiudici@usp.br||rgiudici@usp.br
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